2.Risk factors for lower extremity lymphedema following treatment of gynecologic cancers:a meta-analysis
Xin CHANG ; Jiaofeng SHEN ; Qiliang PENG ; Zhixiang ZHUANG ; Ye TIAN
Chinese Journal of Radiation Oncology 2017;26(9):1038-1044
Objective To investigate the multiple risk factors for lower extremity lymphedema in patients following treatment of common gynecologic cancers by meta-analysis for systematic analysis and comprehensive quantitative study.Methods Clinical trials published up until August 2016 were retrieved from PubMed, Embase, and the Cochrane Library.The quality of the included studies was assessed by the Newcastle-Ottawa Scale, and data analysis was performed using Stata 14.0 and RevMan 5.3.The strength of the associations between risk factors and gynecologic cancer-related lower extremity lymphedema was described as odds ratio (OR) and 95% confidence intervals (CI).Results Eighteen studies were included in the meta-analysis, and 8 relevant factors were identified.The risk factors for lower extremity lymphedema after treatment of gynecologic cancer mainly included radiotherapy (OR=2.45, 95%CI:2.05-2.95, P=0.000), FIGO stage (OR=2.29, 95%CI:1.66-3.14, P=0.000), and pelvic lymph node dissection (OR=2.00, 95%CI:1.02-3.91, P=0.040).Conclusions Radiotherapy, FIGO stage, and pelvic lymph node dissection are the main risk factors for lower extremity lymphedema after treatment of gynecologic cancers.
3.The analysis of the complete genome sequence of swine hepatitis E virus isolate swGX32
Yanli JI ; Lingjun LI ; Xianfei WEI ; Ling WANG ; Yibin CHANG ; Ronglan TANG ; Yonghong ZHU ; Hui ZHUANG
Chinese Journal of Microbiology and Immunology 2008;28(5):421-425
Objective To analyze the complete genome sequence of Guangxi HEV isolate swGX32 and to compare it with other HEV isolates. Methods The overlapping fragments of HEV isolate swGX32 were amplified with reverse-transcription nested polymerase chain reaction (RT-nPCR),and the 5′ and 3′ ends of viral genome were amplified with rapid amplification of cDNA ends (RACE). The PCR products were cloned and sequenced. The sequence and phylogenetic analysis of swGX32 was performed. Results The genome of swGX32 consisted of 7240 nt excluding the polyA tail, with 4 nt overlapping between ORF1 and ORF2. ORF3 is contained in the sequence of ORF2. The complete genome sequence of swGX32 shared identity of 73%-74%, 73%, 74%-75%,83%-94% with HEV genotype 1,2,3 and 4, respectively. Among all these HEV reference sequences, swGX32 showed the highest identity with the human isolate JKO-ChiSai98C (94%). Phylogenetic tree showed that swGX32 belonged to genotype 4 and clustered with JKO-ChiSai98C in the branch of HEV subtype 4a. Conclusion The swine HEV isolate swGX32 is closely related to human strain JKO-ChiSai98C genetically and phylogenetically, which further provides molecular biology evidence of hepatitis E as a zoonosis.
