1.A human infection of Echinostoma hortense in duodenal bulb diagnosed by endoscopy.
Young Doo CHANG ; Woon Mok SOHN ; Jae Hwa RYU ; Shin Yong KANG ; Sung Jong HONG
The Korean Journal of Parasitology 2005;43(2):57-60
As gastroduodenoscopy performed more frequently, case reports of human echinostomiasis are increasing in Korea. A Korean woman presented at a local clinic with complaints of abdominal pain and discomfort that had persisted for 2 weeks. Under gastroduodenoscopy, two motile flukes were found attached on the duodenal bulb, and retrieved with endoscopic forceps. She had history of eating raw frog meat. The two flukes were identified as Echinostoma hortense by egg morphology, 27 collar spines with 4 end-group spines, and surface ultrastructural characters. This report may prove frogs to be a source of human echinostome infections.
Animals
;
Duodenal Diseases/*parasitology
;
Duodenum/*parasitology
;
Echinostoma/*isolation & purification
;
Echinostomiasis/*diagnosis
;
Endoscopy
;
Female
;
Humans
;
Korea
;
Middle Aged
2.Torsion of Ovarian Tumor: CT Findings.
So Yong CHANG ; Eun Ju LEE ; Hee Sug RYU ; Jung Ho SUH
Journal of the Korean Radiological Society 1996;35(5):771-776
PURPOSE: To evaluate characteristic CT features of twisted ovarian tumors and to assess the diagnostic value of the existence of twisted vascular pedicle in torsion of ovarian tumors. MATERIALS AND METHODS: Weretrospectively reviewed the clinical and CT findings in eleven patients with ovarian tumor torsion confirmed bysurgery. Pre- and post-contrast CT were available in nine patients and post-contrast CT in two. All twisted ovaries, tubes, and tumors showed hemorrhagic infarction and necrosis. RESULTS: All patients had twisted vascular pedicle of the ovarian tumor evaluated by CT. Eight patients with left ovarian tumor had twisted vascular pedicleon the left side of the tumor, and three patients with right ovarian tumor on the right side of the tumor. Themorphologic characteristics of twisted vascular pedicle were coiled target appearance (4 cases), beak appearance(3 cases), doughnut appearance (3 cases), and mass with irregular margin (1 case). High density on precontrast CT scan was present in the pedicle in four cases and linear contrast enhancement was seen in the peripheral portionof the pedicle in four cases. The shape, density and location of ovarian tumors were inconsistent, however, contrast enhancement was not seen in all patients. Other findings were uterine deviation, loss of fat plane andpelvic fluid. CONCLUSION: Detection of twisted vascular pedicle by CT may be useful for the diagnosis of ovariantumor torsion.
Animals
;
Beak
;
Diagnosis
;
Humans
;
Infarction
;
Necrosis
3.Analysis of Mediatinal Lymph Nodes with Internal Low Density on Contrast Enhanced CT Scan.
Young Hoon RYU ; Kyu Ok CHOE ; Yong Kook HONG ; Sung Kyu KIM ; Joon CHANG ; Won Young LEE
Tuberculosis and Respiratory Diseases 1997;44(2):264-279
BACKGROUND: To analyze the morphologic characteristics of low density lymph node in etiologic differentiation of lymphadenopathy, emphasizing the different features between tuberculosis and lung cancer, on contrast enhanced CT scan,. METHOD: A total of 64 patients who showed low density lymph nodes on chest CT scan were analyzed. Primary causes were tuberculosis (n=28), lung cancer (n=27), malignant lymphoma (n=5) and metastasis from extrathoracic malignancies (n = 4). CT scan was performed with 10mm slice thickness and 7 characteristic features were evaluated: location,size, presence or absence of the nonnecrotic lymph node, calcification, perinodal fat obliteration, thickness and evenness of the enhancing rim. RESULTS: In patients with tuberculous lymphadenopathy, lymph nodes with uneven (68.0%) and thick (62.1%) enhancing rim were more common than lung cancer (p<0.05). Low density lymph nodes with less than 1cm in size were found only in tuberculous lymphadenopathy(n=10). In 48.2% of patients with lung cancer, more than 1 nonnecrotic enlarged lymph node were coexisted, whereas 21.4% in patients with tuberculous lymphadenopathy(p=0.06). However, the size, location and calcification were not statistically significant between tuberculous lymphadenopathy and lung cancer. CONCLUSION: Tuberculous lymphadenopathy is strongly suggested when enhancing rim of enlarged lymph nodes is uneven and thick, when the coexisting nonnecrotic lymph nodes are few in number and when central low density is encountered in normal sized lymph nodes.
