Objective To explore effects of two intravenous infusion methods on elderly patients.Methods Self-control method was used on 568 cases of elderly patients.Traditional method was used on Day 1:The adjustable clamp was fully opened when a free flow of blood appeared during venipuncture.The experimental method was used on Day 2:The adjustable clamp was opened 1/3 when a free flow of blood appeared during venipuncture.Success rate of venipuncture and value of pain were compared between the two groups.Results The experimental method had a higher success rate of venipuncture than the tranditional method (97.89％ vs 93.31％,x2 =13.098,P ＜ 0.01 ).There was no significant difference in the mean value of pain during a successful venipuncture ( P ＞ 0.05 ).During an unsuccessful venipuncture,mean value of pain in the experimental group was lower than that in the traditional method group,and the difference was statistically significant [ ( 1.97 ±0.52) vs (2.35 ± 0.66 ),t =4.987,P ＜ 0.01 ].Conclusions Opening the adjustable clamp for 1/3 when a free flow of blood appeared during venipuncture on elderly patients could improve the success rate of venipuncture and decrease pain during an unsuccessful venipuncture.

OBJECTIVETo investigate the adverse effects of acupuncture on the prognosis, and effectiveness of acupuncture combined with far infrared ray in the patient of acute Bell's facial paralysis within 48 h.

METHODSClinically randomized controlled trial was used, and the patients were divided into 3 groups: group A (early acupuncture group), group B (acupuncture combined with far infrared ray) and group C (acupuncture after 7 days). The facial nerve functional classification at the attack, 7 days after the attack and after treatment, the clinically cured rate of following-up of 6 months, and the average cured time, the cured time of complete facial paralysis were observed in the 3 groups.

RESULTSThere were no significant differences among the 3 groups in the facial nerve functional classification 7 days after the attack, the clinically cured rate of following-up of 6 months and the average cured time (P > 0.05), but the cured time of complete facial paralysis in the group A and the group B were shorter than that in the group C (P < 0.05).

CONCLUSIONThe patient of acute Bell's facial paralysis can be treated with acupuncture and moxibustion, and traditional moxibustion can be replaced by far infrared way.

Acupuncture Therapy ; Bell Palsy ; therapy ; Facial Paralysis ; therapy ; Humans ; Moxibustion ; Treatment Outcome

OBJECTIVETo compare clinically therapeutic effects of warming needle moxibustion and simple acupuncture on knee osteoarthritis.

METHODSOne hundred and twenty cases conformed with the diagnostic criteria of knee osteoarthritis with TCM kidney-yang deficiency and cold syndrome were randomly divided into a warming needle moxibustion group and a simple acupuncture group. Guanyuan (CV 4), Qihai (CV 6), Zusanli (ST 36) and others were selected in the two groups. Their therapeutic effects were assessed with joint pain scale, knee osteoarthritis severity index scale and cold syndrome differentiation factors.

RESULTSBoth warming needle moxibustion and simple acupuncture could alleviate pain of knee osteoarthritis, improve dysfunction of the knee joint, reduce symptoms. The clinically basic cured rate and the total effective rate were 32.1% and 96.4% in the warming needle moxibustion group, which were better than 7.4% and 92.6% in the simple acupuncture group.

CONCLUSIONWarming needle moxibustion is an effective therapy for knee osteoarthritis with kidney-yang deficiency and cold type.

Acupuncture Therapy ; Humans ; Moxibustion ; Needles ; Osteoarthritis, Knee ; therapy ; Yang Deficiency

Objective To explore the application of modified seldinger technique (MST) in advanced cancer patients and its superiority.Methods The MST was used in 20 patients who required peripherally insert central catheter (PICC) and they were divided into experimental group (n=40) and control group (n=40). The experimental group was treated with seldinger technique and the control group was treated with traditional PICC. The intubation success rate, complication rate and clinical application advantages were accounted and compare between the two groups.Results The intubation success rate in experimental group was 97.5% and that in control group was 80.0%,there were statistical significance (χ2=6.135,P<0.05); the incience of postoperative complications between experimetal group and control group were statistical significance (χ2=12.468,16.970,2.051;P<0.05).Conclusions Modified Seldinger technique with ultrasound guided have a useful effect, it should be widely carried out in clinical.

