Poria cocos, which refers to the dry sclerotium of Poria cocos (Schw.) Wolf, is used as a traditional Chi-nese medicine (TCM). This review summarized the chemical constituents of P. cocos, their bioactivity and the extrac-tion technology of polysaccharides and triterpenes in order to provide in-depth study on the development and appli-cation of P. c oc os .

Objective To investigate the effect of butylphthalide on the memory,movement and ERP (event-related potentials) in patients with cerebral infarction cognitive impairment.Methods A total of 134 patients with cerebral infarction cognitive impairment were randomly divided into treatment group (67 patients) and control group (67 patients).All patients were given conventional cerebral infarction treatment as well as cognitive improvement intervention.Oral administration of butylphthalide was added onto the patients in the treatment group.Curative effects were observed after 30 days′ continued treatment.The cognitive function scores,memory scores,movement scores,ERP test and adverse reaction were compared between the two groups before and after treatment.Results After 30 days′ treatment,two groups of the montreal cognitive assessment scale (MoCA) scores were 27.2±4.9 and 25.1±4.1 respectively;the total scores of memory were 5.5±0.5 and 4.9±0.5 respectively;the scores of motor assessment scale (Fugl-meyer) were 85.6±6.2 and 74.2±6.1 respectively and P3 amplitudes were (9.5±0.9)μV and (8.1±0.9)μV respectively.All the indexes mentioned above in treatment group were significantly increased compared with those of before treatment(P<0.05).The results of N1,N2,P2 and P3 in the latency detection were significantly decreased compared with those of before treatment(P<0.05).And the results of P2 and P3 in the latency detection of the treatment group were shorter than those of the control group(P<0.05).After treatment,no adverse reaction was observed in both groups.Conclusion Based on conventional cerebral infraction treatment,oral administration of butyraldehyde effectively improve the therapeutic effect.

Aim To study the proliferative effect of resveratrol pretreatment on oxygen-glucose deprivation/reoxygenation ( OGD/R ) injury of rat cortical neural stem cells ( NSCs ) in vitro. Methods Isolation and purification of NSCs in neonatal Sprague-Dawley( SD) rats were conducted by suspended cultivation. The third passage NSCs of adherent culture was cultured under oxygen and glucose deprivation for 150 min and reoxygenation for 24 h. The experimental subjects were divided into normal, control, ethanol and resveratrol pretreatment groups. Immunofluorescence was used to identify NSCs. Cell viability was detected with CCK-8 assay. Flow cytometry cell cycle and BrdU assay were used to measure cell proliferation. Results Cells both in suspended and adherent cultivation highly expressed neuroepithelial stem cell protein ( nestin ) . Compared with the control group, NSCs viabilities and prolifera-tion in resveratrol groups (1, 5, 20 μmol·L-1 ) were significantly heightened, and highest in the 5 μmol · L-1 resveratrol group ( P<0. 05 ) . Conclusion Res-veratrol pretreatment can reduce injury and promote proliferation of NSCs after oxygen-glucose deprivation /reoxygenation.

Objective To evaluate diagnostic significance of renal arteriography by injecting adrenalin in patients with renal tumour.Methods The renal angiography after administration of adrenalin (6 ?g) in 47 patients with renal malign ant tumour were performed.The angiographic results were analysed in comparison with that of pathology.The diagnostic accuracy parameters including sensitivity,specificity,accuracy,positive prediction rate,negative prediction rate,positive and negative index were caluclated.Results The sensitivity, specificity and accuracy in the detection of renal malign ant tumour were 93% ,94% and 94% respectively. Positive prediction rate were 97%,negative prediction rate was 89%, positive likelihood rate was 15.8, negative likelihood rate was 0.07 and Youden index was 0.87.Conclusion Medicaments angiography by injecting adrenalin is of important diagnostic value for renal tumour,especially for those which are vascularized and could not diagnosed by other technology qualitatively.

Objective To observe the levels of plasma and urinary urotensin Ⅱ in patients with Type 2 diabetes mellitus (DM)and to investigate the influence of renal impairment and other involved clinical or laboratory feature on them. Methods The 42 patients with type2 patients were divided into three groups according to their correctional clearance of creatinine(Ccr):Group A(n=14)with Ccr≥70 mL/min; group B(n=18)with 30≤Ccr