Humans
;
Inflammation
;
Lung Neoplasms
;
Lymph Nodes*
;
Lymphatic Diseases
;
Lymphoma
;
Neoplasm Metastasis
;
Tomography, X-Ray Computed*
;
Tuberculosis
4.Proliferation Patterns of N-butyl-N- ( 4-hydroxybutyl ) Nitrosamine ( BBN ) Induced Rat Bladder Tumor Identified by Histone mRNA In Situ Hybridization.
Chang Ou RYU ; Jung Won SEO ; Yong Tae KIM ; Won Jae KIM
Korean Journal of Urology 1997;38(2):111-119
The synthesis of histone mRNA is closely coupled with DNA replication. During the S-phase of the cell cycle, the level of histone mRNA increases over fifty fold then rapidly disappears at the start of G2-phase. The presence, therefore, of abundant quantities of histone mRNA provides a molecular marker of cycling cells. The expression of histone mRNA was investigated by in situ hybridization method in N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) induced rat bladder tumor to determine the proliferation patterns. This method resulted in intense brown/black cytoplasmic staining of cells containing histone mRNA and extent and intensity of stain were increased in tumorigenesis (normal, simple hyperplasia, nodular or papillary hyperplasia, and transitional cell carcinoma-Ta,T1). The higher S-phase fraction indicated greater biological malignancy based on the fact that the extent and intensity of stain progressively increased with tumorigenesis.
Animals
;
Carcinogenesis
;
Cell Cycle
;
Cytoplasm
;
DNA Replication
;
Histones*
;
Hyperplasia
;
In Situ Hybridization*
;
Rats*
;
RNA, Messenger*
;
Urinary Bladder Neoplasms*
;
Urinary Bladder*
5.A case of liposarcoma of spermatic cord.
Dae Chang RYU ; Dong Seong KIM ; Hei Young SHIM ; Han Yong CHOI
Korean Journal of Urology 1992;33(3):564-566
Liposarcoma of the spermatic cord is a rare tumor. Most are of a low-grade malignancy and recurrences tend to be localized. Preoperative diagnosis is difficult. Wide local excision with radical orchiectomy appears to be adequate treatment. A regional lymph node dissection is usually not advocated since the pattern of spread is hematogenous. We report a case of liposarcoma of the spermatic cord in 49-year-old male with brief review of the literatures.
Diagnosis
;
Humans
;
Liposarcoma*
;
Lymph Node Excision
;
Male
;
Middle Aged
;
Orchiectomy
;
Recurrence
;
Spermatic Cord*
6.Usefulness of a developed four-channel EGG system with running spectrum analysis.
