Objective To investigate the role of nitric oxide(NO)in the spinal cord sensitization in a rat model of neuropathic pain.Methods Thirty-two male adult Wistar rats weighing 200-300 g were randomly divided into 4 groups with 8 animals in each group:group Ⅰ sham operation;group Ⅱ L-NAME pretreatment + sham operation;group Ⅲ CCI and group Ⅳ L-NAME pretreatment + CCI.Neuropathic pain was induced by chronic constrictive injury(CCI).The animals were anesthetized with intraperitoneal pentobarbital 40 mg/kg.The right sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1 mm intervals with 3-0 silk thread.In group Ⅰ and Ⅱ the sciatic nerve was exposed but not ligated.In group Ⅲ and Ⅳ L-NAME 250 μg in normal saline 10 μl was injected into subarachnoid space over 30 seconds at 15 min before sham operation or CCI.The paw-withdrawal threshold to nociceptive thermal stimuli(PWTT)was measured before operation(baseline)and on the 3rd day after operation.Four rats in each group were killed on the 4th and 7th day after operation,and lumbar segment of the spinal cord(L4-5)was removed for determination of expression of pCREB in the dorsal horn of spinal cord by immuno-histochemical technique.Results The paw-withdrawal threshold to nociceptive thermal stimuli(PWTT)was significantly decreased on the 3rd day after operation as compared with the baseline before operation in all 4 groups.The PWTT on the 3rd day after operation was significantly higher in group Ⅰ,Ⅱ and Ⅳ than in CCI group(group Ⅲ),but there was no significant difference in the PWTT after operation among the 3 groups(Ⅰ,Ⅱ,Ⅳ).The expression of pCREB in the dorsal horn neurons of spinal cord was significantly upregulated in CCI group (Ⅲ) as compared with the other 3 groups.Conclusion Spinal cord NO induces hyperalgesia in neuropathic pain through release of pCREB in spinal cord dorsal horn.

Objective To detection analysis of blood infectious disease markers in patients before blood transfusion or before surgery .Methods HBsAg ,anti-HBs ,HBeAg ,anti-HBe ,anti-HBc ,anti-HCV ,anti-HIV ,TP-Ab were detected for 3 542 cases of se-rum using chemiluminescence immunoassay(CLIA) and ELISA .Results For HBsAg ,there were 704 cases of positive among 3 542 cases ,the proportion was 19 .8% ;For anti-HCV ,there were 52 cases of positive among 3 032 cases ,the proportion was 1 .72% ;For anti-HIV ,there were 3 cases of positive among 3 049 cases ,the proportion was 0 .09% ;For TP-Ab ,there were 200 cases of positive among 3 153 cases ,the proportion was 6 .34% .The infection rate was different in different gender of HBV ,anti HCV ,anti HIV and TP-Ab .But for HBV ,anti-HCV and TP-Ab ,the detection rate of male was higher than female(P<0 .05);The infection rate was different in different age of HBV ,anti HCV ,anti HIV and TP-Ab different age .The detection rate of HBV and Anti-HCV in 21-40 years old was the highest ,They were 31 .9% and 2 .33% ;The detection rate of TP-Ab increases with age increased ,more than 60 years old was the highest ,up to 7 .55% .Detection of the above two methods ,for HBsAg ,there were 51 cases did not match ,the proportion was 1 .44% ;for anti-HCV ,there were 1 case did not match ,the proportion was 0 .03% ;for Tp-Ab ,there were 3 cases did not match ,the proportion was 0 .1% ;for anti-HIV ,CLIA agrees well with ELISA ,there were 3 cases of positive .The samples that did not match were detected by the third party verification of the reagent .The result displayed that CLIA was more accurate . Conclusion CLIA is a high-sensitivity detection method which could effectively avoid positive specimens missed ,detection of infec-tious disease markers in patients before transfusion or before surgery by CLIA had important significance .

Objective To study the effect of massive blood transfusion program(MTP) in early massive blood transfusion and preventing coagulopathy.Methods Fifty cases of massive blood transfusion were clinically collected and performed the statistical analysis and processing before and after intervention according to the blood transfusion scheme of MTP.The statistical comparison was performed by using the data before and after intervention.Results PLT was decreased with the increase of transfusing of RBC.After transfusing 15 U of RBC,the PT test,INR and APTT were increased significant(P＜0.05).After transfusing 20 U of RBC,the Fib was decreased significant(P＜0.05).The MTP had statistical difference between before and after intervention(P＜0.01).Conclusion The MTP application has an important significance to prevent the occurrence of coagulopathy and evaluate the coagulation status.

