Objective_To study the significance of fascin protein expression in ductal intraepithelial neoplasia ( DIN) and invasive ductal carcinoma ( IDC) .Methods_Thirty cases of usual ductal hyperplasia ( UDH) , 30 ca-ses of flat epithelial atypia (FEA,DIN1a), 15 cases of atypical ductal hyperplasia (ADH,DIN1b), 2 cases of DCIS grade1(DIN1c),10 cases of DCIS grade2(DIN2)and 18 cases of DCIS grade3(DIN3)were immunohisto-chemically investigated using monoclonal antifascin antibody and compared with 120 cases of invasive ductal carci-noma (IDC).Results_Fascin protein expression was not found in normal breast tissue, UDH, FEA (DIN1a), ADH(DIN1b),DCIS grade1(DIN1c)and DCIS grade2(DIN2),but only seen in 2 of 18 cases of DCIS grade3 (DIN3)(11.1%), and 18 out of 120 cases of IDC (15%).Fascin protein expression was correlated with ER neg-ative( P<0.05) , PR negative( P<0.05) , tumor grade( P<0.05) and axillary lymph node status( P<0.05) , but not correlated with age,location,tumor sizeand HER2 expression.Conclusions_Fascin protein expression seems to be a late event, usually present in carcinoma.Targetting the fascin pathway may be a noval therapeutic strategy of mammary carcinoma.

Objective To explore whether depressive disorder is one of risk factors for coronary artery disease (CAD) in enrolled patients and observe the level of inflammation markers in coronary artery disease patients with depression. Methods In all patients, we recoded clinical information and data from Hamilton Depression Rating Scale for Depression( HRSD)and measured concentration of monocyte chemoattractant protein-1 (MCP-1), tumour necrosis factor α (TNFα) and hypersensitive C-reaction protein (hsCRP). Results Among 87 patients with coronary artery disease, depressive disorder was diagnosed in 26 patients, the prevalence of depressive disorder was 29. 9%. Among 101 patients without coronary artery disease, 12 patients were suffering from depressive disorder, the prevalence of depressive disorder was 11.90%. The prevalence of depressive disorder in coronary artery disease group was statistically higher than that in non-coronary heart disease group (29.8% vs.11.9%, P<0. 01). The incidence of coronary artery disease was associated with age, hypertension,diabetes mellitus, hypercholesterolemia and depression. Life events and stage of heart function occurred differently between CAD patients with and without depression (P<0. 05). There were no differences in the degree of coronary artery stenosis and the type of treatment (P>0. 05) between the two groups. There were higher concentration of MCP-1 in coronary artery disease patients with depression, but no remarkable difference in hsCRP and TNFa.Conclusions There is high prevalence of depressive disorder, which is one of risk factor for coronary artery disease. The depressive disorder may be associated with inflammation biomarker in patients with coronary artery disease.

