1.Study on the effect of the low-frequency intense sound on animal behavior response.
Chang-chao GONG ; Xin-wu ZHENG
Chinese Journal of Applied Physiology 2008;24(4):492-503
Acoustic Stimulation
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Animals
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Auditory Perception
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physiology
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Behavior, Animal
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physiology
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Noise
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adverse effects
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Rabbits
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Sound
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Swine
2.Performance of cardiopulmonary resuscitation during prolonged basic life support in military medical university students: A manikin study
Juan WANG ; Chao-Nan ZHUO ; Lei ZHANG ; Yu-Shun GONG ; Chang-Lin YIN ; Yong-Qin LI
World Journal of Emergency Medicine 2015;6(3):179-185
BACKGROUND: The quality of chest compressions can be significantly improved after training of rescuers according to the latest national guidelines of China. However, rescuers may be unable to maintain adequate compression or ventilation throughout a response of average emergency medical services because of increased rescuer fatigue. In the present study, we evaluated the performance of cardiopulmonary resuscitation (CPR) in training of military medical university students during a prolonged basic life support (BLS). METHODS: A 3-hour BLS training was given to 120 military medical university students. Six months after the training, 115 students performed single rescuer BLS on a manikin for 8 minutes. The qualities of chest compressions as well as ventilations were assessed. RESULTS: The average compression depth and rate were 53.7±5.3 mm and 135.1±15.7 compressions per minute respectively. The proportion of chest compressions with appropriate depth was 71.7%±28.4%. The average ventilation volume was 847.2±260.4 mL and the proportion of students with adequate ventilation was 63.5%. Compared with male students, significantly lower compression depth (46.7±4.8 vs. 54.6±4.8 mm,P<0.001) and adequate compression rate (35.5%±26.5% vs. 76.1%±25.1%,P<0.001) were observed in female students. CONCLUSIONS: CPR was found to be related to gender, body weight, and body mass index of students in this study. The quality of chest compressions was well maintained in male students during 8 minutes of conventional CPR but declined rapidly in female students after 2 minutes according to the latest national guidelines. Physical fitness and rescuer fatigue did not affect the quality of ventilation.
3.Effects of tank operation on renal function of crews.
Qiang MA ; Hong-Fei WANG ; Chang-Jiang XING ; Hua-Chao MA ; Mei-Liang GONG ; Lei SUN ; Hong-Ling LIANG
Chinese Journal of Applied Physiology 2014;30(5):475-477
OBJECTIVETo explore the effects of harmful factors in tank cabins on renal function of tank crews.
METHODSOne hundred and fifty two tank crews as the observation group and 37 soldiers without tank environment exposure as control group were selected in the study. α1-microglobulin(α1-MG), β2-microglobulin(β2-MG), IgG, N-acetyl-β-glucosidase (NAG) and urinary albumin excretion rate (UAER) in morning and 24 h urine were measured.
RESULTSCompared to the control group, the levels of α1-MG, β2-MG, NAG, UAER in observation group were increased significantly (P < 0.05). β2-MG, NAG, UAER of Soldiers with more than 50 motorized hours in observation group were significantly higher than those of control group (P < 0.05). β2-MG, NAG and UAER of soldiers divorced from tank occupation more than 3 years decreased to the normal levels. β2-MG of soldiers divorced from tank occupation more than 10 years was significantly higher than that of 6-10 years group.
CONCLUSIONTank occupational exposure influences the renal function of tank crews but not to a degree of clinical kidney disease. The renal function of crews divorced from tank occupation may recover but dysfunction of renal tubular reabsorption still exists.
Acetylglucosaminidase ; metabolism ; Albuminuria ; Alpha-Globulins ; metabolism ; Environmental Exposure ; adverse effects ; Humans ; Kidney ; physiology ; Kidney Function Tests ; Military Personnel ; beta 2-Microglobulin ; metabolism
4.Adjuvant hormonal therapy immediately after radical surgery for high-risk organ-confined or locally advanced prostate cancer.
