3.The Effects of Oxalate on the DNA Synthesis in LLC-PK1 Cells.
Byong Chang JUNG ; Hyeon Hoe KIM ; Si Whang KIM
Korean Journal of Urology 2000;41(4):512-515
No abstract available.
Animals
;
DNA*
;
LLC-PK1 Cells*
;
Swine
4.Analysis of 35 cases of microsurgical resection and anstomosis in the management of the patjologic tubal occlusion.
Noh Hyeon PARK ; Young Chul YOO ; Chang Jae SHIN ; Jung Gu KIM ; Yoon Seok CHANG
Korean Journal of Obstetrics and Gynecology 1991;34(5):739-746
No abstract available.
Sterilization, Tubal*
5.Molecular Epidemiology of Fecal Oxalobacter formigenes in Healthy Adults Living in Seoul, Using a Polymerase Chain Reaction-Based Detection System.
Byong Chang JUNG ; Cheol KWAK ; Hee Kyung KIM ; Eui Chong KIM ; Hyeon Hoe KIM
Korean Journal of Urology 2000;41(12):1540-1545
No abstract available.
Adult*
;
Humans
;
Molecular Epidemiology*
;
Oxalobacter formigenes*
;
Seoul*
6.Ameloblastic fibro-odontoma in the mandible: a case report.
Korean Journal of Oral and Maxillofacial Radiology 2005;35(1):55-58
Ameloblastic fibro-odontoma is a rare benign mixed odontogenic tumor with histologic characteristics of ameloblastic fibroma and complex odontoma. As with ameloblastic fibroma, it may be asymptomatic or found because of painless swelling and delayed eruption of associated tooth. This report presents a case of ameloblastic fibro-odontoma in the posterior mandible of a 14-year-old girl and analyses its clinical features and radiographic features on plain X-ray film and CT images.
Adolescent
;
Ameloblasts*
;
Female
;
Fibroma
;
Humans
;
Mandible*
;
Odontogenic Tumors
;
Odontoma*
;
Tooth
;
X-Ray Film
7.Low-attenuation mediastinal masses on CT.
Hee Suk LEE ; In Joo CHEONG ; Seung Hyeon KIM ; Shin Hyung LEE ; Chang Joon LEE
Journal of the Korean Radiological Society 1991;27(5):647-655
No abstract available.
8.Molecular Cloning and Nucleotide Sequence of the Gene Encoding Fusion(F) Protein of the Thermostable Newcastle Disease Virus Isolated from a Diseased Pheasant.
Kyung Soo CHANG ; Kui Hyun KIM ; Moo Hyung JUN ; Hee Jong SONG ; Jong Hyeon PARK
Journal of the Korean Society of Virology 1998;28(3):233-244
The gene encoding F protein of CBP-1 strain, a heat-stable Newcastle disease virus (NDV) isolated from the diseased pheasants in Korea, was characterized by reverse transcription-polymerase chain reaction (RT-PCR), nucleotide and amino acid sequences. Virus RNA was prepared from the chorioallatoic fluid infected with NDV CBP-1 virus and cDNA was amplified by RT-PCR, cloned and sequenced to analyze. The PCR was sensitive as to detect the virus titer above 25 hemagglutination unit. 1.7kb (1,707bp) size of the cDNA was amplified and cloned into BamHI site of pVL1393 Baculo transfer vector. The nucleotide sequences for F protein were determined by dye terminator cyclic sequencing using four pairs of primers, and 553 amino acid sequences were predicted. In comparison of the nucleotide sequence of F gene of CBP-1 with those of other NDV strains, the homology revealed 88.8%, 98.5% and 98.7% with Kyojungwon (KJW), Texas GB and Beaudette C strains, respectively. As the deduced 553 amino acid sequences of F protein of CBP-1 were compared with those of other NDV strains, the homology appeared 89.9%, 98.7% and 98.9% with KJW, Texas GB and Beaudette C strains, respectively. The putative protease cleavage site (112-116) was R-R-Q-K-R, indicating that CBP-1 strain is velogenic type. The amino acid sequences include 6 sites of N-asparagine-linked glycosylation and 13 cysteine residues. These data indicate that the genotype of CBP-1 strain is more closely associated with the strains of Texas GB and Beaudette C than KJW strain.
