Objective:To investigate and analysis the distribution situations of Rh blood group system from voluntary blood donors in Foshan area.Methods:Anti-D,anti-C,anti-c,anti-E and anti-e reagents were used to identify Rh blood group system and the RhD(-) was confirmed.The phenotypes,haplotypes and corresponding genotypes frequencies were calculated and the datas were analyzed based on Hardy-Weinberg law.Results:The characteristics of phenotype frequencies in the voluntary blood donors in Foshan area was CCDee＞CcDEe＞CcDee＞ccDEE＞ccDEe＞CCDEe＞CcDEE＞ccdee＞ccDee,Ccdee＞CCdee,ccdEe＞CCDEE,CCdEE,CCdEe,CcdEE,CcdEe and ccdEE.The characteristics of haplotypes frequencies was CDe＞cDE＞cDe＞CDE,de＞Cde＞cdE＞CdE.The characteristics of genes frequencies was D＞d,C＞c,e＞E.The frequency of RhD(-)phenotype was 0.379%.The gene frequency of d was 0.061.The observed value and desired value of the haplotypes and corresponding genotypes were not statistically significant(P>0.05).The data were analyzed well based on Hardy-Weinberg law.Conclusion:The result is reliable according to Hardy-Weinberg law.The distribution of Rh blood group system from voluntary blood donors in Foshan area has geographic and general characteristics.The result is important in guiding the recruitment of voluntary blood donors and banking blood reasonably and enhancing the abilitiy of offering blood for the recipients of rare blood group.

With a PII-300 computer, a microcomputer system for SCCA marker detection is developed in this paper, which is based on Windows operating system and Visual Basic 6.0. More than 1,000 cases have proved that the results from the system completely accord with the one from artificial detection. With this system, such disadvantages are conquered as high labor intensity, low working efficiency and high error rate, and a quantitative detection method for SCCA marker comes into existence.

Objective To establish a method for isolation, purification of bone marrow mesenchymal stem cells (MSCs) and for their differentiation into vascular endothelial cells, and to observe the regulatory role of microRNA126 expression during the differentiation. Methods MSCs were isolated from normal rat bone marrow using gradientdensity centrifugation and repeated attachment method. CD34, CD105and CD73 expressions in MSCs were detected with immunofluorescent staining. MSCs were cultured with MEF medium for differentiating into endothelial cells; CD34, VE-cadherin and microRNA126 expressions were examined by qRT-PCR at different time points. Results Immunofluorescent detection demonstrated that MSCs have been isolated and purified successfully, with MSCs negative for CD34, strongly positive for CD105 and positive for CD73. The purified MSCs had a good uniformity and a purity above 90%. qRT-PCR examination revealed that CD34 and VE- cadherin expressions were not detected on day 1-4 of induction, strongly positive on day 5-6, and on day 7-9, VE-cadherin was still positive, CD34 decreased on day 7, and increased again on day 8-9. Interestingly, the expression of CD34 and microRNA126 mRNA was consistent during the differentiation. Conclusion We have successfully established a method for MSCs isolation and differentiation into endothelial cells; microRNA126 may play a regulatory role in MSCs differentiation into endothelial cells.

Objective To study the changes of pleth variability index (PVI) in patients receiving laparoscopic cholecystectomy under general anesthesia, and to investigate the clinical influencing factors of pre-operation baseline PVI value. Methods Totally 67 patients undergoing laparoscopic cholecystectomy with ASA?-II, aged 18 to 65 years old, were included in the present study. The heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), pulse oximetry (SpO2), perfusion index (PI), and PVI value were monitored with a Masimo pulse oxygen monitor (Radical-7, USA) after entering the operation room. Fluid expansion with 10 mL/kg of balanced solution was given before the induction. Anesthesia was maintained under total intravenous anesthesia (TIVA) with propofol and remifentanil. Results The average baseline PVI value was (16.8±6.3)% in the patients; then it decreased to (10.3±5.4)% after induction of anesthesia and increased to (21.2±9.5)% after establishment of pneumoperitoneum. The pre-operation baseline PVI value (\[19.2±6.5\] %) of patients <45 years old was significantly higher than that in patients ≥45 years old (\[15.5±5.9\]%, P<0.05%). Male patients had a significantly higher PVI (\[18.8±7.5\]%) value than female patients (\[15.3±4.8\]%, P<0.05). Body mass index (BMI) was not significantly associated with pre-operation baseline PVI. Conclusion The baseline value of PVI is higher than 14%, suggesting insufficient volume load in most of patients. Age and sex are both associated with pre-operation PVI value: male patients and those <45 years old have a higher pre-operation baseline PVI value.

