Tumor markers for early diagnosis of cancer is an important auxiliary mean,which for early tumor detection,monitoring and for judging curative effect of treatment regimens play important role.Clinical method for early diagnosis on ovarian cancer is the detection of serum CA125,however,its precision and specificity for early diagnosis are not enough.It has been found that in the development period of the patients with ovarian cancer,SMRP and CA125 is co-expression.This article reviews the value of serum mesothelin on diagnosis of epithelial ovarian cancer to provide reference on early diagnosis and treatment.

Objective To study the diagnostic value of spiral CT pulmonary angiography(SCTPA) for pulmonary embolism(PE).Methods SCTPA scans were performed in 25 patients with PE,the contrast-enhanced SCT scans of the pulmonary arteries were performed,MPR,MIP and SSD were performed simultaneously.Results The 29 SCTPA scans were performed in 25 patients, including the central type in 23 cases(92%) and the peripheral type in 2 cases(8%).The rates of the main,the sinistral and dextral,the middle lobular,the lobular and the lingular pulmonary artery displayed were 100%,the rates of the segmental pulmonary artery displayed were 97.77% and the rates of the subsegmental pulmonary artery displayed were 68.00%.The lobular and segmental pulmonary artery were mainly embolized,the rates of PE were between 46.58% and 75.00%.Conclusion SCTPA has great value in earlier and clear and definite diagnosis of PE.

To explore the mechanism of platelet activateation in vascular dementia(VD), the activity of myosin light chain phosphorylation and Ca 2+ Mg 2+ ATPase,the concentration of [Ca 2+ ]i were measured in 28 healthy controls, 38 patients with acute cerebral infarction (ACI), and 32 patients with VD. The activity of MLCK in VD and ACI groups was significantly higher than that in the controls( P

BACKGROUND: The method of culture purified sinoatrial node cell is important in investigating its ultrastructural characteristics and autorhythmic mechanisms.However,the corresponding method has not been standardized.OBJECTIVE: To summarize the localization,cultivation and purification of sinoatrial nodes isolated from newborn rabbits,and to study the morphological characters of primary cultured pacemaker cells.METHODS: Hearts of the newborn rabbits(within 24 hours)were embedded in paraffin for hematoxylin-eosin staining,The location of sinoatrial nodes was observed under an optical microscope,the morphology of sinoatrial nodes cells were observed by light microscope and electron microscope.RESULTS AND CONCLUSION: Sinoatrial nodes localized in the anterior wall of the superior vena cava and the posterior laterial wall of right atrium.There was about 0.32 mm between its lowest point and sulcus terminalis.Three distinct types of cells were observed among the cultured cells of sinoatrial nodes: spindle,spider and polygon.The spindle cells occupied the greatest proportion of the cultured cells(59.6±7.3)%.The spontaneous contraction frequency of spindle cells was the highest among the constracting cells(145±9)times per minute.The ultrastructure observation showed that myofibrils and other organelles in spindle cells were sparse and significantly decreased in number compared with triangle cells.There was no significant difference between triangle cells isolated from sinoatrial nodes and from atrial muscle.Sinoatrial nodes could be harvested along the anterior root of the superior vena cava down to the posterolateral sulcus.Among the cultured cells from neonatal rabbit sinoatrial nodes,the spindle cells with small body and fast pulse frequency are pacemaker cells.

This paper discusses the current situation that care workers in hospice agencies are lack, the staffing is unreasonable, the nursing workers′education degrees are low and are old, and the present situation of the com-prehensive quality is not high. In addition, it analyzes the causes of this status and put forward the corresponding countermeasures from aspects of the medical college education, social voluntary organizations, national political sys-tem construction and hospice agencies.

Objective To elucidate the relationship between mandibular lateral displacement and mandible asymmetry by observing the morphological changes of condylar head and mandible during mandibular lateral displacement in growing rats.Methods Forty-eight male Wistar rats at the age of four weeks were divided at random into experimental and control groups,with 24 rats in each group.A super-hard resin plate was cemented to upper incisors to displace rat mandibles 2 mm to the left during closure(Ipsilateral side).A metal crown was fitted to lower incisors.The rats were killed 2,4,8 or 12 weeks after appliance attachment.The mandible was dissected out and halved.The length and width of condylar head were measured with a caliper.Radiographic films of the mandibles were exposed,and selected measurements were made.Results The length of condylar cartilage on the ipsilateral side was significantly larger than contralateral side in experimental group(P

Recently,a large family of G-protein coupled receptors (GPCRs) has been identified as mas-related genes (Mrgs),Which are specific expressed in small diameter sensory neurons in the trigeminal and dorsal root ganglia,suggesting a role in pain transmission.Mrgs receptors have been shown to modulate some physiological and pathological activities,such as pain and immunity.It is important to discover ligands of Mrgs for understanding and elucidating their potential physiological and pathophysiological roles.The studies on pharmacological spectrum of NPS make it an interesting target for pharmaceutical development.

