1.Study on modulation for immunosupperssion by WWOX in Lewis lung cancer cells
Wei YANG ; Hongyan YUAN ; Yaping CHANG
Chinese Journal of Immunology 1985;0(02):-
Objective:To investigate the mechanism of WWOX regulating immunosuppression in Lewis lung cancer cells.Methods:Transfected pCDNA4.0/myc-WWOX plasmid was transfected into Lewis lung cancer cells to detected the effect of cell supernatant on generation of lymphocytes by MTT method,and mRNA expression of immunosuppression factors by semi-quantitative RT-PCR.Function change of NK,CTL to kill tumor and lymphocte proliferation were analyzed by MTT and tumor volume.Results:The suppression of the supernatant to generation of lymphocytes was present compared with control group(P
2.Chronic wound:pathogenesis and current treatments
Fei CHANG ; Changwei YANG ; Wei LU
Academic Journal of Second Military Medical University 2000;0(11):-
Chronic wound has very complicated pathogenesis and is difficult to treat;the situation has been improving owing to the progress in wound healing research and the clinical application of new techniques.This article focuses on the pathogenesis of chronic wound and introduces progress in various kinds of treatment,such as skin substitute,growth factors,negative pressure treatment,etc.
3.Effects of berberine on Insig-2 and VDR gene expression of HepG2 cell line
Wei CHANG ; Qixin ZHOU ; Junxia YANG
Chinese Traditional Patent Medicine 1992;0(07):-
AIM: To observe the mRNA expressions of Insulin-induced gene 2(Insig-2) and Vitamin D receptor(VDR) in HepG2 cell line and investigate the possible mechanism of berberine on regulating blood lipid. METHODS: HepG2 cells were incubated with Bet at different concentrations(1,3,10,30,100 ?mol/L),and incubated with 3 ?mol/L berberine for various times(0,12,24,48,72 h).Total RNA was extracted and the mRNA expressions of Insig-2 and VDR from HepG2 cells treated by berberine were quantified by RT-PCR.(RESULTS): After exposure to gradient concentrations of berberine for 24 h,the mRNA expressions of Insig-2 and VDR in HepG2 cells increased obviously peaked at 3 ?mol/L concentration,then the up-regulating effect declined gradually.The relative coefficient between them was(0.753)(P
4.Construction of recombinant adenovirus vector expressing anti-VEGF hairpin ribozyme and human interleukin-24 and its cytotoxic effect on colon cancer HT-29 cells.
Shu-Jian CHANG ; Ji-Cheng YANG ; Wei-Chang CHEN
Chinese Journal of Oncology 2009;31(6):433-433
Adenoviridae
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genetics
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Cell Death
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Genetic Vectors
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HT29 Cells
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Humans
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Interleukins
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biosynthesis
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genetics
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RNA, Catalytic
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biosynthesis
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genetics
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RNA, Messenger
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metabolism
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Recombinant Proteins
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biosynthesis
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genetics
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Transfection
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Vascular Endothelial Growth Factor A
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metabolism
5.Specific role of synovial macrophages in rheumatoid arthritis
YANG XUE-ZHI ; CHANG YAN ; WEI WEI
Chinese Journal of Pharmacology and Toxicology 2017;31(10):1026-1026
Rheumatoid arthritis (RA) is an autoimmune disease, which is characterized by synovial inflammation. Hyperplasia sublining macrophages found in synovium is an early hallmark of RA and effective treatment results in their diminution. However, the origin of these sublining macrophages in synovium (including infiltrated macrophages and tissue-resident macrophages) are still unknown both in animal models of arthritis and RA patients, let alone the differences and feature of these macro?phages. In rheumatic synovium, macrophages are submitted to a large variety of micro-environmental signals which influence the phenotypic polarization and activation of macrophages. Understanding the mechanisms and functional consequences of the heterogeneous macrophages will contribute to confirm their potential role in synovial inflammation development. Furthermore, research on macrophage plasticity to soft-control their phenotypic polarization could lead to novel therapeutic approaches.
6.Review and Outlook of the Developing Path of Bioethics
Wei YANG ; Ping DU ; Yunli CHANG ; Fang YANG
Chinese Medical Ethics 1995;0(02):-
As a young discipline imported from the West,Chinese bioethics has made tremendous achievements in the last three decades.However,at the same time,there are also certain confusions about its ideaistic origins and academic development.Observing the trends in the growth of western bioethics and the changes in the contemporary real social life,we can see that it is the time to break the current bottleneck for the contemporary development of Chinese bioethics.Specifically,Chinese bioethics should shift its attention to the quality of life,and manifests its thought depth and academic value through reflecting the reality.Advancing with the times,the bioethics is bound to have a brighter future.
7.Effects of Bone Marrow Mesenchymal Stem Cells Transplantation on Expression of Growth Associated Protein-43 in Rats after Spinal Cord Injury
Bingkui LI ; Bin ZENG ; Wei CHANG ; Dayi WANG ; Qi YANG
Chinese Journal of Rehabilitation Theory and Practice 2015;(12):1391-1396
Objective To observe the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the expression of growth associated protein-43 (GAP-43) in rats after spinal cord injury (SCI). Methods The SCI models were made by impacting at the level of T11 segment. After modeling, the rats were randomly and equally divided into control group (n=18) and observation group (n=18). 1 week after injury, DMEM/F12 medium 0.1 ml was injected into tail vein of rats in the control group, and an equal volume of BMSCs suspension was injected in the observation group. 1, 2, 4, 8 weeks after SCI, the hindlimb function was evaluated by the Basso-Beattie-Bresnahan (BBB) score. 2, 4, 8 weeks after SCI, the expression of GAP-43 mRNA was detected by RT-PCR, and the expression of GAP-43 was detect-ed by the immunohistochemistry. Results 2, 4, 8 weeks after SCI, compared with the control group, The BBB score increased, the expres-sion of GAP-43 mRNA and GAP-43 increased in the observation group (P<0.05). Conclusion BMSCs transplantation can improve the ex-pression of GAP-43 in gene and protein level, and then promote the repair of SCI.
