Objective To study the effect of PPARγ ligand troglitazone (TGZ) on invasion and metastasis of gastric cancer cells, and investigate the relationship of PPARγ ligand with gastric cancer.Methods The expression of PPARγ in gastric cancer cell line MGC803 were detected by immunofluorescence cytochemistry method. The effect of different density TGZ on proliferation activity and adhesion of gastric cancer cell were detected by MTT chromatometry. The effect of different ligands on invasion and metastasis of gastric cancer cell MGC803 were detected by invasion system in vitro. Results The expression of PPARγ mainly located in cell nucleus. TGZ inhibited the proliferation of gastric cancer cell, decreased cell adhesion, locomotory capacity and invasion to matrigel, which had time and dose-dependent relationship.When treatment with 0. 1,1.0 and 10μ mol/ L TGZ, inhibition ratio of invasion and metastasis of cell was 8.79% ,31.31% ,51.42% and 28.29% ,4. 27% ,59. 27% respectively, which had statistical significance compared with control group( P <0. 05). When treatment was 10μ mol/L TGZ, cell adhesion was 0. 32 ±0. 03, it was statistically significant higher than that in control group (0. 52 ± 0. 04, P < 0. 05 ). Conclusion Human gastric cancer cell line MGC803 expressed functional PPARγ protein. TGZ inhibited adhesion and invasion of MGC803 cell on ECM at different degree, the effect of combination of two ligands was evident, which mechanism of action needed to be further investigated.

Cadmium ; adverse effects ; Dose-Response Relationship, Drug ; Environmental Exposure ; adverse effects ; Humans ; Occupational Exposure ; adverse effects ; Risk Assessment

Cadmium ; pharmacology ; Cells, Cultured ; Heat-Shock Proteins ; biosynthesis ; Humans ; Metallothionein ; biosynthesis ; Ubiquitin ; biosynthesis

Hand Disinfection ; Health Personnel ; Humans ; Infant, Newborn ; Intensive Care Units, Neonatal

Objective To examine the incidence of metabolic syndrome (MS) in incipient female patients with rheumatoid arthritis (RA), and study the relationship between MS and disease activity of RA. Methods The clinical data of 102 incipient female RA patients (RA group) and 125 healthy controls (control group) were retrospectively analyzed. The incidence of MS was compared between 2 groups, and the clinical and laboratory data were analyzed between patients with MS and patients without MS. Results There were no statistical differences in age, body mess index (BMI), waist circumference, systolic blood pressure, diastolic blood pressure, fasting blood glucose (FBG), triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C) between 2 groups (P>0.05). The incidences of waist circumference ≥ 80 cm, TG ≥ 1.70 mmol/L, HDL-C ≤ 1.30 mmol/L, MS according to the National Cholesterol Education Program Adult Treatment Panel Ⅲ (NCEP-ATPⅢ) standard and MS according to the Chinese Diabetes Society (CDS) standard in RA group were significantly higher than those in control group: 39.2% (40/102) vs. 25.6% (32/125), 37.3% (38/102) vs. 24.0%(30/125), 34.3%(35/102) vs. 21.6%(27/125), 34.3%(35/102) vs. 22.4%(28/125) and 27.5%(28/102) vs. 16.0% (20/125)%, and there were statistical differences (P<0.05). There were no statistical differences in the incidences of BMI≥25 kg/m2, systolic blood pressure ≥130 mmHg (1 mmHg = 0.133 kPa), diastolic blood pressure ≥85 mmHg and FBG≥5.6 mmol/L between 2 groups (P>0.05). The RA patients were divided into RA with MS (35 cases) and RA without MS (67 cases) according to the MS diagnostic standard of NCEP-ATPⅢ. The erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and blood uric acid in RA with MS patients were significantly higher than those in RA without MS patients:(68.1 ± 26.3) mm/h vs. (40.1 ± 30.6) mm/h, 4.260 mg/L vs. 1.505 mg/L and (330.5 ± 69.1)μmol/L vs. (269.0 ± 67.5)μmol/L, and there were statistical differences (P<0.05). There were no statistical differences in age, involved joint number, rheumatoid factor (RF), hemoglobin and incidence of anti-cyclic citrullinated peptide antibody (CCP antibody) between RA with MS patients and RA without MS patients (P>0.05). Conclusions The incidence of MS in incipient female patients with RA is higher than that in healthy controls, and RA patients are more prone to abdominal obesity, TG increasing and HDL-C decreasing. The inflammatory index and blood uric acid levels in RA with MS patients are significantly higher than those in RA without MS patients.

