Objective To explore the protective effect of co-treatment with antioxidants 4-hydroxy-2,2,6,6-tetra-methylpiperidine 1-oxyl (TEMPOL) and reduced glutathione (GSH) in pancreatic injury induced by intermittent hypoxia (IH) in mice. Methods Fifty male C57BL/6J mice were randomly divided into control group(CON), IH group (IH), IH+TEM-POL group (IH+TEMPOL), IH+GSH group (IH+GSH) and IH+TEMPOL+GSH group (IH+TEMPOL+GSH). After successful-ly prepared animal model, the insulin resistance (IR) level, pancreatic malondialdehyde (MDA) level,superoxide dismutase (SOD) activity,glutathione reductase (GR) concentration and pancreatic β-cell apoptosis were detected in five groups. Re-sults The blood glucose level was significantly increased in IH group than that in CON group (P<0.01), but which was sig-nificantly decreased in IH+TEMPOL+GSH group than that of IH group (P<0.01). The MDA content was significantly higher in the IH group than that in CON group (4.48±0.25 vs 1.94±0.21, P<0.01), but SOD activity and GR content were signifi-cantly lower in IH group than those of CON group (61.52±3.33 vs 100.05± 7.26,107.81±7.54 vs 170.54±4.90,P<0.01). The level of MDA was significantly lower in IH+TEMPOL+GSH group (2.07±0.35) than that in IH group. The levels of SOD and GR were significantly higher in IH+TEMPOL+GSH group (96.68±5.85 and 166.87±5.75) than those of IH group (P<0.01). The apoptotic rate of pancreaticβcells was significantly increased in IH group than that in CON group (2.43±0.07 vs 0.54± 0.06, P<0.01), but it was significantly lower in IH+TEMPOL+GSH group (0.56 ± 0.06) than that in IH group (P<0.01). There were no significantly differences in all above indexes between IH group, IH+TEMPOL group and IH+GSH group ( P>0.05). Conclusion The co-treatment with the antioxidant TEMPOL and GSH can obviously protect IH-induced pancreatic injury in mice. However, there was no significant protective effect of TEMPOL or GSH alone on pancreatic injury.

Fatal Outcome ; Humans ; Infant, Newborn ; Leukemia ; blood ; congenital ; diagnosis ; Leukocyte Count ; Male

Animals ; Apolipoproteins ; blood ; Cattle ; Colostrum ; Esterases ; blood ; Female ; Insulin-Like Growth Factor I ; Lipids ; blood ; Nephrotic Syndrome ; blood ; Pregnancy ; Rats ; Rats, Sprague-Dawley

Bleomycin ; analogs & derivatives ; therapeutic use ; Embolization, Therapeutic ; methods ; Female ; Hemangioma ; therapy ; Humans ; Infant ; Iodized Oil ; therapeutic use ; Syndrome ; Thrombocytopenia ; therapy

Objective To investigate the efficacy and safety of patients with malignant pleural effusion treated with injecting endostar combined with platinum complexes into pleural cavity.Methods Cochrane systematic review methods were used in the data selection,and data were selected from the PubMed,Embase,Cochrane Library,China National Knowledge Infrastructure (CNKI),WanFang,and VIP database to get all clinical controlled trials.The retrieval time was August 2014.The objects of these randomized controlled trials were malignant pleural effusion patients.Endostar combination with platinum complexes was used for the experimental group.Platinum complexes alone were used for the control group.The efficacy and adverse effects of two groups were compared.The quality of included trials was evaluated by two reviewers independently.The software RevMan 5.3 was used for meta-analyses.Results Nine trials with 488 patients were included according to the including criterion.All trials were randomized controlled trials,one of them had B level in quality and eight had C level.Meta analysis results were as follows:there was significant difference in overall effective rate (OR =3.52,95％ CI =2.37 ～ 5.22),Karnofsky (KPS) score changes rate (OR =2.64,95％ CI =1.67 ～ 4.19),between endostar combination with platinum complexes and platinum complexes alone group.The incidences of severe leucopenia (OR =1.0,95％ CI =0.62 ～ 1.61) and nausea and vomiting (OR =0.77,95％ CI =0.43 ～ 1.38) were similar in the endostar combination with platinum complexes group compared to those in the platinum complexes alone group.Conclusions In the treatment of malignant pleural effusion,injecting endostar in combination with platinum complexes into pleural cavity improves the effective rate without obviously raised side effects.Owing to the small sample size and poor quality of included trials,more well-designed double-blinded randomized controlled trials should be performed.

