Objective:To explore the correlation among serum uric acid (UA) level and severity of coronary heart disease (CHD) ,adverse cardiovascular events .Methods :Clinical data of 403 patients undergoing coronary angiog-raphy in our hospital were retrospectively analyzed .They were divided into CHD group [n=308 ,including 137 cases with stable angina pectoris (SAP) and 171 cases with acute coronary syndrome (ACS)] and normal control group (n=95 ,non-CHD patients) .UA level was compared among all groups and its correlation with CHD severity was fur-ther analyzed .According to UA level ,CHD patients were divided into low UA group (n=147) and high UA group (n=161) ,after 12-month follow-up ,incidence rate of major adverse cardiovascular events (MACE) uring follow-up was compared between two groups .Results:Serum UA level of ACS group was significantly higher than that of nor-mal control group [ (318.44 ± 69.07)μmol/L vs .(295.38 ± 80.08)μmol/L ,P=0.003];along with number of dis-eased coronary vessels increased and CHD severity aggravated (Gensini score rose ) ,serum UA level showed an in-creasing trend [single-branch (316.58 ± 95.27 ) μmol/L vs . double-branch (335.26 ± 43.26 ) μmol/L vs . multi-branch (346.53 ± 86.74) μmol/L ;low score group (312.42 ± 48.26) μmol/L vs .middle score group (346.58 ± 47.36)μmol/L vs .high score group (363.84 ± 54.68)μmol/L , P<0.05 or <0.01] .Pearson correlation analysis indicated that serum UA level was positively correlated with Gensini score (r=0.583 ,P<0.05);MACE incidence rate in high UA group was significantly higher than that of low UA group (58.38% vs .36.24% , P=0.012) .Con-clusion:Serum uric acid level is positively correlated with severity of coronary heart disease ,which may be an inde-pendent risk factor for coronary heart disease .

Objective To analyze the flavonoid content of common fruits consumed in China.Method Thirty-eight fruits were sampled from Beijing,Tianjin,Shanghai and Hangzhou.The contents of quercetin,kaempferol,luteolin,myricetin,apigenin were determined with high-performance liquid chromatography.Results Pomegranate and hawthorn were the highest in contents of 5 flavonoids among 38 fruits.Quercetin distributed more widely and was more abundant than other flavonoids in fruits.The daily intake of 5 flavonoids from fruits by Chinese people was estimated to be 2.80 mg.Conclusion The content of flavonoids varies significantly among different fruits and quercetin is the most common flavonoid contained in fruits.

Objective: To study if the immune-enhancing effect of Arg was mediated via liver.Methods: The direct effect of Arg on T cell proliferation was measured by 3H-TdR incorporation. Rat hepatocytes were primarily cultured in serum-free DMEM/F12 medium, and then cultured in medium containing Arg(?mol/L:0,7.5,75,750,7 500), and the supernatant was collected at 0, 24, 48, 72 hours, and added to splenocytes culture, and T cell proliferation, NK cell activity and IL-2 activity,〔Ca 2+〕i were measured respectively.Results: Arg had no direct effect on splenocyte proliferation. The hepatocyte culture supernatant significantly increased the lymphocyte〔Ca 2+〕i ,IL-2 activity and T lymphocyte proliferation; 7 500 ?mol/L was most effective. Conclusion: Arg may enhance immune function via secretion of bioactive molecules by hepatocytes.

