Objective To explore diagnosis and operative treatment of primary cholecy stocarcinoma. Methods One hundred and thirty-six cases of biliary carcinoma were analysed for their clinical and pathological data and selection of the method of operation.Results The prognosis of late-staged cases was very bad.B-US and CT are still the most helpful means for clinical diagnosis.Conclusion The early diagnosis and treatment of primary cholecystocarcinoma are of utmost importance.

To evaluate the influence of combined endurance and strength training on the knee flexors and extensors strength of Chinese army male recruits. At the beginning and end of their 4 weeks′ combined endurance and strength training, twenty recruits received isokinetic dynamometer test for flexors and extensors of both knees with the Cybex 6000 isokinetic dynamometer. after training, there was a significant increase in the peak torque values, torque acceleration energy, and endurance ratio of flexors of both knees at two angular velocities (60?/s,180?/s). Meanwhile, the torque acceleration energy and endurance ratio were increased in the extensors of both knees at two angular velocities . It suggested that four weeks of combined endurance and strength training for the recruits is effective in terms of increasing muscular strength, explosive force and muscular endurance of knee flexors and extensors. More loads should be applied to strengthen the strength of the extensors.

Objective To evaluate the influence of intensified and step-by-step training on the strength of the knee flexors and extensors in Chinese army male recruits. Methods At the beginning and end of their 8-week endurance training, 160 recruits received isokinetic dynamometer tests for both knees flexors and extensors with the Cybex-6000 isokinetic dynamometer. Results After endurance training, there was a significant increase in the peak torque values, torque acceleration energy, and endurance ratio of both knee flexors at two angular velocities (60 /s,180 /s). Conclusion Eight weeks of recruit intensified and step-by-step training for male recruits is sufficient for increasing muscular strength, explosive force and muscular endurance of knee flexors and extensors.

To evaluate the influence of combined endurance and speed training on the knee flexors and extensors strength of army male recruits. At the beginning and end of their 4 week combined endurance and speed training, twenty recruits received isokinetic dynamometer test of flexors and extensors of both knees with the Cybex 6000 isokinetic dynamometer. The results showed: that there was a significant increase in the peak torque values, torque acceleration energy and endurance ratio of left knee flexors at high angular velocities (180?/s) after training. Meanwhile, the peak torque and endurance ratio were increased in left knee extensors at high angular velocities (180?/s),and the peak torque and torque acceleration energy were increased in right flexors at high angular velocities(180?/s). These data suggest that four weeks of combined endurance and speed training for recruits is not sufficient fo increase muscular strength, explosive force or muscular endurance of knee flexors and extensors.

Objective To investigate the change of peripheral blood CD4+CD25+ regulatory T cells (Tr) in patients with idiopathic thrombocytopenic purpura (ITP), and to analyze its role in the pathogenesis of ITP.Methods Anticoagulated venous blood was collected from ITP patients (ITP group,n=35) and healthy controls (healthy control group,n=35). T lymphocytes were isolated and purified with human CD3+ T cell enrichment columm. The percentage of peripheral blood CD4+CD25+ regulatory T cells was detected by immunofluorescence staining (PE-anti-CD4 monoclone antibody and FITC-CD25 monoclone antibody) and bicolor flow cytometry by CD25/CD4 gating.Results The number and constituent ratio of CD4+CD25+T cell were significantly lower in ITP group than those of healthy control group (P<0.05).Conclusion There is peripheral blood celluar immunological function disorder in ITP patients, and decrease of CD4+CD25+ T cell population may be involved in the pathogenesis of ITP.

Objective To evaluate the characteristics of remodeling of Achillis tendon under different training methods, including the functional and structural changes in its insertion, and to extend these experimental findings to improve strategies of prevention of military training injuries. Methods With a special equipment to provoke running and jumping, adult rabbits were trained under different loads and the length of time, then the Achillis tendons were harvested. The tendons and their attachment were examined with light and scanning electron microscopy, as well as analyzed biomechanically. Results The findings showed that the structure of tendon became weak at the fourth week of excessive exercise, as evidenced by changes in micro-structures. Conclusion Excessive load is the major cause of enthesiopathy of the Achillis tendon, and training in cycles may have positive effects in prevention of the pathology.

