1.Serum Eosinophil Cationic Protein Levels in Patients with Allergic Diseases.
Young Joo CHA ; Seok Lae CHAE ; Eun Ah CHANG
Korean Journal of Clinical Pathology 1999;19(3):348-352
BACKGROUND: Eosinophil cationic protein (ECP), one of the eosinophil granule proteins released during allergic reactions, may play a major role in the allergic inflammatory process. The measurement of ECP in serum may be a useful indicator of eosinophil activity in ongoing inflammatory processes. We investigated the clinical utility of ECP measurement in serum in patients with bronchial asthma, allergic rhinitis and atopic dermatitis, after standardizing sample processing. METHODS: We measured the serum ECP levels in patients with bronchial asthma (n=38), chronic obstructive pulmonary diseases (COPD) (n=13), respiratory symptoms (n=19), allergic rhinitis (n=26), non-allergic rhinitis (n=24), and atopic dermatitis (n=10) and in normal healthy controls (n=16) by the fluoroenzyme immunoassay using Pharmacia CAP System, and evaluated the correlation between ECP level and blood eosinophil number, or ECP and IgE levels. Blood eosinophil number was counted by the automated cell counter. RESULTS: Serum ECP levels were significantly higher in patients with bronchial asthma (15.6+/- 12.6 g/L), COPD (13.3+/-7.2 g/L), allergic rhinitis (23.8+/-13.2 g/L), and atopic dermatitis (20.6+/- 18.4 g/L) than in normal controls (7.5+/-4.2 g/L) (P <0.05). ECP levels were also significantly higher in patients with bronchial asthma and COPD than in patients with simple respiratory symptoms (6.9+/-4.7 g/L), whose ECP levels did not statistically differ from those in normal controls. ECP levels were also significantly higher in patients with allergic rhinitis than in patients with non-allergic rhinitis (9.5+/-5.1 g/L), whose ECP levels did not statistically differ from those in normal controls. Serum ECP level and eosinophil number in peripheral blood were correlated only in patients with bronchial asthma (r=0.53, P <0.01) and no correlation between ECP and IgE levels was found in all of the patients. CONCLUSIONS: ECP is the one of the secretory components released from the eosinophil granule and measurement of ECP in serum might be one of the noninvasive tool to assess the activity in relation to eosinophil involvement in various allergic diseases.
Asthma
;
Cell Count
;
Dermatitis, Atopic
;
Eosinophil Cationic Protein*
;
Eosinophil Granule Proteins
;
Eosinophils
;
Humans
;
Hypersensitivity
;
Immunoassay
;
Immunoglobulin E
;
Lung Diseases, Obstructive
;
Pulmonary Disease, Chronic Obstructive
;
Rhinitis
2.An Experimental Study of Osteoporosis Produced by Oophorectomy and /or Immobilization in Rats
Seok Hyun LEE ; Eung Nam CHA ; Chang Sung CHO
The Journal of the Korean Orthopaedic Association 1995;30(3):779-786
Previous studies implied association of osteoporosis with estrogen deficiency, immobilization and low calcium absorption only through epidemiological studies. There have been only a few experimental studies verifying the etiologic factors of osteoporosis in vivo condition. Authors conducted an experimental study using white rats(Sprague Dawley) in order to find out what the endocrinological and biochemical changes of experimentally induced osteoporosis are and how they behave with or against each other. White rats, eighty five in number of 3 to 6 months females and weighing 220±12.7gm were divided into four groups. They consisted of Group I(n=10) for control, Group II(n=25) for bilateral oophorectomy, Group III(n=25) for bilateral division of sciatic nerve and hip spica cast immobilization, and Group IV(n=25) for bilateral oophorectomy and bilateral division of sciatic nerve plus hip spica cast immobilization. Blood samples were taken preoperatively and postoperatively at six weeks to check Estradiol and Osteocalcin levels there of. And then, rats were sacrified