1.A Clinical Investigation on 10 Patients of Tuberous Sclerosis.
Hee Jung CHUNG ; Myung Jin KIM ; Chang Jun COE
Journal of the Korean Pediatric Society 1983;26(4):373-379
No abstract available.
Humans
;
Tuberous Sclerosis*
2.Regulation of Human Beta-Defensin 3(hBD-3) in Human Keratinocyte(HaCaT) Cell Lines.
Yu Jin KIM ; Chang Kwun HONG ; Seong Jun SEO
Annals of Dermatology 2003;15(1):1-7
BACKGROUND: The large surfaces of the skin are often initial site of contact between microorganism and human. The skin are coated with epidermis and epithelial cells can recognize microorganism and mount a fast defense through the production of various inducible antibiotic peptides. This leads to chracteristic broad spectrum of antimicrobial activity against bacteria, fungi, and viruses. Recent studies introduce us new peptides with antimicrobial activity such as P,-defensins and cathelicidins. They are expressed on the epithelia and polymorphonuclear leukocytes, which are first lines of defence from various invasive environments. Futhermore, they are considered very interesting and important endogenous antibiotics. Our previous study has shown that the expression of human defensin(hBD-2) mRNA, which is potent antibiotic peptide against Gram-negative bacteria(P. aeruginosa), was upregulated with ultraviolet(UV) irradiation, tumor necrosis factor-α(TNF-α) and lipopolysaccharide(LPS) in HaCaT cells. A novel hBD-3, 5-kDa, nonhemolytic antimicrobial peptide, was demonstrated a salt-insensitive broad spectrum of potent antimicrobial activity against many potentially pathogenic microbes in especially, multiresistant S. aureus. We have analyzed the expression patterns of hBD-3 in HaCaT cell lines. OBJECTIVE: This research have done in order to evaluate the expression and regulation of hBD-3 mRNA in human keratinocyte cell lines. METHODS: HaCaT cell lines were used to all culture experiments. Cultured human keratinocytes were stimulated with UV irradiation or TNF-α or LPS to determine whether hBD-3 mRNA production occurred. Reverse transcription-polymerase chain reaction (RT-PCR) was per-formed to amplify hBD-3 cDNA from stimulated keratinocytes in a time dependant manner, and densitometry was used to verify the specificity of RT-PCR amplication products. RESULTS: Expression of hBD-3 was upregulated with UV irradiation, TNF-α and LPS in Ha-CaT cells compared to control CONCLUSIONS: Human keratinocytes are capable to induce hBD-3 mRNA, as well as hBD-2, in response to UV irradiation, TNF-α and LPS. suggesting that these cells could play an important role against the bacterial infection and UV light damage in human skin.
Anti-Bacterial Agents
;
Bacteria
;
Bacterial Infections
;
Cathelicidins
;
Cell Line*
;
Densitometry
;
DNA, Complementary
;
Epidermis
;
Epithelial Cells
;
Fungi
;
Humans*
;
Keratinocytes
;
Necrosis
;
Neutrophils
;
Peptides
;
RNA, Messenger
;
Sensitivity and Specificity
;
Skin
;
Ultraviolet Rays
3.A Case of Phenylketonuria.
Ki Suk BAHN ; Jin Yong LEE ; Chang Jun COE
Journal of the Korean Pediatric Society 1985;28(1):99-102
No abstract available.
Phenylketonurias*
4.A Case of Silver Russel Syndrome.
Kee Hyuck KIM ; Chang Jun COE ; Duk Jin YUN
Journal of the Korean Pediatric Society 1986;29(3):86-89
No abstract available.
Silver*
6.A Case of Recurrent Bacterial Meningitis with CSF Rhinorrhea.
Dae Shik KIM ; Jin Yong LEE ; Chang Jun COE ; Jin Suk SUH
Journal of the Korean Pediatric Society 1989;32(8):1161-1166
No abstract available.
Meningitis, Bacterial*
8.A study for mass screening of galactosemia using galactitol level by spot urine method among Korean infants.
Jin Tae KIM ; Chong Won BAE ; Sa Jun CHUNG ; Chang Il AHN
Journal of the Korean Pediatric Society 1991;34(7):949-958
No abstract available.
Galactitol*
;
Galactosemias*
;
Humans
;
Infant*
;
Mass Screening*
9.Nitroblue Tetrazilium dye Reduction Test for Differential Diagnosis of Varilus Menigitis.
Hong Kyu LEE ; Chang Jun COE ; Duk Jin YUN
Journal of the Korean Pediatric Society 1978;21(4):290-295
The usefullness of the nitroblue tetrazolium (NBT) test as an aid in the differential diagnosis of various meningitis was evaluated in infancy and childhood. 36 cases of patients with various meningitis who were admitted to the department of pediatrics in Severance Hospital from June, 1975 to March, 1976 and 8 cases of healthy children as control were the subject of this study. The following results were observed; the mean proportion of NBT-positive neutrophils was 4.0?2.0% in healthy control group, 17.0?.7% in patients with bacterial meningitis, 6.0?.3% in tuberculous menigitis, and 5.o?,1% in aspetic meningitis. And we couclued that NBT test is useful in differenciating various form of meningitis.
Child
;
Diagnosis, Differential*
;
Humans
;
Meningitis
;
Meningitis, Bacterial
;
Neutrophils
;
Nitroblue Tetrazolium
;
Pediatrics
10.Clinical Obseervation and Changing Patterns of Resistance to Antibiotics in Childhood Shigellosis.
Young Mo SOHN ; Kwand Ho KIM ; Chang Jun COE ; Pyung Kil KIM ; Duk Jin YUN
Journal of the Korean Pediatric Society 1981;24(3):229-234
No abstract available.
Anti-Bacterial Agents*
;
Dysentery, Bacillary*