OBJECTIVE To optimize the processing technology of Huechys sanguinea, and establish the quality standard of rice-fried Huechys sanguinea. METHODS Taking the exudates, scent, color, degree of crushing, and protein content as the evaluation indicators combining entropy weight method with analytic hierarchy process to establish a comprehensive evaluation method. The effects of frying time, temperature and the ratio of medicinal materials to excipients on the quality of rice-fried Huechys sanguinea were investigated, and an orthogonal test to optimize the best processing technology was established. Microscopic identification and thin-layer identification of rice-fried Huechys sanguinea, determination of its moisture, ash, extract and heavy metals, and establishment of quality standards. RESULTS The best processing process for Huechys sanguinea was after the rice with an excipient ratio of 1∶2 was frying and smoking, pour processed Huechys sanguinea, stir fry at 140 ℃ for 6 min. The extract content was 199 mg·g-1, the odor, color, and fragmentation scores were all 5, and the protein content was 136 mg·g-1. The moisture content of rice-fried Huechys sanguinea was 2.97%, the total ash content and acid insoluble ash content were 3.79% and 0.33% respectively, the contents of heavy metals lead, cadmium, arsenic, mercury and copper were 2.26, 1.91, 0.06, 0.13 and 38.14 mg·kg-1 respectively, and the aflatoxin B1, B2, G1 and G2 were all lower than the detection limit by 0.1 μg·kg-1, the content of extract was 20.10%. CONCLUSION The processing technology of Huechys sanguinea is reliable and stable, which provides a scientific basis for standardizing the processing technology and quality standards of Huechys sanguinea decoction pieces. It is suggested that the moisture content of rice-fried Huechys sanguinea is <3.56%, total ash and acid insoluble ash are <4.55% and 0.40% respectively, the content of heavy metal lead is <2.71 mg·kg-1, cadmium is < 2.29 mg·kg-1, arsenic is <0.07 mg·kg-1, mercury is <0.16 mg·kg-1, copper is <45.77 mg·kg-1, the content of extract is >16.08%, and aflatoxin B1, B2, G1, G2 are <0.1 μg·kg-1.

Based on fairly systematic and complete information networks, the software Multimedia Teaching and Clinical Practice of Hospital Infection Control was developed. By adopting information driven network and digital hospital infection management, traditional ideological concepts, operational methods and modes of training were transcended. As a result, ways of communication became more flexible, intermediate links were reduced, and the efficiency of management was enhanced.

Objective To explore the possible anti-inflammatory mechanism of intensive insulin therapy (IIT) by studying the effect of IIT on the levels of TNF-?,IL-6,C-reactive protein (CRP) and APACHE Ⅱ score in biliary pyemia.Methods Twenty eight patients with biliary pyemia who were admitted by our department and given an operation within 24 h form Jan.2005 to Dec.2008 were randomly divided into two groups by using random number table numbers:one group treated with IIT (IIT group,n=14) and another group treated with routine insulin therapy (RIT group,n=14).The inflammatory factors,such as TNF-?,IL-6 and CRP were detected dynamically and the APACHEⅡ score was calculated.Results The level of CRP and APACHEⅡ score on day 5 and 7 and the levels of TNF-? and IL-6 on day 3,5 and 7 after operation in IIT group were significantly lower than those in RIT group (P

Objective: To establish the model of palatine mucoperiosteal expansion and observe a new possible treatment approach to soft tissue defect of cleft palate. Methods: Expanders of 10ml were implanted beneath the palatine mucoperiosteum in 4 dogs and injected 1.0ml 0.9% NaCl at the same time. 20 days after the placement of the expander, the expander was expanded by 1.0-1.5ml every 7 days again. The dogs were killed after the expanders reached their capacity. All the specimens were performed histomorphometric and histological examinations. Results: Histological results showed the increase in the layer of cell and the number of collagen fibra and blood vessel. All the specimens of expanded tissue showed the formation of extra palatine mucoperiosteal tissue. Conclusion: Expansion technique is able to form extra palatine mucoperiosteal tissue,which could be a new restoration approach to soft tissue defect of cleft palate and others.

Objective: To study the structures of ginseng glycopeptides, and to explore the protective effect on the apoptosis of PCI 2 cells treated with amyloid beta25-35 (Aβ25-35), and to lay a theoretical foundation for the development of ginseng anti-Alzheimer' s disease (AD) drugs. Methods: The structures of ginseng glycopeptides were analyzed by reversed-phase high performance liquid chromatography coupled with Q Exactive Orbitrap mass spectrometry. The PCI2 cells were divided into 6 groups, and treated with the medium including different concentrations 0, 6. 25, 12. 50, 25. 00, 50. 00, and 100. 00 mol • L_ 1 ) of Aβ25-35 , and the survival rates of PC12 cells were measured by cell counting kit-8 (CCK-8) method. The PC12 cells were divided into blank control group, model group, and ginseng glycopeptides administration group. The medium including 50. 00 jumol • L_ 1 Aβ25-35, was added in model group, and different concentrations 0. 03, 0.10, 0. 30, and of 1. 00 g • L_ 1 ) ginseng glycopeptides+ 50 jumol • L_ 1 Aβ25-35 were added in administration groups. The survival rates of PC12 cells were measured by CCK-8 method. The apoptotic rates of PCI 2 cells after treated with glycopeptides and Aβ25-35, were detected by Annexin V-FITC/PI method. The PCI 2 cells were divided into blank control group, model group (50.00 jumol • L_ 1 Aβ25-35) and different concentrations (0 . 1 0 and 0.30 g • L_ 1 ) of ginseng glycopeptides administration groups; the percentages of PCI 2 cells in different cell cycles were tested by flow cytometry. Results: More than 20 glycopeptide structures were obtained, such as the peptide chain was NLSHYHSGSS, the glycosyl group was Nl-HexNAc, and the peptide chain was SGSSSSSSSEDDGMGR, the glycosyl group was S6-HexNAc. When the concentration of Aβ25-35 was 50 jumol • L_ 1 , the survival rate of PC12 was (55. 45 + 2. 3 4) % and the survival rate was significantly lower than that in blank control group (P < 0 . 0 1) . Compared with blank control group, the survival rate of the PC12 cells in model group was significantly decreased (P < 0 01); compared with model group, the survival rates of the PCI2 cells in administration groups were significantly increased (P< 0. 05). Compared with blank control group, the apoptotic rate of the PC12 cells in model group were increased (P < 0. 01); compared with model group, the apoptotic rates of the PC12 cells in administration groups were decreased (P < 0 . 05). Compared with blank control group, the percentage of PC12 cells in S phase in model group was increased (P