immediately after the second sampling to retrieve femora for bone mineral density measurement and torsional stress test. Estradiol levels before operation were 21.4±13.3pg/ml for Group I, 31.6±3.1pg/ml for Group II, 25.6±4.5pg/ml for Group III and 33.7±4.5pg/ml for Group IV, respectively. There were no significant differences observable among the groups. Estradiol levels at six weeks postoperatively were 42.3±18.8pg/ml for Group I, 5.4±2.7pg/ml for Group II, 40.8±5pg/ml for Group III and 6.2±2.3 pg/ml for Group IV, respectively. Apparent reductions in group II and IV were proved of statistical significance. Osteocalcin levels preoperatively were 1.2±0.6ng/ml for Group I, 1.7±0.4ng/ml for Group II, 1.5±0.1lng/ml for Group III and 1.5±0.1.lng/ml for Group IV, respectively. At six weeks postoperatively they were 1.6±0.1lng/ml for Group I, 1.7±0.3ng/ml for Group II, 1.8±0.3ng/ml for Group III and 1.2±0.1lng/ml for Group IV, respectively. The differences and changes among the groups and measurements were not of statistical significance. Bone mineral contents at six weeks postoperatively were 0.248±0.03g for Group I, 0.177±0.03g for Group II, 0.226±0.04g for Group III and 0.092±0.01g in Group IV, respectively. Low values of Group II and IV compared to those of Group I and III were of statistical significance.(P=0.0001) Torsional strength of bones at six weeks postoperatively were 4.0±0.2N/m for Group I, 1.5±0.1N/m for Group II, 1.5±0.1N/m for Group III and 1.4±0.1N/m for Group IV, respectively. Decreases of experimental groups(II, III, IV) compared to that of control group(I) were of statistical significance, but differences among the experimental groups were not of significance(p>0.05). For above observations, it was possible to conclude that osteoporosis measurable by bone mineral content and torsional stress test was caused by oophorectomy and immobilization in vivo, the former of which was more rapid and profound than the latter during early phase. When both factors, i.e., oophorectomy and immobilization are exerated simultaneously. the resultant osteoporosis was found in higher degree than either factor only, but not at incremental degree as one may expect.
Absorption
;
Animals
;
Bone Density
;
Calcium
;
Epidemiologic Studies
;
Estradiol
;
Estrogens
;
Exercise Test
;
Female
;
Hip
;
Humans
;
Immobilization
;
Osteocalcin
;
Osteoporosis
;
Ovariectomy
;
Rats
;
Sciatic Nerve
3.The Clinical Experience of Leiomyoma of the Female Urethra.
Chang Seok CHA ; Jeong Zoo LEE
Korean Journal of Urology 2001;42(3):347-349
PURPOSE: Leiomyoma of the urethra is a rare disease entity. We treated leiomyoma of the female urethra surgically during the period from 1981 to 1997 and retrospectively reviewed the results. MATERIALS AND METHODS: We have experienced 11 cases of the leiomyoma of the female urethra. History taking, clinical symptoms, urinalysis, laboratory findings, cystoscopic finding, postoperative complication and follow-up examination were observed retrospectively. Also, we checked pelvic computed tomography in 2 cases which had been detected uterine mass at pelvic ultrasonography. RESULTS: Average age of the patients was 40 years (28-51) and the chief complaint was a painless mass of anterior vaginal wall. All masses were removed surgically and confirmed as leiomyoma histologically. The uterine leiomyoma was combined in 2 cases. There were no recurrence or complication after surgery until now. CONCLUSIONS: A surgical removal of the leiomyoma of the female urethra provided a long term disease free survival and the search for the associated pelvic leiomyoma should be done.
Disease-Free Survival
;
Female*
;
Follow-Up Studies
;
Humans
;
Leiomyoma*
;
Postoperative Complications
;
Rare Diseases
;
Recurrence
;
Retrospective Studies
;
Ultrasonography
;
Urethra*
;
Urinalysis
4.Determination of Reduced Glutathione in Red Blood Cells.
