Objective To study for the relationship between the value of Triglyceride ( TG)/High Density Lipoprotein Cholesterol (HDL-C) Ratio and Coronary CT angiography .Methods Randomly se-lected elderly people between 65 to 80 years old with medical examination in our hospital in January 2008 -January 2010 , not taking lipid lowering drugs nearly half of the year , usually with activity or after resting precordial pain made of a total of 205.They divided into mild and severe group using the Siemens Somatom Definition dual-source 64-slice spiral CT of the selected candidates for coronary CTA , and lipid levels , TG/HDL-C ratio were been done at the same time .Results The level of TC/LDL-C and TG/HDL-C in Se-vere stenosis group was significantly higher than that in mild stenosis group ( P <0.01 , P <0.05 separate-ly) .While the level of HDL-C in Severe stenosis group was significantly lower than that in mild stenosis group ( P <0.05 ) .Logistic regression analysis showed the high TG /HDL ratio in severe coronary artery stenosis is an independent risk factor .Conclusion High TG /HDL-C ratio is relationship with moderate to severe coronary artery stenosis and which is independent risk factors to predict degree of severe coronary ar -tery stenosis.

Objective:To study the survival and the cardiac differentiation of mesenchymal stem cells from human fetal livers(FMSCs) after being transplanted into infarted rat myocardium. Methods:FMSCs were cultured from human fetal livers, the average age of which was 9 days. The infarction model was built by the ligation of left anterior descending artery and 3.5?106cells were transplanted into infarction area by direct injection one week after ligation. Rats were killed at 1 and 2 weeks after transplantation, and hearts were collected for the examination of the cells' survival and differentiation toward cardiac tissue by fluorescent in situ hybridization(FISH)and immunohistochemistry staining. Results:The FACS results showed that FMSCs expressed antigen CD29,CD44,CD166, SH2(CD105),SH3 and SH4, but did not express CD14,CD34 and CD45.The transplanted cells survived at 7th day after transplanted,but dissappeared at 14th day. No cardiac differentiation was observed during this period. Conclusion:The cells we cultured are testified as FMSCs by their phenotypic expression,and FMSCs could survive for a short period after transplanted into rat heart infarction model,but no cardiac differentiation is induced.

Objective: To explore the mechanism about the cardiac differentiation of human mesenchymal stem cells(MSCs) induced by rat cardiomyocytes.Methods:To induce the cardiac differentiation of human MSCs by using rat cardiomyocytes in different ways(three dishes for each way):(1) Cocultured with rat cardiomyocytes at the ratio of 1:1;(2)Cultured with the conditional medium,which got from the culture of rat cardiomyocytes and mixed with fresh medium by 1:1;(3) Cultured with the supernatant,which got from the minced rat cardiomyocytes and mixed with fresh medium by 1:10.The human MSCs were harvested at the day of 2,5 and 7,respectively.Then the cells were stained with desmin and cardiac troponin I monoclonal-antibodies,and fluorescent in situ hybridization to human DNA was used to mark the cells from human.Results: No desmin or cTnI was detected in the human MSCs cultured with conditional medium or supernatant.While for the human MSCs cocultured with rat cardiomyctes,desmin was detectable at the day of 2 and cTnI at the day of 5.Until the day of 7,about 35% of cocultured-human MSCs expressed those cardiac specific antigens.Conclusion:Human MSCs could be induced to differentiate into cardiomyocyte-like cells by coculture with rat cardiomyocytes,but not by the conditional medium or the supernatant of rat cardiomyocytes.

