Basal-like breast carcinoma is a phenotype of breast cancer which was sub-classified according to gene expression profiling criteria, associated with specific pathological features and biological behaviour. These cancer used to be more aggressive and characterized by the absent expression of ER/PR/CerbB-2(triple negative, TG) however positive for basal cytokeratins(basal CK). We present this review to show its pathological characters, clinical progression, prognosis and response to chemotherapy, and provide convenience for pathologists and surgical colleagues be more aware of this subtype.

Objective: To study the interaction between heat shock protein 90 (HSP90) and the survivin in human breast cancer cells and its effect on proliferation and apoptosis of tumor cells. Methods: The human breast cancer MDA-MB-231 cells were treated with an HSP90 inhibitor, geldanamycin (GA). The expression of phosphorylated signal transducers and activators of transcription 3 (P-STAT3) and survivin protein were detected by Western blotting. The mRNA transcription of sruvivin was determined by RT-PCR. The cell proliferation was measured by MTT assay. Apoptosis was examined by flow cytometry. Results: HSP 90 inhibitor GA reduced the expression of P-STAT3 and survivin, inhibited the proliferation, and induced apoptosis of human breast cancer MDA-MB-231 cells. Conclusion: HSP90 affected the transcription of survivin mRNA through activation of the JAK-STAT3 signaling pathway. Surviving-HSP90 interaction contributed to the promotion of the high-proliferation and low-apoptosis malignant behaviors of human breast cancer MDA-MB-231 cells.

Objective To examine the relationship among gratitude,coping styles,and pathological Internet use (PIU) in secondary vocational school students.Method 1 011 secondary vocational school students were tested with gratitude questionnaire,coping style questionnaire and pathological internet use questionnaire.Results The scores of gratitude,problem-solving,help-seeking,guilty,illusion,withdrawal,rationalization and PIU were respectively (5.23 ± 1.06),(0.65± 0.21),(0.50± 0.22),(0.40± 0.23),(0.47 ±0.22),(0.51 ±0.18),(0.42±0.18),(2.23±0.97).Gratitude was significantly and negatively associated with PIU(r=-0.18,P＜0.01).Moreover,the effect of gratitude on PIU was totally mediated by problem-solving,guilty,illusion,and rationalization (27.27％,33.43％,29.72％,9.58％).However,the mediating effects of help-seeking and withdrawal were not significant.Conclusion Coping styles mediate gratitude and PIU,and coping styles should be considered during pathological internet use of secondary vocational school students.

Objective:To explore the effective treatment to the depression after silent cerebral infarction(SCI).Methods:Seventy-three patients suffering from depression after SCI were treated for three moths with a mono-blind experiment.Results:There was significant difference between the effect of antidepressant and the effect of nonantideressant( P

Under stimulation of electric current adult albino mice were made to run around on the wire without stop for 1 to 4 hours in a wooden case. In starved animals glycogen began to decrease 60 minutes after depri- vation of food and reached to about half within 2 to 3 1/2 hours. Six hours later only a few liver cells still contained some glycogen granules. Deple- tion of ribonueleic acid took place at a much slower rate. It broke from larger masses into tiny rods and filaments, sparsely distributed. No change of nucleus and nucleolus was noted. In exhausted animals changes of liver glycogen and ribonucleic acid were much more conspicuous than the starved ones. In most cases 2 to 3 1/2 hours' fatigue caused complete disappearance of glycogen granules and the reduc- tion of the ribonucleic acid. The chromatin condensed into larger knots or adhered to the inner side of the nuclear membrane. Central veins and sinusoids became enlarged and congested. Liver cells became smaller. Kupffer cells increased in number and their nuclei became darker.

Adult albino rats, weighing 140-380 grams, were chosen for study on the effects of starvation, anaesthesia and nervous stimulation on liver glyco- gen and ribonucleic acid. Tissues were fixed in cold Carnoy's fluid, embedded in paraffin, stained with McManus' PAS method for glycogen and with methyl green-pyronine method for ribonucleic acid. Glycogen decreased considerably after 5 to 6 hours starvation and disap- peared completely after 8 hours. Ribonucleic acid also showed signs of decomposition and became more diffusely distributed. Anaesthetic starvation after intraperitoneal injection of 1% sodium amytal had less effect than that of starvation. When sciatic nerves of old rats were stimulated with 0.7-0.9 volts inducted current for 4 to 6 hours, liver glycogen disappeared completely and the depletion of liver ribonucleic acid was more pronounced than that of starvation. With young rats of 2 to 3 months of age, the influence was indistinct.

Objective: To study the effect of different dissolution media on the dissolution curve of risperidone oral soluble films to provide reference for the quality evaluation of the preparation.