Objective To observe the clinical curative effect of selective bronchial arterial embolization for severe hemoptysis.Methods Thirty-eight hospitalized patients with severe hemoptysis was enrolled in this study.Of which 31 patients were recurrent hemoptysis,27 patients were with bronchiectasis,4 patients were with bronchial lung cysts,4 patients had unknown causes,3 patients were with tuberculosis.All patients were diagnosed by chest imaging examination,fiber bronchoscopy,bronchial arteriography,and they were all treated by selective bronchial arterial embolization.Results The most times of embolization was 3,and bronchial arterial imaging were performed for vascular malformation.After having bronchial arterial embolization,35 patients were immediately released of bleeding.Postoperative 24 h,8 patients had hemoptysis again,of which 1 case was performed with conservative treatment and subsequently had pneumonectomy,5 patients had effective conservative treatment,2 cases were performed embolization again after failed conservative treatments.Hemoptysis completely disappeared within 1 week.The total effective rate was 97.4％(37/38).Patients were followed up for 1 year,of which 13 patients relapsed again,11 patients'annual and each time's quantity of hemoptysis reduced 50％.Two patients had pneumonectomy after a number of embolization.The total effective rate was 94.7％ (36/38).After treatment,3 patients had mild chest pain in short time,2 patients had shoulder pain,3 patients had chest tightness,and 3 patients had subcutaneous passive congestion.After being treated for short-term,these symptoms disappeared eventually.Conclusion Selective bronchial arterial embolization for the treatment of acute or recurrent severe hemoptysis is very effective,and can avoid the risk of surgery.It is effective for recurrent cases and worthy of clinical application.

Objective To study the effect of Guiji cream in the treatment of fissure and permeability of skin.Methods The models of fissure Japonic rabbits were established to observe the effects of skin cream on inhibiting fissure.Rabbits back area was divided into 6 blocks and given different medicines in six different groups:GuiJi cream group of low dosage,middle dosage and high dosage (0.05、0.1、0.2 g/m-2 crude drug of Bletilla colloid respectively),urea frost cream group(0.1 g/m-2),normal saline group and control group.Observe the changes of skin surface symptoms every day.The permeability test was performed on 36 KM mouse and randomly divided into control group,GuiJi cream group and urea frost cream group(0.2 g/m-2).Apply drugs to the back and observe the permeability change of cilia capillary,and auricle swelling degree in mice of each group.Results The curative criteria for Guiji groups was 3.8～4.1,the inhibition rate of auricular swelling was 75％.GuiJi cream could inhibit xylene-induced ear edema (P＜0.05) ; the Inhibition rate of vascular permeability was 45.08％,The ear swelling induced by xylene and increased capillary permeability induced by acetic acid in mice were inhibited significantly (P＜0.05) by Guiji cream which had obvious effects on fissure as well as the permeability.Conclusion Guiji chapped skin cream played significant functions in treating fissure and acting anti-inflammatory.

Objective To investigate the role of signal transduction of toll-like receptors(TLRs)in immunological pathogenesis in children with acute idiopathic thrombocytopenic purpura(ITP).Methods Thirty children with actue ITP and 30 age-matched healthy children were studied.Real-time fluorescent quantitative PCR was used to evaluate the levels of TLR 1-10 and signal transducing molecules,and cytokines associated with TLRs,such as IL-1?,granulocyte-macrophage colony-stimulating factor(GM-CSF),macrophage colony-stimulating factor(M-CSF)and IFN-?/? mRNA.Expressions of co-stimulatory molecules such as CD40,CD80 and CD86 in mo-nocyte/macrophage(MC)was analyzed by flow cytometry.Results Compared with healthy control group,the mRNA levels of TLR3,7,8 and 9 in ITP group were significantly up-regulated(Pa0.05).Transcription le-vels of MyD88-dependent and-independent pathway molecules such as MD-2,MyD88,IRAK-4,TRAF6,TAK1,TRIF,TRAM,TBK-1 and IFN-? were significantly up-regulated in acute ITP(Pa0.05).Conclusion Aberrant activation of toll-like receptors signaling may be one of the initiating factors of immune dysfunction in children with acute ITP.

Arachnoid ; pathology ; surgery ; Bone Neoplasms ; surgery ; Humans ; Male ; Middle Aged ; Neurilemmoma ; surgery

Adolescent ; Adult ; Aspergillosis ; complications ; microbiology ; Aspergillus ; isolation & purification ; Candida albicans ; isolation & purification ; Candidiasis ; complications ; microbiology ; Female ; Follow-Up Studies ; Granulomatosis with Polyangiitis ; complications ; microbiology ; pathology ; Humans ; Male ; Middle Aged ; Mucor ; isolation & purification ; Mucormycosis ; complications ; microbiology ; Nocardia ; isolation & purification ; Nocardia Infections ; complications ; microbiology ; Retrospective Studies ; Young Adult