OBJECTIVETo observe the therapeutic effect of acupuncture at five mental points and moving cupping on the Hechelu of the back on fibromyalgia syndrome (FS).

METHODSSixty-six cases who conformed to the criteria were randomly divided into the treatment group treated with acupuncture at five mental points, moving cupping on the Hechelu of the back and amitriptyline, and the control group treated with amitriptyline. Clinical therapeutic effects were assessed with McGill Pain Questionnaire (MPQ) and HAMD depression scale.

RESULTSThe therapeutic effect of the treatment group was better than that of the control group with a significant difference between the two groups (P < 0.01).

CONCLUSIONCombination of acupuncture with cupping therapy is an effective therapy for fibromyalgia syndrome.

Acupuncture Points ; Acupuncture Therapy ; Biomedical Research ; Fibromyalgia ; Humans ; Pain Measurement

OBJECTIVETo observe the curative effect of AMIE methods on movement disorder in the child of convulsive cerebral palsy (CP).

METHODSOne hundred and twenty cases of CP children were randomly divided into an integration treatment group and a rehabilitation group, 60 cases in each group. The integration treatment group were treated with acupuncture (A), massage (M), injection (I) and five animal exercise (E) therapies for 60 times, and the rehabilitation group were treated with Bobath training therapy for 60 times. Scores for movement function before and after treatment were used for assessment of therapeutic effect. Twelve months later, understand whether or not the children can walk on ones own.

RESULTSThe total effective rate was 76.70% in the integration treatment group and 58.4% in the rehabilitation group, with no significant difference between the two groups (P > 0.05); there were significant differences in the two groups in differences of movement function scores before and after treatment (P < 0.05). After one year's follow-up survey, 36 children could walk by themselves in the integration treatment group, which were significantly more than 24 children in the rehabilitation group (P < 0.05).

CONCLUSIONAMIE methods is effective for treatment of movement disorder in the child of convulsive cerebral palsy, and the short-term therapeutic effect is same as that of Bobath training method and the long-term therapeutic effect is better than that of Bobath training method.

Acupuncture Points ; Acupuncture Therapy ; methods ; Cerebral Palsy ; therapy ; Child, Preschool ; Combined Modality Therapy ; Female ; Humans ; Infant ; Injections ; Male ; Massage ; Medicine, Chinese Traditional ; Movement Disorders ; therapy

Objective To investigate the current situation of medical instrument cleaning in Central Sterile Supply Departments(CSSD),in order to identify problems and provide references for further improvements of equipment cleaning.Methods Questionnaire survey was adopted,data of 127 CSSDs in five coastal provinces or cities in China in 2017 were collected,and status of each link of medical instrument cleaning was analyzed.Results The allocation rate of ultrasonic cleaner and spray cleaner were 96.6％ and 78.7％,respectively;62.2％ of hospitals used tap water to pre-rinse,and 10.2％ used normal saline or sterile water to pre-rinse,11.0％ used tap water for finial rinsing;78.7％ of hospitals did not perform continuous humidification in transit,and 18.1％ of hospitals did not perform continuous humidification and their time of transit was more than 2 hours;66.9％ of CSSDs only used multi-enzyme detergent.More than half of the hospitals did not record time for manual rinsing/manual final rinsing.Conclusion The configuration of medical instrument cleaning equipment of 127 CSSDs is good.However,it is necessary to pay more attention to cleaning details.Specifications for instrument cleaning water should be established;continuous humidification during transportation of medical instruments should be promoted;cleaning methods and cleaning detergents should be selected reasonably;standardized rinsing should be enhanced to ensure quality of medical instrument cleaning.

