OBJECTIVE: The kinetic indices of apoptosis and cell proliferation m a histopathologic spectrum of the cervical neoplasia were evaluated to clarify the correlation between cervical neoplasm and apoplasis. Specific lesioas included cervical intraepithelial neoplasia(CIN), catcinoma in situ(CIS), and invasive carcinama. METHODS: Archival samples from normal cervical epithelium(n=7), low-grade squamous intraepithelial lesions(LGSIL, n=17), high-grade squamous intraepithelial lesions(HGSIL, n=17), invasive squamaus carcinoma(n=7) were evaluated for apoptosis and cell proliferation. Apoptotic cells were identified with terminal deoxynucleotidyl transferase-labeling of the 3'-OH end of DNA nucleosomes, and then apoptotic index(A.I sum of apoptotic bodies/ 1000 tumor cells) and total cell count(* 400 magnification) were calculated. RESULTS: In normal squamous epitheliam, Apoptotic bodies were mainly localized in ial layers, for low-grade squamous intraepithelial lesions(HGSIL) in superficial and intermediated layers, for high-pade squamous intraepithelial lesions(HGSIL) in intermediated and parabasal layers, for invasive carcinoma in full thic of squamous epithelium. Apototic indces(AI) in invasive carcinoma(mean: 6.21) were significantly higher than indices for high-grade squamous intraepithelial lesions(HGSIL, mean: 0.98) and high-grade squamous intraepithelial lesions(HGSIL, mean: 0.98) were significantly higher than indices for low-grade squamous intraepithelial lesions(LGSIL, mean: 0.12)(p<0.01), the total cell counts increased significantly as the specimens progressed toward invasive disease. (p<0.01) CONCLUSION: Apoptosis in cervical neoplasia appears to be closely related to poliferation and progression of the cervical squamous epithelial cell. This phenotype may allow identification of premalignant lesions with the potential to transform to cervical cancer.
Apoptosis* ; Cell Count ; Cell Proliferation ; DNA ; Epithelial Cells ; Epithelium ; Nucleosomes ; Phenotype ; Uterine Cervical Neoplasms

No abstract available.

A statistical comparison of pulmonary artery and pulmonary venous wedge pressure has been made by the correlation coefficient method in 24 children with various congenital heart disease. None of them had pulmonary hypertension above the normal range. During the systolic phase, pulmonary arterial pressure was 2.02+/-2.64mmHg greater than pulmonary venous wedge pressure with poor correlation(r=0.57). During the diastolic phase, pulmonary venous wedge pressure was 2.08+/-2.47mmHg greater than pulmonary aetery pressure with poor correlation(r=-.63). Mean pulmonary arterial pressure was 0.79+/-1.02mmHg greater than pulmonary venous wedge pressure with good correlation (r=0.96). Therefore, it is concluded that if pulmonary artery is not entered, a pulmonary vein wedge pressure is a useful indication of pulmonary artery mean pressure in selected cases of congenital heart disease.
Arterial Pressure ; Child ; Heart Defects, Congenital* ; Humans ; Hypertension, Pulmonary ; Pulmonary Artery* ; Pulmonary Veins ; Pulmonary Wedge Pressure* ; Reference Values

OBJECTIVE: Frozen-section evaluation of gynecologic tumors has historically been used to establish a histopatholgic diagnosis and to guide surgeons in appropriate surgical procedure. this study is to assess the clinical appropriation and accuracy of frozen-section biopsy in gynecologic tumors. METHODS: This is a retrospective quality assurance study all of frozen-section biopsy done at Chungnam National University Hospital during a three year period (1995 to 1997). There were 125 cases out of 2310 surgical specimens for gynecologic tumors, and the results were compared with final diagnoses based on fixed tissue specimens. RESULTS: Frozen section biopsy was accurate in 95.2% of all cases, in 96.3% (104/108) of ovarian tumor cases, and in 88.2% (15/17) of uterine tumor cases. Analysis of 6 inaccuracy cases revealed that most cases were sampling errors and occurred in mucinous tumor, belonging to all cases of ovarian tumors. Sensitivity, specificity, positive predictive value, and negative predictive value of frozen-section biopsy in ovarian tumors were 0.94, 1.00, 1, 00, 0.97. CONCLUSION: Frozen section biopsy is an appropriate method as a basic guideline during operations of gynecologic tumors and this method can obtains the highest accuracy when there is cooperation between experienced surgeons and reliable and careful pathologists. The accuracy of frozen section diagnosis has important implications regarding the type and extent of the primary surgery for gynecologic tumors.
Biopsy* ; Chungcheongnam-do ; Diagnosis* ; Frozen Sections* ; Mucins ; Retrospective Studies ; Selection Bias ; Sensitivity and Specificity

Baker's cyst is found as most frequent cystic mass around the knee occurring between medial head of gastrocnemius muscle and semimembranosus muscle. The proximal or posterolateral extension of the cyst had been rarely reported and the cyst into the surrounding muscular tissue extremely rare. Intramuscular Baker's cyst that we report was found between lateral head of gastrocnemius muscle and popliteus tendon, and then extended into the plantaris muscle. With review of the literature, we here report a very rare case of plantaris muscular extension of Baker's cyst.
Head ; Knee ; Muscle, Skeletal ; Muscles ; Popliteal Cyst ; Tendons

No abstract available.
Macrophages*

No abstract available.
Macrophages*

No abstract available.
Sterilization, Tubal*

No abstract available.
Blood Pressure* ; Child* ; Humans ; Seoul*

No abstract available.
Splints* ; Sterilization, Tubal*