ObjectiveTo undertake a controlled, prospective study to investigate the relationship between serum resistin level and blood sugar, blood lipids and thyroid hormone in patients with hyperthyroidism. Methods Clinical data of 50 patients with hyperthyriodism were collected from 2008 to 2009 in department of endocrinology the Second Hospital of Harbin Medical University. All patients were newly diagnosed cases that received no medication. At the same time, 40 healthy persons underwent physical examination in the hospital were taken as control group. Diabetes, obesity, hypertension, and high cholesterol were excluded from the two groups. Serum resistin level was determined using ELISA method. Chemiluminescence was used to check the levels of fasting insulin, free triiodothyronine (FT3), free thyroxin (FT4), and thyroid stimulating hormone (TSH). Glucose oxidase method determination-peroxidase-anti-peroxidase(GOD-PAP) was used to check the levels of fasting blood-glucose.Cholesterol oxidase method was used to check the levels of total cholesterol(T-CH). Glycerol-phosphate oxidase was used to check the levels of triglyceride(TG). Enzymes colorimetric method was used to check high density level of lipoprotein cholesterol(HDL-C) and low density level of lipoprotein cholesterol (LDL-C), and the height, weight,waist circumference, and hip circumference were measured. Body mass index (BMI) and insulin resistance index (HOMA-IR) were calculated. Groups were compared using t test and correlation analysis using Pearson correlation test, and the relationship between serum resistin level and blood sugar, blood lipids and thyroid hormone were analyzed using multiple linear stepwise regression analysis method. Results Serum glucose[(5.2 ± 0.7)mmol/L],resistin concentrations[(132.1 ± 41.3)μg/L], FT3[(19.8 ± 8.7) pmol/L], FT4[(54.1± 29.6)pmol/L], fasting insulin levels[(7.9 ± 2.8)mU/L] and HOMA-IR(2.3 ± 1.0) were significantly higher compared with controls group [(4.7 ± 0.5)mmol/L, (65.1 ± 5.9)μg/L, (4.1 ± 0.6)pmol/L, (14.3 ± 2.2)pmol/L, (6.4 ± 2.7)mU/L, (1.5 ±1.2); t =4.64, 10.17, 11.42, 8.49, 4.48, 9.42, P＜ 0.01 or ＜ 0.05)]. T-CH[(3.7 ± 0.8) mol/L], LDL-C[(1.8 ±0.6)mol/L], TSH[(0.01 ± 0.01 )mU/L] were significantly lower compared with controls group[(4.6 ± 0.7)mol/L,(2.3 ± 0.7)mol/L, (1.80 ± 0.90)mU/L; t =5.30, 3.33, 14.48; all P＜ 0.01)]. Pearson correlation analysis showed that resistin and FT3, FT4 and HOMA-IR was positively correlated, respectively (r =0.719, 0.790, 0.396, P ＜ 0.01or＜ 0.05), resistin and T-CH and LDL was negatively correlated, respectively(r =- 0.364, - 0.519, P ＜ 0.05or ＜ 0.01). Multiple linear regression analysisshowed that resistin and FT3, FT4, and HOMA-IR was positively correlated, respeetively(r =0.756, P ＜ 0.01 ). Conclusion Resistin and FT3 and FT4 are related. Resistin might play important roles in insulin resistance and glucose and lipid metabolism disorders in patients with hyperthyriodism.

Objective To study the influence of Salvia miltiorrhiza bge. f. alba(SMA) on apoptosis and Bcl-2 expression in human umbilical vein endothelial cell (HUVEC) in vitro. Methods HUVECs were isolated using perfusion and enzyme digestion methods, and the obtained cells were identified by morphological observation and VIII = Ag immunoreactivity examination. The cells in the exponential phase of growth were treated with H2O2 and different concentrations of SM A (high dose, 0.10 g/ml, low dose, 0.01 g/ml). The cell apoptosis was determined by flow cytometric analysis and Bcl-2 expression was examined by immunofluorescence mehtod. Results SMA significantly decreased the H2O2-induced apoptosis of HUVECs (P<0.01 in high dose group and P<0.05 in low dose group), and significantly increased the expression of Bcl-2 in high dose group(P<0.01). Conclusion SMA can inhibit H2O2-induced apoptosis of HUVEC, which might be associated with the increase of Bcl-2 expression. Salvia miltiorrhiza bge. f. alba; umbilical veins; endothelial cell; apoptosis; bcl-2 genes.

Humans ; Nephritis ; etiology ; therapy ; Prognosis ; Purpura, Schoenlein-Henoch ; complications ; therapy ; Sjogren's Syndrome ; etiology ; therapy