Magnetic nano gene vector is one of the non-viral gene vectors, modified by functional group to bind cationic transfect reagents. Coupling magnetofection with the universal lipofection we developed a novel somatic cell transfection method as the so-called liposomal magnetofection (LMF). This approach is potential to provide somatic cell cloning with stable genetic cell lines to cultivate transgenic animals. In order to construct such liposomal magnetic gene vectors complexes system, we used nano magnetic gene vector to combine with liposomal cationic transfect reagents by molecular self-assembly. This vectors system successfully carried exogenous gene and then transfected animal somatic cells. Here, we conducted atomic force microscopy (AFM), zeta potential-diameter analysis and other characterization experiments to investegate the size distribution and morphology of magnetic nanoparticles, the way of the vectors to load and concentrate DNA molecules. Our data reveal that, the LMF of Pig Kidney cells exhibited higher transfection efficiency comparing with the transfection mediated by the commercial lipofectamine2000. Moreover, LMF method overcomes the constraint of transient expression mediated by lipofection. Meanwhile, MTT assay showed low cytotoxicity of LMF. Hence, LMF is a feasible, low cytotoxic and effective method of cell transfection.
Animals ; Cations ; Cell Line ; DNA ; Genetic Vectors ; Kidney ; cytology ; Liposomes ; Magnetics ; Nanoparticles ; Swine ; Transfection

Objective:To investigate the risk factors and distribution characteristics for skip lateral lymph node metastasis in patients of papillary thyroid carcinoma (PTC).Methods:Clinical data of 720 PTC patients with postoperative pathologically confirmed lymph node metastasis in the lateral cervical region at Xijing Hospital of Air Force Military Medical University from Jan 2014 to Dec 2021 were retrospectively analyzed. According to whether there is skip metastasis in the lymph nodes of the patients, patients were divided into skip metastasis group (92 cases) and non-skip metastasis group (628 cases).Results:The rate of lymph node skip metastasis in PTC in this study was 12.7% (92/720). Women ( OR=1.890, 95% CI:1.118-3.198, P=0.018), age ≥55 years ( OR=2.508, 95% CI:1.412-4.454, P=0.002), tumor involving the upper pole ( OR=1.919, 95% CI:1.220-3.018, P=0.005), and unilateral glandular lobe lesions ( OR=1.926, 95% CI:1.153-3.214, P=0.012) were independent risk factors for lymph node skip metastasis. Skip metastasis is most likely to occur in region Ⅲ, followed by region Ⅵ, Ⅱ and Ⅴ. Larger cancer tended to have more than one jumping metastases in regions Ⅲ and Ⅳ ( P=0.001, 0.016). Conclusion:In female PTC patients, age≥55 years , tumors involving upper pole, and unilateral lesions,are all the hazardous factor for skip metastasis in the lateral neck region.

Objective To establish an artificial intelligence voice follow-up system for differenti-ated thyroid cancer and evaluate the completeness and accuracy of information collection by this sys-tem.Methods Based on the postoperative medication and follow-up status of patients with differenti-ated thyroid cancer,an artificial intelligence voice follow-up system for differentiated thyroid cancer was established.From February 25 to 29,2024,a total of 2,471 differentiated thyroid cancer pa-tients without follow-up in the thyroid cancer database management system of the Department of Thy-roid and Breast Vascular Surgery of the Hospital were followed up by telephone,and 220 patients(approximately 10％of the total)were randomly selected for completeness and accuracy analyses of their call records.Results This study successfully established an artificial intelligence voice follow-up system for differentiated thyroid cancer,and the overall call connection rate was 88.9％.For pa-tients with successful calls,the average follow-up time of the artificial intelligence voice follow-up sys-tem was(1.7±0.5)minutes,the overall information collection completeness rate was 83.8％,with a completeness rate of 92.9％for follow-up at 3 months after surgery and 82.7％for long-term follow-up,and the differences were statistically significant(x2=15.200,P＜0.001).The accuracy rate of information collection by this follow-up system was 94.1％.The accuracy rates of information collec-tion for patients who spoke dialect and Mandarin during follow-up were 92.2％and 95.4％respective-ly,and the difference was not statistically significant(x2=0.957,P=0.389).Conclusion The arti-ficial intelligence voice follow-up system for differentiated thyroid cancer has high efficiency,complete-ness,and accuracy in information collection,which can reduce human costs for follow-up and has good application prospects in large-scale follow-up of populations with differentiated thyroid cancer.

