1.The clinical significance of peripheral blood IL-33 and Da-jvGlu in acute cerebral infarction patients giving Shenqi injection
Guibo LI ; Rensheng ZHANG ; Jingyao LIU
Journal of Apoplexy and Nervous Diseases 2021;38(4):319-321
Objective To explore the levels of peripheral blood IL-33 and the difference of arteriovenous fistula glucose (Da-jvGlu) after giving Shenqi glucose injection in acute cerebral infarction patients.Methods The levels of plasma IL-33 and Da-jvGlu were detected in 126 patients of acute cerebral infarction and 10 normal controls.The correlation between IL-33 and cerebral infarction was analyzed.Results Compared with those in normal control group and the pretherapy groups,the levels of IL-33 in large volume were significantly higher (P<0.05).After giving Shenqi glucose injection,the level of IL-33 in mid volume and small volume were significantly higher between groups (P<0.05).Compared with those in normal control group and the pretherapy groups,the level of Da-jvGlu in large volume was significantly higher (P<0.05).After giving Shenqi glucose injection,there was no significant difference of Da-jvGlu in statics between groups (P>0.05).Conclusion Shenqi glucose injection can affect the levels of IL-33 to protect nerve cells and stabilize blood glucose concentration.
2.Causal relationship between gut microbiota and vestibular dysfunction: a mendelian randomization study
Journal of Apoplexy and Nervous Diseases 2023;40(11):983-988
Objective Previous research has indicated a discernible association between gut microbiota and vestibular dysfunction; however,further investigation is required to establish a causal relationship. This study aims to comprehensively study the causal relationship between gut microbiota and vestibular dysfunction and determine the species of related gut microbes. Methods A two-sample Mendelian randomization analysis was performed. The gut microbiome data of 18 340 individuals obtained from the genome-wide association study (GWAS) by the MiBioGen consortium in 2021 were used as the exposure samples. The GWAS data related to vestibular dysfunction were used as the outcome samples. The inverse variance weighting method was employed to analyze the gut microbiome data. The results were assessed based on the odds ratio(OR)as the effect index with 95% confidence interval(CI). The stability and reliability of the findings were assessed using the leave-one-out,heterogeneity,horizontal pleiotropy,and false discovery rate approaches. Results The results indicated a significant association between an elevated abundance of Bifidobacterium and a decreased risk of vestibular dysfunction. Furthermore,the leave-one-out method confirmed the stability of the results and the absence of instrumental variables exerting substantial influence on the findings. The causal relationship was negative. The influence of heterogeneity and horizontal pleiotropy on the causal effect could be eliminated. Conclusion Bifidobacterium may affect vestibular dysfunction by influencing 5-HT through the “gut-brain axis”,but this result still needs further verification.
3.Mechanism of Ferroptosis and Traditional Chinese Medicine Intervention on Neuronal Cell Ferroptosis After Stroke: A Review
Hanying XU ; Yibin ZHANG ; Dongmei ZHANG ; Jing LU ; Ying ZHANG ; Lei WU ; Tianye LAN ; Jian WANG
Chinese Journal of Experimental Traditional Medical Formulae 2022;28(20):232-240
Stroke is a common cerebrovascular disease, characterized by high incidence, mortality and disability rate. Neuronal cells, the basic unit of the central nervous system, can be injured to varying degrees when stroke occurs. Neuronal cell injury after stroke is also the key cause leading to neurological dysfunction, affecting the prognosis and quality of life of patients. Therefore, reducing the neuronal cell injury and delaying the process of cell death are effective to decrease the nerve function injury in stroke patients and improve their prognosis, thus lowering the death and disability rate of stroke. Ferroptosis is a new form of cell death that has been widely concerned in recent years. Several studies have confirmed that there is ferroptosis in neuronal cells after stroke. Since ferroptosis is an adjustable form, its intervention can help regulate the injury and death of neuronal cells. Studies have shown that inhibiting ferroptosis plays a role in protecting neuronal cells. Traditional Chinese medicine (TCM), with the multi-channel and multi-target treatment advantages, has been widely used in the whole stroke and has achieved good clinical efficacy. It might be a new direction taking TCM regulation of ferroptosis as the entry point for stroke treatment in the future. This review revealed the mechanism of ferroptosis, discussed the research status of TCM in intervening in neuronal cell ferroptosis, and provided reference for further improving the efficacy of TCM in stroke.
