Rheumatoid arthritis belongs to arthralgia syndrome in the theory of traditional Chinese medicine， and cold-dampness obstruction syndrome and dampness-heat obstruction syndrome are core syndromes and main syndrome differentiation types of this disease. Fine therapeutic effects have been obtained in the long-term clinical practice of many famous traditional Chinese medicine practitioners following the syndrome differentiation and treatment based on the guiding principles of cold and heat. To adapt to the clinical diagnosis practice of combining disease differentiation and syndrome differentiation， and to better carry out basic research on integrated Chinese and Western medicine and preclinical study on new traditional Chinese medicines， Guidelines for Establishing Animal Models of Rheumatoid Arthritis with Cold-Dampness Obstruction Syndrome and Dampness-Heat Obstruction Syndrome （hereinafter referred to as the Guidelines） were compiled by our research group， in cooperation with the renowned experts in research fields including traditional Chinese medicine， clinical medicine， zoology and evidence-based medicine， which provide a meaningful reference for scientific research， teaching and clinical applications. The compilation process of the Guidelines was guided by the theory of disease and syndrome integration and the principles of "evidence takes the main place， consensus plays an auxiliary role， and experience serves as the reference". Based on the comprehensive evaluation of pathogenesis homology， behavioral phenotypic consistency， and drug treatment predictability compared between animal models and human diseases， by the nominal group method， "recommendations" were formed for recommendations supported by evidence， and "consensus recommendations" were formed for recommendations not supported by evidence. Guidelines were formed involving content such as animal types， arthritis modeling methods， external stimulation conditions， and modeling assessment indicators during the establishment of the animal models of rheumatoid arthritis with cold-dampness obstruction syndrome and dampness-heat obstruction syndrome. The Guidelines are applicable for the disease and syndrome research on rheumatoid arthritis， investigation of therapeutic mechanisms， and development of new traditional Chinese medicine. The Guidelines also provide a reference for the establishment of guidelines on other types of diseases and syndromes combined with animal models to further promote the modernization of traditional Chinese medicine research and its integration with international academic development.

The Guidelines for Establishing Animal Models of Rheumatoid Arthritis with Cold-dampness Obstruction Syndrome and Dampness-heat Obstruction Syndrome （hereinafter referred to as the Guidelines） （No. T/CACM1567-2024） was published by Chinese Association of Chinese Medicine on January 11， 2024. To assist researchers and medical workers in understanding and applying the Guidelines more accurately， and also to provide reference and assistance for the establishment of guidelines on other types of diseases and syndromes combined with animal models， this paper made a declaration of the workflow， technological links， development references， promotion of its application and after-effect evaluation of the Guidelines that has been made according to the requirements of "Draft Group Standard of the Standardization Office of the Chinese Association of Traditional Chinese Medicine".

Delirium has a high incidence and is associated with a poor patient prognosis.The pathogenesis of delirium is still unclear.Although antipsychotic drugs are the main intervention in clinical practice,there are currently no drugs that can definitively improve the long-term health-related quality of life in patients with delirium.In this study,we review research on the associations between epigenetic modifications such as non-coding RNAs,DNA methylation,and histone acetylation and the development of delirium,with the aim of providing information to support clinical decisions on the early identification and diagnosis of delirium,as well as its treatment and prognosis and the development of relevant targeted drugs.

Delirium has a high incidence and is associated with a poor patient prognosis.The pathogenesis of delirium is still unclear.Although antipsychotic drugs are the main intervention in clinical practice,there are currently no drugs that can definitively improve the long-term health-related quality of life in patients with delirium.In this study,we review research on the associations between epigenetic modifications such as non-coding RNAs,DNA methylation,and histone acetylation and the development of delirium,with the aim of providing information to support clinical decisions on the early identification and diagnosis of delirium,as well as its treatment and prognosis and the development of relevant targeted drugs.