8.The study of malignant transformation of NIH3T3 cells induced by transfection of HMGA1 gene
Xin ZHANG ; Mingguang ZHU ; Hongyan YUAN ; Wei YANG ; Yaping CHANG
Chinese Journal of Immunology 1985;0(05):-
Objective:To establish a mouse fibroblastic cell line stably transfected with HMGA1 gene,and to use the cell line to investigate the role of HMGA1 in tumor development and progression.Methods:Eukaryotic expression vector pcDNA3.0-HMGA1 was transfected into NIH3T3 by lipofectamine-mediation.Stable transfectants were selected by G418.The expression of extraneous HMGA1 gene was analyzed by RT-PCR and DNA sequencing.Cell growth was measured through MTT and the cell cycle by FCM.Soft agar colony formation was employed to analyze the ability of anchorage-independent growth.The expression of the immune inhibition factors of VEGF and FasL mRNA was detected by RT-PCR.Results:NIH3T3 cell lines stably expressing HMGA1 gene were successfully established.Comparing with controls,the HMGA1 gene-transfected cells grew faster,acquired the ability of anchorage-independent growth and expressed the immune inhibition factors of VEGF and FasL mRNA.Conclusion:Extraneous expression of HMGA1 gene can induce malignant transformation of NIH3T3 and modulate mRNA expression of immune inhibition factors.HMGA1 gene plays a key role in tumor development,progression and immune escape.
9.Bibliometric analysis about nurses′ on-the-job training evaluation in China
Guihua YANG ; Linlin JIAO ; Zongxia CHANG ; Wei YUAN ; Juan DU
Chinese Journal of Practical Nursing 2016;32(21):1665-1668
Objective By reviewing the relevant literature, we explored the current status and problems of the evaluation of the training effectiveness, and put forward the corresponding countermeasures, in order to provide a theoretical basis for the effect evaluation of nurse training in China. Methods By searching Citation online search and integration system (CITE), the Chinese biomedical literature service system (CBM), we included the literatures on the effect evaluation of the nurses training in the core journals from January 2006 to November 2015. Retrieval literatures were imported the bibliographic management software Note express by database. The corresponding field were analyzed in the literature′s age distribution, evaluation object, evaluation model, evaluation method, evaluation index and evaluation tool, et al. Results There were 343 literatures had retrieved, the total number of literatures presented a increasing tendency. The evaluation methods, evaluation tools and evaluation indicators presented a tendency of diversity, and the evaluation model was relatively single. Only 16 (4.7%) literatures tried to construct the training effect evaluation system of nurses, which was not based on some theory or hypothesis, which had not been effectively put into practice. Conclusion The effect evaluation system of nurse training needed to be done in depth, such as reconstruction of the new training evaluation model, the establishment of training evaluation system of different professional, different levels, different positions, to research evaluation methods, evaluation criteria and clinical practice testing, to design, training evaluation management software etc.
10.Clinical Observation of Chronic Pharyngitis Treated with Acupuncture
Wei GU ; Baoyue BI ; Xiaoxian JIN ; Yu CHANG ; Yang KONG
Journal of Guangzhou University of Traditional Chinese Medicine 2016;(1):38-42
Objective To compare the clinical efficacy of acupuncture and medicine for the treatment of chronic pharyngitis. Methods Sixty chronic pharyngitis patients were randomized into treatment group and control group, 30 cases in each group. The treatment group was treated with acupuncture mainly on acupoints of Zhaohai(KI6), Lieque(L7), Tiantu(CV22) , Lianquan(CV23), Tianrong(SI17) , Hegu(LI), Yuji (LU10) for nourishing yin to reduce fire and clearing throat. The supplementary acupoints were selected according to the symptoms and physical signs. Acupuncture was performed once a day and 6 times a week. The control group received western medical therapy including pharyngeal application with 1% iodine glycerin preparation and oral use of Cydiodine tablets, 3 times per day. Four weeks constituted one treatment course and the treatment for the two groups covered 2 courses. After treatment, the therapeutic effect was evaluated. The scores of the signs and s ymptoms as well as scores of chronic pharyngitis syndrome discomfort rating questionnaire(CPSDQ) evaluated by visual anal og scale(VAS) were observed. Results (1) The overall effective rate of the treatment group was 93.3%, higher than that of the control group(73.3%), and the inter-group difference was statistically significant (P<0.05). (2) After treatment, scores of the signs and symptoms and VAS for CPSDQ scores in the two groups were reduced as compared with those before treatment(all P<0.05 or P<0.01 ) .The improvement of the scores in the treatment group was superior to that in the control group(all P<0.05 or P<0.01).(3) During the treatment , needling-induced mild pain and bleeding occurred in the treatment group. No acupuncture syncope, stuck needles or allergic and toxic-side effect was shown in the two groups. Conclusion Acupuncture therapy can obviously relieve the symptoms, signs and discomfort in the patients with chronic pharyngitis, and the curative effect is superior to the drugs.