Objective To observe the influence on clinic symptom and pulmonary function in asthmatic patients treated with polysaccharide nucleicacid of calmette-guerin(BCG-PSN) injection in asthmatic patients.Methods 30 cases(treatment group) with additional BCG-PSN injection and 30 cases(control group) with only normal treatment were studied in contrast.They were observed in pulmonary function and clinic symptom before and after treatment.Results Symptom and pulmonary function improved in both groups.The validity rate in treatment group was 96.7%,and the control group was 80%.Conclusion BCG-PSN could alleviate the inflammation of bronchus and excellent effects of BCG-PSN injection in treatment on clinic symptom and pulmonary function of asthmatic patients were found.

Objective To investigate the incidence of dental fluorosis and pit caries in school-age children from binhai new area of Tianjin, and to discuss the relationship between dental fluorosis and pit caries, providing the guidance for the prevention and treatment of them. Methods Referring to WHO’s“Oral Health Surveys Basic Methods”(Fourth Edition) and protocols of the third national oral epidemiology investigation, 3 778 children aged 7 to 9 year-old with similar levels of education were investigated for their dental fluorosis and fissure caries using the cross sectional survey method. Results The data showed that the incidence of dental fluorosis was less than 10%in the samples, and the community fluorosis index (CFI) was 0.075. The caries prevalence rate and mean decayed missing filled tooth (DMFT) of first permanent molars were 15.57%and 0.46 in male students, and those were 17.41%and 0.58 in female students. There were no significant differences in the caries prevalence rate and DMFT of first molar between male and female children (χ2=2.345, P>0.05). There were statistically significant differences in the caries rate and DMFT between different age groups (χ2=172.576, P<0.05), and both increased with age. Conclusion After years of defluoridation project in Tianjin, the detection rate and index of dental fluorosis in children have showed a downward trend. The caries prevalence rate may be related to the eruption rate of the first permanent molar.

Objective　To explore the levels of peripheral blood IL-33 and the difference of arteriovenous fistula glucose (Da-jvGlu) after giving Shenqi glucose injection in acute cerebral infarction patients.Methods　The levels of plasma IL-33 and Da-jvGlu were detected in 126 patients of acute cerebral infarction and 10 normal controls.The correlation between IL-33 and cerebral infarction was analyzed.Results　Compared with those in normal control group and the pretherapy groups,the levels of IL-33 in large volume were significantly higher (P<0.05).After giving Shenqi glucose injection,the level of IL-33 in mid volume and small volume were significantly higher between groups (P<0.05).Compared with those in normal control group and the pretherapy groups,the level of Da-jvGlu in large volume was significantly higher (P<0.05).After giving Shenqi glucose injection,there was no significant difference of Da-jvGlu in statics between groups (P>0.05).Conclusion　Shenqi glucose injection can affect the levels of IL-33 to protect nerve cells and stabilize blood glucose concentration.

Objective:To measure the color values of Gentianae Radix et Rhizoma and the contents of gentiopicroside,the appearance index value of the representative color was correlated with the intrinsic index value representing the quality,in order to explore the correlation between the contents of active ingredients and the color values,and provide basis for the quality evaluation of Gentianae Radix et Rhizoma. Method:The color difference values of Gentianae Radix et Rhizoma powder was measured by colorimeter.The content determination method of gentiopicroside in the 2015 edition of China Pharmacopoeia was adopted. The content of gentiopicroside was determined by HPLC,and the correlation and regression analysis was carried out by SPSS 21.0 software. Result:There was a significantly positive correlation between the contents of gentiopicroside and L^* (representing colorshade)and E^* ab(representing total color difference)(P<0.01),while the contents of gentiopicroside and a^* (representing color red-green direction) and b^* (representing color yellow-blue direction) had a significantly negative correlation (P<0.01). Conclusion:The color value of Gentianae Radix et Rhizoma has a certain correlation with the content of gentiopicroside,and yellowish Gentianae Radix et Rhizoma contain more active ingredients, with a better quality.The contents of gentiopicroside active ingredients in Gentianae Radix et Rhizoma can be quickly predicted by determining the color difference values,which can provide a new idea for quality evaluation of this herb.