Objective To analyze the causal relationship between brain resting state functional magnetic resonance imaging(rsfMRI)network and myocardial infarction using the Mendelian randomization(MR)research method.Methods Four analytical methods of MR[inverse-variance weighted(IVW),weighted median method(WME),weighted mode method,and MR-Egger method]were used to conduct a two-way two-sample analysis between 191 types of rsfMRI phenotypes of the brain(34 691 types)and myocardial infarction.And based on the aggregated analysis of genome-wide association studies(GWAS)data,MR was used to explain the causal relationship between the two.Then,the IVW method was used for analysis and research as the main result,and other analytical methods were used as supplementary analyses.Finally,the credibility of the results was verified by using sensitivity analysis.Results The neural connection activities located at the edge of the fusiform gyrus of the temporal lobe and the default mode network[odds ratio(OR)=1.260,95%confidence interval(95%CI)1.120-1.420,P<0.001]may increase the risk of myocardial infarction.In addition,the motor and subcortical cerebellar networks located in the posterior central gyrus,anterior central gyrus,and cerebellum(OR=0.770,95%CI was 0.680-0.880,P<0.001),and the subcortical cerebellar and motor networks located in the cerebellum and paracentric lobules(OR=0.810,95%CI was 0.720-0.900,P<0.001)and the neural connection activities of the motor and subcortical cerebellar networks located in the posterior central gyrus,anterior central gyrus and subcortex(OR=0.730,95%CI was 0.630-0.840,P<0.001)were associated with a reduced risk of myocardial infarction.Sensitivity analysis showed that the research results were reliable,and after the reverse MR study,it was confirmed that there was no reverse causal association between the two.Conclusion The network signals located at the edge of the fusiform gyrus of the temporal lobe and in the default mode may be potential risk factors for myocardial infarction;the motor and subcortical cerebellar networks located in the posterior central gyrus,anterior central gyrus and cerebellum,as well as the subcortical cerebellar and motor networks located in the cerebellum and paracentric lobules,and the neural connection activities of the motor and subcortical cerebellar networks located in the posterior central gyrus,anterior central gyrus and subcortex may be potential protective factors,providing new ideas for the prevention and treatment of myocardial infarction.

Healthcare-associated infection(HAI)is a global public health problem that poses a significant socio-economic burden.Hand hygiene is still considered as one of the most effective measures to prevent the spread of pathogens and reduce the incidence of HAI.This paper systematically reviews the latest progress in hand hygiene-related new products,intelligent monitoring technology,and compliance promotion strategies,aiming to provide scientific basis for the management and optimized scheme of hand hygiene in clinical practice in medical institutions in China,ultimately enhance the hand hygiene compliance of healthcare workers,ensure patient safety,and reduce the burden of HAI.

Healthcare-associated infection(HAI)is a global public health problem that poses a significant socio-economic burden.Hand hygiene is still considered as one of the most effective measures to prevent the spread of pathogens and reduce the incidence of HAI.This paper systematically reviews the latest progress in hand hygiene-related new products,intelligent monitoring technology,and compliance promotion strategies,aiming to provide scientific basis for the management and optimized scheme of hand hygiene in clinical practice in medical institutions in China,ultimately enhance the hand hygiene compliance of healthcare workers,ensure patient safety,and reduce the burden of HAI.

Objective To analyze the causal relationship between brain resting state functional magnetic resonance imaging(rsfMRI)network and myocardial infarction using the Mendelian randomization(MR)research method.Methods Four analytical methods of MR[inverse-variance weighted(IVW),weighted median method(WME),weighted mode method,and MR-Egger method]were used to conduct a two-way two-sample analysis between 191 types of rsfMRI phenotypes of the brain(34 691 types)and myocardial infarction.And based on the aggregated analysis of genome-wide association studies(GWAS)data,MR was used to explain the causal relationship between the two.Then,the IVW method was used for analysis and research as the main result,and other analytical methods were used as supplementary analyses.Finally,the credibility of the results was verified by using sensitivity analysis.Results The neural connection activities located at the edge of the fusiform gyrus of the temporal lobe and the default mode network[odds ratio(OR)=1.260,95%confidence interval(95%CI)1.120-1.420,P<0.001]may increase the risk of myocardial infarction.In addition,the motor and subcortical cerebellar networks located in the posterior central gyrus,anterior central gyrus,and cerebellum(OR=0.770,95%CI was 0.680-0.880,P<0.001),and the subcortical cerebellar and motor networks located in the cerebellum and paracentric lobules(OR=0.810,95%CI was 0.720-0.900,P<0.001)and the neural connection activities of the motor and subcortical cerebellar networks located in the posterior central gyrus,anterior central gyrus and subcortex(OR=0.730,95%CI was 0.630-0.840,P<0.001)were associated with a reduced risk of myocardial infarction.Sensitivity analysis showed that the research results were reliable,and after the reverse MR study,it was confirmed that there was no reverse causal association between the two.Conclusion The network signals located at the edge of the fusiform gyrus of the temporal lobe and in the default mode may be potential risk factors for myocardial infarction;the motor and subcortical cerebellar networks located in the posterior central gyrus,anterior central gyrus and cerebellum,as well as the subcortical cerebellar and motor networks located in the cerebellum and paracentric lobules,and the neural connection activities of the motor and subcortical cerebellar networks located in the posterior central gyrus,anterior central gyrus and subcortex may be potential protective factors,providing new ideas for the prevention and treatment of myocardial infarction.