Zhao-Long GUAN ; Liang HUANG ; Rui-Zhe ZHAO ; Gong CHENG ; Chao QIN ; Peng-Fei SHAO ; Jie LI ; Li-Xin HUA ; Chang-Jun YIN
National Journal of Andrology 2014;20(12):1093-1097
OBJECTIVETo evaluate the effect of adjuvant hormonal therapy (AHT) immediately after radical surgery for high- risk organ-confined or locally advanced prostate cancer using the PSA-related biochemical relapse rate within 2 years after surgery.
METHODSWe retrospectively analyzed 62 cases of high-risk organ-confined or locally advanced prostate cancer. The patients were treated by laparoscopic radical prostatectomy or radical retropubic prostatectomy after MRI and ECT systemic bone imaging examination, which revealed no regional lymph node or bone metastasis. Thirty-two of the patients (group A) received AHT orally or subcutaneously from 2 weeks to 1 months after operation, and another 30 (group B) were left untreated. We followed up the patients for 2 years, measuring the serum PSA level every 3 months, performing ECT every 6 months, and recording the adverse reactions, medication dura- tion, and the patients'quality of life.
RESULTSAll the operations were successfully accomplished. The rate of 2-year biochemical relapse-free survival was 78.13% (25/32) in group A and 53.33% (16/30) in group B.
CONCLUSIONAHT immediately after radical surgery can improve the rate of biochemical relapse-free survival of the patients with high-risk organ-confined or locally advanced prostate cancer and check the progression and metastasis of the disease.
Aged ; Antineoplastic Agents, Hormonal ; therapeutic use ; Chemotherapy, Adjuvant ; Disease Progression ; Disease-Free Survival ; Humans ; Laparoscopy ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; blood ; Neoplasm Staging ; Prostate-Specific Antigen ; blood ; Prostatectomy ; methods ; Prostatic Neoplasms ; blood ; drug therapy ; pathology ; surgery ; Quality of Life ; Retrospective Studies
5.Testis-sparing surgery for benign testicular tumor.
Huang SU ; Bian-jiang LIU ; Ning-hong SONG ; Peng-chao LI ; Gong CHENG ; Jie YANG ; Zeng-jun WANG ; Li-xin HUA ; Chang-jun YIN
National Journal of Andrology 2014;20(11):1020-1024
OBJECTIVETo investigate the safety and feasibility of testis-sparing surgery (TSS) in the treatment of testicular tumor.
METHODSWe retrospectively analyzed the clinical data of 8 cases of benign testicular tumor treated by TSS in our hospital from October 2005 to March 2012.
RESULTSThe 8 patients, aged 18-67 (mean 45) years, were preoperatively diagnosed with benign testicular tumor and all underwent partial testis resection. Rapid intraoperative pathology showed the incisal margins to be negative. Postoperative pathological examination confirmed Sertoli cell tumor in 3 cases, adenomatoid tumor in another 3, and mature teratoma in the other 2. The patients were followed up for 6 months to 7 years (mean 4 years), which revealed no relapse and metastasis, nor significant differences from the baseline in the testosterone level, IIEF score, and routine semen parameters.
CONCLUSIONTestis-sparing surgery is one of the effective options for the management of benign testicular tumor, which can maximally preserve the testis tissue and protect the patient's sexual function.