Objective To study the influence of ion concentration change out of cells on calcium transportation and the relationship between the rising of calcium concentration in the Caco-2 cells and its apoptosis to offer the theoretical and experimental bases for clinical study and digestive tract physiology and patholoogy. Methods Confocal laser scanning microscope(CLSM) and flow cytometry(FCM) were used in this study. Results (1) Diversion of Ca 2+ into cells was increased with the decrease of Fe 3+ concentration out of Caco-2 cells(the final consistence of DFO was 100-300??mol/L),but was hindered with the increase of Fe 3+ concentration out of them(the final consistence fo FAC was 10-100??mol/L) as observed under CLSM; (2) The cell state was fine and its viability was more than 90% as observed under CLSM after treated by A 23187 and Fluo-3/AM(examined with FDA);(3) The Ca 2+ concentration in the Caco-2 cells was increased by A 23187 and this function was dose depended.The Caco-2 cell apoptosis was induced by the increase of Ca 2+ in cells,which was examined with FCM.Conclusion The Ca 2+ transportation was increased with the decrease of Fe 3+ concentration out of Caco-2 cells but was hindered with the increase of Fe 2+ concentration out of them.The Caco-2 cell apoptosis was induced by the increase of Ca 2+ concentration in them.

Objective To investigate the therapeutic effect of different doses of caffeine on premature apnea.Methods 80 patients with apnea were divided into observation group and control group,each group in 40cases.The two groups applicated caffeine citrate.The control group received the basal dose, first application of 20 mg/kg, intravenous injection, intravenous injection of 5 mg/kg after 24 h.The observation group was treated with intravenous injection of 10 mg/kg after 24 h.The treatment period was 7 d.The therapeutic effect, blood gas and oxygenation index and adverse reactions were observed in the two groups.Results The effective rate of the observation group was 92.5%,which was significantly higher than 75.0% of the control group(x2=4.501,P<0.05).After treatment, the duration[(9.05±1.82)s vs (13.53±2.63)s]and lost time[(2.52±0.94)h vs (3.42±1.13)h]of apnea in the observation group were longer than those of the control group.The number of seizures[(22.15±4.16) vs (33.53±8.48)]was less than that of the control group,the differences were statistically significant(t=8.767,5.434,9.231,all P<0.05).After treatment, PaO2[(84.74±9.74)mmHg vs (71.46±8.53)mmHg],PaCO2[(42.62±4.15)mmHg vs (48.54±5.18)mmHg]and PaO2/FiO2[(221.52±26.51)mmHg vs (197.76±21.71)mmHg]in the observation group were significantly better than those of the control group(t=7.574,4.765,8.643,all P<0.05).The incidence rate of adverse reactions include tachycardia, hyperglycemia, hypoglycemia and restlessness had no significant difference between the two groups (15.0% vs 10.0%, P>0.05).Conclusion In the treatment of premature apnea, advanced caffeine citrate treatment can better improve the clinical symptoms and blood gas index, the curative effect is reliable.

Objective To analyze risk factors for grenade throwing fractures and put forward corresponding preventive measures for the fractures during the military training in recruits,so as to reduce the happen in the military training.Methods The research is case-control study.The trial group and the control group (39 patients each) were followed up and investigated.The investigation indicators included height,body mass index (BMI),whether drinking carbonated beverage frequently,literacy,osteoporosis,throwing training score,throwing posture,warm-up sufficiently,region,whether attend often physical exercise before recruitment,exercise strength,and weather factor.Results There were significant differences in the warm-up sufficiency,attending physical exercise before recruitment,exercise intensity,throwing posture,weather factor between trial group and the control group in recruits.The logistic regression analysis showed that the lack of physical exercise before recruitment,strong exercise intensity,nonstandard throwing posture were the risk factors in grenade throwing fractures in recruits.Conclusion Sufficient warm-up,avoiding exhausted exercise and assault exercise,strict training in accordance with the standard throwing posture,regular participation in physical exercise before recruitment and training in warm season are effective methods for preventing grenade throwing fractures in recruits.