Objective To investigate the mutation of low-density lipoprotein receptor (LDL-R) gene in 3 patients with familial hypercholesterolemia (FH) in two generations, so as to discuss the pathogenesis of FH. Methods A 17-year-old patients was selected to undergo physical examination, lipid level test, electrocardiography, cardiac ultrasound and coronary artery angiography. Pedigree analysis was carried out based on family investigation. The promoter and the 18 exons of the LDL-R gene and the flank sequence were amplified by PCR; DNA sequencing was used to detect point mutation. Ninety normal subjects from the native place of the proband and 190 subjects from random population were taken as controls. Results Totally 28 members of 4 generations were examined. The proband, her elder sister and grandaunt had FH with xanthoma, and their total cholesterol (TC) levels were 18. 89 mmol/L, 15. 23 mmol/L, and 12. 89 mmol/L, respectively. Pedigree analysis showed that the genetic pattern of this family was consistent to autosomal dominant inheritance trait. DNA sequencing demonstrated that a G1448A substitution caused a nonsense mutation TGG to TAG in exon 10 of LDL-R gene, a Trp→462 stop mutation. The mutation of the proband, her older sister and grandaunt were homozygous, heterozygous and heterozygous, respectively. The same mutation was not detected in the family members from the proband' s father and people from control group. Conclusion The proband, her elder sister and grandaunt have the same mutation, the Trp→462 stop mutation in exon 10 of LDL-R gene, which might be the key mutation that causes FH in this pedigree.

In allusion to high labor intensity, low working efficiency and high error rate in hospital laboratory, a detection system of the enzyme-linked analyzer based on Multiscan EX instrument is developed. Being easy to operate, the detection system proves effective in enhancing working efficiency. It deserves popularizing because of its comprehensive applications to the detection.

With a PII-300 computer, an intelligentized microcomputer system for HBV marker detection and reporting is developed in this paper, which is based on Windows operating system and accomplished through Visual Basic6.0. More than 10, 000 cases have proved that the result from the system accords with the one from artificial detection completely. This system solves the problems in hospital laboratory such as high labor intensity, low working efficiency and high error rate, and thus a quantitative detection method for HBV marker comes into existence.

Objective: To explore the effectiveness of keystone flap in repairing skin and soft tissue defects around joint. Methods: Between March 2013 and December 2017, 10 patients of skin and soft tissue defects around the joint were repaired with keystone flaps. There were 6 males and 4 females. The age ranged from 17 to 72 years, with an average age of 57.5 years. The skin and soft tissue defects located around the joints, including 2 cases of hip joints, 4 cases of knee joints, 3 cases of elbow joints, and 1 case of wrist joint. The cause of skin and soft tissue defects included scar contracture in 3 cases, basal cell carcinoma in 3 cases, squamous-cell carcinoma in 3 cases, and dermatofibrosarcoma protuberant in 1 case. The area of defects ranged from 4 cm×3 cm to 11 cm×11 cm. The keystone flap was designed beside the wound, and the V-shaped flap was designed at the longer side of the fan-shaped flap. The area of flap ranged from 7 cm×3 cm to 35 cm×10 cm. The flap was constantly compressed by vacuum assisted closure after operation for 3-6 days. Results: Primary wound healing was achieved and the flaps survived. All patients were followed up 6-24 months (mean, 15 months). The texture and color of flap was similar to the adjacent area. The appearance and function of joint were satisfactory. Conclusion: The keystone flap is a feasible and efficient way to repair the wound around joint. Furthermore, the skin color and texture is similar to the surrounding tissue after healing.

Objective To explore the influence of alpha-fetoprotein (AFP) on the expression of drug-resistance gene MDR1 and chemotherapeutic sensitivity in hepatocellular carcinoma (HCC) cells.Methods A HCC cell line SMMC-7721/AFP, which was stably transfected with AFP gene, was established.mRNA and protein expressions of AFP and MDR1 were detected by real-time PCR and Western Blot,respectively.The sensitivity of SMMC-7721/AFP and SMMC-7721/EGFP cells with or without MDR1 silencing by siRNA to doxorubicin was tested by MTT assay.Immunohistochemistry was used to detect the expression of MDR1 genes-coded protein Pgp in 60 cases of HCC tissues, and the relationship between Pgp expression and serum AFP levels was analyzed.Results AFP mRNA and protein could be detected in SMMC-7721/AFP cells, but not in control cells, indicating that the AFP stably transfected cell line was successfully established.MDR1 mRNA and protein levels were higher in SMMC-7721/AFP cells than those in SMMC-7721/EGFP cells.MDR1 mRNA level in SMMC-7721/AFP cells was (52.7 ± 1.5) times as high as that in SMMC-7721/EGFP cells (P ＜ 0.05).The resistance to doxorubicin was increased by (12.8 ± 1.1) times after AFP transfection (P ＜ 0.05).The chemosensitivity to doxorubicin was increased after the expression of MDR1 was knocked down by siRNA.The expression of Pgp in HCC tissues was positively correlated with the serum AFP levels.Conclusion AFP could induce drug-resistance to doxorubicin in HCC cells by increasing the expression of MDR1.

With the development of optics and mechanics, endoscopic technologies are not only used as a simple diagnostic method, but also applied in therapy. Since the beginning of the 21st century, minimally invasive medicine has become the development trend. Many novel technologies have appeared, such as endoscopic mucosal resection, endoscopic submucosal dissection, and peroral endoscopic myotomy. Esophageal motility disorders are common diseases in clinic, which seriously affect the quality of life. Although esophageal manometry is the golden diagnostic standard, endoscopy also plays an important role in diagnosis. There are many therapeutic methods in achalasia and gastroesophageal reflux diseases with good efficacy, while the therapy in other esophageal motility disorders remains in dispute. According to the domestic and foreign research progressions, we introduce the role of endoscopy in the diagnosis and management of esophageal motility disorders.
Endoscopy, Digestive System ; Esophageal Motility Disorders ; diagnosis ; surgery ; Humans