Two hundred and five patients with a confirmed diagnosis of aldosterone-producing adenomas underwent retroperitoneal laparoscopic operation from January 1999 to December 2009 in our hospital. All cases had hypokalemia and hypertension preoperatively. Of all the cases, 80 received total adrenalectomy and 125 received partial adrenalectomy. Operations were successfully performed in all cases. The time of operation ranged from 15 to 150 min [mean(45 ±13) min] and the volume of bleeding during operation ranged from 10 to 200 ml [mean(25 ±10) ml]. The length of hospital stay ranged from 4 to 9 d [mean(6. 8 ± 1. 2) d]. All patients were followed up for 0. 5 - 2 years (mean 1. 2 years). After operations blood potassium levels returned to normal in all cases, blood pressure returned to normal in 168 cases (82.0% ) . Results indicated that partial or total adrenalectomy with retroperitoneal laparoscopy for aldosterone-producing adenomas was safe and feasible technique.

Objective To study on wavelet transform in calcium sparks image de-noising when excitation contraction cou-pling(ECC) was studied and researched by scanning confocal microscopy,in order to elucidate the mechanism of ECC.But due to the noise exist in most calcium sparks image,it is hardly to recognize and analyze the useful information of calcium sparks.So how to increase the signal-to-noise rate is very important in calcium spark studying.Methods By using the self-developed software based on multi-scale wavelet transform to remove the noise exists in the calcium sparks images.Results The experiments shows that the method can be increased the signal-to-noise rate in about 80 calcium sparks images.Con-clusion The new method for calcium spark expression and spatial and temporal analysis is provided.

In modern society,the spectrum of human diseases has changed significandy. Cerebrovascular diseases have become a huge threat to human health. Vascular smooth muscle cells,which constitute the main components of the cerebrovascular wall, play a key role in cerebrovascular diseases. Understanding the physiological metabolism of vascular smooth muscle cells is helpful to the research of cerebrovascular diseases. In recent years, researchers found that Wnt protein and Wnt signaling pathway were widely involved in cerebrovascular diseases. Wnt signaling pathway plays an important role in the proliferation,migration and apoptosis of smooth muscle cells. This review, based on Wnt signaling pathway, will explore the relationship between vascular smooth muscle cells and cerebrovascular lesions.

Objective To indentify the high risk factors of bone metastasis in Chinese patients at the initial stage of prostate cancer (PCa) diagnosis, so as to elucidate the characteristics of patients with very low risk of bone metastasis at the initial stage of prostate cancer. Methods A consecutive series of 496 patients with newly diagnosed PCa between 2010 and 2015 were enrolled in the present study. All the patients were subjected to ECT scan for presence of bone metastasis (BM) using total-body 99mTc MDP scintigraphy regardless of baseline PCa characteristics. Factors including the age of diagnosis, prostate specific antigen (PSA) level at diagnosis, Gleason score, clinical T stage, bone scan and CT/MRI results were analyzed. Univariate and multivariate logistic regression analyses were performed to identify the predictors of bone metastases. Results Of the 496 patients, 81 patients (16.3%) had bone metastases. The PSA levels of patients with BM were significantly higher than those without BM (P<0.001). The mean age of the patients with BM was not significantly older than that of the patients without BM. Patients with higher PSA level, clinical T stage or Gleason score showed a significantly higher risk of BM (P<0.001). Univariate logistic regression analysis showed that PSA>20 ng/mL at diagnosis, clinical stage at T3-T4 and Gleason score≥8 were the risk factors of bone metastasis in PCa patients. The multivariate analysis showed that the PSA level>50 ng/mL and the Gleason score≥8 were the independent predictors of bone metastases. No bone metastasis was found in 79 patients with PSA≤20 ng/mL and at the same time with Gleason score≤6. Conclusion The bone metastases rate is very low in Chinese patients with a PSA level ≤20 ng/mL and at the same time with Gleason score≤6, so a bone scan is not necessary as a routine for these patients with newly diagnosed prostate cancer.

OBJECTIVETo explore the effects and possible mechanisms of decitabine on Molt4 in vitro.

METHODSEffects of decitabine on cells proliferation were detected by using CCK-8, the apoptosis by Annexin V-FITC, cell cycles by propidium iodide-FACS. Discrepancy genes were screened by RNA-seq technique. The CpG methylation of lactoferrin (LTF) gene in Molt4 cells were identified by Bisulfite sequencing PCR (BSP). The expression of LTF mRNA in Molt4 by RT-PCR and LTF protein expression were analyzed by Western blot.

RESULTSDecitabine effectively inhibited proliferation and induced apoptosis for Molt4 cells by an time- and dose-dependent manners. Cell cycles were arrested at the G₀/G₁ phase. The promoter methylation degree of LTF gene in Molt4 cells was 72.3% before decitabine treatment and decreased to 45.0% after treatment with 0.50 μmol/L decitabine for 72 h. After the reduction of methylation, expression of its mRNA and protein increased, meanwhile caspase 3 and caspase 9 protein expression levels increased.