Deok Won KIM ; Chang Yong RYU ; Sang In LEE
Yonsei Medical Journal 2000;41(2):230-236
Electrogastrography (EGG) is a method of measuring action potentials on the abdomen. It is noninvasive, inexpensive and easy to measure. However, the EGG signal has a very low frequency (0.05 Hz) and an extremely low amplitude (10-100 microV). Consequently, its measured waveform is difficult to analyze and it is not yet completely understood. In this study, a four-channel EGG measurement system was built to measure the action potential of the stomach. This system was compared with the commercially available one-channel Digitrapper EGG. The 3 cpm percentages were compared between the best channel of the four-channel system and channel 1, whose electrode position was similar to the commercially available one-channel system for normal subjects. The average 3 cpm percentage of the best channel and channel 1 for preprandial measurement was 89.5% and 83.2%, respectively, and this was statistically significant (p < 0.001). Also the average 3 cpm percentage of the best channel and channel 1 for postprandial measurement was 90.4% and 76.5%, respectively, and this was statistically significant (p = 0.003). From these results, it can be concluded that a multi-channel EGG system is required for better EGG measurement.
Action Potentials*
;
Adult
;
Human
;
Stomach/physiology*
7.Effects of Reactive Oxygen Species on Sperm Function, Lipid Peroxidation and DNA Fragmentation in Bovine Spermatozoa.
Buom Yong RYU ; Yung Chai CHUNG ; Chang Keun KIM ; Hyun A SHIN ; Jung Ho HAN ; Myung Geol PANG ; Sun Kyung OH ; Seok Hyun KIM ; Shin Yong MOON
Korean Journal of Fertility and Sterility 2002;29(2):105-115
OBJECTIVE: To evaluate the effects of the reactive oxygen species (ROS) generated with a xanthine(X) and xanthine oxidase (XO) system on sperm function, the change of sperm characteristics, lipid peroxidation, and DNA fragmentation in bovine spermatozoa. MATERIALS AND METHODS: ROS were produced using a combination of 100 micrometer X and 50 mU/ml XO. The ROS scavengers: superoxide dismutase (SOD)(200mu/ml) and catalase (500mu/ml) were also tested. Spermatozoa were incubated for 2 hours in BWW medium with a combination of X-XO supplemented with or without ROS scavengers at 37degrees C under 5% CO2 incubator. Sperm movement characteristics by CASA (computer-aided sperm analysis), HOST (hypoosmotic swelling test), Ca-ionophore induced acrosome reaction, malondialdehyde formation for the analysis of lipid peroxidation, the percentage of DNA fragmentation using the method of TdT-mediated nick end labelling (TUNEL) by flow cytometry were determined after 2 hours incubation. RESULTS: The action of ROS on bovine spermatozoa resulted in a decreased in capacity for sperm motility, Ca-ionophore induced acrosome reaction and membrane integrity, an increased in malondialdehyde formation and the percentage of sperm with DNA fragmentation. In the effects of antioxidant, catalase completely alleviated the toxic effects induced by the ROS in terms of sperm function and characteristics, however SOD exhibited no capacity to reduce the toxic effects. CONCLUSION: The ROS can induce significant damages to sperm functions and characteristics. The useful ROS scavengers can minimized the defects of sperm function and various damages of spermatozoa.
Acrosome Reaction
;
Catalase
;
DNA Fragmentation*
;
DNA*
;
Flow Cytometry
;
Incubators
;
Lipid Peroxidation*
;
Malondialdehyde
;
Membranes
;
Reactive Oxygen Species*
;
Sperm Motility
;
Spermatozoa*
;
Superoxide Dismutase
;
Xanthine Oxidase
8.Endomyocardial Fibrosis: A Case Report.
Chang Hyu CHOI ; Yee Tae PARK ; Pil Won SEO ; Sung Sik PARK ; Myung Yong LEE ; Jae Wook RYU ; Gyung Min RYU ; Jae Hyun KIM ; Sam Hyun KIM
The Korean Journal of Thoracic and Cardiovascular Surgery 1999;32(3):310-314
Endomyocardial fibrosis(EMF) is an unusual type of cardiomyopathy characterized by a restriction to the ventricular filling and an obliteration of the inflow portion in the ventricular cavity by a fibrosis and often by a thrombus formation. The atrioventricular valve may be involved, resulting in an atrioventricular valvular regurgitation. The only known effective treatments are endomyocardiectomy and replacement of regurgitant AV valves. We report the experience of a case of EMF which required surgical management.