BACKGROUND: Clinical experiments indicates that Yufeng capsule (composed of milkvetch root, angelica, szechwan lovage rhizome, senega and salvia miltiorrhiza) is effective for benefiting vital energy, circulating blood flow,dispelling gores and killing inflammation. The mechanism may be associated with the function of platelet aggregation, changes of contents of thromboxane B (TXB) and 6-Ketoprostaglandin F1 alpha (6-keto-PGF1 α) in plasma.OBJECTIVE: To observe the influence of Yufeng capsule on the platelet aggregation and its mechanism.DESIGN: Randomized controlled animal trial.SETTING: Department of Pathology, Daqing Branch of Harbin Medical University.MATERIALS: The experiment was performed at the Laboratory of Pharmacology, Daqing Branch of Harbin Medical University from May 2000 to July 2004. A total of 40 Wistar male gerontic rats with mean age of (8-10) weeks and mean body mass of (550±150) g and 8 Wistar male young rats with mean age of 4-6 weeks and mean body mass of (230±120) g were selected.METHODS: The 8 Wistar male youth rats were selected as youth control group. The 40 Wistar male gerontic rats were selected as the blood stasis model, and according to the numeration table method they were classified into 5 groups: aspirin group, low-dose Yufeng capsule group, the middle-dose Yufeng capsule group, high-dose Yufeng capsule group and gerontic model group, with 8 rats in each group. The Yufeng capsule was constituted by milkvetch root, angelica, szechwan lovage rhizome, senega and salvia miltiorrhiza, and provided by the Preparation Office, Second Affiliated Hospital,Heilongjiang University of Chinese Medicine. Respectively the rats were injected with aspirin solution (5.5 g/kg), Yufeng capsule (0.33 g/kg, 0.66 g/kg and 1.32 g/kg), successively for 22 days by gastric perfusion, twice a day.Those in the gerontic group and youth control group were not treated with any drug One hour after last time administration, function of platelet aggregation(PAG), contents of thromboxane B2 (TXB2) and 6-Ketoprostaglandin F1 alpha (6-keto-PGF1 α) in plasma were examined with platelet aggregation method and radioimmunoassay.MAIN OUTCOME MEASURES: Function of platelet aggregation; contents of TXB2 and 6-keto-PGF1 α as well as ratio of TXB2 to 6-keto-PGF1α.RESULTS: Totally 48 animals were involved in the result analysis. ①The PAG was higher in the gerontic model group than other groups (P ＜ 0.01).②One minute platelet aggregation rate (PAG1), five minutes platelet aggregation rate (PAG5)and the maximal platelet aggregation rate (PAGM) in the high-dose Yufeng capsule group were lower than those in the aspirin group[(7.96±2.17), (6.38±1.66), (14.33±2.87) ng/L and (31.95±3.94),(35.29±6.54), (38.68±7.46) ng/L, respectively(P ＜ 0.01 )]. PAG5 in the middle-dose group was lower than that in the aspirin group [(13.96±1.66),(35.29±6.54) ng/L, (P ＜ 0.01 )]. ③TXB2 content in the low-dose Yufengcapsule group, middle-dose Yufeng capsule group, high-dose Yufeng capsule group, aspirin group and youth control group was lower than that in the gerontic model group (P ＜ 0.01). ④The TXB2 content in the high-dose Yufeng capsule group was lower than that in the low-dose Yufeng capsule group [( 100.13±8.90), (127.00±20.99) ng/L, (P ＜ 0.01 )]. ⑤TXB2/6-ke-to-PGF1 α in the high-dose Yufeng capsule group, middle-dose Yufeng capsule group and youth control group was lower than that in the gerontic model group [0.62±0.17,0.69±0.14,1.75±0.17,1.11±1.12, (P ＜ 0.05 )].CONCLUSION: High-dose and middle-dose Yufeng capsule is able to decrease the content of TXB2 and increase the content of 6-keto-PGF1 α, and improve their ratio. It is important to enhance the balance of TXB2 and 6-keto-PGF1 α. The effect in the low-dose Yufeng capsule group is lower as compared with that in the high-dose and middle-dose Yufeng capsule groups. It is thought that the low-dose Yufeng capsule has preventive effect.

The volume of blood serum originally required for the estimation of alkaline phosphatase by Boclanaky's method has been reduced from 1.0 ml to 0.02 ml. The results obtained with this micro method were in close agreement with those obtained from the original macro procedure. Using this improved method the activity of the serum alkaline phosphatase of 144 apparently normal infants aged from 1-0 months were determined, giving an average value of .11.3 Bodansky units per 100 ml serum which is slightly higher than those found in the literature.