Objective To establish an artificial intelligence voice follow-up system for differenti-ated thyroid cancer and evaluate the completeness and accuracy of information collection by this sys-tem.Methods Based on the postoperative medication and follow-up status of patients with differenti-ated thyroid cancer,an artificial intelligence voice follow-up system for differentiated thyroid cancer was established.From February 25 to 29,2024,a total of 2,471 differentiated thyroid cancer pa-tients without follow-up in the thyroid cancer database management system of the Department of Thy-roid and Breast Vascular Surgery of the Hospital were followed up by telephone,and 220 patients(approximately 10％of the total)were randomly selected for completeness and accuracy analyses of their call records.Results This study successfully established an artificial intelligence voice follow-up system for differentiated thyroid cancer,and the overall call connection rate was 88.9％.For pa-tients with successful calls,the average follow-up time of the artificial intelligence voice follow-up sys-tem was(1.7±0.5)minutes,the overall information collection completeness rate was 83.8％,with a completeness rate of 92.9％for follow-up at 3 months after surgery and 82.7％for long-term follow-up,and the differences were statistically significant(x2=15.200,P＜0.001).The accuracy rate of information collection by this follow-up system was 94.1％.The accuracy rates of information collec-tion for patients who spoke dialect and Mandarin during follow-up were 92.2％and 95.4％respective-ly,and the difference was not statistically significant(x2=0.957,P=0.389).Conclusion The arti-ficial intelligence voice follow-up system for differentiated thyroid cancer has high efficiency,complete-ness,and accuracy in information collection,which can reduce human costs for follow-up and has good application prospects in large-scale follow-up of populations with differentiated thyroid cancer.

Objective:To investigate the clinicopathologic features and prognostic factors of breast cancer patients with tumor deposits in the ipsilateral axillary region.Methods:We retrospectively analyzed the clinicopathologic data and follow-up results of 155 patients with breast cancer diagnosed for the first time and complicated with tumor deposits in the ipsilateral axillary region in the Department of Thyroid-Breast-Vascular Surgery of Xijing Hospital from January 2008 to September 2018. Kaplan-Meier method was used for survival analysis. Log rank test was used for the univariate analysis of prognostic factors, and Cox regression was used for multivariate analysis.Results:The median disease free survival (DFS), median distant metastasis free survival (DMFS), and median overall survival (OS) of the 155 patients were 52.0 months, 66.6 months, and 102.2 months, respectively. The 5-year and 10-year DFS rates were 45.7% and 23.1%, the 5-year and 10-year DMFS rates were 56.9% and 28.9%, and the 5-year and 10-year OS rates were 79.3% and 46.0%, respectively. Multivariate Cox regression analysis showed that family tumor history ( HR=0.362, 95% CI: 0.140-0.937), clinical T stage (T3: HR=3.508, 95% CI: 1.380-8.918; T4: HR=2.220, 95% CI: 1.076-4.580), estrogen/progesterone receptor status ( HR=0.476, 95% CI: 0.261-0.866), number of tumor deposits ( HR=1.965, 95% CI:1.104-3.500) and neoadjuvant chemotherapy ( HR=1.961, 95% CI: 1.032-3.725) were independent influencing factors for DFS. Molecular subtype [human epidermal growth factor receptor-2(HER-2) positive and hormone receptor negative: HR=7.862, 95% CI: 3.189-19.379], number of tumor deposits ( HR=2.155, 95% CI: 1.103-4.212), neoadjuvant chemotherapy ( HR=5.002, 95% CI: 2.300-10.880) and radiotherapy ( HR=2.316, 95% CI: 1.005-5.341) were independent influencing factors of DMFS. Histological grade ( HR=4.362, 95% CI: 1.932-9.849), estrogen/progesterone receptor expression ( HR=0.399, 95% CI: 0.168-0.945), HER-2 expression ( HR=2.535, 95% CI: 1.114-5.768) and neoadjuvant chemotherapy ( HR=4.080, 95% CI: 1.679-9.913) were independent influencing factors of OS. Conclusions:The presence of tumor deposits weakens the influence of axillary lymph node status and distant metastases on the prognosis of breast cancer patients. Therefore, a clinicopathological staging system taking into account tumor deposits should be developed. Since the number of tumor deposits affects the risk of recurrence and metastasis of breast cancer patients, we recommend that the number of tumor deposits should be reported in detail in the pathological report after breast cancer surgery.

