Objective To evaluate the treatment effects of double parabolic lingual springs appliance and straight wire appliance therapy in skeletal class Ⅲ malocclusion. Methods 12 cases with skeletal class Ⅲ malocclusion were treated by double parabolic lingual springs appliance and straight wire appliance, cephalometric tracing measurement were taken in analysis,compared between pretreatment and posttreatment in each case. Results The comparison of before and after orthodontic treatment showed angle SNA increased by 2.80°, angle SNB decreased 1.39°ANB decreased by 4.88°. Conclusion The above metioned technique could be a reliable way to treat mild and moderate skeletal class Ⅲ malocclusion.

Although choledochoscope has been described as an ideal approach for cholelithiasis, larger,impacted or residual biliary duct stones could hardly be taken out. Choledochoscopic laser lithotripsy was used in 1978. At present, however, it has not wildly been applied in the biliary surgery. In this article, we reviewed the domestic and foreign literatures to state the application and prospect of choledochoscope combined with laser lithotripsy in cholelithiasis.

China ; epidemiology ; Humans ; Precancerous Conditions ; diagnosis ; epidemiology ; pathology ; Stomach Neoplasms ; diagnosis ; epidemiology ; pathology ; Topography, Medical

Objective To investigate the relationship between the osteopontin ( OPN ) expression of colorectal cancer tissues with the clinical characteristics and prognosis. Methods The expressions of OPN in colorectal cancer tissues and their corresponding para-cancerous tissues from 112 cases of colorectal cancer patients were detccted by immunohistochemistry . The relationship between the expressions of OPN and the clinicopathological features in pait-ients with colorectal cancer were analyzed. Results The OPN expression of colorectal cancer tissues was signifi-cantly higher than their corresponding adjacent normal tissues, the difference was statistically significant ( P <0. 05). Its expression was significantly associated with tumor size, histological differentiation, TNM stage, tumor status. Disease-free survival, and overall survival time of OPN protein positive expression group were lower than that of the negative group, respectively, the differences were statistically significant(P<0. 05). The expression of OPN in colorectal cancer tissues was an independent prognostic factor. Conclusion The expression of OPN is sig-nificantly increased in colorectal cancer tissues, and the positive expression of OPN is associated with the prognostic of patients with colorectal cancer.

Humans ; Larynx ; surgery ; Neurilemmoma ; surgery

Objective: To analyze the efficiency of chronic disease prevention and control of primary health service institutions in Shandong, and provide policy references. Methods:Using CRS and VRS models from DEA to analyze the representative input and output indicators in primary health Service institutions. Results: The overall efficiency of chronic disease prevention and control in primary health service institutions is low, and the actual output was insufficient compared with the target output. Conclusion: Strengthening chronic disease control measures in high-risk group and establishing management mechanism leaded by the goal of chronic prevention and control in order to achieve optimal allocation of resources.

OBJECTIVE To explore the effect of chloride channels on the neuronal injury following cerebral ischemia. METHODS Tweleve day in vitro (12dIV) neurons in rats were randomly divided into normal control, 3-morpholinosydnonimine (SIN-1, 1.0 mmol·L~(-1) for 18 h) group, SIN-1+4-acetamido-4'-isothiocyanatostilbene-2,2'-disulphonic acid(SITS, 0.5 mmol·L~(-1)) group and SIN-1+4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS, 0.1 mmol·L~(-1)) group. Drugs were added with SIN-1 simultaneously and coincubated for 18 h. The neuronal apoptosis and morphological changes were detected with Hoechst 33258. Chloride channels(ClC)-2/ClC-3 were analyzed with immunofluorescence, the chloride channel currents were recorded with whole cell patch-clamp technique. RESULTS Hoechst 33258 staining showed that the apoptotic percentages were (18.61±0.59) %, (50.43±0.56)%, (23.37±0.52)% and (23.37±0.84)% in normal control group, SIN-1 group, SIN-1+SITS or SIN-1+DIDS groups, respectively. ClC-2/ClC-3 were positively expressed in normal neurons. The currents in neurons exposed to SIN-1 were increased about 55%-56%, SITS and DIDS, two kinds of chloride channel blockers could inhibited the currents about 50%-60% and 30%-40%, respectively. CONCLUSION Voltage-dependent chloride channel maybe participate in the neuronal apoptosis induced by NO, and the activities of chloride channels are perhaps involved in the cerebral ischemic injury.

