2.Expression, Purification of Human Endostatin in Pichia pastoris and the Detection of Its Anti-angiogenic Activity
Xue-Jing YAO ; Zhuang-Lin LI ; Guo-Dong CHANG ; Gui-Yong YUAN ; Xue- YU ;
China Biotechnology 2006;0(04):-
Endostatin is a potent, endogenous inhibitor of angiogenesis, which corresponds to the C-terminal fragment of collagen ⅩⅧ. The human endostatin gene was amplified from a human fetal liver cDNA library by means of PCR and was cloned into pPIC9K vector. Soluble endostatin was superexpressed in Pichia pastoris (50.5mg/L) . The recombinant protein was purified by cation exchange chromatography with the final yield of more than 95%. Western blotting analysis showed positive immunoreactivity to the expressed product. Recombinant endostatin was able to suppress the angiogenesis on the CAMs. Also, the endostatin inhibited specifically of the migration of HMECs stimulated by bFGF, with EC50 being about 0.4 ?g/ml.
3.Clinical comparison of two microinvasive surgery for giant benign prostatic hyperplasia
Zhenyu ZHOU ; Yong ZHANG ; Guangning WANG ; Chuanfeng XUE ; Cunquan QIU ; Song CHANG ; Keling HE
Chinese Journal of Postgraduates of Medicine 2012;35(5):30-33
Objective To compare the clinical effect between retropubic extraperitoneal laparoscopic prostatectomy with prostatic urethra preservation and transurethral resection of the prostate (TURP) for giant benign prostatic hyperplasia (BPH).MethodsThe clinical data and follow-up of 128 cases of giant BPH were analyzed retrospectively.Seventy-two cases underwent TURP (TURP group) and 56 cases underwent retropubic extraperitoneal laparoscopic prostatectomy with prostatic urethra preservation ( laparoscopic group ).The operation time,blood loss,gland mass excision,bladder washing time,catheterization time,hospital stay,hospital cost,international prostate symptoms score (IPSS) and quality of life questionnaires (QOL),maximum flow rate (MFR),residual urine volume (RUV) were compared between two groups.ResultsThe bladder washing time,catheterization time,hospital stay in laparoscopic group were less than those in TURP group[0 d vs.(2.8 ± 1.2) d,(2.3 ± 0.6) d vs.(5.2 ± 1.5) d,(4.2 ± 0.5) d vs.(7.5 ±0.5) d],gland mass excision in laparoscopic group was more than that in TURP group [(100.2 ±25.4) g vs.(85.6 ± 15.5) g],there were significant differences between two groups(P< 0.05).There was no significant difference in the operation time,blood loss,hospital cost between two groups (P > 0.05).There were significant differences in IPSS,QOL,RUV and MRF before and after 3,6 months treatment for two groups [ laparoscopic group:(9.1 ± 3.4),(7.5 ± 2.5 ) scores vs.(27.5 ± 5.8) scores,( 1.8 ± 1.1 ),( 1.6 ± 0.8)scores vs. (5.5 t0.5) scores,(26.5 ± 11.5),(22.4 ± 12.6) ml vs. (145.0 ±48.0) ml,(17.6 ±8.4),(20.2 ± 5.4) ml/s vs.(8.3 ± 3.5) ml/s;TURP group:(9.2 ± 3.8),(7.8 ± 2.2) scores vs.(28.5 ± 5.4) scores,( 1.9 ± 1.2),( 1.7 ± 0.6) scores vs.(5.0 ± 0.5 ) scores,(28.5 ± 12.9),(23.0 ± 11.7) ml vs. ( 155.0 ± 47.0) ml,( 17.8 ± 9.2),( 19.8 ± 4.5 ) ml/s vs.(7.2 ± 3.2 ) ml/s ] (P < 0.01 ),but there was no significant difference between two groups (P > 0.05).ConclusionsThe clinical effect of two microtrauma surgery are good.Laparoscopic technique is a feasible treatment option for patients suffered from giant BPH for which has the benefit of a quicker recovery,shorter hospital stay,less complications,no bladder washing.