4.Effects of Hericium erinaceus Polysaccharide on the Content of Short-chain Fatty Acids in the Intestine of Ulcerative Colitis Model Rats
Yonggang YANG ; Dandan WANG ; Yanqiu ZHANG ; Mingxing WANG
China Pharmacy 2019;30(20):2763-2767
OBJECTIVE: To investigate the effects of Hericium erinaceus polysaccharide on the content of short-chain fatty acids (SCFAs) in the intestine of ulcerative colitis model rats. METHODS: A total of 40 SD rats were randomly divided into blank group, model group and H. erinaceus polysaccharide low-dose and high-dose groups (0.5, 1.0 g/kg), with 10 rats in each group. Except for blank group, other groups were given acetic acid enema to induce ulcerative colitis model. The next day after modeling, H. erinaceus polysaccharide groups were given relevant medicine solution intragastrically; blank group and model group were given relevant volume of water intragastrically, for 10 consecutive days. Colon tissue was collected, and pathological changes of colon tissue were observed by HE staining. The pathological changes of colon in rats were observed with naked eyes and scored so as to evaluate the therapeutic efficacy. The contents of 6 kinds of SCFAs (acetic acid,propionic acid,butyric acid,isobutyric acid,pentanoic acid and isovaleric acid) in intestine of rats were determined by GC-MS. RESULTS: Compared with blank group, inflammatory lesions of colonic mucosal epithelium in model group rats were obvious; colonic mucosal ulcer score increased significantly (P<0.01); the contents of 5 kinds of SCFAs and the total amount of SCFAs in colon except for propionic acid were decreased significantly (P<0.01). Compared with model group, the degree of colonic histopathological damage in H. erinaceus polysaccharide groups was significantly reduced; colonic mucosal ulcer scores were significantly reduced (P<0.05 or P<0.01); the contents of 5 kinds of SCFAs and the total amount of SCFAs in colon except for propionic acid were increased significantly (P<0.05 or P<0.01). CONCLUSIONS: H. erinaceus polysaccharide can effectively improve the pathological condition of ulcerative colitis model rats; its regulation of intestinal SCFAs (especially acetic acid and butyric acid) may be an important mechanism of its anti-ulcerative colitis.
5.Mechanism of Chaishao Liujunzi Tang in Treatment of Ulcerative Colitis Through TLR4/MyD88/NF-κB Signaling Pathway
Zhaopeng ZHANG ; Qiuying LI ; Ziqing YU ; Ru ZHANG ; Shuguang GUAN ; Junpeng GUO
Chinese Journal of Experimental Traditional Medical Formulae 2022;28(19):17-23
ObjectiveTo investigate the preventive and curative effect of Chaishao Liujunzi Tang (CSLJZT) on colonic mucosal injury induced by dextran sulfate sodium (DSS) in mice with ulcerative colitis (UC) and its mechanism. MethodFifty Balb/c male mice were randomly divided into normal group, model group, CSLJZT low-dose group, CSLJZT high-dose group, and sulfasalazine group. Except for the normal group, other groups were given 2.5% DSS freely for 7 d, and were given drug intervention after successful modeling for 7 d. Bodyweight, feces, and other general physiological statuses of mice were recorded every day, and disease activity index (DAI) scores were calculated.The colon length was measured, and stained by hematoxylin-eosin (HE) staining to observe the morphological changes of the colon.The enzyme-linked immunosorbent assay (ELISA) was used to determine the content of interleukin-1β (IL-1β), myeloperoxidase (MPO), and superoxide dismutase (SOD) in the serum. Western blot was used to determine the protein expression levels of Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), nuclear factor kappa B (NF-κB), inhibitor-kappa binding protein (IκB), Caspase-1, and nucleotide-binding oligomerization domain-like receptor 3 (NLRP3) in the colon tissues. ResultAs compared with the normal group, mice in the model group had significantly decreased body weight (P<0.01), severe diarrhea and hematochezia, and significantly increased DAI score (P<0.01). As compared with the model group, the decreasing trend of body weight was significantly alleviated in the CSLJZT groups (P<0.01), diarrhea and hematochezia were significantly improved, DAI score was significantly decreased (P<0.01), and colon length increased (P<0.05). HE