No abstract available.
Carcinoma, Renal Cell* ; Survival Rate*

No abstract available.
Animals ; Corrosion Casting* ; Corrosion* ; Head* ; Rats*

Thirty-six patients (24 with oral cavity or oropharynx cancers and 12 larynx cancers) were prospectively examined with computed tomography (CT) to determine its value in staging the primary tumor and to compare with the clinical staging. The CT staging agreed with the clinical staging in 50% (12/24) of the oral cavity or oropharynx cancers and in 67% (8/12) of the larynx cancers. The CT upgraded the clinical staging in 29% (7/24) of the oral cavity or oropharynx tumors and in 33% (4/12) of the larynx cancers, whereas the CT downgraded the clinical staging in 21 % (5/24) of the oral cavity or oropharynx cancers. There is no downgrade on CT in larynx cancer. The post-surgical confirmation was not made in most of the cases. The possible causes of disagreement between the CT and the clinical staging, and the diagnostic value of the CT in evaluation of the primary tumor in oral cavity, oropharynx and larynx were discussed.
Head and Neck Neoplasms ; Head ; Humans ; Laryngeal Neoplasms ; Larynx ; Mouth ; Oropharyngeal Neoplasms ; Oropharynx ; Prospective Studies

No abstract available.
Carcinoma, Renal Cell*

No abstract available.
Head and Neck Neoplasms* ; Head*

No abstract available.
Contrast Media* ; Magnetic Resonance Imaging*

No abstract available.
Humans ; Killer Cells, Natural* ; Lymphocytes* ; T-Lymphocyte Subsets* ; Uterine Cervical Neoplasms*

No abstract available.

OBJECTIVE: Recently, microdissection of tissue sections has been used increasingly for the isolation of morphologically identified homogeneous cell populations, thus overcoming the obstacle of tissue complexity for the analysis cell-specific expression of macromolecules. The aim of the present study was to establish the minimal conditions required for the RNA extraction and amplification from the cells captured by the laser captured microdissection. METHODS: Mouse ovaries were fixed and cut into serial sections (7 micrometer thickness). Oocytes were captured by laser captured microdissection (LCM) method by using PixCell IITM system. The frozen sections were fixed in 70% ethanol and stained with hematoxylin and eosin, while the paraffin sections were stained with Multiple stain. Sections were dehydrated in graded alcohols followed by xylene and air-dried for 20 min prior to LCM. All reactions were performed in ribonuclease free solutions to prevent RNA degradation. After LCM, total RNA extraction from the captured oocytes was performed using the guanidinium isothiocyanate (GITC) solution, and subsequently evaluated by reverse transcriptase -polymerase chain reaction (RT-PCR) for glyceraldehyde-3-phosphate-dehydrogenase (GAPDH). RESULTS: With the frozen sections, detection of the GAPDH mRNA expression in the number of captured 25 oocytes were not repeatable, but the expression was always detectable from 50 oocytes. With 25 oocytes, at least 27 PCR cycles were required, whereas with 50 oocytes, 21 cycles were enough to detect GAPDH expression. Amount of the primary cDNA required for RT-PCR was reduced down to at least 0.25 microl with 50 oocytes, thus the resting 19.75 microl cDNA can be used for the testing other interested gene expression. Tissue-to-slide, tissue-to-tissue forces were very high in the paraffin sections, thus the greater number of cell procurement was required than the frozen sections. CONCLUSION: We have described a method for analyzing gene expression at the RNA level with the homogeneously microdissected cells from the small amount of tissues with complexity. We found that LCM coupled with RT-PCR could detect housekeeping gene expression in 50 oocytes captured. This technique can be easily applied for the study of gene expression with the small amount of tissues.
Alcohols ; Animals ; DNA, Complementary ; Eosine Yellowish-(YS) ; Ethanol ; Female ; Frozen Sections ; Gene Expression* ; Genes, Essential ; Guanidine ; Hematoxylin ; Mice ; Microdissection* ; Oocytes* ; Ovary ; Paraffin ; Polymerase Chain Reaction ; Ribonucleases ; RNA Stability ; RNA* ; RNA, Messenger ; RNA-Directed DNA Polymerase ; Xylenes

This study is standard surgical measurement of the size of kidney in normal Korean population that might be basic data of renal size in comparison with radiographic and ultrasonographic measurement for clinical implies. The 235 cases of donors for renal transplantation were measured by the length, width, thickness, weight and the outer diameter of renal vessels as well as identification of branches of renal vein at the time of donor nephrectomy. These data were tested to statistically significant by T- test, ANOVA, Pearson correlation analysis and multivariate analysis. The mean values of renal size were 11.6+/-0.87 (9.2-15.4cm) x 6.1+/-0.81 (4.4-10.0cm) x 4.9+/-0.75(2.2-7.1cm) for male 11.7+/-0.77 (9.8-14.0cm) x 6.2+/-0.85 (4.5-10.0cm) x 5.O+/-0.77 (2.8- 7.1cm) and for female 11.4+/-0.96 (9.2-15.4cm) x 6.0+/-0.75 (4.4-8.2cm) x 4.8+/-0.7 (2.2-7.0cm). The mean value of renal weight were 183.1+/-36.92 (115.0-370.0gm) : for male 188.2+/-40.10 (120.0-370.0gm) and for female 177.6+/-32.41 (115.0-300.0gm). The width, thickness and weight of kidney were larger in male than in female (p<0.05). I found out that the age of donors did not affect the renal length, width, thickness and weight (p>0.05). The renal length, width, and weight increased proportional to the body weight (p<0.05). The renal thickness and weight were different in each range of the body height (P<0.05). The body weight had correlation with renal length (r=0.25), thickness (r=0.32), weight (r=0.36, p<0.001) and width (r=0.16, p<0.05). The body height was correlated well with renal length (r=0.20), thickness (r=0.18) and weight (r=0.25, p<0.05). The body weight was the most reliable factor affecting renal size and weight in multivariate analysis (p<0.05). The mean number of branches of renal vein was 0.36+/-0.63 (0.0-2.0) in right kidney and 3.12+/-0.76 (1.0-6.0) in left one. The mean diameter of the renal artery was 0.61+/-0.132 (0.3-1.0cm) and the mean diameter of the renal vein 1.46+/-0.132 (0.50-2.20cm). The radiographic measurement of renal size was larger than our surgical measurement. The mean renal length and width in radiographic measurement(IVP) were 12.1+/-0.70cm in right kidney, 12.1+/-0.65cm in left one and 6.4+/-0.54cm in right kidney, 6.8+/-0.45cm in left one versus 11.6+/-0.87cm, 11.6+/-0.89cm and 6.1+/-0.81cm, 6.1+/-0.84cm in surgical measurement. Surgical measurements of renal size of donors at the time of renal transplantation were real renal size despite of radiographic and ultrasonographic measurement and provided basic standard data for Korean population that might be utilized in approaching renal disease.
Adult* ; Body Height ; Body Weight ; Female ; Humans ; Kidney ; Kidney Transplantation ; Male ; Multivariate Analysis ; Nephrectomy ; Renal Artery ; Renal Veins ; Tissue Donors