Objective By measuring the levels of serum MCP-1,sICAM-1 and expression of monocyte CD11b and CD62p in order to investigate the effects of these inflammation mediators on acute coronary syndrome and the relationship between inflammatory mediators and degree of coronary artery stenosis. Methods By means of enzyme-linked immunosorbent assay(ELISA) technique and flow cytometry technique,the changes of concentration of MCP-1,sICAM-1 and expression of CD11b and CD62p were measured in patients with acute coronary syndrome(ACS,n=33) group,stable angina(SA,n=24) group and control group(n=20)in the study. Results (1) The concentration of MCP-1 and sICAM-1 and expression of CD11b and CD62p had significant difference in statistics among three groups.Four inflammatory mediators were the highest in ACS group and the lowest in control group.(2)There were no significant differences in the levels of MCP-1,sICAM-1,CD11b,CD62p between AMI group and UA group.(3)There were no significant differences in the degree of coronary artery stenosis amomg SA,UA and AMI groups. Conclusions The results of the present study suggest that inflammation might play a role in the pathogenesis of coronary artery disease.

By using the diagnosis related groups(DRGs) method, we analyze and compare the rationality and feasibility of outcomes and human resources distribution of clinical specialties and health care professionals.It may increase the hospital management levels and feasibility.The introduction of DRGs for the majority of hospital management may enhance the performance evaluation and human resource levels in the hospitals.

Objective To observe the expression of acid-sensing ion channel la (ASICla) and to investigate the effect of intracellular Ca2 + concentration on focal cerebral ischemia in diabetic rats.Methods 108 male Wistar rats were divided into three groups:group A [rats with middle cerebral artery occlusion (MCAO)],group B [rats with MCAO and diabetes (DM + MCAO)],group C [rats with MCAO and diabetes treated with fasudil intervention (DM+ MCAO+ fasudil)] (n=36 each).Samples were obtained at the time points of 1,3,6 and 24 h after ischemia respectively (n=9).Models of MCAO and DM+MCAO were prepared.Rats in DM+MCAO+Fasudil group were treated with fasudil 1 mg/Kg by caudal vein injection after half an hour when DM+MCAO model successfully prepared.ASICla expressions were detected at different time points of ischemia in the 3 groups respectively.Ca2+ concentration in ischemia cortex cells were determined at different time points of ischemia in group B and C.Results ASICla expressions were gradually increased along with the ischemia time in group A and B (group A:0.71±0.10,0.80±0.11,0.86±0.08,0.93±0.09;groupB:0.86±0.11,1.05±0.51,2.42±0.08,2.78±0.04; all P＜ 0.05),and ASICla expressions at different time points were higher in group B than in group A (all P＜ 0.05).Ca2-concentration were gradually increased along with the ischemia time in group B (106.32± 18.6,137.84±14.32,151.94± 18.38,183.61±7.96,all P＜0.05).Compared with group B,the levels of ASICla expression and calcium current were reduced in group C.Conclusions The activation of ASICla increases calcium ion flow internal pathway leading to intracellular calcium overload,which may be one of the reasons for the aggravation of focal cerebral ischemia in diabetes.

Objective To investigate the changes of serum bilirubin level in elderly patients with acute cerebral infarction and its significance.Methods 164 hospitalized elderly patients,who suffered from acute cerebral infarction within 1 week after onset,were divided into 2 groups according to age:group A aged over 60 years(n=85) and group B aged 40-60 years(n=79),and 66 healthy subjects aged over 40 years were collected as controls(group C).Serum bilirubin levels in all subjects were determined.The ratio of pulse pressure over mean arterial pressure(PP/MAP) in group A and B was calculated.Nerve function scores in the three groups were detected before and after 2 weeks of treatment.Meanwhile,the data of risk factors including blood glucose,blood pressure,blood lipids,smoking and drinking in group A and B were collected.Results Compared with group C,serum total,direct,indirect bilirubin levels were increased in group A and B(both P＜0.01),and the change was smaller in group A than in group B(P＜0.05).The nerve function scores was lower in group A than in group B before and after treatment [(35.2±12.6) vs.(44.3±7.9),(40.7±9.1) vs.(51.3± 4.1),t=5.58,9.73,both P＜0.01],but PP/MAP and the numbers of risk factors were higher in group A than in group B [(0.46±0.06) vs.(0.38±0.06),93.01 vs.71.20,both P＜0.01].There were no significant correlations of serum total,direct and indirect bilirubin levels with nerve function scores in group A or B(all P＞0.05).Conclusions Serum bilirubin level is increased in patients with acute cerebral infarction,but the endogenous antioxidant capacity is decreased because of aging,multiple risk factors and more serious atherosclerosis in elderly patients,and the increment of bilirubin level is relatively smaller in acute cerebral ischemia,leading to the reduced protective effect against stress.Serum bilirubin level may influence the prognosis in patients with acute cerebral infarction.

