Objective To study the relationship between pulmonary quantitative indicators on 64-slice spiral CT and lung function,and the diagnosis value of breathing bipolar lung CT scan to emphysema.Methods 54 chronic obstructive pulmonary disease patients were divided into the mild group ( n =5 ) , moderate group ( n =21 ) , severe group(n=19) and gravely severe group(n=9).Four groups received breathing bipolar total lung high-resolution CT scan to test lung density at the end of deep inspiration and expiration,breathing bipolar lung density difference and lung volume,and the relationship between lung density/volume and lung function indicators were analyzed.Results There were significant differences in lung density at the end of deep inspiration and expiration(t=13.67,9.89,all P<0.05),the forced expiratory volume in one second(FEV1),forced vital capacity(FVC)and FEV1/FVC among these fourgroups(t=1.15,0.89,7.89,13.76,16.67,all P<0.05).The lung volume at the end of inspiration and expiration increased with the severity of disease,while there were no significant differences among these four groups (t=15.46,13.28,11.47,all P>0.05).The lung volumes at the end of deep inspiration and expiration were obvi-ously correlated with FVC,FEV1 and FEV1/FVC(r=-0.071,-0.083,r=-0.713,-0.542,r=-0.622,0.639, all P<0.05) .The lung volume at the end of deep expiration was obviously correlated with the expected ratio of resid-ual volume to maximum total lung capacity(r=0.622,P<0.05).The deep aspiratory phase lung density was closely correlated with FEV1/FVC and residual volume(r=0.539,P<0.05).Conclusion Pulmonary quantitative indica-tors on 64-slice spiral CT and pulmonary function has good correlation,which can be used to evaluate the severity of emphysema in patients with chronic obstructive pulmonary disease.

Objective To study the window technique in evaluating the chronic obstructive emphysema and pulmonary residual volume in long-term smoker without symptoms.Methods 50 cases with chronic obstructive emphysema proved clinically,50 long-term smokers were undergone spiral CT scan of chest and compared with 30 health volunteer,the images were designed using the mean CT value in smoking group as window level and two times of ?2 as window width.Results The white imaging was appeared in the most of the group of volunteer,the images were dark on different degree in group one,while the images were as ground glass on different degree in group two.Conclusion This window technique is helpful in evaluating the chronic obstructive emphysema and residual volume of lung in long-term smoker

To study the role of a novel MDR-related gene MGr1-Ag in the multidrug resistant (MDR) gastric cancer. The whole length MGr1-Ag gene was cloned, and eukaryotic vectors carrying the full length of MGr1-Ag cDNA and its antisense expression vector were constructed. The sense vector and anti-sense were then transfected into MGC803 cells and SGC7901/VCR cells respectively by lipofectamine. The result showed that a 1.0kb fragment was successfully amplified by RT-PCR and cloned into pUCm-T vector. DNA sequencing suggested that the fragment was the properly encoded MGr1-Ag gene. Recombinant eukaryotic plasmids harboring MGr1-Ag and its antisense expression vector were also obtained by subcloning the gene into pCDNA3.1/V5-His, which was confirmed by endonuclease digestion. As confirmed by Western blot, stable cell strains with up and down-regulated MGr1-Ag expression were constructed. Those cell strains provided the basis for further study on the MGr1-Ag in MDR of gastric cancer.

ObjectiveTo investigate the role of productive factors in the development of primary biliary cholangitis (PBC). MethodsA total of 273 female patients with a definite diagnosis of PBC who visited Xijing Hospital from October 2013 to August 2015 were enrolled. The patients with autoimmune hepatitis, primary sclerosing cholangitis overlap syndrome, and hepatic encephalopathy and those with incomplete data after telephone follow-up were excluded, and 54 female PBC patients who had female relatives were finally enrolled (PBC group). The female relatives who were less than 10 years older or younger than the patients were collected, and those with severe systemic diseases and incomplete data after telephone follow-up were excluded; finally 88 relatives were enrolled (relative group). The questionnaire for female reproductive factors in PBC was used to survey all these enrolled patients and collect data. The t-test was used for comparison of continuous data between groups, the chi-square test was used for comparison of categorical data between groups, and a multivariate logistic regression analysis was used for the analysis of dose-response relationship. ResultsThe PBC group had a significantly higher number of births than the relative group (2.55±1.84 vs 1.84±0.95, t=2.708, P=0.009). Furthermore, there was a significant dose-response pattern between the number of births and the development of PBC (P=0.002). ConclusionThe number of births may be associated with the development of PBC in a dose-response manner. As for the female population susceptible to PBC, a reduction in the number of births may reduce the possibility of PBC.

Objective:To investigate the relationship between common functional gastrointestinal diseases symptoms with psychological factors, diet and lifestyles by using the network analysis method which has achieved great success in the field of psychology in recent years.Method:A questionnaire survey was conducted in two military units using the cluster sampling method during July 2020, and a total of 1 805 subjects were included. Functional gastrointestinal disease symptoms were evaluated with the Gastrointestinal Symptom Rating Scale (GSRS). The state, trait anxiety scale and stress response scale were used to evaluate the mental and psychological state by self-evaluation. R was used to build the network and calculate statistical parameters.Results:1 486 of the 1 805 subjects (82.3%) had experienced functional gastrointestinal diseases symptoms within 2 weeks, but most of them were mild. Network analysis shows that there was a strong interaction between digestive system symptoms with different clinical manifestations (Spearman coefficient ranges 0.31-0.56). There was a clear relationship between functional gastrointestinal symptoms and mental and psychological factors (Spearman coefficient ranges 0.16-0.27), but there was no clear interaction with diet, age, education level, body mass index, etc. Functional gastrointestinal diseases symptoms were connected with mental and psychological factors through two nodes: stress and indigestion. The stability coefficient of node strength correlation was 0.75, indicating that the network was stable.Conclusions:The current study revealed the network structure and features of functional gastrointestinal diseases symptoms with mental and psychological factors. The key linking nodes provided potential interfering target for controlling functional gastrointestinal symptoms related to mental and psychological factors.