5.Effect of Washing Respiratory Way with Ambroxol Hydrochloride on Respiratory Mechanics in Premature Infant with Ventilation
hong-bing, CHEN ; ji-chang, CHEN ; xiao-xue, XIE ; cui-yu, LI ; yong-jiang, JIANG
Journal of Applied Clinical Pediatrics 1992;0(06):-
Objective To observe the effect of using Ambroxol hydrochloride(AM)to wash respiratory way to treat neonatal respiratory distress syndrome(NRDS) in ventilator,to explore dynamic changes of respiratory mechanics after using AM to wash respiratory way.Methods Thirty premature infants were chosen according with diagnosis criterion,which were randomly divided into 2 groups: NS group(n=15);AM group(n=15).Both NS and AM groups were treated with Babylog 8 000 ventilator,and routine treating and nursing,NS group was given for washing respiratory way in NS group,whereas AM was done in AM group.Pulmonary compliance(C),time constant(Tc),respiratory resis-tance(R),C20/C and minute volume(MV)were observed in both groups.Blood gas was routinely checked after 1 h ventilation treatment,and X ray was shot after 24 h.Results Pulmonary C significantly increased in weaning than that of beginning ventilation(P0.05),MV significantly increased in group AM than NS,respectively[(0.56?0.12) L/min and(0.35?0.11) L/min(P0.05).But ventilator-treating-time was markedly shorter in group AM than NS,respectively(60.52?6.23) h and(98.21?5.82) h(P
6.Exploring effective components of laxative effect of Anemarrhenae Rhizoma based on Chinese herbal processing theory.
Xia LEI ; Jie ZHANG ; Yuan LI ; Qiu-hong WANG ; Juan XUE ; Xiao-lin SU ; Chang-fu WANG ; Yong-gang XIA ; Hai-xue KUANG
China Journal of Chinese Materia Medica 2015;40(7):1283-1286
Anemarrhena asphodeloides processed by salt and raw product was compared including both chemical composition and laxative function in order to find the possible active substance to cure constipation. Processed and raw Anemarrhenae laxative effect on experimental constipation models was observed as well as chemical composition using UPLC-MS technology and the total sugar content was determined by phenol sulfuric acid method. Processed Anemarrhenae water extract improved excrement more than raw which has significant difference compared with the blank group (P < 0.05). On the other hand, the total ion flow spectrum showed no significant difference in most substance, but the total sugar content was significantly higher than raw product. Anemarrhenae ancient be recognized benefitting for draining body water in traditional Chinese medicine which has been lost in modern books because it is manifested as excellent laxative effect not diuretic effect. Saccharides carbohydrate may have closely relationship with this magically effect.
Anemarrhena
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chemistry
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Animals
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Chemistry, Pharmaceutical
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Constipation
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drug therapy
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physiopathology
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Defecation
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drug effects
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Drugs, Chinese Herbal
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administration & dosage
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chemistry
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Humans
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Laxatives
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administration & dosage
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chemistry
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Male
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Rats, Wistar
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Rhizome
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chemistry
7.DNA barcoding identification of Ginseng Radix et Rhizoma and Panacis Quinquefolii Radix based on trnL-trnF sequences.
Xiao-na SONG ; Xuan GU ; Chun-sheng LIU ; Yan-peng LI ; Xue ZHANG ; Yuan ZHANG ; Yong LIU ; Chang-hua MA
China Journal of Chinese Materia Medica 2015;40(10):1914-1918
To optimize indices of molecular identification for authentication of Ginseng Radix et Rhizoma and Panacis Quinquefolii Radix, four indices, including sequence similarity, specific positions, genetic distance and phylogenetic tree, were compared based on trnL-trnF sequences. Total DNA was extracted from Ginseng Radix et Rhizoma and Panacis Quinquefolii Radix, and trL-trnF sequences were amplified and sequenced. Sequence similarity was calculated by BLAST analysis. Specific positions were compared by DNAman software. Genetic distance and phylogenetic tree were analyzed by Mega software. The results showed that the inter-specific and intra-specific similarity of P. ginseng and P. quinquefolius respectively was 100% and 99. 6%. There were four specific positions at G153A, T463A, C732G and T818C. The inter-specific genetic distance (0) of trL-trnF sequences was lower than intra-specific genetic distance (0. 004). P. ginseng can be distinguished from P. quinquefolius based on the phylogenetic tree. It is concluded that Ginseng Radix et Rhizoma and Panacis Quinquefolii Radix can be authenticated by identification indices of sequence similarity, specific positions, genetic distance and phylogenetic tree. Index of specific positions based on trnL-trnF sequences is the most efficient index to authenticate Ginseng Radix et Rhizoma and Panacis Quinquefolii Radix.