Objective The enhanced patterns of atherosclerotic plaque on dynamic contrastenhanced MRI have not been well studied. The aim of this study was to explore the patterns of plaque enhancement and their underlying mechanism by using dynamic contrast-enhanced MRI (DCE-MRI).Methods Atherosclerotic plaques were induced in the aorta of 12 New Zealand White rabbits by a combination of endothelial denudation and high-cholesterol diet. Ten to sixteen weeks after surgery, DCEMRI was performed with a fast spin echo T1 weighted sequence. Thirty-five phases of images were obtained at 71-second intervals. Gd-DTPA was injected coincident with the third scan via marginal ear vein. Specimens were harvested within 12 hours after imaging for HE staining and CD31 immunohistochemical staining which was used to highlight nco-vessels. Plaque enhancement patterns were studied and compared with histological findings. Signal intensity of each plaque section was normalized to pre-contrast signal intensity of psoas muscle, after which signal intensity versus time curve was drawn. Pearson correlation coefficient was used to reveal association between histological neo-vessel count and descriptive parameters derived from signal intensity versus time curve. Results Plaques were significantly enhanced by Gd-DTPA. Enhancement patterns could be described as fast-in and slow-out. Differences in patterns of enhancement were observed between tissues, with fibrous tissue enhanced more than lipid aggregation and leukocyte foci. Peak enhancement( 1. 05 ±0. 30) , initial slope(0. 82 ± 0. 28 ) and area under the curve at early phase(4.97 ± 1.67) derived from signal intensity-time curve had significant correlations with neo-vessel count( 117.7 ± 93. 3) ( r= 0. 553,0. 468, 0. 554 respectively, P ＜ 0. 05 ) . Conclusions The enhanced patterns of atherosclerotic plaque by Gd-DTPA were fast- in and slow-out. Neovascularization, increased endothelial permeability and extracellular matrix may be the reasons for plaque enhancement by Gd-DTPA. DCE-MRI has the potential to quantify the extent of neo-vasculature formation within plaques.

Objective To evaluate the effects of allografi and rhBMP-2 in posterolateral lumbar spinal fusion in a rabbit model.Methods Thirty rabbits were randomly divided into three groups:autogenous lilac crest bone graft group,rhBMP-2/allograft composite group,and allograft group.The animals were killed and sampled six weeks after the surgery.The lumbar intertransverse process fusion for the animals was assessed by manual palpation,biomechanical testing,radiography,histology and quantitative histology of spine fusion mass in a 6-week observation.Results The ratio of fusion in rhBMP-2/allograft composite group(90%)was significantly higher than that in autogenous lilac crest bone graft group(40%)and allograft group(20%)(P＜0.05).The autogenous lilac crest bone graft group and rhBMP-2/allograft composite group showed significantly higher uniaxial tensile strength than allograft group.The au- togenous lilac crest bone graft group and rhBMP-2/allograft composite group also showed significantly more new bone formation than allograft group,but there was no significant difference between the former two grnups.Conclusion rhBMP-2/allograft composite may be an ideal substitute for autograft in lumbar spinal fusion.

Background and purpose:Dendritic cells(DCs) possess specialized feature such as pathogen recognition,antigen capturing and processing machinery,and stimulating naive T lymphocyte to have antitumor ability that allow them to act like professional APCs.This paper is aimed to confirm the impacts on the proliferation and secretion of INF-? of tumor specific CTL and its cytotoxocity induced by DC loaded with different antigen.Methods:After the stimulation of DC loaded with different antigen,the proliferative rate of allolymphocytes was measured by MTT and the cytotoxocity of CTL was evaluated with LDH method.The INF-? secreted by activated T lymphocytes was detected by ELISPOT.Results:The DC loaded with CPP-Id(320%?15%) had significantly induce T lymphocyte proliferating when comparing with the induction by DC loaded with Id(57%?10%)(P

Objective:Based on relevant database data analysis,to explore the correlation of the abnormal expression of EMT related factors and chronic obstructive pulmonary disease and its related non-small cell lung cancer.Methods: Based on some data sets of GEO in the NCBI database,to perform express analysis,survival analysis and correlation analysis.Results: ①Snai1 and other EMT related regulatory factors exist significantly higher expression in non-small cell lung cancer patients,and E-cadherin (CDH1) was showed significantly lower expression. ②A large number of COPD patients samples were analyzed,and some EMT-related molecules in patients with COPD also showed significant abnormal expression and consistented with the changes in patients with non-small cell lung cancer.Conclusion: The results showed that dysregulation expression of EMT related regulatory factors may have some correlation with disease progression of COPD patients through the EMT markers and their expression and correlation analysis in COPD patients.