Objective To detect 14 genes including fibrillin-1(FBN1) and so on mutations in 17 patients with Marfan syndrome(MFS) and family members of 2 patients and to investigate the correlation between FBN1 gene mutation and MFS.Methods Genomic DNAs were extracted from whole blood sample of 17 patients and 43 family members.After DNA samples were amplified by polymerase chain raction(PCR), we used capture panels to get target genes which would be sequenced by Illumina HiSeq2500 Analyzers(Illumina, SanDiego, USA).The target genes included ACTA2、CBS、FBN1、FBN2、MYH11、COL3A1、SMAD3、TGFBR1、TGFBR2、MYLK、MSTN、COLA2、TGFB2 and SLC2A10.The results of sequencing would be compared with multiple databases, including NCBI dbSNP, HapMap, 1000 human genome dataset and database of 100 Chinese healthy adults, to find gene mutation.Finally, these mutations would be validated using conventional Sanger sequencing methods.Results A total of 10 FBN1 mutations and 1 actin alpha2(ACTA2) mutation in 17 patients were identified, of which 8 FBN1 mutations and 1 ACTA2 mutation were novel.One FBN1 mutation was underwent family investigation and we found in this family, all patients had this mutation and others did not have it.Conclusion Missense mutation of c.7280G>A in the 59th exon of FBN1 gene is new pathogenic mutation for MFS.The other 8 novel mutations may be the pathogenic factors of MFS.

Objective To analyze the flavonoid content of common vegetables consumed in China.Method Forty-six vegetables were sampled from Beijing,Tianjin,Shanghai and Hangzhou.The contents of quercetin,kaempferol,luteolin,myricetin,apigenin were determined with high-performance liquid chromatography.Results Mei celery sampled from Hangzhou was the highest in content of 5 flavonoids among 46 vegetables.Quercetin distributed more widely and was more abundant than other flavonoids in vegetables.The daily intake of 5 flavonoids from vegetables by Chinese people was estimated to be 13.90 mg,in which quercetin accounted for 51.1%.Conclusion The content of flavonoids varies significantly among different vegetables and quercetin is the most common flavonoid contained in vegetables.The dietary intake of flavonoids was more from vegetables than fruits in Chinese people.

Objective To explore the application value of percutaneous dilational tracheostomy in the neurological intensive patients. Methods The clinical data of 298 neurological intensive patients treated with percutaneous dilational tracheotomy were analyzed retrospectively from June 2010 to July 2016. Results Percutaneous dilational tracheostomy was successfully performed in all of the 298 cases. The mean operation duration was (6.8 ± 2.2) minutes with a rang of 2 to 15 min. There were 1 case with tracheal tube placement difficulties, 1 case in whom the trachea spatium was strayed into, 1 case with arrhythmia, 10 cases with mild bleeding and 5 cases with moderate bleeding. No severe bleeding, subcutaneous emphysema, pneumothorax, tracheoesophageal fistula and other complications caused by the operation were found. And no death case was found. Conclusions Percutaneous dilational tracheostomy is a rapid and minimally invasive technique, which is a better choice for patients with severe neurological diseases.

Objective:To investigate the effects of quercetin on metabolic profiles of portal blood plasma in rats. Method:Twenty five male Wistar rats were administered orally with 40 mg/kg bw quercetin. Portal blood was collected at 0,0.5h,1 h,2 h and 4 h after dosing respectively and analyzed by 1H NMR. The acquired data were subjected to partial least-squares discriminant analysis. Results:The identified biochemical effects associated with quercetin dosing included increased plasma concentrations of dimethyl sulfoxide,lactate,glucose,alanine,glutamate,succinate,?-hydroxybutyrate,acetone and decreased plasma concentrations of citrate and tyrosine. Conclusion:Quercetin may change the intestinal endogenous metabolism significantly in rats.

The effects of high concentrations (1-5mmol/L) of different amino acids in Hanks solution on the proliferation of Ehrlich ascites tumor cells and Walker 256 carcinosarcoma cells were examined. In order to demonstrate the inhibitory effect of some amino acids at high concentration level, the further studies were conducted on Ehrlich ascites tumor cells, human hepatoma cells and lung cancer cells, using Eagle's MEM as culture medium. The possible toxicity of the high concentrations of single amino acids to normal tissue cells was tested on mouse bone marrow cells. Although some amino acids exhibited a significant inhibitory effect, the responses of different tumor cells to the high concentrations of different amino acids were not entirely similar. Moreover, the effects presented by some amino acids on Ehrlich ascites tumor cells in Hanks solution could be diminished or eliminated in Eagle's MEM. Among the aromatic and basic amino acids, only the tryptophan was found to be inhibitory to the multiplication of mouse bone marrow cells at high concentration levels in vitro.