Cardiomyopathies
;
Endomyocardial Fibrosis*
;
Fibrosis
;
Thrombosis
9.Analysis of antibiotic residues in milk from healthy dairy cows treated with bovine mastitis ointment using ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry.
Eun Kee PARK ; Yong Jae RYU ; Chun Nam CHA ; Chang Yeul YOO ; Suk KIM ; Hu Jang LEE
Korean Journal of Veterinary Research 2016;56(4):233-239
This study was conducted to analyze penicillin G (PEG), streptomycin (STR) and neomycin (NEO) residues in milk of healthy lactating cows. Milk samples were collected from all four quarters of 12 dairy cows 2–7 days after intramammary infusions of an ointment containing PEG, STR and NEO once (n = 4; group I) or twice (n = 4, group II) daily. Ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry was used to determine the antibiotic residues in the samples. The correlation coefficient (r 2) of the calibration curves for all antibiotics was > 0.999 and the limits of detection and quantification were 0.002–0.005 µg/mL and 0.007–0.02 µg/mL, respectively. Recovery rates were ranged from 75.5 to 92.3%. In group I, PEG, STR and NEO residues were detected in milk at 2, 3 and 2 days post-treatment, respectively, which were below the maximum residue limit (MRL). In group II, PEG, STR and NEO residues were detected in milk at 2, 3 and 3 days post-treatment, respectively, which were bellow the MRL. These results suggest that a 3-day for milk withdrawal period after the ointment treatment might be sufficient for reduction of the antibiotic residues below the MRL.
10.A Single Center Analysis of the Positivity of Hepatitis B Antibody after Neonatal Vaccination Program in Korea.
Yong Joo KIM ; Peipei LI ; Jong Myeon HONG ; Keun Ho RYU ; Eunwoo NAM ; Mi Soo CHANG
Journal of Korean Medical Science 2017;32(5):810-816
The antibody to hepatitis B surface antigen (anti-HBs) seropositivity rate after 3 doses of hepatitis B virus (HBV) vaccination during infancy period is known to be higher than 90%. However, a considerable number of vaccines do not form protective anti-HBs or chronologic decrease of anti-HBs. We retrospectively collected data of HBV serologic test results in 20,738 individuals from 2000 to 2015. After exclusion criteria were applied, 19,072 individuals were included. We analyzed the anti-HBs seropositivity rate, anti-HBs disappearance rate, anti-HBs positive seroconversion rate after receiving a booster vaccine, and the difference in anti-HBs positivity between the 2 groups; group A (born before 2005, while both recombinant vaccines and plasma-derived vaccines were used) and group B (born after 2005, when only recombinant vaccines were used by national regulation). The anti-HBs seropositivity rate was 55.8%, but there was a significant difference in the rate of seropositivity for anti-HBs between the group A and B (53.0% vs. 78.1%, P < 0.001). There was no significant age-adjusted difference in the mean seropositivity rate between the 2 groups (P = 0.058). In addition, the anti-HBs positivity rate was significantly lower in the group A as compared with the group B during infancy (83.1% vs. 92.1%, P < 0.001). A total of 1,106 anti-HBs-positive subjects underwent serologic tests more than twice. Of these, 217 subjects (19.6%) showed anti-HBs disappearance. After booster vaccinations, 87.4% (83/95) achieved seroconversion from seronegative to seropositive. Our results highlight the importance of lifelong protection against HBV and the possible necessity of booster vaccination after adolescent period.
Adolescent
;
Child
;
Hepatitis B Surface Antigens
;
Hepatitis B virus
;
Hepatitis B*
;
Hepatitis*
;
Humans
;
Korea*
;
Retrospective Studies
;
Seroconversion
;
Serologic Tests
;
Vaccination*
;
Vaccines
;
Vaccines, Synthetic