ObjectiveTo study the effect of a fast-track surgery (FTS) program on the clinical outcomes in cirrhotic patients with portal hypertension.Methods42 cirrhotic patients with portal hypertension were randomly allocated into the FTS group (n=21) and the conventional therapy control group (n=21).The postoperative time to first defecation,hospital stay,hospitalization cost,and postoperative complications were compared between the two groups.ResultsCompared with the control group,the postoperative time to first defecation was significantly shorter in the FTS group (P=0.0287).Furthermore,the postoperative hospital stay was significantly shorter in the FTS group than the control group (P=0.002).Additionally,hospitalization cost was significantly lower in the FTS group than the control group (P＜0.001).The postoperative complication was also significantly different between the two groups (7.15 ％ vs 21.5 ％,P=0.001).ConclusionA FTS program contributed to better postoperative rehabilitation in cirrhotic patients with portal hypertension.

To investigate whether a series of water-soluble cross-linked chitosan derivates synthesized in the guide of imprinting technology could be used as a uranium chelating agent to protect cells exposed to depleted uranium (DU), the imprinted chitosan derivates with high UO2(2+) chelating ability were screened, and cell model of human renal proximal tubule epithelium cells (HK-2) exposed to DU (500 micromol.L-1) was built, chitosan derivates (400 mg.L-1 ) was added to test group and diethylenetriaminepentaacetic acid (DTPA, 50 mg.L-1) was added to positive control group. The results showed that three Cu2+ imprinted chitosan derivates had higher uranium chelating ability (>49 microg.mg-1) than chitosan and non-imprinted chitosan derivates. Compared to the cells exposed to DU only, survival of cells in group added chitosan derivates rose up significantly (increased from 57.3% to 88.7%, and DTPA to 72.6%), and DU intracellular accumulation decreased, membrane damage and DNA damage also eased. Among the imprinted chitosan derivates, Cu2+ imprinted penta dialdehyde cross-linked carboxymethyl chitosan (Cu-P-CMC) was the best, and better than DTPA. From ultrastructure observation, the DU precipitates of test group added Cu-P-CMC were most grouped in a big hairy clusters in a string together outside cells. It is possible that the DU-chitosan derivates precipitates are too big to enter into cells, and from this way, the DU uptake by cells decreased so as to detoxication.

ObjectiveTo explore the correlation of resilience and coping style,subjective quality of life of young children of schizophrenia patients.Methods 120 adolescents whose parents were clearly diagnosed with schizophrenia wcrc chosen for the study.They were ranked according their performances by Resilience Scale for Adolescents.32 were classified into the group with high resilience and another 32 were assigned to the group with low resilience by applying the criteria that 27％ of the sample size should be differentiated.Then,children in highscore group and low-score group were administered by Simple Coping Style Questionnaire (SCSQ) and Children 's Subjective Quality of Life Questionnaire(ISLQ).ResultsResilience score of research group were lower than those of normal high school students(P＜ 0.05 or 0.01 ).The score and total score of SCSQ positive coping dimensionality of research group who had high resilience score were higher than those of low resilience score group(P ＜ 0.01 ) ;score of passive coping dimensionality were lower than those of low resilience score group (P ＜ 0.01 ).In addition to dimensionality of living environment,the score of dimensionality of cognitive and affective element and the total score of ISLQ of research group who had high resilience score were higher than those of low resilience score group.The total score of resilience was in significant by positive correlation with the total score of ISLQ,cognitive and affeetive element,the total score of SCSQ,the score of positive coping dimensionality.The total score of resilience was in negative correlation with the score of passive coping dimensionality (P ＜ 0.01 ) ;dimensionality of the target focus of resilience was not correlative with cognitive element of ISLQ,the score of negative coping dimensionality of SCSQ,scores of other dimensionality of resilience were in significant correlation with score of other dimensionality of ISLQ and SCSQ(P ＜ 0.01 or 0.05 ).ConclusionYoung children of schizophrenia have poor resilience; research group who has high resilience score adopts more positive coping and has better subjective quality of life ; resilience is positive correlation with positive coping and subjective quality of life.