Objective To explore the curative effect on superselective uterine artery embolization for treatment of placenta inereta.Methods Pelvic arteriography was performed to confirm bleeding vessels.Then a 5 F Cobra catheter was inserted superselectively into uterine artery ipsilateral to bleeding,through which methotrexatum(MTX)and gelatin sponge were injected for embolization.After the procedure,bleeding,blood pressure,dischargement of placenta tissue,uterine recuperation,and plasma β-HCG were monitored.Results Bleeding vessels were confirmed in all of the 5 cases of placenta increta.Uterine artery embolization was successful at sole procedure.The operation time was 25.0 to 60.0 min.with the mean time (37.4±5.8)min.Vaginal bleeding stoped in 3.0 to 12.0 minutes after embolization and the mean time was(5.7±2.4)min.Blood pressure returned to normal after operation and vital signs were stable.Placenta tissue discharged on the 5th day to the 4th week after embolization and the mean time was 17 d.The uterus recuperated and blood β-HCG recovered simutaneously.The menstruation and ovulation during follow-up returned to normal.Conclusion Superseleetive uterine artery embolization for treatment of placenta increta has advantages such as short operation time,minimal invasion,definite curative effect and reservation of uterus,which is worthy in clinical application.

Objective To detect the characteristics and in vitro cell compatibility of human acellular dermal matrix (ADM) with the improved method.Methods Cell components of healthy human skins were removed by the improved method and the traditional method respectively.The porosity, degradation time in vitro of the ADM prepared by two methods and the cytotoxicity of the material infiltration liquid with the improved method on the adipose derived stem cells were detected.HE staining was used to detect the residual of the cells, the integrity of collagen and cell biocompatibility.Scanning electron microscopy (SEM) was used to detect the pore size.Results Both the two methods could completely remove the cells, and maintain the integrity of the collagen scaffold;The porosity of ADM with the improved method was higher (93.1±1.02)% than that of traditional method (74.27±2.04)% (P<0.05);There was no significant difference in the cytotoxicity and in vitro degradation time between the two kinds of ADM;While pore diameter of the improved method was significantly higher [(181.21±66.9) μm] than that [(102.38±15.63) μm] in dermal reticular surface with the traditonal method (P<0.05).Conclusions There is no obvious cytotoxicity of the ADM with the improved method, and therefore it is more suitable for cell adhesion growth with higher porosity and larger pore size.

Objective To explore the possibility of primary culture of human adipose-derived stem cells in vitro and differentiation induction into epidermal cells.Methods Adipose-derived stem cells were primarily cultured by enzyme digestion method.The expression of CD29,CD34,CD44,CD49d,CD80 and CD106 was detected by immunohistochemical staining.ADSCs differention into epidermal cells in vitro were under induction medium.Proliferation activity and morphology of cells of induction group and control group were observed,and the expression of CKs in the two groups were also analyzed.Results ADSCs were successfully isolated and cultured from human liposuction tissue.It was revealed by immunofluorescence staining that ADSCs possessed specified expression of surface antigen of stem cells; ADSC successfully differentiated towards epidermal cells in vitro and possesed considerable high-level reproductive activity,cell morphology and expression of CKs also shown the tendency of differentiated into epidermal cells.Conclusions ADSCs can be isolated and cultured from human liposuction tissue,and can proliferate persistently.ADSCs possess specified expression of surface antigen.ADSCs can also be differentiated in vitro to the direction of epidermal cells.

Objective To explore the effect of epidermal growth factor (EGF) on tube formation of HUVEC induced by the secretion of angiogenesis factors of adipose-derived stem cells (ADSCs).Methods ADSCs were primarily cultured by enzyme digestion method.The flow cytomertry was performed to detect the expression of cell surface marker.ELISA was used to detect the expression of VEGF,HGF,and SDF-1 after given different doses of EGF.Tube formation assay was used to examine the effect of EGF on the tube formation induced by ADSCs.Results ADSCs were successfully isolated and cultured from human liposuction tissue and specific markers were expressed on ADSCs.EGF promoted the secretion of angiogenesis factors VEGF,HGF,and SDF-1,which were secreted by ADSCs.EGF pretreatment increased the ability of tube formation of HUVECs induced by ADSCs.Conclusions ADSCs induce the secretion of angiogenesis factors in vitro,and thus increase the ability of tube formation of HUVECs.EGF promotes the secretion ability of ADSCs,and the best concentration is 15 mg/L.

A total of 38 cases of senile brucella spondylitis disease at our hospital during January 2002 and March 2012 were analyzed .After admission , all of them were definitely diagnosed on the basis of epidemiological history , clinical manifestations , laboratory tests , imaging and pathological examinations . Over a follow-up period of 12 months, 17 cases were cured after standardized drug treatment .Among 21 surgical cases, there were curing (n=17) and improving (n=2).Senile brucellosis spondylitis has distinct serological and pathological characteristics .And formulating the diagnostic criteria may improve its diagnostic rate and reduce its misdiagnostic rate .And standardized drug therapy achieves a better curing rate and a proper timing of surgical intervention improves its clinical outcomes .