Eun Ah CHANG ; Sung Won KIM ; Seok Lae CHAE ; Young Joo CHA
Korean Journal of Clinical Pathology 1997;17(2):238-244
BACKGROUND: The product of oxygen-free radicals inf1ict oxidative injuries on healthy cells. Antioxidants such as superoxide dismutase(SOD), glutathione peroxidase, and reduced glutathione(GSH) are present in almost all cells and play important roles in metabolism, transport, and cellular protection. We measured blood GSH levels in healthy controls and patients with non insulin dependent diabetes mellitus(NIDDM) for evaluation of the clinical usefulness of GSH. METHODS: Erythrocyte GSH levels were measured in fifty healthy controls and thirty NIDDM patients with diabetic retinopathies by Beutler's method. We also tested within-run precision, between-run precision, linearity and recovery rate to evaluate this method measuring erythrocyte GSH levels. RESULTS: The GSH levels (mean +/-SD) of NIDDM patients (5.03+/-0.67mumo1/Hb) were significantly lower than those of healthy control group (6.46+/-0.85mumo1/Hb)(P<0.001). The results of within-run precision and between-run precision when stored at 4degrees Cwere excellent (coefficient of variation were 2.79% and 2.42%, respectively), however, when stored at the room temperature the GSH levels were sharply declined. The linearity and recovery rate were acceptable. CONCLUSIONS: The prescision, linearity, and recovery rate of GSH measurement were excellent. The GSH levels in NIDDM patient group were reduced, and this probably contributes to the defective defense mechanism against increased oxidative stress. Additional measurement of other antioxidants such as superoxide dismutase and glutathione Peroxidase may be required to clarify the pathologic significance of glutathione metabolism in various diseases.
Antioxidants
;
Diabetes Mellitus, Type 2
;
Diabetic Retinopathy
;
Erythrocytes*
;
Glutathione Peroxidase
;
Glutathione*
;
Humans
;
Insulin
;
Metabolism
;
Oxidative Stress
;
Superoxide Dismutase
;
Superoxides
5.A case of monostotic paget's disease
Cheol Min PARK ; Jung Hyuk KIM ; In Ho CHA ; Chang Hong LEE ; Seok Hyun LEE ; Kap No LEE
Journal of the Korean Radiological Society 1985;21(2):323-327
Paget's disease of bone (osteitis deformans), a chronic osseous disease of unknown etiology, is extremely rarein Asia although well known in western countries. This disease is characterized by the simultaneous absorption ofbone and the new bone formation of abnormal character affecting middle and later age groups. The authorsexperienced a case of monostotic Paget's disease involving left pelvic bone in 56-years old female. To ourknowledge, this is the first cas report of monostotic Paget's in Korea.
Absorption
;
Asia
;
Female
;
Humans
;
Korea
;
Osteitis Deformans
;
Osteogenesis
;
Pelvic Bones
7.Factors Affecting the Improvement of Olfactory Disturbance in Patients Undergoing Endoscopic Sinus Surgery for Chronic Sinusitis.