24 hours) PCI. Results Among 1013 patients enrolled in the SUNDAY registry, 438 (male 74.8%, unstable angina 94.1%) received PCI after CAG, 35 patients received PCI within 24 hours [(1.0?0.0) day, group I], and 403 after 24 hours [ (7.5 ? 7.3) days, group II] of hospitalization (P

Objective To assess the effect of anemia on long-term outcomes in patients with acute coronary syndrome(ACS) undergoing pereutaneous coronary intervention(PCI). Methods In 3136 patients presenting with ACS,636 patients were anemic. The clinical features, mortality and major cardiocerebral events including non-fatal acute myocardial infaret,revascularization and non-fatal cerebral stroke were compared in patients with or without anemia. The average follow-up period was 550 days. Results Anemic patients were older and had a higher percentage of comorbidities compared with nonanemic cohorts. Compared with nonanemic patients, anemic patients had higher mortality (4.7% versus 1.5% ,P <0. 001) and a higher major adverse end point events,including nonfatalmyocardial infarction, stroke and revaseularization (14.2% versus 11.0%, P = 0.032). After adjustment for comorbidities, anemia was associated with a higher risk of mortality after percutaneous coronary intervention (adjusted hazard rate ratioRR2. 166 ;95% CI 1. 298-3. 612 ;P =0.003). Conclusion Anemia before PCI is an independent factor for predicting the long-term mortality of ACS.

Objective To evaluate the effect of clopidogrel premedication on in-hospital major adverse cardiovascular and cerebral events (MACCE) and bleeding outcomes before coronary artery bypass graft surgery (CABG). Method A total of 2021 patients who underwent CABG from July 2003 to September 2005 were divided into either clopidogrel ( n = 479) or no clopidogrel (1542) group before CABG. Patients with clopidogrel administration ( n = 479) were subdivided into < 5 d ( n = 154) ,5～7d(n = 183)and >7d(n = 142) group according to timing of clopidogrel withdrawal before surgery. In-hospital MACCE and perioperative bleeding outcomes were analyzed among groups. Results Patients who took clopidogrel before surgery had nonsignificantly rates of bleeding and in-hospital MACCE compared with those patients not administered clopidogrel. No differences were found about the incidence of total bleeding,minor bleeding,transfusions of red blood cells,fresh frozen plasma,whole blood and in-hosptial MACCE among three subgroups.The < 5 d group had higher incidence of major bleeding and more platelets transfusions than 5 ～ 7 d [47.8% vs. 31.9%,P < 0.017; (0.08 ±0.38) U vs. (0.00±0.00) U,P <0.017,respectively]and >7 d group [47.8% vs. 20.3%,P <0.017; (0.08±0.38) U vs. (0.00±0.00) U,P <0.017,respectively). However,there were no significant differences between 5 ～ 7 d and > 7 d group ( P > 0.05). Conclusions Gopidogrel administration before CABG does not increase the incidence of in-hospital MACCE events. However,the perioperative risk of bleeding will rise if the patients withhold clopidogrel less than five days before surgery.

OBJECTIVE: To validate the expression of Cu/ZnSOD and PLUNC protein in chronic sinusitis and nasal polyp tissue by immunohistochemistry. METHOD: Thirty-four nasal polyps, 30 chronic sinusitis and 18 control cases were chosen. All samples were stained with SP immunohistochemical method to examine Cu/ZnSOD and PLUNC protein respectively. RESULT: (1) Interestingly, we found the positive intensity of Cu/ZnSOD and PLUNC protein were the same in one sample, and they were both expressed in nasal epithelium mucosae, glandular epithelium and goblet cells; (2) We calculated that the constituent ratio among nasal polyps, chronic sinusitis and normal nasal mucosa was significant difference (chi2 = 30.689, P<0.01). The positive intensity of nasal polyps was mainly "+", and the positive intensity of chronic sinusitis was mainly "+++", and the positive intensity of normal nasal mucosa was mainly "++". CONCLUSION Both Cu/ZnSOD and PLUNC protein are important part of the innate defense mechanism in nasal mucosa. Once the nasal mucosa is irritated by infectious agents or chemical factors, nasal epithelium mucosae, glandular epithelium and goblet cells will proliferate reactively, and then produce antiinflammatory agents such as Cu/ZnSOD and PLUNC protein to defend inflammation.
Adolescent ; Adult ; Aged ; Case-Control Studies ; Chronic Disease ; Female ; Glycoproteins ; metabolism ; Humans ; Inflammation ; Male ; Middle Aged ; Nasal Mucosa ; metabolism ; pathology ; Nasal Polyps ; metabolism ; pathology ; Phosphoproteins ; metabolism ; Sinusitis ; metabolism ; pathology ; Superoxide Dismutase ; metabolism ; Young Adult