Objective Previous research has indicated a discernible association between gut microbiota and vestibular dysfunction； however，further investigation is required to establish a causal relationship. This study aims to comprehensively study the causal relationship between gut microbiota and vestibular dysfunction and determine the species of related gut microbes. Methods A two-sample Mendelian randomization analysis was performed. The gut microbiome data of 18 340 individuals obtained from the genome-wide association study （GWAS） by the MiBioGen consortium in 2021 were used as the exposure samples. The GWAS data related to vestibular dysfunction were used as the outcome samples. The inverse variance weighting method was employed to analyze the gut microbiome data. The results were assessed based on the odds ratio（OR）as the effect index with 95% confidence interval（CI）. The stability and reliability of the findings were assessed using the leave-one-out，heterogeneity，horizontal pleiotropy，and false discovery rate approaches. Results The results indicated a significant association between an elevated abundance of Bifidobacterium and a decreased risk of vestibular dysfunction. Furthermore，the leave-one-out method confirmed the stability of the results and the absence of instrumental variables exerting substantial influence on the findings. The causal relationship was negative. The influence of heterogeneity and horizontal pleiotropy on the causal effect could be eliminated. Conclusion Bifidobacterium may affect vestibular dysfunction by influencing 5-HT through the “gut-brain axis”，but this result still needs further verification.

Objective:To investigate the effect of Yisui Jiedu prescription on hippocampal neuron damage in vascular dementia (VD) rats and to regulate phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/recombinant Bcl-2 associated death promoter (Bad) mechanisms of signaling pathways of neuronal apoptosis. Method:The 40 SD rats were divided into sham operation group， model group， donepezil hydrochloride group and Yisui Jiedu prescription group， with 10 rats in each group.VD animal model was prepared by bilateral carotid artery permanent ligation (2-VO) method.The sham operation group and the model group were intragastrically administered with normal saline， the donepezil hydrochloride group was intragastrically administered with donepezil hydrochloride 0.52 mg·kg^-1. The Yisui Jiedu prescription group was administered with Yisui Jiedu prescription (11.11 g·kg^-1)， 1 time/d . After 30 days， Morris water maze was used to test the learning and memory ability of rats， hematoxylin-eosin (HE) staining was used to observe the histomorphological structure of hippocampal CA1 region. Ultrasound of neuron in rat hippocampal CA1 region was observed by transmission electron microscopy (TEM).Real-time fluorescent quantitative（Real-time PCR） was used to detect the Akt， Bad mRNA expression.Western blot was used to detect the Akt， p-Akt and Bad protein expression in hippocampus. Result:Compared with sham operation group， the learning and memory ability of model group decreased significantly(P<0.05). The pathological structure and neuronal ultrastructure of the hippocampus were changed obviously. Hippocampal tissue Akt mRNA and the Akt，p-Akt protein expression level decreased significantly(P<0.05)， and the levels of Bad mRNA and protein were significantly increased (P<0.05). Compared with model group， Yisui Jiedu prescription group can significantly improve the learning and memory ability of rats， improve the neuronal cells and ultrastructural changes in hippocampal CA1 area，and increase the expression of Akt mRNA and Akt，p-Akt protein in hippocampus. Decreased Bad mRNA and Bad protein expression levels (P<0.05). Conclusion:Yisui Jiedu prescription can significantly improve the learning and memory ability of VD rats， improve the ultrastructural pathological changes of hippocampus and neurons， and repair damaged neurons， which may promote Akt phosphorylation and activate PI3K/Akt/Bad. The signaling pathway plays a role in the defense of neurons against apoptosis.