staining showed that the pathological damage of colon tissue was significantly improved and the inflammatory cell infiltration was reduced in the CSLJZT groups as compared with the model group. As compared with the normal group, the serum levels of IL-1β and MPO were significantly higher (P<0.01) and SOD levels were significantly lower (P<0.01) of mice in the model group.Compared with the model group, the treated group reduced the serum IL-1β and MPO levels (P<0.01), and raised the SOD level (P<0.01). The results of Western blot showed that as compared with the normal group, the expression levels of TLR4, MyD88, NF-κB, Ccaspase-1, and NLRP3 proteins were significantly increased (P<0.01), whereas the expression level of IκB protein was significantly decreased (P<0.01) in the colonic tissue of mice in the model group. As compared with the model group, the expression levels of TLR4, MyD88, NF-κB, Caspase-1, and NLRP3 proteins were decreased (P<0.01), whereas the expression level of IκB protein was increased (P<0.01) in the colonic tissue of mice in the CSLJZT groups. ConclusionCSLJZT improves the inflammatory injury of the colon tissue in DSS-induced UC mice through TLR4/MyD88/NF-κB signaling pathway.
6.Anterior cruciate ligament reconstruction with the remaining bundle and ruptured fiber
Shu ZHANG ; Qiang ZHANG ; Changchun FAN
Orthopedic Journal of China 2006;0(08):-
[Objective]To investigate the technique and significance of arthroscopic anterior cruciate ligament(ACL) reconstruction with the remaining bundle and ruptured fiber.[Method]In 47 cases of ACL rupture verified with arthroscopy,there were 15 cases of partial disruption,26 cases of complete disruption,and 6 cases of ligament flabby.ACLs were reconstructed with single bundle of autogenous quadrupled hamstring tendons under arthroscopy without remove of the remaining bundle or remnants of ACL.The femur tunnel was fixed by the RigidFix system,and the Intrafix system was used to fix the tibia tunnel.[Result]All of 47 cases were followed up for an average of 19 months(ranged from 12 to 24 months).The stabilization was strengthened after operation.Lysholm knee function score increased by 26.3 scores,from average 68.6 scores of preoperation to average 94.9 scores after operation.Lachman test was negative in 43 cases,weakly positive in 3 cases and positive in 1 case.According to Insall knee therapeutic effect evaluation criterion,38 cases achieved excellent results and 9 cases achieved good results.[Conclusion]Arthroscopic reconstruction of ACL with the remaining bundle preserving technique could facilitate revasculization and reinnervation of the reconstructed ligament and maintain stability of knee joint.
7.Protective Effect of Wutou Chishizhi Wan on Vascular Endothelial Cells and Oxidative Stress in Myocardial Ischemia-reperfusion Rats
Meng-ni WANG ; Lu-lu XIE ; Zhao-peng ZHANG ; Bing LING ; Zhi-hui LIN ; Pai LIU ; Jun-peng GUO ; Hong-yan LIU
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(21):40-47
Objective:To investigate the protective effect of Wutou Chishizhi Wan on myocardial ischemia reperfusion injury (MIRI) in rats, and observe its effect on such mechanisms as coagulation function, vascular endothelial cells and oxidative stress in rats. Method:A total of 40 SD rats were randomly divided into normal group, model group, positive drug group (Urokinase group) and Wutou Chishizhi Wan group, with 10 rats in each group. Except for the normal group, rat myocardial ischemia-reperfusion injury models were established. The changes of heart rate (HR) at 10 min before ischemia, 30 min after ischemia and 30, 60, 120 min (T0,T1,T2,T3,T4), and the change of electrocardiogram (ECG) J point after modeling in rats were observed. The pathological changes of rat myocardial tissue were observed by hematoxylin-eosin (HE) staining. The changes of four indexes of coagulation [prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen content decreased significantly (FIB)] in rats were observed. The contents of endothelin-1 (ET-1), thromboxane A2 (TXA2) and prostacyclin (PGI2) in serum and myocardium levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) of MIRI rats were observed. Western blot assay was used for the detection of oxidative stress protein Keap1 and transcription factor-E2-related factor (Nrf2) expression levels in rat myocardial tissue. Result:Compared with the normal group, the ECG of MIRI rats showed significant myocardial ischemic injury-like changes, ST segment was significantly elevated, J point was significantly increased, and the incidences of HR in T1, T2, T3 and T4 were significantly reduced (
