Fungi ; Humans ; Rhinitis ; microbiology ; Sinusitis ; microbiology

Humans ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; Rhinitis, Allergic, Seasonal

Chronic Disease ; Humans ; Sinusitis ; therapy ; Treatment Outcome

Purpose To explore the role of scanning laser tomography in the assessment of macular hole surgery. Methods Fifteen eyes of 14 patients with macular holes underwent scanning of their affected macular area using the Heidelberg retina tomograph (HRT). The significance of topographic changes postoperatively were determined in eleven eyes which received vitrectomy surgery. The scan field was set at 15? of the retina and the depth was set to 1.5 mm or 2.0 mm. All the measurements were taken for 3 times and the average value of the 3 measurements was used. Results The average hole area was (0.499?0.34) mm 2 and the maximal depth of the hole was (0.284?0.11) mm. Topographic difference analysis of the eleven eyes showed a significant reduction in the height of the retina after vitrectomy. The maximal depth of the hole was (0.063?0.04) mm postoperatively. Conclusion Scanning laser tomography provides an objective evaluation of the anatomic outcome of the macular hole surgery.

Acupuncture Points ; Acupuncture, Ear ; Adult ; Dizziness ; therapy ; Headache ; therapy ; Humans ; Male ; Middle Aged ; Moxibustion ; Rhinitis, Allergic, Seasonal ; therapy

Bronchitis ; diagnosis ; Diagnostic Errors ; Humans ; Infant ; Male

Objective To study the effects of cool restrain stress on the accumulation of eosinophils and expression of Th cytokines in rat nasal mucosa with allergic rhinitis model.Methods Fifty healthy female rats were randomly divided into 5 groups:control group,allergic rhinitis(AR)group,AR plus stress group,stress plus AR group and simultaneous stress-AR group.Cool restrain stress,AR model and simultaneous stress-AR were made.Nasal mucosa of septum from rats of five groups were stained routinely by haematoxylin eosin(HE)and immunohistochemistry respectively.The density of eosinophils and expression of interleukin(IL)2,IL-6 were observed by using software of image analysis systems under microscope.Results The density of eosinophiles and IL-6 in the nasal mucosa of stress-AR group were significantly higher than those in AR[(14.1 ± 3.2)for eosinophiles,and(15.3 ± 4.8)for IL-6]and were also significantly higher than those in control groups[(2.3 ±1.4)for eosinophiles,and(4.9 ±2.4)for IL-6)],and the differences reached statistical significance.(F were respectively 7.06,7.14,8.54,8.20,P were respectively＜0.05 or＜0.01),but no significant differences of the three groups(AR plus stress,stress plus AR and simultaneous stress-AR groups)were found(F were respectivel 2.90 and 3.20,P＞0.05).The expression of IL-2 in nasal mucosa of stress-AR group was significantly reduced compared with AR and control groups(F were respectively 7.27,7.32,P were respectively＜0.05 or＜0.01).But there were also no significant differences of the three groups(AR plus stress,stress plus AR and simultaneous stress-AR groups,F=3.12,P＞0.05).Conclusions The abnormal infiltration and accumulation of eosinophiles and the differences in expression of IL-2 and IL-6 which represented Th1 and Th2 cytokines in rats nasal mucosa varied in different groups.The eosinophiles and IL-6 were rarely expressed in control group and moderately expressed in AR group,but significantly expressed in cool restrain groups.The IL-2 representing Th1 cytokines were reduced in cool restrain stress gruops.All these results indicated that cool restrain stress might play a role in inducing rat allergic rhinitis.

OBJECTIVETo investigate the relationship between expression of angiogenic factors and invasion and proliferation of pancreatic cancer cell.

METHODSThe three pancreatic cancer cell lines of SW1990, Panc-1 and PCT-3 were divided into four groups respectively: control group, VEGF siRNA group, bFGF siRNA group and VEGF siRNA + bFGF siRNA group. The expression and the secretion of VEGF and bFGF in the three cell lines were inhabited by VEGF siRNA and bFGF siRNA. The proliferation and the invasion of the three cell lines were determined by CCK-8 and Boyden Chamber invasion tests.

RESULTSExpressions of VEGF and bFGF in three cell lines were significantly inhibited by VEGF siRNA and bFGF siRNA. The proliferation was inhabited by VEGF siRNA and bFGF siRNA in SW1990 and Panc-1 (P < 0.05), while was not in PCT-3 (P > 0.05). The invasion was inhabited significantly by VEGF siRNA and bFGF siRNA in the three cell lines (P < 0.05). Combination of VEGF siRNA and bFGF siRNA resulted in more efficient influence in inhibition of invasion in Panc-1 and proliferation in PCT-3 and SW1990 than VEGF siRNA or bFGF siRNA individually.

CONCLUSIONThe decreased expression of VEGF and bFGF can inhabited the ability of invasion and proliferation of pancreatic cancer cell.

Cell Line, Tumor ; Cell Proliferation ; Fibroblast Growth Factor 2 ; genetics ; Humans ; Neoplasm Invasiveness ; Pancreatic Neoplasms ; pathology ; RNA, Small Interfering ; genetics ; Vascular Endothelial Growth Factor A ; genetics

Objective Therapeutic hypothermia has become a standard neuroprotective treatment in term newborn infants following perinatal asphyxia,but active cooling with whole body surface or head cooling is both complex and expensive.The clinical feasibility of passive cooling in treatment of full term infants with severe asphyxia was investigated.Methods Thirty-two severe asphyxiated term newborns treated with hypothermia were analyzed,who were randomly divided into 2 groups:passive cooling group(n =17) and active cooling group(n =15).Active cooling group adopted MTRE ALLONTM-thermo regulatory systems,passive cooling group using natural cooling method.Hypothermia treatment time was 72 hours.At the end of treatment,the clinical manifestations,biochemical parameters and clinical efficacy of infants between the 2 groups were compared.Results During treatment all infants had no cardiac arrhythmia,hypoglycemia,sustained metabolic acidosis,blood-borne infections,local cold injury or bleeding.Infants in passive cooling group had a relatively wide range of rectal temperature fluctuations[average (33.47-0.71) ℃] and infants in active cooling group had a relatively narrow range of rectal temperature fluctuations[average (33.66 ± 0.29) ℃],but there was no statistically significant difference in their mean rectal temperature(t =1.941,P =0.055).One patient died in active cooling group,but there were no significant differences in suckling age,length of hospital stay,neonatal behavioral neurological assessment score,abnormal cranial ultrasound and MRI between the survivors of the two groups(all P ＞0.05).Conclusions In NICU,environmental temperature is relatively stable,passive cooling for asphyxiated newborns appears to be feasible for maintenance of hypothermia with a lower risk of adverse reactions.

Hemangioendothelioma, Epithelioid ; Humans ; Male ; Maxillary Sinus Neoplasms ; Middle Aged