Prednisolone-induced zebrafish osteoporosis model was used to explore the bone-strengthening effect of Jie-Gu-Tang (JGT). Zebrafish larvae of 5 days post fertilization (d.p.f.) were co-exposed with 25 μmol·L-1 pred-nisolone and a series of JGT solutions with a range of concentrations (0.025, 0.25, 2.5, 25 and 100 mg crude herb per liter). The 25 μmol·L-1 prednisolone was selected as the model group. Etidronate disodium (15 and 30 mg·mL-1) with 25 μmol·L-1 prednisolone was used as the positive group. And 0.5% DMSO was used as the vehicle control group. All groups were incubated in 24-well plates (28.5℃) until 10 d.p.f. Zebrafish skeleton at 10 d.p.f. was anes-thetized and fixed for staining with alizarin red. Quantitative analysis of the stained area was performed by microscop-ic inspection and digital imaging methods to reflect the amount of zebrafish head skeleton mineralization. The results showed that prednisolone group at 25 μmol·L-1 concentration can obviously decrease the staining area and the stain-ing optical density values when compared with the vehicle control group (0.5% DMSO). Compared with the model group, both etidronate disodium (15 and 30 mg·mL-1) and JGT (2.5, 25 and 100 mg crude herb per liter) can in-crease the mineralized matrix and integrated optical density (IOD) of zebrafish head skeleton significantly with dose-effect relationship. It was concluded that zebrafish osteoporosis model was successfully used in the evaluation on bone loss prevention and bone formation promotion of JGT, which provided basis for the reliability and reasonability of zebrafish model.

Objective To explore clinical application effects of hands-off method among puerperas delivered through vagina. Methods To utilize retrospective study design, a total of 1 040 puerperas, who could not received natural labour from July to November 2013, were selected as observation group by convenient sampling method and underwent hands-off method to deliver children, while 1 005 prerperas were taken deliver technical protection of perineum from July to November 2010 as control group. We compared puerperas′delivery conditions and injury of perineum, and observed and analyzed the influencing factors of puerpera′s episiotomy and perineal laceration. Results No incidences of Ⅲ and Ⅳ degree perineal laceration and obstetric anal sphincter injury happened. The rate of puerpera′s episiotomy in the observation group was 25. 38% compared with 13.93% in the control group (P <0.01). The Ⅱ degree of perineal laceration was 48. 85% in the observation group lower than 82. 09% in the control group (P<0. 01). Comparing injury of perineum between two groups, the medium injury of observation group (Ⅱdegree of perineal laceration and episiotomy, 74. 23%) was lower than 96. 02% puerperas of control group (P <0. 01). The rate of vaginal wall hematoma in the observation group was 0. 77% lower than 4. 68% in the control group (P<0. 01). The length of second stage of labor and length of perineum wound suture were shorter than those of the control group (P<0. 05);the amount of bleeding 2 h after labor in two groups had no statistical significance (P>0. 05). Regression analysis showed that, maternal age, analgesia, newborn weight were the influence factors of lateral episiotomy (P <0. 05);maternal age, weeks of gestation, analgesia, newborn weight were the influence factors of perineal laceration (P<0. 05). Conclusions The hands-off method to deliver child can elevate the perineal laceration degree of perineum, reduce the length of the second stage labor, and shorten the length of perineal wound suture.

Objective To explore the application effects of free body position with delivery vehicles in the first late stage of labor and the second stage of labor, and to choose a proper delivery position for parturient women.Methods Totally 208 primiparas who had term and spontaneous labor in single pregnancy and head position between August and September 2015 in a maternal and child health hospital were selected and allowed to get out of bed at their will in the first late stage of labor and the second stage of labor. Midwives accompanied and instructed parturient women to use free body position with delivery vehicles, and when head visible on vulval gapping for 2 cm×2 cm, assisted the parturient women for conventional delivery on delivery beds. And 175 primiparas awaiting delivery using traditional horizontal position and fowler position between August and September 2014 were selected as a control group. Then their time of the second stage of labor, intrapartum hemorrhage, perineal laceration or episiotomy, forceps delivery, Apgar scores and body weight of neonates were compared.Results There was no statistical difference in the second stage of labor, intrapartum hemorrhage, perineal episiotomy and forceps delivery between the parturient women using free body position with delivery vehicles and those using traditional horizontal position and fowler position in the first late stage of labor and the second early stage of labor (P>0.05). With the use of free body position with delivery vehicles, the parturient women's VAS was decreased from (8.11±1.59) to (6.49±1.90) (t=13.16,P<0.001).Conclusions Based on the evaluation of engagement, the use of free body position with delivery vehicles in the first late stage of labor until their delivery on bed in the second stage of labor is safe for parturient women and helps to reduce their delivery pain substantially.

Objective To study the effects of neonatal bilateral and unilateral early sucking on the secretion time and the amount of colostrum.Methods A total of 300 cases with normal full-term delivery and nipple condition were selected and randomly divided into two groups:bilateral early sucking group and unilateral early sucking group.Women in the two groups were assisted by a midwife within 1 h after birth to implement early suction,with women of bilateral early sucking receiving breast sucking 15 minutes on each side and unilateral early sucking group 30 minutes just on one side.Maternal lactation′s initiating time and amount of lactation of two groups after postpartum 6 hours,24 hours,48 hours and 72 hours were analyzed.Results There was no difference of lactation initiating time in bilateral early sucking group and unilateral early sucking group (P >0.05).After 6 hours,lactation in the 2 groups had no difference (P >0.05).After 24 hours,48 hours and 72 hours,number of women with sufficient lactation in bilateral early sucking group was greater than unilateral early sucking group (P <0.05).Conclusions Bilateral early sucking is more favorable than unilateral early sucking postpartum for lactation amount.

Objective:To investigate the post competency of "Academic-Practical" of clinical nursing teachers, and analyze related influencing factors.Methods:A total of 312 "Academic-Practical" and "Non-Academic-Practial" clinical nursing teachers from The Affiliated Hospital of Southwest Medical University were surveyed by the Clinical Nursing Teacher Post Competency Evaluation Questionnaire. SPSS 20.0 software was used for t test, chi-square test and rank sum test. Results:The average self-evaluation scores of post competency of "Academic-Practical" clinical nursing teachers were (4.26±0.41) points, which were higher than those of the "Non-Academic-Practical" teachers [(3.19 ±0.50) points], showing good post competency. There were significant differences in the scores of professional quality (17.39±1.54), professional attitude (21.75±2.21), professional ability (21.14±2.31), teaching ability (50.39±5.93), interpersonal coordination ability (25.57±3.04), and personality characteristics (17.27±2.04) between the "Academic-Practical" and "Non-Academic-Practical" teachers (all P<0.01). And there were significant differences in self-evaluation post competency scores of "Academic-Practical" teachers in "with or without teacher qualification certificate" ( P=0.001), "whether she/he is the backbone of the department" ( P=0.002), degree ( P=0.001), age ( P<0.001), positional title ( P<0.001) and working year ( P<0.001) (all P<0.01). But there was no significant difference in gender ( P=0.735) and "whether she/he is a specialized nurse" ( P=0.335). Conclusion:"Academic-Practical" and "Non-Academic-Practical" medical teachers should take the post competency as the core orientation, adopt the "Ladder" mode of training and management, and constantly improve the training plan of post competencey.