The strain Streptomyces sp.,nominated IMB-14,was isolated from the soil sample of WuDang Mountain by the method of cellulose ester membrane filter.The studies on antibiotic activities,morphological characteristics,cultural characteristics,physiological characteristics,16S rDNA sequence analysis and the metabolite of strain IMB-14 showed that the strain IMB-14 was accordance with Streptomyces xanthoci-dicus.The study on the isolation and identification of strain Streptomyces xanthocidicus establishes a foundation on screening of novel antibacterial and antitumor agents.

Central serous chorioretinopathy (CSC) is characterized by serous detachment of the sensory retina as a consequence of the focal leakage of fluid from the choriocapillaries to subretinal space through a defect of the retinal pigment epithelium(RPE). The exact cause of CSC is not well unknown. Psychological stress is thought to contribute to CSC, but the physiologic mechanisms are unclear. It is hypothesized that psychological stress can induce CSC through the mechanism of the hypothalamic-pituitary-adrenal (HPA) system. Psychological stress can adversely affect HPA axis and causes glucocorticoid levels to elevate. Increased glucocorticoids constrict choroid vessels, which leads to ischemia of choroids and damage vascular endothelial cells, thus causing vasopermeability to increase. RPE dysfunction will occur as a result of abnormalities in the choroidal circulation. The large molecules including protein may enter the subretinal space through the damaged vessels and RPE.

BACKGROUND:Many studies have shown that Chinese medicineFuming granules have certain curative effects on optic nerve injury, but a large number of experimental studies are stil needed to verify. OBJECTIVE:To verify the effect of Chinese medicineFuming granules on optic nerve injury by the method of stress relaxation experiments. METHODS: The animal models of optic nerve injury were established by the clamping method. Rats were intervened withFuming granules by intragastric administration. Model group and normal control group were set for comparison. After 30 days of successive administration, optic nerve injury received stress relaxation experiments in each group, and histomorphology was observed. RESULTS AND CONCLUSION:In the normal control group, the optic nerve nuclei distributed uniformly without edema, augmentation or inflammatory cels, and the axon and other contents had a clear structure. In the model group, the optic nerve fiber arranged sparsely, presented unclear structure, and axon, karyorrhexis and the other contents changed. In theFuming granule group, the transect of optic nerve which arranged densely had large area, and most of the axon had a normal structure. The decreases in stress at 7 200 s and stress relaxation were as folows: normal control group > fuming granule group > model group (P < 0.05). Results confirmed that Chinese medicineFuming granule accelerates the recovery of axoplasm of injured optic nerve, restores the morphology of axons, and contributes to the recovery of injured optic nerve.

Objective To analyze the relationship between life style and chronic diseases.Method 2 886 people were selected randomly from three districts of Tianjin and surveyed with a questionnaire on their healthy condition and physical examination.2 622 people were taken a diet survey by 3-days foods intakes weighing and 24-hours diet recall,then the relative risk factors between life style and coronary heart disease,cerebrovascular disease,diabetes,hypertension,overweight and obesity were analyzed.Result The standardized prevalence rates of coronary heart disease,cerebrevascular disease,diabetes,hypertension, overweight and obesity of residents were 9.24%,4.33%,6.74%,34.05%,and 42.62%,respectively.The more the intake of energy, the more the risk for people to suffer from overweight and obesity,OR value being 1.00-1.70.With intake of more than 20 g dietary fiber per day,the risk for suffering from overweight and obesity could be reduced,OR value being 0.68.Logistic analysis showed that heavy salty taste could increase the risk for suffering from hypertension by 42%.Watching TV more than 3 hours per day increased the risk for suffering from cerebrovascular disease and diabetes,OR value being 1.802 and 1.703 respectively.Sleep less than 7 hours per day was a dangerous factor for hypertension,coronary heart disease and cerebrovasular disease,OR value being 1.517, 1.833 and 2.041 respectively.Conclusion The life style such as dietary pattern,sedentary occupation and sleep hours have strong connection with chronic diseases,such as hypertension,diabetes etc.Reasonable diet,suitable exercises,reducing sedentary position and increasing sleep hours will be good for health,and also for the prevention and control of chronic diseases.

Cadmium ; adverse effects ; Dose-Response Relationship, Drug ; Environmental Exposure ; adverse effects ; Humans ; Occupational Exposure ; adverse effects ; Risk Assessment

Cadmium ; pharmacology ; Cells, Cultured ; Heat-Shock Proteins ; biosynthesis ; Humans ; Metallothionein ; biosynthesis ; Ubiquitin ; biosynthesis

