OBJECTIVE To evaluate the benefits,if any, of thyroidectomy using the harmonic scalpel versus conventional thyroidectomy. METHODS The clinical data of 34 consecutive patients underwent thyroid nodule enucleation or lobectomy in one year using the harmonic scalpel were recorded. The results were compared with that of the 34 case-control patients in the same period using conventional techniques. RESULTS The average incision length for those underwent thyroidectomy with the harmonic scalpel and conventional techniques was 3.8cm and 6.9cm respectively. Drains were not used in the harmonic scalpel group compared with average of 47ml drainage in the control group. The average inpatient cost was 7205 RMB for the harmonic scalpel group versus 8054 RMB for the control group(P=0.137). The average postoperative length of hospitalization was 3.5 days for the harmonic scalpel group versus 4.1 days for the control group (P=0.228). The average time of anesthesia was 95 minutes for the harmonic scalpel group compare with 81 minutes for the control group (P=0.018). One postoperative hoarseness occurred in each group. Two patients had mild skin burn in the harmonic scalpel group. No other complications were enumerated(x2=0.273,P=0.602). CONCLUSION The harmonic scalpel is a safe alternative for thyroidectomy. Compare with conventional thyroid surgery, this technique offers cosmetic benefit and less trauma for patient with thyroid nodules.

Objective: To determine the overlapping area of the femoral condyle facet on which the patellofemoral and tibiofemoral joint surfaces move and to measure the pressures on such area in order to better design the length of femoral trochlea prosthesis in patellofemoral arthroplasty. Methods: Eight fresh cadaver knees were mounted on Instron 8501 biomechanics tester,and simulated physiologically weight-bearing situation of knee from standing to flexion position. Such overlapping area was drawed up with stain method and the contact pressures were measured with Fuji pressure film. Results: There existed an overlapping contact area on the femoral condyle facet within the patellofemoral contact area when knee flexion was 75? or larger. The tibiofemoral contact pressure was higher than the patellofemoral pressure in the area. Conclusion: The femoral trocher prosthesis should cover the whole contact area of patellofemoral joint.

Objective To analyze anemia in patients with chronic heart failure changes in relevant indicators.Methods 48 chronic heart failure patients(observation group)and 48 healthy people(control group).were erolled the peripheral blood red blood cell count(RBC),hemoglobin(Hb),hematocrit(Hct)and other anemia-related indicators were measured.Results The results of the observation group RBC,Hb,Hct,were(3.37 ±0.49)× 1012/L,(103.64±14.33)g/L,(0.31 ±0.08)respectively,the control group were(4.64 ±0.51)× 1012/L,(121.33 ±17.69)g/L,(0.37 ± 0.07),between the two groups were statistically significant(t =4.46、3.06、4.18,P =0.02,0.04,0.04);treatment,symptoms of heart failure in observation group mitigation,RBC is(3.76 ± 0.58)× 1012/L,compared with the control group the difference was significant(t =0.48,P =0.03); observation group Hb,Hct were (123.77 ± 17.26)g/L,(0.37 ± 0.05),and were not significantly different with the amtrol gronp(P =0.06,0.11).Conclusion Elderly patients with anemia associated with heart failure were lower than healthy people,heart failure and anemia had,a significant correlation with aremia.

Objective To compared the posterior slop between tibial plateau and meniscus in Chinese population for determining the angle of tibial osteotomy during total knee replacement(TKA).Methods Posterior slop of tibial plateau and meniscus in sixty Chinese volunteers were measured on sagital view of MRI image.Results The values of posterior slop of tibial plateau and meniscus were 9.87??1.56?and 0.96??0.09?,respectively.The two angle revealed remarkable difference (P

Objective To study changes of serum IL-17 and IL-35 levels in patients with heart failure. Methods 60 patients with heart failure (observation group)were selected as research subjects.60 patients accord-ing to different severity were divided into acute period heart failure (34 cases)and stable stage heart failure (26 ca-ses);60 patients graded according to the NYHA standards were divided into 24 cases of heart failure with grade Ⅱ, 20 cases of grade Ⅲ ,16 cases of grade Ⅳ.According to the different primary diseases :expansion cardiomyopathy group (20 cases in group A),the coronary heart disease group (group B,24 cases),hypertensive heart disease group (group C,16 cases).During the same period,42 healthy elderly people in our hospital were selected as control group. The serum IL-17,IL-35 levels were tested,and the serum IL-17,IL-35 levels in patients with heart failure were ana-lyzed.Results Serum level of IL-17 in the observation group was higher than the control group,and the difference was significant [(15.61 ±4.02)pg/mL vs (9.49 ±3.96)pg/mL,t =9.018,P <0.01].Serum level of IL-35 in the observation group was significantly lower than that of the control group,and the difference was significant[(52.78 ± 4.29)pg/mL vs (61.49 ±4.81)pg/mL,t =11.963,P <0.01].The level of serum IL-17 in acute stage of patients with heart failure was higher than that of stable heart failure,and the difference was significant (t =6.278,P <0.01);IL-35 level in serum of patients with heart failure in acute phase was lower than that of stable heart failure,the difference was significant (t =9.529,P <0.01).With the increase in heart failure grade,serum IL-17 level showed a rising trend,and the differences among three groups had statistical differences (F =6.098,P <0.01);serum IL-35 level decreased,and the differences among three groups had statistical differences(F =8.978,P <0.01).The serum IL-17 level of A group was higher than that in B group and C group,there were significant differences (F =6.096, P <0.01),the serum IL-17 level between B group and C group had no statistical difference (t =0.172,P >0.05). The serum IL-35 level of A group was lower than that of B group and C group,there were significant differences (F =8.978,P <0.01),the serum IL-35 level between B group and C group had no statistical difference (t =0.208,P >0.05).Serum IL-17 and serum IL-35 level was negatively correlated (r =-0.429,P =0.009).Conclusion High expression of IL-17 in elderly patients with heart failure,while IL-35 decreased in elderly patients with heart failure, IL-17,IL-35 are closely related to the senile congestive heart failure and the severity of illness.Serum IL-17 is nega-tively correlated with the level of serum IL-35.

