3.Value of HASTE MR Cholangiopancreatography in the Diagnosis of the Biliary Obstructive Diseases
Zhiping YAN ; Xuelin ZHANG ; Fachao ZHI ; Hong ZENG ; Renmin CHANG
Journal of Practical Radiology 1992;0(11):-
Objective The aim of this study was to evaluate magnetic resonance cholangiopancreatography(MRCP)using half-Fourier acquisition single-shot fast spin-echo(HASTE)sequence in the diagnosis of biliary obstructions.Methods Forty-five patients with pancreaticobiliary duct diseases underwent MRCP on 1.5 T scanner,a heavily T 2-weighted HASTE was used during a breath-hold.The source images were three-dimensional reconstructed postprocessed on workstation.The findings of MRCP images were analyzed and compared with US,CT,ERCP or PTC.Results The diagnostic accuracy of MRCP was 94%,the same as ERCP(92%),but it was superior to US and CT(?
4.Cognitive Function and P300 Potentials in First-Episode Schizophrenia Treated with Quetiapine and Risperidone
Chang-Hong WANG ; Yan LI ; Xu LIU ; Al ET ;
Chinese Mental Health Journal 1991;0(05):-
Objective:To compare the effects of quetiapine and risperidone on cognitive function of first-episode schizophrenic patients.Methods:A randomized comparative clinical design was applied to compare the cognitive function of 67 patients with first-episode schizophrenia treated with quetiapine(n=35,25-750mg/d)and risperidone(n=32,1-7rag/d)before and after 12-week treatment.PANSS,WMS and P300 were used to evaluate adverse effects of drags and cognitive function.Results:The Long-term memory,Short-term memory,Immediate memory,Memory Quotient of first-episode schizophrenia were damaged obviously.Latency periods of P_2、N_2 and P_3 of P_(300) potentials with first-episode schizophrenia group were longer than the control group,The amplitudes of P_2 and P_3 of P_(300) potentials with first-episode schizophrenia group were lower than the control group(P
5.Effect of blocking Wnt-?-catenin signaling pathway on activation of hepatic stellate cells
Zhi-Hong WENG ; Yan-Chang LEI ; Cheng PENG ; Shuling ZHANG ;
Chinese Journal of Infectious Diseases 2007;0(07):-
Objective To investigate whether functional Wnt-?-catenin signaling is present in activated hepatic stellate cells (HSC),and the effect of blocking this signaling on activation of HSC. Methods?-catenin expression in HSC was examined by immunocytochemistry.Wnt signalings in HSC-T6 were assessed using a T cell factor (TCF)-dependent luciferase reporter gene (pTOP- FLASH) assay.Wnt signalings in HSC-T6 were blocked by transfecting with a dominant negative TCF (dnTCF) expression plasmid,then the expression of alpha smooth muscle actin (?-SMA) and collagen typeⅠwere examined by Western blot.Results?-catenin staining was positive in the nuclei of HSC-T6.Luciferase activity in the cells transfected with pTOPFLASH was significantly higher than that in the cells transfected with pFOPFLASH (P
6.Clinical Application of Immune-related Response Criteria in Evaluating Chinese Medical Treatme for Advanced Non-small Cell Lung Cancer.
Hai-wei JIANG ; Qing HU ; Dan-feng HE ; Chang GAO ; Yan-hong YAN ; Lin-tong GE
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(9):1074-1077
OBJECTIVETo evaluate the applicability of immune-related response criteria (irRC) in treating non-small cell lung cancer (NSCLC) by Chinese medicine (CM).
METHODSTotally 97 stage III a-IV NSCLC patients were predominantly treated with comprehensive CM. Curative effects were evaluated by three methods such as Response Evaluation Criteria in Solid Tumors (RECIST), Oncologic Curative Effect Evaluation Criteria of Chinese Medicine in Solid Tumor (draft, abbreviated as CM criteria), and irRC. The correspondency and consistency between irRC, RECIST and CM criteria were analyzed and compared. The objectivity of irRC in evaluating curative effect of Chinese medical treatment for NSCLC was assessed.
RESULTSThe correspondency rate of irRC to RECIST was 59. 79% with Kappa value of 0. 379 (U test, P <0. 01). The two criteria had certain correspondence, but with an unsatisfactory consistency. The correspondency rate of irRC to CM criteria rate was 83. 51% with Kappa value of 0.751 (U test, P <0. 01). The two criteria had good correspondence and consistency.
