Adolescent ; Adult ; Colic ; chemically induced ; nursing ; Female ; Humans ; Lead Poisoning ; nursing ; Male ; Middle Aged ; Young Adult

Objective To investigate the roles of differentiation and proliferation related proteins in neural stem cells (NSCs) in rats treated with L monosodium glutamate (MSG). Methods Tissue proteins were extracted from the rat forebrain. Western blotting was used to investigate the changes of differentiation and proliferation related proteins in NSCs in rats treated with MSG. Results Compared with those in rats of the normal control group, the related proteins (Notch1, hes5, Mash1, and NeuroD) in NSCs were significantly decreased in MSG rats at 30 and 60 d, especially for Notch1 and hes5 ( P

Objective To explore the changes of differentiation and proliferation of neural stem cells in rats treated with corticosterone (CORT). Methods Adult SD rats were administered with CORT (10 mg?kg -1 ?d -1 ) by consecutive subcutaneous injection for 14 days. Single and double immunohistochemical stainings were used to identify the neural stem cells and differentiated neural cells in adult rats. Results Compared with that in the normal control group, the number of BrdU immunohistofluorescence positive cells and BrdU+NF200, BrdU+NeuroD, BrdU+GFAP double immunostaining positive cells in the cerebral cortex, hippocampus, and hypothalamus were markedly decreased ( P

The pathogenesis of cancer and related treatment were discussed.With regard to cancer,WANG emphasized the vital qi tangled theory,evil dominant theory,theory of blood stasis resulting tumor,non-blood stasis theory,sputum and toxin harm theory.As for cancer treatment,method of expelling stagnation,regulating qi,eliminating phlegm,softening hard mass and detoxicating should be used according to the specific syndrome.

Objective To investigate the relation between expression of MMP-9 and RECK in laryngeal carcinoma and it′s clinical characteristics and to explore the biological action of laryngeal tumor. Methods 38 laryngeal squamous cell carcinoma (LSCC)specimens and normal mucosa were examined for the expression level of MMP-9 and RECK protein by IHC method ( PV9000) and RT-PCR was employed to measure the expression of MMP-9 and RECK mRNA. Ten laryngeal papilloma were used for controls. Results The expression level of MMP-9 protein in carcinoma of larynx was 92.11%,which was significantly higher than that of the adjacent tissues (P

Objective To investigate the incidence of leukemia in infants, and to understand the status of this disease in recent years. Methods A total of 5802 children aged median 6 years (0-12 years) with first time bone marrow biopsy from January 2009 to December 2015 were retrospectively analyzed, and the results of bone marrow image analysis were analyzed retrospectively to investigate the incidence of leukemia. Results Of the 5802 cases, 480 (8.27 %) cases were children with leukemia. Among them, 381 (79.38 %) cases were acute lymphocytic leukemia (ALL), 99 (20.62 %) cases were acute myeloid leukemia (AML). The proportion of children with leukemia with first time marrow puncture was 5.33%(40/750), 4.92%(36/731), 7.06%(58/821), 8.34%(78/935), 10.13%(88/868), 10.89%(92/845), and 10.33%(88/852) from 2009 to 2015, respectively. The proportion of male patients per year was more than female, and the incidence in preschool and school-age children was higher [33.75 % (162/480) and 34.79 % (167/480), respectively]. Conclusion The proportion of children's leukemia in children with the first time bone marrow aspiration in Shanxi Children's Hospital increases year by year, and its risk factors need to be further analyzed.

We studied the protective effects of redix salviae miltiorrhizae (RSM) on the ischemic and post-ischemic reperfusion injury which was made by ligating and reopening of the left ventricular branch of the coronary artery of the open-chest anaethetised rabbits under the supervision of ECG. There were 14 rabbits in each group, 7 of them for the measurement of myocardial lipid peroxide level (MLPL) by the thiobarbituric acid method (Hiroshi Ohkawa, 1979), and the other 7 for measuring distribution of regional myocardial blood flow (RMBF) by the biological radionuclidelabeled particle method (Wang Jingxian, 1985). With increasing duration of ischemia, MLPL increased significantly in the ischemic and post-ischemic reperfusion injured heart. In the case of 60 mins ischemia followed by 30 mins reperfusion, MLPL was much higher than that without reperfusion. However, which compared with that of 90 mins ischemia, no significant difference was found. The recovery of RMBF was only 53.2% after 30 mins of reperfusion following 60 mins of ischemia. The occurance of post-ischemic reperfusion injury was dependent on the duration and severity of ischemia. The injury of reperfusion after long period (60 mins) of ischemia is similar to that of increasing duration of ischemia. No-reflow phenomenon was observed in ischemic zones during reperfusion after 60 mins of ischemia. In the RSM (2g/kg body wt) protected reperfusion group. MLPL was reduced by 56.0% and the recovery of RMBF was increased by 32.0%.

Micro-ribonucleic acids (miRNAs) are endogenous single-stranded small non-coding RNAs. miRNAs bind to a com-plementary site in the 3' untranslated region of their target mRNAs through canonical base pairing, which can direct the degradation or translational repression of these transcripts. Thus, miRNAs can effectively silence the protein expression of target genes post-transcrip-tionally. miRNAs may also regulate the expression of oncogenes and tumor suppressor genes and could be involved in almost all known hallmarks of cancinogenesis. In this paper, we discuss the following in detail:(1) biogenesis and main functions of cellular miR-NAs, (2) stability and detectability of exosomal miRNAs in biological fluids;and (3) feasibility of miRNAs as a potential new class of biomarkers derived from urinary exosome in the malignancy of urinary system. Finally, we summarize studies on urinary exosomal miRNAs as potential biomarkers of prostate, bladder, and kidney cancers.

The lymphatic vessel is one of the earliest routes for systemic neoplastic metastasis. But reports on research of the lymphatic vessel have been few compared to the study for tumor vessels. In the past few years,along with advances in understanding about the construction features of the lymphatic system,work has been done to determine the anatomic fundamentals of lymphatic metastasis. Many new specifi c markers of the lymphatic endothelium had been reported, which is useful to differentiate lymphatic vessels from capillary vessels. The lymphatic vessels are usually found in the animal tumor models, human tumors and peritumoral tissues. Furthermore, the molecular mechanism of lymph-angiogenesis has been discovered, which provide effective targets for anti-lymphatic metastasis of malignant tumors, make optimization of individualized treatment strategy possible to restrain lymph-angiogenesis of malignant tumors and so it could benefi t in improving patients' prognosis and survival.

Objective To study the application of ultrasonic freestyle extended imaging(FEI) in thyroid,breast,artery,vein diseases.Methods Thirty-five patients with above mentioned diseases were examined by FEI and the images were analyzed.Results FEI could entirely show the scan region needed.It also could enhance image quality,show the physiologic radian of the body,measure the nidi accurately.Conclusion The FEI technology provides more image information and is of great help to improve the clinic diagnosis ratios.