Objective:To investigate the clinicopathologic features and prognostic factors of breast cancer patients with tumor deposits in the ipsilateral axillary region.Methods:We retrospectively analyzed the clinicopathologic data and follow-up results of 155 patients with breast cancer diagnosed for the first time and complicated with tumor deposits in the ipsilateral axillary region in the Department of Thyroid-Breast-Vascular Surgery of Xijing Hospital from January 2008 to September 2018. Kaplan-Meier method was used for survival analysis. Log rank test was used for the univariate analysis of prognostic factors, and Cox regression was used for multivariate analysis.Results:The median disease free survival (DFS), median distant metastasis free survival (DMFS), and median overall survival (OS) of the 155 patients were 52.0 months, 66.6 months, and 102.2 months, respectively. The 5-year and 10-year DFS rates were 45.7% and 23.1%, the 5-year and 10-year DMFS rates were 56.9% and 28.9%, and the 5-year and 10-year OS rates were 79.3% and 46.0%, respectively. Multivariate Cox regression analysis showed that family tumor history ( HR=0.362, 95% CI: 0.140-0.937), clinical T stage (T3: HR=3.508, 95% CI: 1.380-8.918; T4: HR=2.220, 95% CI: 1.076-4.580), estrogen/progesterone receptor status ( HR=0.476, 95% CI: 0.261-0.866), number of tumor deposits ( HR=1.965, 95% CI:1.104-3.500) and neoadjuvant chemotherapy ( HR=1.961, 95% CI: 1.032-3.725) were independent influencing factors for DFS. Molecular subtype [human epidermal growth factor receptor-2(HER-2) positive and hormone receptor negative: HR=7.862, 95% CI: 3.189-19.379], number of tumor deposits ( HR=2.155, 95% CI: 1.103-4.212), neoadjuvant chemotherapy ( HR=5.002, 95% CI: 2.300-10.880) and radiotherapy ( HR=2.316, 95% CI: 1.005-5.341) were independent influencing factors of DMFS. Histological grade ( HR=4.362, 95% CI: 1.932-9.849), estrogen/progesterone receptor expression ( HR=0.399, 95% CI: 0.168-0.945), HER-2 expression ( HR=2.535, 95% CI: 1.114-5.768) and neoadjuvant chemotherapy ( HR=4.080, 95% CI: 1.679-9.913) were independent influencing factors of OS. Conclusions:The presence of tumor deposits weakens the influence of axillary lymph node status and distant metastases on the prognosis of breast cancer patients. Therefore, a clinicopathological staging system taking into account tumor deposits should be developed. Since the number of tumor deposits affects the risk of recurrence and metastasis of breast cancer patients, we recommend that the number of tumor deposits should be reported in detail in the pathological report after breast cancer surgery.

[摘 要] 目的：探究甘露糖结合凝集素2（LMAN2）在激素受体（HR）阳性乳腺癌组织中的表达水平与乳腺癌患者预后的关系及其对MCF-7 细胞增殖和迁移的影响。方法：通过TCGA、Bc-GenExMiner、GEPIA和Kaplan-Meier Plotter数据库分析LMAN2在乳腺癌组织和正常乳腺组织中的差异性表达及其与患者预后的关系。采用小RNA干扰技术将si-LMAN2#1、si-LMAN2#2及si-NC转染至MCF-7细胞，将过表达LMAN载体（pc-LMAN）及空载体pcDNA3.1阴性对照（pc-NC）转染至MCF-7细胞，实验分为si-LMAN2#1、si-LMAN2#2、si-NC、pc-LMAN2和pc-NC组。通过qPCR和WB实验检测各组细胞中LMAN2 mRNA和蛋白的表达水平，CCK-8、克隆形成、Transwell迁移、WB等实验检测敲低和过表达LMAN 2对MCF-7细胞增殖、克隆形成、迁移及AKT信号通路相关蛋白表达的影响。结果：LMAN2在乳腺癌组织中的表达水平显著高于正常乳腺组织（P<0.001）。HR阳性乳腺癌组织中LMAN2表达水平显著高于HR阴性乳腺癌组织（P<0.001）；LMAN2高表达与HR阳性乳腺癌患者不良预后有关联。敲低LMAN2可显著降低MCF-7细胞的增殖和迁移能力（P<0.01或P<0.001），过表达LMAN2可显著提高MCF-7细胞的增殖和迁移能力（均P<0.001）。敲低LMAN2组MCF-7细胞中PTEN和P21蛋白表达水平均显著升高，p-AKT蛋白表达水平显著降低（均P<0.01）。结论：LMAN2在乳腺癌组织和HR阳性乳腺癌组织中高表达，且与不良预后有关联。LMAN2高表达与MCF-7细胞增殖和迁移有关联，其作用机制可能涉及AKT信号通路。