Background:Reduced expression of tripartite motif-containing 3 (TRIM3) has been reported to be involved in the pathogenesis of human glioblastoma.In our previous research,we found that TRIM3 expression was markedly reduced in human primary hepatocellular carcinoma (HCC) tissues and that low TRIM3 expression was associated with short survival of HCC patients.However,the role of TRIM3 in liver cancer remains unknown.This study aimed to investigate the function of TRIM3 in liver cancer cells.Methods:The protein levels of TRIM3 in five liver cancer cell lines (SK-Hep1,Hep3B,Huh7,HepG2,Bel-7402) and one normal liver cell line (L02) were detected with Western blotting.HepG2 and Bel-7402 cells with IowTRIM3 expression were infected with recombinant lentiviruses overexpressing TRIM3 (LV-TRIM3),whereas Huh7 and Hep3B cells with high TRIM3 expression were transfected with TRIM3-targeted small interfering RNA (siTRIM3).The functions of TRIM3 in the proliferation,colony formation,cell cycle,migration,invasion,and apoptosis of the above cell lines were examined.The effect of TRIM3 on tumor growth and metastases in nude mice was also investigated.Results:TRIM3 was overexpressed in HepG2 and Bel-7402 cells with LV-TRIM3 infection,which further reduced proliferation,colony formation,migration,and invasion of both cell lines.Cell cycle analysis showed thatTRIM3 overexpression induced G0/G1 phase arrest in HepG2 and Bel-7402 cells.Moreover,apoptosis was not increased in HepG2 or Bel-7402 cells overexpressing TRIM3.Contrarily,silencing TRIM3 expression in Huh7 and Hep3B cells by siTRIM3 led to significantly decreased percentages of both cells in the G0/G1 phase and promoted cell proliferation,colony formation,migration,and invasion.In vivo experiment results confirmed thatTRIM3 overexpression suppressed tumor growth and metastasis.Conclusions:TRIM3 plays a tumor-suppressing role in the regulation of liver cancer development by reducing cell proliferation through cell cycle arrest at the G0/G1 phase.

Objective To evaluate the effect of erythropoietin (EPO) on cognitive function in rats with traumatic brain injury (TBI) and investigate its mechanisms. Methods Forty-eight SD rats were equally randomized into control, sham-operated, TBI and EPO+TBI groups. TBI models were established in the later 2 groups by operation and hydraulic shock. Sham-operated group received the operation only. The EPO+TBI group was injected with EPO immediately after the success of model making; the other groups were injected with saline at the same time. On the 30th d of injury, Morris water maze was employed to evaluate the cognitive function of the rats and the expression of brain-derived growth factor (BDNF) was detected by immunohistochemistry. Results In the navigation experiment, the latent period (the rats on founding the platform) in the control and sham-operated groups was significantly shorter than that in the other 2 groups (P<0.05); that in the TBI group was statistically longer than that in the EPO+TBI group (P<0.05). In the space searching experiment, the swimming times of the rats in each quadrant were significantly different (P<0.05): the control and sham-operated groups were the longest and the TBI group was the shortest of all the 4 groups. Immunohistochemistry showed that the expression of BDNF in the EPO+TBI group was significantly higher than that in the other 3 groups (P< 0.05). Conclusion TBI can damage cognitive function of the rats, while exogenous EPO may improve their memory abilities by up-regulating the expression of BDNF.

Objective:To develop an Oral Frailty Assessment Scale for Elderly Inpatients and test its reliability and validity.Methods:Through literature review, qualitative interview, expert consultations and pre-investigation, the preliminary draft of Oral Frailty Assessment Scale for Elderly Inpatients was formed. Using the convenient sampling method, a total of 300 elderly patients who were hospitalized in Geriatrics Department of Zhongnan Hospital of Wuhan University from April to June 2023 were selected as the research subjects. The reliability and validity of the scale were tested using item analysis, exploratory factor analysis, confirmatory factor analysis and reliability analysis.Results:A total of 300 questionnaires were distributed in this study, and 274 valid questionnaires were collected, with an effective response rate of 91.33% (274/300). The Oral Frailty Assessment Scale for Elderly Inpatients included four dimensions of teeth, dry mouth, swallowing and chewing, with a total of 11 items. The exploratory factor analysis extracted four common factors, and the cumulative variance contribution rate was 69.059%. The Cronbach's α coefficient for the scale was 0.76, the split-half reliability coefficient was 0.67, and the retest reliability coefficient was 0.98.Conclusions:The Oral Frailty Assessment Scale for Elderly Inpatients has good reliability and validity, which is suitable for evaluating oral frailty in elderly inpatients.