Objective To explore the effect of Internet+cardiac rehabilitation on hypertension patients with coronary heart disease.Methods 80 patients with hypertension and coronary heart disease admitted to our hospital from January 2022 to April 2023 were randomly divided into the combined group(n=40)and the conventional group(n=40).Both groups were given routine cardiac rehabilitation interventions.The combined group combined adoptionof the Internet for continuous interventionson the basis of routine cardiac rehabilitation interventions.Compare the oxygen uptake,blood pressure,compliance,and self-management scores between two groups before intervention and three months after discharge.Results The AT Mets,AT VO2,peak VO2,and expected value compliance ratio in the combined group were all higher than those in the conventional group(P<0.05);The systolic and diastolic blood pressure in the combination group were lower than those in the control group,and the proportion of complete compliance was significantly higher than that in the control group(P<0.05);The self-management scores of the joint group were higher than those of the conventional group(P<0.05).Conclusion The continuous interventions of Internet plus cardiac rehabilitation can control the blood pressure of patients with hypertension and coronary heart disease,and patients'compliance behavior is significantly im-proved,andthus improving patients'self-management ability,which can be used for reference and promotion in clinical practice.

Objective:To explore the correlation between ultrasonic SWE, blood flow quantitative parameters and the expression levels of MYB-associated protein B (MYBL2) and metastasis-associated 1 (MTA1) in breast cancer tissue.Methods:92 patients with breast cancer diagnosed and treated in our hospital from Jan. 2022 to Dec. 2023 were selected as the study objects, all of whom underwent ultrasonic SWE and blood flow imaging examination. Furthermore, the expression levels of MYBL2 and MTA1 in cancer tissues were detected, and the correlation between ultrasonic SWE and blood flow quantitative parameters and the expression levels of MYBL2 and MTA1 was analyzed.Results:The difference between maximum elastic modulus (Emax) and mean elastic modulus (E mean) in breast cancer patients with different lesion diameters and clinical stages was statistically significant (lesion diameters: t=4.561, 7.347; Clinical stage: t=4.218, 7.554, P<0.001) ; There were statistically significant differences in maximum blood flow velocity (V max), resistance index (RI), pulse index (PI) among breast cancer patients with different clinical stages, differentiation degrees and lymph node metastasis (clinical stage: t=7.055, 3.774, 3.877; Differentiation degree: F=26.830, 21.052, 20.412; Lymph node metastasis: t=7.425, 3.685, 4.956, P<0.001). There were statistically significant differences in E max, E mean, V max, RI and PI among breast cancer patients with different expression levels of MYBL2 and MTA1 (MYBL2: t=4.042, 10.672, 7.471, 6.137, 5.777; MTA1: t=5.968, 10.470, 8.098, 10.238, 7.565, P<0.001). By Spearman correlation analysis, E max and E mean were positively correlated with lesion diameter, clinical stage, MYBL2 expression level and MTA1 expression level ( P<0.001) ; V max, RI and PI were positively correlated with clinical stage, lymph node metastasis, MYBL2 expression level and MTA1 expression level, and negatively correlated with differentiation degree ( P<0.001) . Conclusion:The expression levels of MYBL2 and MTA1 in breast cancer tissue are correlated with the ultrasonic shear wave elastography and blood flow quantitative parameters, which can be used as an important reference index for clinical diagnosis and treatment of breast cancer.