Objective To determine postoperative resting energy expenditure ( REE) in children with congenital heart disease ( CHD) requiring open heart surgery , to compare measured resting energy expenditure (MREE) with current predictive equations (PEE), and to investigate the possible influencing factors on the metabolic status of the postoperative CHD children .Methods From February 2015 to June 2015 , 150 ventila-ted children admitted to the cardiac intensive care unit after surgery for congenital heart disease in Shanghai Children's Medical Center were enrolled consecutively .Indirect calorimetry ( IC) measurements were performed using metabolic cart 4 hours after surgery .General clinical data were recorded .Results Totally 104 male and 46 female patients were enrolled, with a median age of 14 months (8.3-36.0 months).The non-protein re-spiratory quotient of patient was 0.79 ±0.20, MREE was (264.76 ±61.74) kJ/(kg· d), and PEE using Schofield equations was (278.51 ±93.42) kJ/(kg· d).Although there was no significant differences (P=0.096 ) between MREE and PEE , the agreement was poor between them ( R2 =0.119 ) .Multivariate stepwise regression analysis showed that MREE had significant positive correlation with risk adjustment in congenital heart surgery (RACHS-1) score (P=0.012) and negative correlation with age (P=0.010).Up to 97.33%of children (146/150) had lower energy intake compared with MREE on the 1st postoperative day .Conclu-sions MREE does not increase after surgery in CHD children;however , the substrate utilization is influenced . Factors influencing the postoperative REE include RACHS-1 score and age.The energy intake at 1st day after surgery is generally lower than REE in these children .

Background Fibrin glue has been utilized to adhere the graft during the pterygium excision with conjunctival autografts.Several relevant clinical randomized controlled trial(RCT) and retrospective studies have been published abroad,but the samples for its effectiveness and safety issue of fibrin glue and suture are still underinvestigation.Objective Current study was to quantificationally assess the efficacy and safety of fibrin glue versus sutures in the application of pterygium excision with conjunctival autografts.Methods Based on established search strategy,a computerized literature search was conducted to identify all citations concerning the RCT for effectiveness and safety evaluation of fibrin glue and suture for the graft fixation during the pterygium excision with conjunctival autografts from MEDLINE ( from 2000 to October,2010 ),EMbase ( from 2000 to October,2010 ),Cochrane Central Register of Controlled Trials ( Issue 4,2010 ),CBMdisc ( 2000 to October 2010 ),CNKI ( 2000 to October 2010 ),and the relevant conference proceedings and references searched by hand was performed as supplement.The included literature was scored with Jadad table.The Cochrane Collaboration' s RevMan 5.0 software was used for the test of heterogeneity or test for overall effect.The effective indexes,such as operative duration,recurrence rate and complication,were evaluated by Meta analysis.Results Six RCTs involving 401 eyes of 377 participants were identified.These literatures were published with English in 2004-2010 from China,New Zealand,Sweden,Israel,Turkey and Malasia and the Jadad scores were 4-5.The quantitatively analysis revealed that fibrin glue appeared to short the operative time compared with suturing method (MD =14.23 ;95％ CI:- 16.18- 12.29;P=0.00) and drop the rate of recurrence ( RR =0.49,95％ CI:0.26 -0.95 ; P =0.03 ).No significant differences were found in the rate of postoperative graft dehiscence or absence (RR =3.41,95 ％ CI:0.85-13.68;P =0.08 ).Conclusions Fibrin glue shows the good effectiveness and easy application during the pterygium excision with conjunctival autografts.Long-term follow-up of multi-central RCTs with a larger number of cases are still needed to support this conclusion.

To evaluate the clinical effect of laser in situ keratomileusis ( LASlK) with the corneal flap created by FEMTO LDV femtosecond laser for myopia.
●METHODS: The corneal flap was created by the FEMTO LDV femtosecond laser, and the thickness of the flap was 110μ m. A total of 143 myopic patients (283 eyes) were treated with the EC5000 - CXlll element laser. The optometry of the eye, best corrected visual acuity (BCVA) the thickness of the cornea, and ObscanⅡ were examined before the operation. The thickness of the flap was calculated by measuring the thickness of corneal bed during the operation in 35 eyes. The conditions of the corneal flap, complications, uncorrected visual acuity ( UCVA ), diopter, corneal topography were observed during and after the operation and were checked for 3mo follow-up.
●RESULTS: During the operation, it appeared small flap ( diameter < 5mm ) in 3 eyes, corneal margin incised incompletely in 5 eyes and incision bleeding in 8 eyes. Postoperative subconjunctival hemorrhage appeared in 6 eyes. The thickness of corneal flap in 35 eyes was 108. 75± 8. 52μ m (98-117μ m) and the error was 6. 49±8. 62μ m (3-12μ m). There was no significant difference between the actual flap thickness and the preset flap thickness ( P >0. 05) . The average equivalent spherical refractive was -0. 29± 0. 47 ( - 1. 50 to + 1. 00) DS after the operation for 3mo and the UCVA met or exceeded preoperative BCVA in 251 eyes (88. 7%).
●CONCLUSlON: The operation of myopia by LASlK flap created by FEMTO LDV femtosecond laser has fewer complications, and the effect is definite and safe.

Administration, Inhalation ; Bronchiolitis ; drug therapy ; Bronchodilator Agents ; administration & dosage ; therapeutic use ; Budesonide ; administration & dosage ; therapeutic use ; Female ; Humans ; Infant ; Male ; Poly I-C ; administration & dosage ; therapeutic use