qualitative and quantitative analysis of solid depolymerization products for supercritical methanolysis of ploy(ethylene terephthalate) polyester (PET) were studied by means of reversedphase high performance liquid chromatogr aphy on ZorbaxC8(4.6mm×250mm) with 70%(V/V) methanol as mobile phase. The results showed that the solid depolymerization products were mainly composed of dimethyl terephthalate (DMT), methyl(2hydroxyethyl)terephthalate (MHET), bis(hydroxyethyl)tere phthalate (BHET), dimers and oligomers, which could be separated effectively under the above chromatographic conditions. The calibration curves of DMT and BHET were linear in the range of 1.0～45 mg/L (n=8),r=0.9998～1.0000 and RSD＜2.8%. The determination limits of DMT and BHET were 4.0×10-4μg and 6.0×10-4μg respectively. 

Objective To explore the change of function and expression of hematopoietic lineage cell specific protein-1 (HS1) and phosphorylated HS1 (p-HIS) and factors devoting to HS1 phosphorylation in platelet with sepsis.Methods Plasma with rich platelet was collected from 150 sepsis patients and 50 healthy subjects, and comparison of platelets adhesion and aggregation were detected by micro-pore method and platelet aggregation instrument.Meanwhile the ATP concentrations of washed platelet of two groups were detected by the kit to compare release reaction.And then total HS1 (t-HIS) and p-HS1 of platelet from two groups were compared by using western blot.Afterwards the specific inhibitors of Src and Syk were used to verify the HS1 activation regulated by Src and Syk in LPS-induced cell model.Results The significant differences were present between healthy subjects and sepsis patients in platelet counts, platelet distribution width (PDW) and mean platelet volume (MPV) (P ＜ 0.01).The data showed the sepsis patients had greater ability than healthy subjects in adhesion, aggregation and release reaction.Meanwhile the platelets of sepsis patients had higher concentration of t-HS1 and p-HS1 than healthy subjects, and the specific inhibitors of Src and Syk , PP2 and piceatannol, inhibited the increase in p-HS1 in LPS-induced cell model.Conclusions Function of platelet is closely related to HS1 in sepsis and it will be a target for sepsis therapy.

AIM:To investigate the effect of nuclear factor kappa B(NF-?B)on proliferation,apoptosis and the expression of TGF-?1 in airway smooth muscle cells(ASMCs).METHODS:Cultured ASMCs were divided into three groups and stimulated with or without TNF-? and pyrrolidine dithiocarbamate(PDTC)in vitro.Reverse transcriptase-polymerase chain reaction(RT-PCR)was used to detect the expression of TGF-?1 mRNA.The location and protein expression of PCNA and Bcl-2 were observed by immunocytochemical staining.The protein expression of TGF-?1 and NF-?B was detected by Western blotting.The proliferation and apoptosis of ASMCs were also observed.RESULTS:The activity of NF-?B in TNF-? group was significantly higher than that in control group and TNF-? plus PDTC group,respectively(P

ObjectiveTo explore the effect of TLR4,PI3K and NF-κB on the migration of asthmatic airway smooth muscle cell(ASMCs) induced by airway epithelial cells.MethodsPrimary ASMCs were cultured by the method of cell digestion.Cell culture supernatant of RTE cells were collected by TNF-α stimulation of epithelial cells.Detected the IL-8 and RANTES levels in the supernatant.The effect of TLR4/PI3K-related signaling molecules on the migration of asthmatic ASMCs induced by epithelial cells were detected by Modified Boyden chemotaxis chamber with anti-TLR4 antibody,Wortmannin and PDTC drugs as a tool.ResultsThe levels of IL-8 and RANTES in the supernatant of TNF-αgroups were significantly increased,and that in the 20 ng/ml group was significantly higher than other groups ( P＜0.01 ).Compared with control group,the transmembrane migration of asthmatic ASMCs from other groups was significantly increased (P＜0.01 ).The transmembrane migration of asthmatic ASMCs from treated groups was significantly increased than asthma group (P＜0.01).The migration of asthmatic ASMCs from TNF-α+anti-TLR4 antibody group,TNF-α+Wortmannin group and TNF-α+Wortmannin+PDTC were significantly decreased than that of TNF-αgroup( P＜0.01 ).The migration of asthmatic ASMCs from TNF-α+Wortmannin+PDTC were significantly decreased than that of TNF-α+Wortmannin group (P＜0.05).ConclusionTLR4/PI3K-related signaling molecules involved in the transmembrane migration of asthmatic ASMCs induced by airway epithelial cells and may be one of the mechanisms of airway remodeling of asthma.

As the complexity of samples and experimental processes, the repeatability of mass spectrometry experiments is still not satisfactory, the results of peptide identification and quantification show high randomicity), the probability of peptide being detected by mass spectrometry in proteome research, especially in quantitative proteomic study, has received much attention. Therefore, a lot of experimental researches have been done, as well as a number of computational prediction methods have been developed. In this article, we summarized the important factors impacting the peptide detectability, investigated the existing prediction methods) and reviewed their applications in experimental study.