Adolescent ; Adult ; Aged ; Humans ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Organ Sparing Treatments ; methods ; Retrospective Studies ; Sertoli Cell Tumor ; pathology ; surgery ; Teratoma ; pathology ; surgery ; Testicular Neoplasms ; pathology ; surgery ; Testis
6.Study on Ambi-extracting and Inclusion Process of Volatile Oil from Chuanxiong Rhizoma and Angelicae Sinensis Radix
Mei-Ling WANG ; Jian NI ; Lin GUO ; Gong-Sen CHEN ; Ying-Chao YU ; Long-Tai YOU ; Hai-Ying ZHANG ; Xing-Bin YIN ; Chang-Hai QU
Chinese Journal of Information on Traditional Chinese Medicine 2018;25(11):68-72
Objective To optimize ambi-extracting and inclusion process of volatile oil from Chuanxiong Rhizoma and Angelicae Sinensis Radix. Methods With yield ratio of volatile oil and ferulic acid content in water extract as evaluation indexes, single factor experiments were used to study the extraction process. With the inclusion rate of volatile oil and yield of inclusion as evaluation indexes, saturated aqueous solution was used to L9(34) orthogonal experiments to reach optimum inclusion process. Results The optimum extraction process of Chuanxiong Rhizoma and Angelicae Sinensis Radix was extracted for 8 hours with 8 folds the amount of water, and without soaking. The validation experiments of extraction of volatile oil and ferulic acid content in water extract were 1.23 mL and 0.387 9 mg/g. The optimum conditions of inclusion process were as follows: volatile oil (mL): β-CD (g) was 1:8;inclusion temperature was 40 ℃; inclusion time was 3 hours. The validation experiments of inclusion rate of volatile oil and yield of inclusion were 74.89% and 72.81%. Conclusion Optimum ambi-extracting and inclusion process of volatile oil from Chuanxiong Rhizoma and Angelicae Sinensis Radix are feasible and stable, witch can provide certain supporting data for preparation production.
7.Study on the RNA of severe acute respiratory syndrome (SARS) associated coronavirus in the blood and excretion of convalescent patients with SARS.
Zhao-rui CHANG ; Ren-quan YANG ; Yan-bin WANG ; Li-li REN ; Min WANG ; Yao-wu YANG ; Li GUO ; Yan-gong CHAO ; Cheng-yi QU ; Jian-wei WANG ; Tao HONG
Chinese Journal of Epidemiology 2005;26(3):201-203
OBJECTIVETo examine the RNA of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) in the blood and excretion of convalescent patient with SARS for prevention and treatment of the disease.
METHODSA total of 276 samples, including plasma, urine, feces and sputum, obtained from 23 convalescent patients with SARS were studied at 3 time-points at least 21 days after the onset of symptoms. RNA was extracted and nested reverse transcription-polymerase chain reaction (RT-PCR) was carried out using SARS-CoV specific primers.
RESULTSAmong the 276 samples, SARS-CoV RNA was detected in 6 cases (38.8%) by nested RT-PCR. The positive rates of SARS-CoV RNA was 5.8% in feces and 2.9% in sputum samples but SARS-CoV RNA was not detectable in plasma and urine of all the cases.
CONCLUSIONThe existence of SARS-CoV RNA in the excretion of some convalescent patients with SARS showed that the excretion from these patients should be carefully treated whilthe re-transmission of SARS by which, should be further studied.
Adolescent ; Adult ; Aged ; Convalescence ; Feces ; virology ; Female ; Humans ; Male ; Middle Aged ; RNA, Viral ; analysis ; blood ; Reverse Transcriptase Polymerase Chain Reaction ; SARS Virus ; isolation & purification ; Severe Acute Respiratory Syndrome ; virology ; Sputum ; virology
8.miR-15b suppresses glioma stem cell migration and invasion by targeting ABCG2 signaling pathway
feng Yi LIU ; chao Bao ZHANG ; ming Chang WEN ; ling Gong WEN ; ping Guo ZHOU ; wei Jing ZHANG ; fa Hai HE ; Ning WANG ; Wei LI
Chinese Journal of Tissue Engineering Research 2017;21(33):5305-5312