In August 2001, Chinese military released the "Diagnostic criteria, principles of principles of prophylaxis and treatment of military training injuries" for implementation in the armed forces for the first time in history. The "Criteria" standardizes the definitions of military training injuries, categorizes the various injuries with specific diagnostic criteria, and specifies the principles of their prevention and treatment. This paper gives a brief account of the main contents of the "Criteria", and reiterates the basis of designation of precuresor symptoms of various injuries which occur during military training. The paper points out that the army officers should revise the training plan in regard to its contents, duration and intensity timely, if the additive ratio of limb pain and joint swelling reaches 20% and 10%, respectively, during the 3rd-4th training week. During the period of implementation, the "Criteria" has shown to be practical and easy to operate. It has not only provided standardized technical bases for the case reports, stastistics, clinical investigations, and popularization of technics, but also provided a reliable and comparable theoretical foundation to the officers to formulate training protocols. Furthermore, because of the application of the principles of prevention and treatment of injuries during training as documented in the text, as well as psychological education has been emphasized, the incidence of military training injury has been lowered markedly. Through epidemiological study in 10 different army groups, it has been shown that the yearly add up ratios have been lowered from 30%-45% to below 10%.

Objective To study the adaptability of rat' skeletal muscle to medium load on endurance training. Methods 40 male Wistar rats were used in an upward-slope running with medium load. Then the ultrastructural changes of the soleus muscle and IGF-1 expression in different training stages were observed. Results The pathologic change of muscular tissues suggested that there was kinesic injury in rat soleus muscle, and the injury happened most seriously in the third week, when muscle structure experienced a relative 'fragile' period. At the same time, there was an obvious increase of IGF-1 in muscular tissues, which indicated that the muscle started to repair of itself immediately after injury. The abnormity rate of Z-cure and IGF-1 level began to decline gradually after 4 weeks, meanwhile the pathologic change reduced. Conclusion After been injured, the pathologic changes of muscle underwent a course of going serious then reducing accompanied with the reparation. The fact indicated that rat skeletal muscle showed a good adaptability to the injury accumulation.

Objective To compare the effectiveness of running training, swimming training and intensified cyclic training by means of morphometry and to determine the most effective training mode to develop the aerobic ability of the skeletal muscle. study the adaptability of rat skeletal muscle to different endurance training modes. Methods Twenty-five healthy male Wistar rats of 8 weeks old were divided into five groups, 10 of them having no exercise be divided into control group 1 and control group 2, others took part in running exercise, swimming exercise and intensified cyclic exercise respectively. The rats in control group1 were killed at the beginning of the exercise. The free activity group was taken as the control group 2, the rats were not given any interventions, they could move freely in the cage and be killed at the end of the exercise (n=5). The rats in running group were forced to take part in an upward-slope (+5?) running task of medium load, the rats should kept running at the speed of 16m per minute for 45 minutes. The rats in swimming group were forced to keep swimming in the pool for 45 minutes, The rats in intensified cyclic group were kept running for 30 minutes (the running mode was same to the running group), then had a rest for 15 minutes, and at last the rats were forced to keep swimming in the pool for 30 minutes. The rats in training groups were trained for 6 days every week with 1-day rest for 8 continuous weeks. We observed the change of aerobic ability of the soles muscle in different training modes. Results The three kinds of endurance training developed the aerobic metabolism ability of skeletal muscle in rats effectively. The intensified cyclic training can do better than swimming training and running training in promoting remodeling reconstruction of skeletal muscle, and developing the muscle, function. Conclusion The intensified cyclic training can shorten the period of remodeling reconstruction of skeletal muscle, and more effective in developing the aerobic ability of the skeletal muscle than other endurance training modes.

Objective The purpose of this study is to evaluate the effects of intensified cyclic training on bone moulding reconstruction, and to found the scientific basis of intensific cyclic training as a new training methods. Methods 48 male rats were equally divided into two groups: intensive repetitive training group and control group. The upper segment of tibia was harvested from the animals at the 1st, 2nd, 3rd, 4th, 5th and 6th week after the onset of the experiment for the measurement of cortical thickness (CT), mean wall thickness (MWT) and the percentage of osseous surface of trabeculae and surface area of trabecular Absorption (Sr). The data of intensive repetitive trained group were compared with those of the control group. 1 303 recruits were divided into control group and experimental group. In the experimental group, they were trained by intensive repetitive training program instead of the traditional training program. The incidence of stress fracture was compared between two groups. Results CT (1.37?0.09, 1.86?0.14, 1.94?0.23), MWT (56.37?2.52, 57.28?3.01, 58.23?2.93) and Sos (12.29?1.17, 14.61?1.84, 15.51?1.13) were higher in the training group than control groups at the fourth week, the fifth week and the sixth week, respectively (P