Objective:To understand the awareness of students of medical colleges sperm behavior, attitudes and prac-tical factors. Methods:Used convenient sampling method to select 300 college students of 4 medical colleges in Shanghai conducted a questionnaire survey and used Epidata 3. 1 and SPSS 18. 0 to count and analyse. Results: 251 question-naires. 39. 8% of respondents were willing to sperm, the primary reason was considered to be an act of charity, helping others;willing sperm donors only distributed among schools statistically significant (P<0. 05) proportions, and for the various professional and grades , showed no significant difference ( P>0 . 05 ) . Conclusion:Students of Shanghai Medical Colleges sperm voluntary rate was not high, so the survey analyzed related factors affecting sperm donation and put forward practical proposals to provide reference data and methods for sperm bank sperm recruiting for volunteers in the universities.