Objective To study COX-2 expression in bladder transitional cell carcinoma in order to explore the relationship between the immunohistochemical staining and biological behavior of bladder cancer. Methods The expression of COX-2,Ki-67,and VEGF was examined in 50 cases of bladder transitional cell carcinoma and 10 normal bladder mucosa by means of immunohistochemical technique.Results The expression of COX-2 in BTCCs was higher than that in normal bladder mucosa.The positive rate of COX-2 was 80% in BTCCs while it was not detected in normal bladder mucosa.COX-2 was highly positively related to the grade,stage and prognosis of tumors.The expression of COX-2 in poorly differentiated,invasive or re- curring BTCCs was higher than that in well differentiated superficial or nonrecurring ones.But there was not significant difference between the solitary tumour and multiple ones.There were positive correlation both be- tween COX-2 and Ki-67 and between COX-2 and VEGF.Conclusion The high expression of COX-2 may benefit increase in bladder cancer cell proliferative activity.VEGF expression in COX-2 positive tumor was significantly higher than that in COX-2 negative tumor.

[Objective]To investigate the efficacy of tissue-engineerring artificial bone in treating rabbit bone defect.[Method]Rabbit bone marrow stromal cell were separated for cell culture,and subculture was done to increase the number of bone marrow stromal cell.A combined grafting material was made of bone marrow stromal cell and bioglass.The mixture,ilium and bioglass were used respectively to repair segment defects created in the radius of rabbits.The repair capability was assessed by figuration,radiography,histochemistry stains,scanning electron microscopy and biomechanical analysis at 12 weeks after operation.[Result]The amount of new bone formation with tissue-engineering artificial bone was more than that of other 3 groups.At 12 weeks the defect was bridged with the appearance of marrow cavities,but the bony healing was not found in the blank controls,which were repaired by fibrous tissue.After 12 weeks,the new bone formation in the group of the combined bone marrow stromal cells and bioglass showed the best performance.There was significant difference between groups.[Conclusion]Bone marrow stromal cell is a kind of good bony stem cell.The mechanism of osteogenesis may attribute to both intramembranous and endochondral ossification.The tissue engineering structuring bone marrow stromal cell is a promising method to repair bone defects.

AIM: To investigate the efficacy of 23G minimally vitrectomy without irrigation in cataract phacoemulsification and trabeculectomy of malignant glaucoma, and to analyze such compound operative procedures for phakic malignant glaucoma.
●METHODS:A total of 21 phakic malignant glaucoma patients (21 eyes) underwent anterior vitrectomy without irrigation by using 23G vitrectomy. During surgical course phacoemulsification with anterior and posterior continuous circular capsulorhexis, trabeculectomy combined with iridectomy would be completed. lntraocular pressure, anterior chamber depth changes and postoperative complications were observed after the operation.
●RESULTS:ln the three-month follow-up, intraocular pressures were reduced from ( 57. 18 ± 6. 18 ) mmHg to (16. 15 ± 2. 43 ) mmHg, there was statistical difference compared with pre - operation ( P < 0. 001 ). The preoperative anterior chamber depth (ACD) was (0. 88± 0. 25) mm, the postoperative ACD was (2. 44±0. 37) mm 3mo later, there were significant difference (P<0. 001). The best corrected visual acuity improved significantly, no serious postoperative complication appeared.
● CONCLUSION: The compound surgical method of anterior vitrectomy combined with phacoemulsifier and trabeculectomy can effectively treat phakic malignant glaucoma. Early diagnosis and early compound surgery may effectively reduce the intraocular pressure of malignant glaucoma.

Adult ; DNA, Viral ; Female ; Hepatitis B virus ; Humans ; Lamivudine ; therapeutic use ; Liver Neoplasms ; drug therapy ; genetics ; virology ; Male ; Middle Aged ; Point Mutation ; Retrospective Studies

Bacterial Proteins ; metabolism ; Carbon-Sulfur Lyases ; metabolism ; Mouth ; microbiology ; Quorum Sensing ; Signal Transduction

Berberine Alkaloids ; therapeutic use ; Humans ; Phytotherapy ; Tachycardia ; drug therapy

Humans ; Occupational Health ; Protective Devices ; utilization ; Welding

Objective To identify related anatomical parameters and the strongest risk predictor leading to patients with recurrent patellar instability.Methods 52 young patients with recurrent patellar instability that failed of conservative treatment were retrospec-tively evaluated by MRI.Measurements included parameters of patellar tracking,which was characterized by bisect offset index (BSO),trochlear congruence angle (CA)and patellar lateral tilt (PTA),and anatomical parameters of patellofemoral joint,which were characterized by the sulcus angle (SA),lateral trochlear inclination (LTI),trochlear facet asymmetry,trochlear depth,Insall-Salvati Ratio (ISR),Caton-Deschamps index (C-D)and tibial tuberosity-trochlear groove (TT-TG)distance.The collection data were analyzed including the Pearson's correlation and multiple stepwise regression analysis.Results The patellofemoral joint ma-lalignment was significantly correlated with LTI,TT-TG distance and ISR,but not or partly with the trochlear depth,SA,trochlear facet asymmetry and C-D.Linear regression models including LTI,TT-TG distance and ISR explained 0.58,0.47 and 0.43 of the va-riance in BSO,CA and PTA,respectively (P<0.001).And the standardized beta coefficient was largest for LTI,then were TT-TG distance and ISR.Conclusion At full extension of the knee,anatomical related factors measured on MRI explain some degree of re-current lateral patella shift and tilt,the strongest predictor among anatomical related factors that could lead to maltracking is LTI, and then are TT-TG distance and ISR.

ObjectiveTo investigate the effect of Du meridian electroacupuncture of the scalp on deglutition disorder after stroke.Methods67 patients with deglutition disorder after stroke were randomly divided into the experimental group (n=34) and control group (n=33). The patients of the experimental group were received Du meridian electroacupuncture of the scalp as main therapy, combined with routine medicine treatments; the patients of the control group were only received routine medicine treatments. Before and after treatments, deglutition function of all patients was assessed by drinking water test and clinical effect was also assessed.ResultsAfter treatment, the deglutition function of the patients in the experiment group was markedly improved and superior to those in the control group ( P<0.01).ConclusionDu meridian electroacupuncture can markedly improve the clinical effect of routine medicine treatments on deglutition disorder after stroke.

Objective To observe the therapeutic efficacy of Ai Luo Kang acupoint application in treating simple obesity. Methods Eighty simple obesity patients were randomized into a treatment group and a control group, 40 in each group. The treatment group was intervened by Ai Luo Kang acupoint application, while the control group was taken as a blank control. Thirty days later, the two groups were compared in the change of body weight and waist circumference. Results After intervention, the body weight and waist circumference were significantly changed in the treatment group (P＜0.01). There were significant differences between the two groups in comparing the body weight and waist circumference after intervention (P＜0.05). Conclusion According to the relevant criteria of the Evaluation Procedures and Methods of the Function of Health Care Products (DB22-T397-2006), Ai Luo Kang acupoint application is effective in losing weight in simple obesity.