Objective To investigate the clinical features of osteonecrosis of the knee (ONK) in patients with systemic lupus erythematosus (SLE).Methods A retrospective chart review was conducted to identify patients of SLE with ONK.The clinical characteristics of these patients were analyzed.Results Between 2008 and 2013,eighty-one patients with SLE were analyzed in the 210th Hospital of People's Liberation Army.Eleven of these patients were diagnosed as ONK.All of these ONK patients were female.The age of onset ranged from 19-57 (36±13) years.The disease duration ranged from 4.5-31.0 (13±6) years.The local clinical symptom included knee swelling with pain in 5 cases,only pain with no swelling in 1 patient,no symptoms in 5 patients.All patients were with aseptic necrosis of the femoral head.For laboratory examination,7 patients had elevated triglyceride lipids in blood.Three of 7 patients accompanied with cholesterol level increased.Total cumulative dosage of oral corticosteroids was (66 824±27 328) mg.Conclusion ONK in patients of SLE is common and the majority of them have impaired renal function and elevated blood lipids,as well as long-term glucocortico-steroid therapy.

Objective To assess the clinical value of fetal chromosomal aneuploidy diagnosed by free fetal DNA in maternal plasma in 11-13+6 gestational weeks.Methods A total of 2 650 pregnant women who had prenatal care in Tianjin Center Hospital of Obstetrics and Gynecology from January 1,2010 to December 31,2010 were included.Each of them had an ultrasound scan to measure fetal nuchal translucency thickness.Maternal serum free β-human chorionic gonadotropin and pregnancy-associated plasma protein A test was performed as part of screening for chromosomal abnormalities.Results of ultrasound and maternal plasma biochemical analysis were entered into the database,and converted into multiple of median (MoM) by factors such as maternal age,weight,ethnicity,smoking history and mode of conception.The cutoff value was 1 ∶ 270.Meanwhile,20 cases had cell free fetal DNA (cffDNA) test and the ratio of the single nucleotide polymorphism on two alleles of plancenta-specific 4 (PLAC4) were measured in 16 cases.T-test,rank sum test,MannWhitney U test and Chi-square test were used as statistical methods.Results (1) A total of 74 cases were judged as high-risk,among which 35 cases underwent transabdominal chorionic villus sampling (18 cases had cffDNA test),37 cases underwent amniocentesis at the week of 20,and two cases of Rh negative did not receive the invasive examination.Totally 20 cases,including two Rh negative cases,had the cell-free fetal DNA test.(2) By cffDNA test of maternal plasma,two cases of 21 trisomy,one case of 18 trisomy,two cases of 45,XO and one case of balanced translocation were diagnosed.(3) In the two cases of 21 trisomy,maternal plasma G/A ratio ofPL4C4 RNA-single nucleotide polymorphism alleles was 1.00 (0.98,1.02) ; in 14 pregnancies with normal chromosome,the ratio was 1.055 (1.02,1.13,Z=3.5).There was no significant difference (P=0.066).Conclusion Diagnosing of fetal chromosomal aneuploidy by cffDNA in maternal plasma is feasible and noninvasive with high negative predictive value,and can be used in Rh-negative pregnant women for prenatal screening and diagnosis.