BACKGROUND:Aortic root homograft has been widely used, but it stil has a high incidence of calcification and degeneration after operation.
OBJECTIVE:To investigate the alteration of biological and immunological properties of rabbit aortic root homograft treated with de-endothelialization and decellularization methods, and to find a better decellularization method.
METHODS:Aortic root homografts obtained from rabbits were randomly divided into fresh group, de-endothelialization group, and decellularization group. The morphology of homografts was evaluated by hematoxylin-eosin, Masson and VG stainings. The biological properties such as thickness, tensile strength, broken extension rate, and heat shrinkage temperature were detected. The immunological features (inflammatory cellinfiltration, calcium content and CD152 expression) were determined with immunohistochemistry and flame atomic absorption spectrometry.
RESULTS AND CONCLUSION:As demonstrated in morphological observation, protocols of de-endothelialization and decellularization methods we used attained our desired aim of decellularization perfectly. Tensile strength and broken extension rate of decellularization group were decreased significantly compared with fresh group (P<0.01), while the difference between de-endothelialization group and fresh group was not significant (P>0.05). Hematoxylin-eosin staining showed that, inflammatory reaction was the most apparent in fresh group, showing a large amount of inflammatory cells infiltration;compared with fresh group, inflammatory reaction was significantly attenuated in de-endothelialization group, the number of inflammatory cells was significantly decreased. The inflammatory reaction was the lowest in the decellularization group, only inflammatory cells infiltrated the tissue edge. Immunohistochemistry showed that, CD152 expression was significantly increased in de-endothelialization and decellularization groups compared with fresh group (P<0.05). Calcium content in de-endothelialization and decellularization groups was significantly decreased compared with fresh group (P<0.05). It could be concluded that the immunogenicity of de-endothelialized and decellularized aortic homografts is decreased significantly, and the de-endothelialization method could better maintain the tensile property.

Pulmonary cavitary lesions in children consist of a group of heterogeneous diseases, mainly caused by infections, and their imaging manifestations can be similar.It is clinically difficult to distinguish them from other lesions such as bullae, cyst, and emphysema.Some scholars have advanced a concept about thin wall(4 mm or less) and thick wall(more than 4mm).People tried to make this distinction by defining cyst as a thin wall and cavity as a thick wall, but there are considerable overlaps between the two categories in etiology and pathophysiology.They are sometimes difficult to distinguish for imageology, and it is still necessary to find the cause of the disease based on the characteristics.This review divides etiology into two categories: infectious and non-infectious etiology.Combined with chest imaging examination, the purpose is to analyze and summarize the features of pulmonary cavitary lesions in children, and provide a diagnostic idea for differentiating various pulmonary cavities to guide clinical treatment.

Objective To explore the clinical curative effect of traditional Chinese medicine enema,amino salicylic acid preparation and probiotics in the treatment of ulcerative colitis.Methods 168 patients were selected as the research objects which were confirmed by endoscopic examination and the diagnosis of ulcerative colitis patients,randomly divided into the control group of 84 cases and observation group of 84 cases.The control group was given with sulfasalazine(SASP) treatment,a dose of 1g,4 times/d,period of treatment was 28d.In the observation group,oral SASP treatment combined with microecological preparation often meian and traditional Chinese medicine enema,Chang An capsule,2 capsules (500mg)/time,3 times/d,postprandial oral; Herbal Enema with angelica,white lentils,flavescent sophora root,cicada,windproof suffering after filtering,every night before bed retention enema.The clinical efficacy was compared between the two groups.Results After the treatment,the Sutherland index of the observation group was (1.82 ± 1.65),which was significantly lower than (5.51 ± 1.59) of the control group (t =10.436,P ＜ 0.05).In observation group,the clinical total effective rate,the total efficiency of endoscopic and histological total effective rate was 88.1％,81％,76.2％,the control group were 66.7％,57.1％,50％,the two groups had significant difference(x2 =4.353,4.512,5.113,P＜0.05).Conclusion The traditional Chinese medicine enema,amino salicylic acid preparation and probiotics in the treatment of ulcerative colitis has good curative effect,can relieve the clinical symptoms of patients,improve the degree of intestinal diseases.

Haploidentical hematopoietic stem cell transplantation (Haplo-HSCT) has been an alternative source of stem cell transplantation,the successful application of which makes each transplant candidate has his/her donor.In this paper,advances in the Haplo-HSCT reported in the 56th American Society of Hematology (ASH) annual meeting are reviewed.