BACKGROUND: miR-15b plays an important role in the initiation and development of tumors, based on which, we speculate that miR-15b may be involved in the migration and invasion of glioma stem cells (GSCs). However, there is no relevant report and the mechanism of action is also unclear. OBJECTIVE: To identify the effects of miR-15b on the migration and invasion of GSCs and the mechanisms involved in this process. METHODS: Quantitative PCR was performed to evaluate the expression of miR-15b in the gliomas tissues, normal brain tissues, GSCs and non-GSCs. After knockdown of ATP-binding cassette superfamily G member 2 (ABCG2) by ABCG2 specific siRNA, Transwell assay was performed to determine the effect of ABCG2 on GSCs migration and invasion. Additionally, the GSCs were transfected with miR-15b mimics or inhibitor to up-regulate or down-regulate the expression of miR-15b. At 48 hours after transfection, Transwell assay was used to detect the effect of miR-15b on GSCs migration and invasion; ELISA and gelatin zymography assays were performed to determine the matrix metalloproteinase-2/-9 (MMP-2/-9) expression and activity after treatment with miR-15b. CD133-positive or non-CD133-positive cells were directly injected subcutaneously into nude mice. Tumor formation was observed within 30 days after injection. RESULTS AND CONCLUSION: miR-15b was significantly down-regulated in gliomas tissues compared with normal brain tissues, which was negatively correlated with the stage of gliomas. In addition, miR-15b was significantly down-regulated in GSCs compared with non-GSCs. Up-regulation of miR-15b significantly reduced the migration and invasion ability of GSCs (P < 0.01), and down-regulation of miR-15b significantly enhanced the cell migration and invasion of GSCs (P < 0.01). By target prediction analysis, we obtained that ABCG2 was a potential target gene of miR-15b. Luciferase assay confirmed that miR-15b targeted ABCG2 directly, and migration and invasion of GSCs were dramatically reduced by ABCG2 siRNA (P < 0.01). ELISA results showed that up-regulation miR-15b significantly inhibited MMP-2/-9 expression. ELISA and gelatin zymography assay results showed that ABCG2 siRNA did not affect MMP-2/-9 expression, but significantly inhibited the activity of MMP-2/-9. In the in vivo tumor model, GSCs were more tumorigenic as compared with non-GSCs from the same tumor in vivo. To conclude, miR-15b regulates the migration and invasion of GSCs by targeting the ABCG2 signaling pathway, and up-regulation of miR-15b can suppress the migration and invasion of GSCs.
9.Preparation and characterization of monoclonal antibodies against SARS-associated coronavirus nucleocapsid protein.
Yan-bin WANG ; Zhao-rui CHANG ; Jian-wei WANG ; Rong JI ; Chun-hui HAN ; Li ZHAO ; Li-li REN ; Yan-gong CHAO ; Jian-guo QU ; Cheng-yi QU ; Tao HUNG
Chinese Journal of Experimental and Clinical Virology 2004;18(4):316-320
OBJECTIVETo obtain monoclonal antibodies (McAbs) against severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) nucleocapsid (N) protein to develop diagnostic test for SARS and study the pathogenesis of the disease.
METHODSBALB/c mice were immunized with purified N protein of SARS-CoV. Hybridoma cell lines secreting monoclonal antibodies against SARS-associated coronavirus nucleocapsid were established after cell fusion with mouse splenic cells and SP2/0 cells. The specificity of the McAbs obtained was examined by Western blot and indirect fluorescence assay. Epitopes reacted with the McAbs were preliminarily located through Western blot by expressing truncated N proteins.
RESULTSAfter cell fusion and three rounds of cell cloning, six hybridoma cell lines secreting monoclonal antibodies specifically against SARS-CoV nucleocapsid were obtained. Western blot and indirect fluorescence assay showed that the McAbs reacted specifically with nucleocapsid protein and SARS-CoV. Among the six McAbs, three recognize the epitopes located in the N-terminus of the protein, whereas the others reacted with those located in the C-terminus.
CONCLUSIONThe anti-SARS-CoV nucleocapsid McAbs were developed and these McAbs may be useful in the development of diagnosis assays and basic research of SARS.
Animals ; Antibodies, Monoclonal ; biosynthesis ; immunology ; Antibodies, Viral ; biosynthesis ; immunology ; Antibody Specificity ; Female ; Hybridomas ; secretion ; Mice ; Mice, Inbred BALB C ; Nucleocapsid Proteins ; immunology ; isolation & purification ; SARS Virus ; chemistry ; immunology
10.Preparation and quality evaluation of levocarnitine thermosensitive in situ gel
Ping-qing HUANG ; Li-li GAO ; Ying-chao YU ; Yi-bo WANG ; Hui-min WU ; Gong-sen CHEN ; Jian NI ; Chang-hai QU
Acta Pharmaceutica Sinica 2019;54(6):1115-1122
In this paper, a new type of preparation for treatment of initial dry eye disease, thermosensitive