BACKGROUND: Studies showed cellular adhesion molecule and neurotrophic factor secreted from olfactory ensheathing cells (OECs) could protect the spinal neurons and promote the regeneration of spinal axon. OBJECTIVE: To compare the competence to repair spinal cord injury between olfactory mucosa OECs and olfactory bulb OECs. DESIGN, TIME AND SETTING: Randomized control animal experiment was performed in the central laboratory of Xidian Group Hospital between June 2007 and June 2008. MATERIALS: Twelve male SD rats were randomized selected and divided into experiment group (n=6, 23 months old) and control group (n=6, 3 months old). They were used for in vitro culture and purification of OECs; other 30 SD rats were randomized into three groups of 10 rats each: neonatal rat olfactory bulb OECs transplantation group, normal olfactory mucosa OECs transplantation group and blank control group.METHODS: Spinal cord injury models were produced in 30 rats, which were transplanted with the neonatal rat or SD rat OECs cultured in vitro. No transplant was given in the control group. MAIN OUTCOME MEASURES: At 4 and 8 weeks postoperation, the Basso, Beattie, Bresnahan (BBB) score for nerve function, the evoked potential of legs and the histopathological diversify of injured spinal cord. RESULTS: Seven rats died dudng the experiment process, and the death rate was similar between groups. At 4 and 8 weeks postoperation, there was no significant difference in the BBB scores between neonatal rat olfactory bulb OECs transplantation group and normal olfactory mucosa OECs transplantation group (P > 0.05), which were both significantly higher than blank control group (P < 0.001); the BBB scores in two transplantation groups were higher at 8 weeks than at 4 weeks (P < 0.01 ). At 4 weeks postoperaUon, no animal was shown to elicit motion evoked potential, but it was present in two transplantation groups at 8 weeks, with no significant difference between two groups (P > 0.05). The blank control group had still no motion evoked potential (P < 0.001 ). At 8 weeks postoperation, more cell infiltrations were found in the injured spinal cord of two transplantation groups, while few in the control group.CONCLUSION: Both OECs dissociated from olfactory bulb and olfactory mucosa have the same ability to repair the injured spinal cord, and their effect is similar.

Objective To observe the effect of Yiqi Huaxian Decoction (YHD) on transforming growth factor ?1(TGF-?1 )mRNA expression in rats alveolar macrophages (AM) and pulmonary interstitial macrophages (IM).Methods Ninety-six Wistar rats were randomized into sham-operation group,model group,YHD (4 g?kg-1?d-1) group,and hydrocortisone (20 mg?kg-1?d-1) group.Bleomycin A5 was injected into the rats' trachea to induce pulmonary fibrosis.AM and IM were isolated for the culturing after modeling for 7 and 28 days.Molecular in-situ hybridization (ISH) was used for the detection of TGF-?1 expression in AM and IM.Results Percentage of cells in AM and IM with TGF-?1 positive expression and the score of optic density were increased in the model group (P

Objective To study the influence of edaravone on malondialdehyda(MDA)and expression of Hsp-70 and Bcl-2 in the perihematoma region in rats.Methods A total of 120 health male rats were randomly divided into false-operation group(n=20)、intracerebral hemorrhage(ICH)-therapy group(n=50)and ICH control group.Each group was further divided into 5 subgroups respectively according to 6 h、24 h、48 h、72 h and 5d after model creation.Cerebral hemorrhage model was duplicated with the method created by Fredrik.Brain water content and MDA were measured.Expression of Hsp-70 and Bcl-2 were assayed in each group with immunohistochemical method.Results Brain water content and MDA were lower in ICH-therapy group than those in control group(P