Amino Acid Sequence
;
Animals
;
Base Sequence*
;
Clone Cells
;
Cloning, Molecular*
;
Cysteine
;
DNA, Complementary
;
Genotype
;
Glycosylation
;
Hemagglutination
;
Korea
;
Newcastle disease virus*
;
Newcastle Disease*
;
Polymerase Chain Reaction
;
RNA
;
Texas
;
Viral Load
9.A Study of Partial Excision and Suvdermal Exicision in Surgical Treatment of Axillary Osmidrosis.
Young Dae KWEON ; Jin Gyu LEE ; Hyeon Ho SEO ; Chang Sik KIM ; Ji Woon HA
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1999;26(5):816-821
There are 3 basic methods for surgical treatment of axillary osmidrosis; 1) method that removes only subcutaneous cellular tissue without removing skin 2) method that removes skin and subcutaneous cellular tissue en bloc, and 3) method that partially removes skin and subcutaneous cellular en bloc as well as removing the subcutaneous cellular tissue of the adjacent region. We studied the results of partial removal of the skin and subcutaneous cellular tissue en bloc, as well as the removal of subcutaneous cellular tissue of the adjacent region to compare the results of the bipedicled flap with the graft conversion method. There was no difference between two methods in results and complication rates. There are 3 advantage to this procedure. First, about 70-80% of apocrine glands were centrally distributed among the axillary hairbearing region therefore, resection of the central portion of axillary hair distribution area is important for good result. Second, the preservation of the subdermal plexus with careful excision of adjacent underlying subcutaneous tissue under the aid of the magnifying surgical loupe, is important for good wound healing. Third, the central excision of the axillary hair distribution area provides good exploration for undermining and defatting of the undersurface of the adjacent area, therefore it tooks a shorter operation time.
Apocrine Glands
;
Hair
;
Skin
;
Subcutaneous Tissue
;
Transplants
;
Wound Healing
10.Detection of BLV Proviral DNA in Korean Native Goats Experimentally Infected with Bovine Leukemia Virus by Polymerase Chain Reaction.
Moo Hyung JUN ; Kyung Soo CHANG ; Young Sung CHO ; Jong Hyeon PARK ; Soo Hwan AN
Journal of the Korean Society of Virology 1997;27(2):217-226
PCR implication using the primers for gag, pol and rev genes in BLV (bovine leukemia virus) proviral DNA and syncytium assay were carried out for the Korean native goats experimentally infected with bovine leukemia virus to investigate pathogenesis of BLV in the goats, and to establish a model animal for BLV infection. The oligonucleotide primers used in PCR revealed very high specificity, The minimal amount of FLK-BLV cellular chromosomal DNA to detect the integrated BLV proviral DNA was 10 ng. The peripheral blood lymphocytes from the goat infected with BLV were examined at regular intervals by PCR amplification and syncytium assay. Pol or gag genes were detected in none of three infected goats at the 1st week post-infection (p.i.). At the 4th week p.i., one of three goats showed the amplified gag gene. Thereafter detection rates for the genes were increased, indicating that the BLV proviral genes were integrated in all of the lymphocytes from three goats, at the 16th weeks p.i., when it was evident in syncytium assay that the lymphocytes from all of three goats were infested with infective BLV. Investigating the tissues from the necropsied goats at the 8th month p.i., the amplified BLV proviral genes and infective BLV were detected in all of the peripheral lymphocytes from three infected-goats. Among various tissues examined, the amplified BLV proviral genes were observed in spleen and superficial cervical, mandibular and retropharyngeal lymph nodes, and the infective BLV, in superficial cervical and mandibular lymph nodes. It was assumed that the Korean native goat was quite susceptible to BLV infection, indicating that the goat could be a good model animal for BLV.
Animals
;
Cattle
;
Deltaretrovirus Infections
;
DNA Primers
;
DNA*
;
Enzootic Bovine Leukosis*
;
Genes, gag
;
Genes, rev
;
Giant Cells
;
Goats*
;
Leukemia
;
Leukemia Virus, Bovine*
;
Lymph Nodes
;
Lymphocytes
;
Polymerase Chain Reaction*
;
Sensitivity and Specificity
;
Spleen