Korean Journal of Otolaryngology - Head and Neck Surgery 2005;48(2):178-181
BACKGROUND AND OBJECTIVES: The most common cause of olfactory dysfunction is chronic rhinosinusitis, and endoscopic sinus surgery has been reported to yield beneficial outcome for the olfactory function. The purpose of this study was to study the benefits of endoscopic sinus surgery and investigate the influence of prognostic variables on the olfactory function in chronic rhinosinusitis patients. MATERIALS AND METHOD: We enrolled 52 patients who underwent endoscopic sinus surgery for chronic rhinosinusitis with olfactory dysfunction from July 2000 through April 2003 at Bundang CHA General Hospital, College of Medicine, Pochon CHA University. Patients were classified into 3 groups (well-healing, persistent olfactory disturbance and worse groups). We statistically compared various prognostic variables, such as CT scores, age, gender, allergy, duration of disease. and previous nasal surgery history among the 3 groups. KVSS test was used (Korean Version of Sniffin' Sticks test) for the evaluation of olfactory function. RESULTS: Of the prognostic variables examined, CT scores, gender, allergy, and duration of disease had no influence on the prognosis of olfactory improvement. However, old age and previous nasal surgery were found to have statistically significance on the worse prognosis. The olfactory function improved in 88% of the patients following the endoscopic sinus surgery. CONCLUSION: We concluded that olfactory function of many patients with conductive disorder improved after endoscopic sinus surgery. Gender, allergy, duration of disease had no singnificant influence on the prognosis of olfaction. The severity of sinusitis and the extent of polyps were not consistent with the degree of olfactory repairment
Hospitals, General
;
Humans
;
Hypersensitivity
;
Nasal Surgical Procedures
;
Polyps
;
Prognosis
;
Sinusitis*
;
Smell
8.Gadolinium enhanced MRI findings of bell's palsy and herpes zoster oticus.
Chang Il CHA ; Sang Ryeol SEOK ; Dong Yeup LEE ; Joong Saeng CHO ; Hwoe Young AHN
Korean Journal of Otolaryngology - Head and Neck Surgery 1993;36(3):498-505
No abstract available.
Bell Palsy*
;
Gadolinium*
;
Herpes Zoster Oticus*
;
Herpes Zoster*
;
Magnetic Resonance Imaging*
9.A case of secondary amyloidosis in the larynx.
Hwoe Young AHN ; Sang Ryeol SEOK ; Pyeong Gwi JUNG ; Chang Il CHA
Korean Journal of Otolaryngology - Head and Neck Surgery 1992;35(3):457-461
No abstract available.
Amyloidosis*
;
Larynx*
10.Cervical bronchogenic cysts mimic metastatic lymph nodes during thyroid cancer surgery.
Hak Hoon JUN ; Seok Mo KIM ; Yong Sang LEE ; Soon Won HONG ; Hang Seok CHANG ; Cheong Soo PARK
Annals of Surgical Treatment and Research 2014;86(5):227-231
PURPOSE: Although congenital bronchogenic cysts in the cervical region, especially in the thyroid or perithyroidal area, are rare, distinguishing them from other cervical cystic lesions (e.g., thyroglossal duct and branchial cleft cysts) and metastatic cervical lymph nodes is difficult preoperatively. Additionally, cystic degeneration of metastatic lymph nodes is common in patients with thyroid cancer. We investigated the clinical characteristics and proper treatment for individuals with cervical bronchogenic cysts. METHODS: Of the 18,900 patients treated for thyroid cancer, 18 patients with pathologically confirmed bronchogenic cysts were retrospectively reviewed. Bilateral total thyroidectomy or less than total thyroidectomy with central compartment node dissection, including cystic mass excision was done and cystic mass was confirmed by postoperative pathologic examination. RESULTS: All cervical bronchogenic cysts were asymptomatic. Their mean size was 1.2 cm (range, 0.3 to 3 cm). Of these 18 patients, 15 did not have any abnormal radiological findings, except for lymphadenopathy during preoperative evaluations. Most bronchogenic cysts were detected around the thyroid and paratracheal areas. On preoperative imaging and intraoperatively, most were indistinguishable from metastatic cervical lymph nodes or other cystic lesions. CONCLUSION: Although cervical bronchogenic cysts are rare and benign, they should be distinguished from other cystic cervical masses, especially metastatic cervical lymph nodes associated with thyroid cancer. Possible cervical bronchogenic cysts found during thyroid cancer evaluation or surgery should be surgically excised.
Branchial Region
;
Bronchogenic Cyst*
;
Humans
;
Lymph Nodes*
;
Lymphatic Diseases
;
Retrospective Studies
;
Thyroid Gland
;
Thyroid Neoplasms*
;
Thyroidectomy