Objective To implement XML-based electronic medical records' searching and querying using new technology of XML storing and indexing.Methods The information in electronic records was expressed with CDA XML standard,and XML Query and XML IR were used for querying of electronic records.Results Based on XML querying and indexing models,a new electronic medical record XML querying and searching system was built.Conclusion Querying and searching over XML database has been paid more attention to,with wide use of data adapting to HL7 CDA XML standard in electronic medical records.

AIM: To establish HPLC for the determination of icariin and adenosine in Cordyceps Vigorine Tablet. METHODS:MetaChem Polaris ODS C18-A column(4.6 mm × 250 mm, 5 μm) was adopted; acetonitrile-water was the mobile phase; detection wavelength was set at 272 nm for icariin and at 260 nm for adenosine; column linearity within the range of 0. 374-2. 246 μg for icariin and 0. 348-1. 392 μg for adenosine, respectively. The average recovery and RSD were found to be (99.4 ± 1.84) % for icariin ( n = 6), (99.63 ± 1.29 ) % for adenosine,respectively. CONCLUSION: The method is convenient, fast, reliable to operate and suitable for the quality control of Cordyceps Vigorine Tablet.

Objective To study the clinical characteristics and differentiation of stroke mimics during super early intravenous thrombolysis of acute ischemic stroke. Methods The clinical data of patients who received intravenous thrombolysis between Sep. 2013 and Feb. 2015 in Changhai Hospital were retrospectively analyzed. And those with stroke mimics were identified and were compared with those with strokes; the clinical symptoms and laboratory findings were compared between the two groups. Results A total of 212 patients received intravenous thrombolysis, and 7(3. 3%) of them were identified as having stroke mimics. There were no notable differences in the baseline characteristics between mimics and stroke groups. Psychiatric and dementia history were of great value for differentiation of stroke mimics from strokes. MRI, vascular assessment, electroencephalogram (EEG), blood and cerebrospinal fluid assay greatly contributed to the final diagnosis of stroke mimics. Conclusion Only few patients receiving intravenous thrombolysis have been confirmed to have stroke mimics. Medical history combined with MRI may be of great value for differentiation of mimics and strokes.

Objective: To investigate the value of transglutaminase 4 in diagnosis and prognosis prediction of prostate cancer. Methods: Transglutaminase 4 immunostaining was performed with paraffin sections from 159 prostate cancer patients receiving radical prostatectomy, and the staining results were divided into 4 levels: negative staining, weak staining, moderate staining, and strong staining. The clinical and pathological information of the patients were obtained by reviewing the medical records. The follow-up data were collected by reviewing medical records, prostate cancer database of our department, and telephoning the patients or their family members. Expression of transglutaminase 4 and clinical, prognosis data of patients were subjected to statistical analysis. Results: The expression of transglutaminase 4 in the prostatic cancer tissue was significantly higher than that in the adjacent normal tissue (P<0.001); and the expressions were significantly different in patients with different Gleason grades (P<0.001) and different prostate specific antigen levels(P=0.005). Univariate Cox regression analysis indicated that high transglutaminase 4 expression was a risk factor of biochemical recurrence of prostatic cancer (P=0.020), but multivariate Cox regression analysis did not support this finding (P=0.139). Conclusion: Transglutaminase 4 expression is increased in prostate cancer tissues, and the expression is stronger in malignant tissues with higher Gleason grade and prostate specific antigen level.

Objective To explore the value of solifenacin in managing bladder irritative symptoms after transurethral resection of bladder tumor. Methods A total of 116 patients with the diagnosis of non-muscle invasive bladder carcinoma were enrolled in the present study, and they were randomly allocated into experiment group and control group. All patients received transurethral resection of bladder cancer and immediate postoperative instillation of epirubicin. Patients in the experiment group also received solifenacin 5 mg at 6 h before surgery and once a day after surgery for 3 d, while the patients in the control group received placebo. The incidences and severity of catheter related bladder discomfort were recorded at 6, 12, 24, 48 and 72 h after surgery. Results The incidence rate of bladder irritative symptoms significantly decreased from 93.1% in the control group to 67.2% in the experiment group(P=0.001). Compared with the control group, the severity of bladder irritative symptoms in the experiment group was significantly improved at all time points (P<0.05 or P<0.01). Conclusion Solifenacin is effective for managing bladder irritative symptoms after transurethral resection of bladder tumors.

Objective To discuss the method of calling MATLAB in VB application program based on ActiveX.Methods MATLAB provided automatic service support to ActiveX,and MATLAB could be called as ActiveX of interpreter through ActiveX automatic interface.Results Good effect was achieved when this method was applied to noise filtering of patch clamp.Conclusion By using this method,users can embedded mix programming in self-developed application software conveniently,thus reducing the difficulties in programming.

Objective To study on wavelet transform in calcium sparks image de-noising when excitation contraction cou-pling(ECC) was studied and researched by scanning confocal microscopy,in order to elucidate the mechanism of ECC.But due to the noise exist in most calcium sparks image,it is hardly to recognize and analyze the useful information of calcium sparks.So how to increase the signal-to-noise rate is very important in calcium spark studying.Methods By using the self-developed software based on multi-scale wavelet transform to remove the noise exists in the calcium sparks images.Results The experiments shows that the method can be increased the signal-to-noise rate in about 80 calcium sparks images.Con-clusion The new method for calcium spark expression and spatial and temporal analysis is provided.

The nonlinear wavelet method is used to detect ECG signal. The ECG signal disrupted by high-frequency noise is decomposed with a dyadic Daubechies wavelet according to Mallat algorithm. The principle of the nonlinear wavelet detection method is discussed. Along with the Lipschitz criterion, it is applied to denoising the disrupted ECG signal. The ECG signal is effectively recovered while denoised.

In allusion to high labor intensity, low working efficiency and high error rate in hospital laboratory, a detection system of the enzyme-linked analyzer based on Multiscan EX instrument is developed. Being easy to operate, the detection system proves effective in enhancing working efficiency. It deserves popularizing because of its comprehensive applications to the detection.

With a PII-300 computer, an intelligentized microcomputer system for HBV marker detection and reporting is developed in this paper, which is based on Windows operating system and accomplished through Visual Basic6.0. More than 10, 000 cases have proved that the result from the system accords with the one from artificial detection completely. This system solves the problems in hospital laboratory such as high labor intensity, low working efficiency and high error rate, and thus a quantitative detection method for HBV marker comes into existence.