8.Efficacy and Mechanism of Qingre Lishi Prescription in Treating Children with Acute Bacterial Lower Urinary Tract Infection of Bladder Damp-heat Syndrome
Xin-yao ZHANG ; Jian LIU ; Ning JI ; Jin-hua FENG ; Wan-qi WANG ; Kai JIANG
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(10):51-57
Objective:To observe the efficacy of Qingre Lishi prescription in treating children with acute bacterial lower urinary tract infection of bladder damp-heat syndrome, and to explore its mechanism of action. Method:Eighty children with acute bacterial lower urinary tract infection of late bladder damp-heat syndrome who were admitted to the Affiliated Hospital of Changchun University of Chinese Medicine were divided into control group and observation group, 40 cases in each group. Patients in control group were given Bazhengsan for oral treatment on basis of basic treatment, while patients in observation group were given Qingre Lishi prescription for oral administration plus external washing treatment. After two weeks of treatment, the clinical and etiological effect, traditional Chinese medicine (TCM) syndrome scores, antipyretic time and urinary negative time, adverse reactions, and urine pathogens (
9.Analysis of the application of PONV intensity scale among the Chinese patients with laparoscopic operation
Liangjie ZHENG ; Chuzhou MA ; Changchun ZHANG
Chinese Journal of Primary Medicine and Pharmacy 2013;20(z1):8-10
Objective To esplore the effecf and the practical utility of the postoperative nausea and vomiting intensity scale for Chinese patients with laparoscopic operation .Methods Ninety patients with ASA I-II,Apfel≥2 were enrolled .Interviews were carried out at 6 and 24 hours postoperatively .Measurements included the PONV Inten-sity Scale,nausea and pain visual analogue scale .Quality of Recovery Score and antiemetic were used .The patients whose PONV intensity score ≥50 were assessed by PONV intensity scale and PONV VAS .Results There was signif-icant difference of the occurrence of clinical PONV between the patients with Apfel ≥3 and those with Apfel=2(P<0.05).The PONV Intensity Scale had a stronger correlation with PONV VAS ,r=0.946(P<0.01).The PONV In-tensity Scale had a stronger correlation with QOR,r=-0.937(P<0.01).Patients with clinically important PONV at 24 hours required more antiemetic therapy (P=0.024).PONV rate of the patients with a clinically significant score VS those without a clinically significant score was 80%VS 18%(P<0.05).Conclusion PONV intensity scale can distinguish trivial from clinically important PONV availably in Chinese Laparoscopic operation .It is more reliable than PONV VAS.Patients with clinically important PONV required more antiemetic therapy .Clinically important PONV ,as determined by the PONV Intensity Scale ,was associated with a poor quality of recovery .
10.Effect of Ranae Oviductus Protein Hydrolysate on Ethanol-induced L-02 Cell Injury
Yi ZHANG ; Ran ZHENG ; Qi YU ; Li-li JIAO ; Bo LI ; Da LIU ; Yi-ping LI
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(15):43-50
Objective:To study the protective effect and mechanism of Ranae Oviductus protein hydrolysate (ROPH) on the expression of pathway-related proteins in ethanol-induced L-02 cell injury. Method:The ROPH was prepared by compound enzymatic hydrolysis. L-02 cell injury model was induced with 400 mmol·L-1 ethanol. Cell viability was detected by cell counting kit-8 (CCK-8) assay. Cell cycle and apoptosis were examined by flow cytometry. JC-1/Hochest staining was employed for qualitative investigation. The expression of related proteins in apoptosis, mitogen-activated protein kinase (MAPK) signaling pathway, and pyroptosis in L-02 cells was detected by Western blot. Result:The results of the CCK-8 assay showed that 400 mmol·L-1 ethanol could induce L-02 cell injury within 12 hours. Compared with the blank group, the model group showed decreased viability of L-02 cells (