AIM: To observe effects of homocysteine and antagonized effects of taurine on electronic leakage and free radical production in myocardial mitochondria. METHODS: Myocardial mitochondria of rat heart was isolated, and was broken by supersonic wave to prepare submitochondria. Recombinant of succinic acid cytochrome c reductase was prepared with mitochondria of porcine heart. They were co-incubated with homocysteine and/or taurine with various concentration. The H_2O_2 and O_2~ were determined by chemiluminescence methods. The taurine transporter of heart mitochondria and its propert, and effects of homocysteine on its function were studied with glass filter. RESULTS: Homocysteine stimulated oxygen free radical production in heart mitochondria, submitochondria, and succinic acid cytochrome c in a concentration-dependent manner. Although taurine itself did not affect oxygen free radical production, taurine did inhibit oxygen free radical production in mitochondria, submitochondria and succinic acid cytochrome c in a concentration-dependent manner. Taurine transporters of Na~+-dependent were existed in mitochondria membrane. Homocysteine inhibited taurine transtport in mitochondria in a concentration-dependent manner. CONCLUSIONS: Taurine inhibited electronic leakage and oxygen free radical production induced by homocysteine in electron transport chain. There were taurine transporters in mitochondria membrane, and transport functions of taurine transporter were inhibited by homocysteine. [

Objective To investigate the relationship between levels of homocysteine ( Hcy ) and serum uric acid( UA) and stability of carotid atherosclerotic plaque in young patients with cerebral infarction .Methods 150 cases of cerebral infarction in young patients carried the carotid ultrasonography were divided into the non -plaque group ( n=37),stable plaque group(n=52) and unstable plaque group(n=61) according to the results.The levels of Hcy and UA in the three groups of patients were detected and analyzed statistically .Results The stable plaque group′s Hcy and UA levels were (15.92 ±2.52)μmol/L and (294.85 ±25.52)μmol/L,which were significantly higher than those of the non-plaque group(t=7.33,6.89,all P<0.05);The unstable plaque group′s Hcy and UA levels were (23.17 ±3.82 )μmol/L and ( 388.57 ±26.61 )μmol/L, which were significantly higher than those of the the non-plaque group and stable plaque group(t=9.82,10.02,6.90,7.12,all P<0.05).Conclusion There is a close correlation between the stability of carotid artery plaque and the levels of Hcy and UA .

Objective To explore the clinical significance of joint detection of β2‐microglobulin(β2‐MG) ,glycated hemoglobin and Cystatin‐C (CysC) in early renal injury in patients with diabetes mellitus .Methods 60 patients with early diabetic renal damage were in early diabetic renal damage group and 100 patients with simple diabetes mellitus were in simple diabetes mellitus group .50 healthy persons were the control group .Using immunoturbidimetry to detect the serum level of CysC ,immunoturbidimetry trans‐mission to detect the urine level of β2‐microglobulin and ion exchange high performance liquid chromatography method to detect the level of HbA1c .Results There were statistically significant differences in the level of β2‐MG ,glycated hemoglobin between early diabetic renal damage group and the other two groups (P<0 .05) .There were statistically significant differenees in the level of gly‐cated hemoglobin between simple diabetes mellitus group and healthy control group (P<0 .05) .Conclusion It is important for pa‐tients with diabetes mellitus to detect the β2‐MG ,glycated Hemo globin and CysC in diagnosis ,monitoring .prevention of early renal injury .

Objective To explore the short-term effect of oral antibiotic at different times on the treatment of invasive periodontitis. Methods 78 patients with invasive periodontitis from November 2015 to March 2017 were enrolled in this study. Randomized method was used to divide the patients into the observation group and the control group, 39 cases in each group. On the basis of the basic treatment, the observation group were received amoxicillin and metronidazole after oral prophylaxis, the control group were received amoxicillin and metronidazole after subgingival scaling. The plaque status before and after the basic treatment and the efficacy in the two groups after 8 weeks were compared. Results The plaque in the two groups was significantly improved after basic treatment (P<0.05). There was no significant difference in the probing depth and the loss of attachment between the two groups. The bleeding index in the observation group was significantly lower than that in the control group (P <0.05). The probing depth in the observation group was significantly better than that in the control group (P<0.05). Conclusion On the basis of basic therapy, taking antibiotics has a better therapeutic effect on the patients with invasive periodontitis. Antibiotics is use after oral prophylaxis is better than it used after subgingival scaling, which is worthy to be popularized in clinical practice.

Objective To investigate the factors that influence the occurrence of hemorrhagic transformation (HT) in patients with acute massive cerebral infarction,and to provide references for clinical treatment.Methods The clinical data of 60 patients with acute ischemic stroke were retrospectively analyzed.All the 60 patients were divided into HT group and non-hemorrhagic transformation (NHT) group.Risk factors associated with cerebrovascular disease,such as general data (gender,age,histories of drink and smoke),history of past illness (hypertension,diabetes,coronary disease,atrial fibrillation and cerebral stroke) and clinical data (infarction location,therapeutic methods,blood pressure,blood glucose,blood fat,cholesterol,fibrinogen and et al),were compared between the two groups.Results Of all the 60 patients,HT rate was 35％ (21/60).Incidence of HT was higher in patients with past and present hypertension and hyperglycemia(P =0.009,0.013,0.005,0.008,0.017),and in patients with atrial fibrillation,anti-platelet therapy,infarction in cortex,and hypocholesterolemia (P =0.020,0.037,0.003,0.009).But gender,age,histories of drink and smoke and history of coronary disease were not significantly different between the two groups(P=0.935,0.309,0.196,0.717,0.058).Conclusion Hypertension,hyperglycemia,atrial fibrillation,hypocholesterolemia,anti-platelet therapy could be independent risk factors for HT in patients with acute massive cerebral infarction.

Objective To compare and investigate the criteria of impairment evaluation about eye injury.MethodsThe distill items, contents of these items and excursus of three criteria, Assessment and Gradation of Disability Caused by Work-related Injuries and Occupational Diseases (AGDCWIOD), Assessment for Body Impairment of the Injured in Road Traffic Accident (ABIIRTA) and Assessment and Gradation of Impairment Caused by Trauma (AGICT), commonly used in justice practice at present were compared.ResultsThere were essential differences among three criteria. AGDCWIOD was loose, ABIIRTA and AGICT were strict. AGDCWIOD and AGICT were religious, ABOIIRTA was oversight and non-religious. At the same time, there were differences between domestic and international criteria, and domestic criteria were behind the times.ConclusionThere are many problems in the criteria of impairment evaluation about eye injury; related items need recension and consummation.

Some factors may induce accidents during physiotherapy for stroke patients. This article would summarize the general risk factors and discuss the management.

ObjectiveTo assess a simple apparatus measuring displacement of pelvis in clinical application.MethodsTwo testers measured the displacement of pelvis of 20 healthy young subjects moving their body forward, backward, leftward and rightward in standing position. The reliability of inter- and intra-tester was tested with intraclass correlation co-efficiency (ICC). And the validity between this method and Functional Reach Test (FRT) was tested.ResultsThe ICC of inter- and intra-tester were 0.91～0.97 and 0.94～0.98 respectively, and correlated with FRT (r=0.88, P<0.01).ConclusionThis simple apparatus can be used in a balance test.

Humans ; Medicine, Chinese Traditional ; Sleep Wake Disorders ; classification ; etiology ; therapy