Objective To evaluate of the disability adjusted life years (DALY) and its influencing factors in patients with epilepsy.Methods Eight hundred and seventy-four cases with epilepsy were investigated in 9 pilot sites in Henan province.Make up the life expectancy of residents in Henan province for the DALY estimates.Using the R 2.10.0 software,the quality-adjusted life years and its influencing factors were analyzed by univariate and multivariate analyzes.Results There was 16.64 per capita loss of all cases of epilepsy DALY,and DALY loss value was 3.41 per 1000 populations.Comprehensive intervention and no sense of control group patients were per capita DALY loss of 8.56 person-years and 24.19 person-years respectively.There was significant difference between the two groups DALY loss (Z =23.146,P ＜ 0.01).Moreover,non-conditional Logistic regression equation was also statistically significant (x2 =365.83,P ＜ 0.01).Age,duration,seizure frequency,compliance,and anxiety situation were the main factors of the disease burden.Conclusions In the future,enhanced interventions should be focused on the priority groups and the modifiable factors.Additionally,the ‘standard treatment + follow-up management and health education + psychological intervention' should be strengthened as a primary means of comprehensive intervention.

Objective To investigate expression level of sphingosine kinase 1 (SPHK1) and nuclear factor-κB (NF-κB) in non-small cell lung cancer (NSCLC) and their relationships with invasion, metastasis and prognosis of NSCLC. Meth-ods Ninety-three NSCLC specimens and paraneoplastic normal lung tissue from conventional surgery were confirmed by histology. Expression of SPHK1 and NF-κB were detected by Immunohistochemistry on paraffin sections. Primary antibody were Rabbit Anti-Human SPHK1 and Rabbit Anti-Human NF-κB p65, which were incubated 1 hour in water bath. The secondary antibody was HRP-Polymer anti Mouse IgG, which was incubated 20 minutes in water bath. Results SPHK1 ex-pression was positive in 96.8% (90/93) of NSCLC specimen which is higher than in paraneoplastic normal lung tissue in which the positive rate is 18.3%(17/93);NF-κB expression was positive in 89.2%(83/93) NSCLC which is higher than the in paraneoplastic normal lung tissue in which the positive rate is 12.9%(12/93). The expression of SPHK1 and NF-κB in NSCLC was positively correlated (r=0.464, P＜0.01). TThe expression levels of SPHK1 and NF-κB p65 in NSCLC patients with were positively related to TNM staging and lymph node metastasis. SPHK1 expression and NF-κB p65 expression lev-el were higher in the deads than in survivals. There was no statistical significance in different expression intensity of SPHK 1 and NF-κB p65 in patients with NSCLC who had differences in gender, age, tumor size, tumor location, histological type. Survival analysis showed that survival time of patients of NSCLC with high expression of SPHK1 was shorter than those in the group with low SPHK1 expression, and the difference was statistically significant (χ2=14.025, P ＜ 0.01). Conclusion In the process of NSCLC invasion and metastasis,SPHK1 may play an important role through NF-κB, and it can predict prognosis of NSCLC patient. Moreover, it will become a potential target for NSCLC target.

Objective: To validate the therapeutic value of CT-guided percutaneous ethanol injection (PEI) in the treatment of metastatic adrenal tumors. Methods: Thirty one foci (diameter ranging from 1.5 cm to 7.2 cm) in 25 patients with metastatic adrenal tumors were treated with CT-guided PEI for more than twice. Plain and enhanced CT scans were performed 1-2 months after PEI to observe the size and necrosis of the tumors. Results: Post-PEI enhanced CT scan showed that 18 of the 20 foci with diameters less than 3 cm completely necrotized; 11 foci with diameters between 3 cm and 7.2 cm necrotized partially, and 2 foci completely necrotized after another 2-3 courses of PEI treatment. Conclusion: CT guided PEI is a simple and minimally invasive means for treatment of metastatic adrenal tumors, and the therapeutic effect is satisfactory.