4.An in vitro study on influence of intravenous immunoglobulin on function of T cells in cord blood of preterm infants
Hong CHANG ; Qiuye ZHANG ; Chao ZHUANG ; Hongling SHENG ; Mengmeng WU ; Haiyan ZHU
Chinese Journal of Perinatal Medicine 2013;16(12):766-769
Objective To observe the influence of different doses of intravenous immunoglobulin (IVIG) on function of T cells in cord blood of premature infants and to explore the immunomodulating mechanism of IVIG.Methods Cord blood mononuclear cells (CBMC) were isolated by density gradient centrifugation method from 15 preterm infants born between June 1,2011 to December 31,2011 at 32 34 gestational weeks.Three groups were formed according to the concentrations of IVIG used for CMBC culturing in vitro (Group A,6 mg/ml; Group B,9 mg/ml; Group C,12 mg/ml).After 72 hours in sterile conditions,the levels of IFN 7,IL 4,IL-10 and TGF β1 in the supernatant were determined by enzyme-linked immunosorbent assay.The expression of CD4+ CD25+ Foxp3+ Treg cells was examined by fluorescent activated cell sorter.Differences among groups were compared by one way analysis of variance.For comparison between two groups,LSD test was used.Results (1) The level of IFN γ secreted by CBMC in Group C was 0.03 ± 0.02,which was much lower than that in Group A(0.18±0.08) and Group B(0.13±0.05) (F=5.72,both P<0.01).The level of IFN-γ in Group B and Group A did not show statistical difference (P>0.05).Compared with Group A(0.03±0.01),the level of IL4 was much lower in Group B (0.02±0.01) and Group C(0.01±0.01) (F=20.38,both P<0.01),while no significant difference was shown between Group B and Group C (P>0.05).(2) No significant difference was found in the expression of CD4+CD25+Foxp3+Treg cells among different groups (F 0.67,P>0.05).(3) The level of IL 10 in Group C was 3.02 ± 3.79,which was significantly lower than that in Group A (10.78±5.44) and Group B (6.90±4.64)(F=4.68,P<0.01 and 0.05).The level of IL-10 inGroup B was still lower than that in Group A (P<0.05).The levels of TGF-β1 in Group C(8.44± 13.71) and Group B(16.15 ±13.94) were significantly lower than that in Group A(30.23 ± 16.32) (F=5.22,P<0.01,P<0.05),but there was no significant difference between Group B and Group C (P>0.05).Conclusions IVIG might inhibit the function of Th cells in CBMC of preterm infants in a dose dependent manner.IVIG could inhibit the function of natural Treg cells by regulating the secretion of cytokines IL-10 and TGF-β1 in a dose dependent effect.
5.Analysis of Polyimide Resin byReversed Phase Ion Pair Suppression Chromatography
Guiyun XU ; Yimei SHANG ; Shuying ZHANG ; Xiaochun WANG ; Zhu ZHAO ; Shiyong YANG ; Liwen CHANG ; Zhuang LIU
Chinese Journal of Analytical Chemistry 2001;29(4):434-436
This paper describes the analysis of insitu polymerization of monomeric reactants (PMR) polyimide resin solution by reversed phase ion pair suppression chromatography. the mobile phase consists of acetonitrile and water. The methyl sulphonic acid was used as an ion-pair reagent in the mobile phase. The addition of sodium perchlorate was required to increase the ionic strength of the mobile phase. The information was obtained on monomeric isomer distribution of PMR polyimide resin solution and purity of prepared materials.
6.Perinatal management and outcomes of triplet and multiple pregnancy
Chun YANG ; Yong WANG ; Daofang WANG ; Qimei WANG ; Hong CHANG ; Mingfeng ZHUANG
Chinese Journal of General Practitioners 2008;7(6):381-383
Objective To analyze clinical management and outcomes of perinatal fetuses born in triplet and multiple pregnancy.Methods Data of clinical management and outcomes of 55 perinatal fetuses born during 1996 to 2005 by women with triplet and multiple pregnancy were analyzed retrospectively.Results Antenatal check-up was performed regularly for 1/6 of those pregnant women during 1996 to 2000,as compared to that of 8/11 during 2001 to 2005(P<0.05).No significant difference in incidence of varied obstetric complications was found between those during 1996 to 2000 and during 2001 to 2005.Average gestational week at birth was(32.7±2.8)and(35.1±1.9)weeks(P<0.05),and averagebirth weight was(1561±471)grams and(1987±453)grams(P<0.01),respectively for them during 1996 to 2000 and during 2001 to 2005.Caesarean section was performed more for the pregnant women in the earlier first five years than in the later five years,but not reaching statistical significance.Incidence of neonatal complications significantly decreased in the later five years than that in the earlier five yeats,including hyaline membrane disease,neonatal asphyxia,infectious diseases,intracranial hemorrhage and pulmonary hemorrhage(P<0.05),but difference in incidence of apnea and low body temperature between the earlier and later five years did not reach statistical significance.Perinantal mortality was 8 in 35 births in the later five years,as compared to that of 12 in 20 births in earlier five years(P<0.01).Conclusions Outcomes of perinatal fetuses in triplet and multiple pregnancy can be improved by combined action of obstetricians and pediatricians,including regular antenatal examination,active prevention and treatment for pregnant complications and preterm infant diseases,earlier admission waiting for delivery to prolong gestational weeks and increase birth weight,and applying antenatal dexamethasone therapy.
7.Technical improvement in dealing with chronic atrophic gastritis samples.