Chloroplasts
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genetics
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DNA Barcoding, Taxonomic
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methods
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Panax
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classification
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genetics
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Phylogeny
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Plant Proteins
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genetics
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Rhizome
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classification
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genetics
8.Experimental study of the inhibitory effects of biotic royal jelly on the growth of ascitic hepatoma cell H22 in mice
Yong-Zi WANG ; Chang-Quan LING ; Xue-Qiang HUANG ; Pin-Rong PAN
Academic Journal of Second Military Medical University 2001;22(4):357-359
Objective: To observe the inhibitory effects of biotic royal jelly on the ascitic hepatoma cell H22. Methods: Mice bearing H22 tumor were fed on different types of royal jelly: No.1, 2 and 3. Their anti-tumor effects were observed in vivo. The general royal jelly and normal saline were observed as control. Results: Among these biotic royal jelly, the biotic royal jelly No.1 showed obvious tumor-inhibiting and survival-prolonging effects. In addition, it increased the number of WBC and augmented the amount of IL-2 and IFN-γ; the pathological study also indicated the denaturing and necrosis of most tumor cells with nuclei constraining and cell membrane rupturing, and large amount of lymphocytes and plasmacytes infiltrating around the mass. Conclusion: The No.1 biotic royal jelly has obvious anti-tumor effect, and it may take effects by inhibiting or killing tumor cells and improving the immunity of the host.
9.Comparative analysis of CT imaging findings on pulmonary alveolar proteinosis and pneumoconiosis
LI Zhong xue ZHAO Xia zi LI Qian chang HUANG Shi wen TANG Yong zhong QIU Kun hai
China Occupational Medicine 2022;49(06):683-686
Objective
To analyze the difference in computed tomography (CT) imaging findings between pulmonary alveolar
pneumoconiosis Methods
proteinosis (PAP) and occupational pneumoconiosis (hereinafter referred to as ). A total of 44 patients
with PAP (PAP group) and 44 patients with pneumoconiosis (pneumoconiosis group) were selected as study subjects using
Results
convenient sampling method. The CT images of these two groups were comparatively analyzed. The detection rates of
- -
pulmonary CT pattern changes such as map like performance, ground glass opacity, paving stone sign and sphenoid wing like
vs vs
changes of pulmonary hilum in the PAP group were higher than those in the pneumoconiosis group (77.3% 0.0%, 75.0%
vs vs P
2.3%, 56.8% 0.0%, 18.2% 0.0%, all <0.01); the detection rates of lymphadenopathy and calcification of pulmonary hilum,
small pulmonary nodules, emphysema and interlobular septal thickening were lower in the PAP group than those in the
vs vs vs vs P Conclusion
pneumoconiosis group (34.1% 100.0%, 4.5% 100.0%, 2.3% 45.4%, 0.0% 22.7%, all <0.01). Paving
-
stone sign and map like performance were most commonly found in the CT imaging of patients with PAP, and it is uncommon in
pneumoconiosis. These changes could be used as the CT differential diagnosis of the two diseases.
10.Comparative analysis of CT imaging findings on pulmonary alveolar proteinosis and pneumoconiosis
LI Zhong xue ZHAO Xia zi LI Qian chang HUANG Shi wen TANG Yong zhong QIU Kun hai
China Occupational Medicine 2022;49(06):683-686
Objective
To analyze the difference in computed tomography (CT) imaging findings between pulmonary alveolar
pneumoconiosis Methods
proteinosis (PAP) and occupational pneumoconiosis (hereinafter referred to as ). A total of 44 patients
with PAP (PAP group) and 44 patients with pneumoconiosis (pneumoconiosis group) were selected as study subjects using
Results
convenient sampling method. The CT images of these two groups were comparatively analyzed. The detection rates of
- -
pulmonary CT pattern changes such as map like performance, ground glass opacity, paving stone sign and sphenoid wing like
vs vs
changes of pulmonary hilum in the PAP group were higher than those in the pneumoconiosis group (77.3% 0.0%, 75.0%
vs vs P
2.3%, 56.8% 0.0%, 18.2% 0.0%, all <0.01); the detection rates of lymphadenopathy and calcification of pulmonary hilum,
small pulmonary nodules, emphysema and interlobular septal thickening were lower in the PAP group than those in the
vs vs vs vs P Conclusion
pneumoconiosis group (34.1% 100.0%, 4.5% 100.0%, 2.3% 45.4%, 0.0% 22.7%, all <0.01). Paving
-
stone sign and map like performance were most commonly found in the CT imaging of patients with PAP, and it is uncommon in
pneumoconiosis. These changes could be used as the CT differential diagnosis of the two diseases.