CONCLUSIONSCM criteria had good consistency with CM criteria in evaluating curative effect for Chinese medical treatment of advanced NSCLC. Its results could objectively reflect features and advantages of CM for treating advanced NSCLC.
Asian Continental Ancestry Group ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; immunology ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Lung Neoplasms ; drug therapy ; immunology ; Medicine, Chinese Traditional ; standards ; Treatment Outcome
7.On the Reform of Experimental Teaching of Microbiology for Pharmacy Speciality
Yan GUAN ; Hui-Juan CHENG ; Chang-Zhong WANG ; Hong-Xia LE ; Yan WANG ;
Microbiology 1992;0(06):-
The authors helpfully discuss the design idea,experimental module design,examination methods,and experiment textbook construction in experimental teaching of microbiology,and conduct further researches on the basic skill training,verifying experiment,integrative experiment,and investigative experiment in the course. This study aims to enhance effects of the experimental teaching,to cultivate high potential talents who can master essential knowledge and skills,and creatively carry out scientific research.
8.Simultaneous determination of purity and potency of the components of gentamycin using high-performance liquid chromatography.
Li-Hong YANG ; Yan CHANG ; Shang-Chen YAO ; Chang-Qin HU
Acta Pharmaceutica Sinica 2012;47(12):1660-1666
The quality of some earlier developed antibiotics is usually ensured by the combination of HPLC purity and microbiological potency measurement in the pharmacopoeias of various countries because the relationship between their purity and potency is not clearly quantified. Due to potency is assessed using certain units of measurement, it can not be directly traced to the international system of units (SI unit). This has become a hotspot in the study of the quantitative relationship between purity and potency of antibiotics. It would be quite an achievement to simultaneously determine both purity and potency using HPLC methods during quality control. This study evaluated a multicomponent antibiotic product, gentamycin, as a test sample. First, pure samples of the C components of gentamycin: C1a, C2, C2a and C1 were prepared, separately. Second, quantitative relationship (theoretical potency) between the purity and potency of each C component of gentamycin were determined using 1H NMR, HPLC-ELSD and microbiological assay method. One milligram of gentamycin C1a, C2, C2a and C1 was equal to 1 286.98, 1 095.74, 1 079.52 and 739.61 gentamycin units, respectively. Finally, a method for the determination of gentamycin potency was established based on the proportion and content of C components of gentamycin. The unification of purity and potency for gentamycin was achieved using only HPLC-ELSD. It is also demonstrated that C components of gentamycin and micronomicin produce the same responses under ELSD, which means that it is not necessary to prepare separate reference standards for each C component of gentamycin and that quantitative testing can be performed accurately using only one micronomicin reference standard. This study simplified the previous method for the determination of the content of C components of gentamycin using HPLC-ELSD. The developed method is suitable for regular use as a part of quality control and can simplify the rigmarole quality control procedures provided in current pharmacopeias.
Chromatography, High Pressure Liquid
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Gentamicins
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chemistry
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pharmacology
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Magnetic Resonance Spectroscopy
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Microbial Sensitivity Tests
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methods
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Molecular Structure
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Quality Control
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Reference Standards
9.Technical improvement in dealing with chronic atrophic gastritis samples.
Hong-xi MA ; Hai-feng WANG ; Zhuang TIAN ; Chang-yan XU
Chinese Journal of Pathology 2012;41(9):637-638
Adult
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Aged
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Aged, 80 and over
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Female
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Gastritis, Atrophic
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diagnosis
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pathology
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Gastroscopy
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methods
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standards
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Humans
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Male
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Middle Aged
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Specimen Handling
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methods
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Tissue Embedding
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methods
10.Expression and significance of hypoxia-inducible factor 1alpha protein and microvessel density marked by CD105 in cervical carcinoma.
Yan ZHU ; Qi-chang YANG ; Hong-bin LIU ; Man-hua LIU ; Yi SHEN ; Xiao-juan ZHANG
Chinese Journal of Pathology 2008;37(4):264-265
Adult
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Aged
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Aged, 80 and over
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Antigens, CD
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immunology
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Endoglin
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit
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genetics
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metabolism
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Microvessels
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immunology
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Middle Aged
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Nitric Oxide Synthase Type II
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metabolism
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Receptors, Cell Surface
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immunology
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Uterine Cervical Neoplasms
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genetics
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metabolism