Hong-xi MA ; Hai-feng WANG ; Zhuang TIAN ; Chang-yan XU
Chinese Journal of Pathology 2012;41(9):637-638
Adult
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Aged
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Aged, 80 and over
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Female
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Gastritis, Atrophic
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diagnosis
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pathology
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Gastroscopy
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methods
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standards
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Humans
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Male
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Middle Aged
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Specimen Handling
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methods
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Tissue Embedding
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methods
8.Expression, Purification of Human Endostatin in Pichia pastoris and the Detection of Its Anti-angiogenic Activity
Xue-Jing YAO ; Zhuang-Lin LI ; Guo-Dong CHANG ; Gui-Yong YUAN ; Xue- YU ;
China Biotechnology 2006;0(04):-
Endostatin is a potent, endogenous inhibitor of angiogenesis, which corresponds to the C-terminal fragment of collagen ⅩⅧ. The human endostatin gene was amplified from a human fetal liver cDNA library by means of PCR and was cloned into pPIC9K vector. Soluble endostatin was superexpressed in Pichia pastoris (50.5mg/L) . The recombinant protein was purified by cation exchange chromatography with the final yield of more than 95%. Western blotting analysis showed positive immunoreactivity to the expressed product. Recombinant endostatin was able to suppress the angiogenesis on the CAMs. Also, the endostatin inhibited specifically of the migration of HMECs stimulated by bFGF, with EC50 being about 0.4 ?g/ml.
9.Correlative study between MRI signal intensity of the lumbar intervertebral disc and collagen content in the nucleus pulposus
Wen-Chang YU ; Jian-Yong YANG ; Wen-Quan ZHUANG ; Yun-Bin CHEN ; Shi-Fang ZHENG ;
Chinese Journal of Radiology 1999;0(10):-
Objective To study correlation between MRI signal intensity of the lumbar intervertebral disc and collagen content in the nucleus pulposus.Methods Thirty-one cases with lumbar intervertebral disc herniation(male 21,female 10)received percutaneous lumbar disketetomy.Thirty-one specimens of nucleous pulposus were obtained from percutaneous lumbar disketctomy procedure and collagen content in them was measured with reformed hydroxyproline measurement method.The signal intensity(SI) of lumbar intervertebral disc and cerebrospinal fluid was measured in T_2 WI sagittal image and then ratio of disc SI to cerebrospinal fluid SI was calculated.The Pearson analysis was used to analyze the correlation between the collagen content and SI ratio and disc SI on T_2 WI.Results Collagen content and SI ratio were (231.0?63.5)mg/g and 0.19?0.07,respectively.There was negative correlation between them (r=-0.61,P
10.The choice of anterior approach for acetabular fractures: a systematic review
Binfei ZHANG ; Shuang HAN ; Wei FAN ; Ning CHANG ; Hu WANG ; Xing WEI ; Pengfei WANG ; Yan ZHUANG
Chinese Journal of Orthopaedics 2017;37(13):801-809
Objective To evaluate the efficacy and safety in the treatment of acetabular fractures via anterior approaches,including ilioinguinal,stoppa,pararectus approach.Methods Systematic literature retrieval was carried out to obtain two-arm and one-arm researches on the treatment of acetabular fractures via any one of the three approaches before May 2017,from pubmed,embase,cochrane library Databases.Data extraction and quality evaluation of studies were performed by 3 investigators independently.A meta-analysis was performed by RevMan 5.3 and Meta-Analyst beta 3.13.Results Totally 22 low to moderate quality studies,including 7 two-arm and 15 one-arm were included.There were 980 patients,including 581 in ilioinguinal approach group,264 in stoppa approach group and 135 in pararectus approach group.Meta-analysis showed the rate of anatomic reduction in stoppa approach was higher than that in ilioinguinal approach (OR=0.58,95%CI:0.36-0.94,P=0.03),which in pararectus approach was higher than that in ilioinguinal approach (0R=2.95,95%CI:1.22-7.10,P=0.02).Compared to the ilioinguinal approach,the operation time in the Stoppa approach was shorter (MD=48.01,95%CI:17.08-78.95,P=0.002),there was no statistically significant difference between pararectus approach and ilioinguinal approach.In addition,there were no statistically significant differences among three approaches in intra-operative blood loss,postoperative functional outcomes and complications.Conclusion The available limited evidence suggests that the rate of anatomical reduction in stoppa and pararectus approach may be higher than ilioinguinal approach.Compared to the ilioinguinal approach,the operation time in the stoppa approach may be shorter.In this field,further rigorous design,baseline parallel,direct comparison controlled studies on this topic are still needed.