CONCLUSIONThe demethylating drug decitabine can induce apoptosis, detain cell cycle at phase G₀/G₁, inhibit proliferation and up-regulate LTF gene expression in Molt4 cells. LTF may become a new target for acute T lymphoblastic leukemia.

Antimetabolites, Antineoplastic ; Apoptosis ; Azacitidine ; analogs & derivatives ; Caspase 3 ; Cell Cycle ; Cell Line, Tumor ; Cell Proliferation ; DNA Methylation ; Humans ; Lactoferrin ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ; Promoter Regions, Genetic

Objective To investigate the efficacy of dual plating internal fixation with olecranon osteotomy approach for the emergency surgery of complex distal humeral fractures (intereondylar and supracondylar fracture).Methods Forty-seven cases of complex distal humeral fractures were treated with emergency surgery from May 2002 to May 2007.The dual plating internal fLxation was implemented with olecranon osteotomy approach in a posteubital slight arc shaped median incision.Early postoperative rehabilitation was necessary.Results The follow-up period differed from 5 to 50 months,averaged 18 months.All fractures healed in 3-6 months after surgery.The Cassebaum scoring system was used for the assessment of cubital joint function,36 cases were excellent,4 cases were good,5 cases were common,and 2 cases were bad.The excellent and good rate was 85.1%.Conclusions For complex distal humeral fractures,dual plating internal fixation emergency surgery with olecranon osteotomy approach ensures the early postoperative rehabilitation of the cubital joint and the maximum possible functional recovery.It shows the efficacy is satisfactory.

Fifty nine patients with premature ejaculation (PE) received sertraline treatment for 8 weeks.The average intravaginal ejaculation latency time (IELT) and the scores of Chinese index of premature ejaculation (CIPE) were evaluated and the fasting serum leptin levels were measured before and after treatment,all the values were compared with those of 64 normal male controls.The serum leptin levels were higher,the IELT and CIPE scores were lower in PE patients than those in normal controls before treatment.After 8 weeks of treatment with sertraline,the levels of serum leptin in PE patients decreased to normal levels,CIPE and IELT increased.The results indicate that serum leptin can be used as a bioindicator for diagnosis of premature ejaculation and assessment of therapeutic efficacy in PE patients.

A total of 71 patients with premature ejaculation (PE) received hydrochloride sertraline treatment for 8 weeks and their serum levels of leptin and 5-hydroxytrytamine (5-HT) were determined.Their ejaculation functions were assessed with intravaginal ejaculation latency time (IELT) and Chinese index of premature ejaculation (CIPE-5) value.The study was compared with 64 normal males as control.The baseline level of leptin in the trial group was obviously higher than the controls (P ＜ 0.05) while the level of 5-HT in the trial group far lower than the controls (P ＜ 0.05).At 8 weeks,the level of leptin dropped while the level of 5-HT rose in the trial group after treatment.Therefore a high serum level of leptin and a low serum level of 5-HT may become biological indicators for PE.

Objective To investigate the interrelationship between leptin and 5-hydroxy tryptamine (5-HT)and their significance in diagnosis of premature ejaculation(PE).Methods 71 cases with lifelong PE(the trial group)and 64 healthy males(the control group)were enrolled in this case-control study.Baseline recording of intravaginal ejaculation latency time(IELT) using a stop watch and Chinese Index of Premature Ejaculation-5 (CIPE-5) were done.The serum leptin and 5-HT levels were determined.Then the trial group were given sertraline hydrochloride for 8 weeks,while the control group were not given any medicine treatment in this period.Reassessment was done after 8 weeks.Results At the baseline,the leptin level in the trial group was obviously higher than the control group(P ＜ 0.05),while 5-HT level in the trial group was far lower than the control group(P ＜0.05).After 8 weeks,the leptin level of the trial group dropped and the difference had no statistical significance with that of the control group(P ＞0.05).5-HT level of the trial group rose to the level very close to that of the control group after the treatment.Conclusion The high level of serum leptin and low level of serum 5-HT were the meaningful biological indicators for PE,which can potentially be applied in PE diagnosis.

Cerebrovascular diseases such as intracranial atherosclerosis, intracranial aneurysm and ischemic stroke have become the important causes of death and disability. The structural and functional changes of cerebrovascular, which are caused by various pathogenic factors, are the common pathophysiological basis of cerebrovascular diseases. At present, the etiology of cerebrovascular diseases has not been clarified. Growing evidence suggests that matrix metalloproteinases play an important role in the occurrence and development of cerebrovascular diseases. Blocking or inhibiting the expression of matrix metalloproteinases provides a new theoretical basis for non-invasive treatment of cerebrovascular diseases. This review discusses the relationship between matrix metalloproteinases and cerebrovascular diseases.