No abstract available.
Hepatitis B* ; Hepatitis*

Due to the limited availability of immunoelectron microscopy, an alternative method for the differentiation between anti-lamina lucida and anti-sublamina densa antibodies was introduced; indirect immunofluorescence using NaCl-treated human skin as the substrate. In this study author examined sera and lesional skin of 4 cases of bullous pemphigoid (BP), and 2 cases of epidermolysis bulloaa acquisita(EBA) with the above mentioned indirect imrnunofluorescence and modified direct immunofluorescence to evaluate the specificity of the tests. The results showed that in BP the fluorescence patterns were epidermal in 3 patients with 1 combined by indirect immunofluorescence, and epidermal in all 4 patients by modified direct immunofluorescence. In ERA the fluorescence were dermal patterns in both 2 patients by indirect and modified direct immunofluorescence. These data are further confirming the syecificity and the reproducibility of the NaCl extraction technique for the irnmunofluorescence to differentiate the localization of the autoantibodies in the above two bullous dermatoses.
Antibodies ; Autoantibodies ; Epidermolysis Bullosa Acquisita* ; Epidermolysis Bullosa* ; Fluorescence ; Fluorescent Antibody Technique ; Fluorescent Antibody Technique, Direct ; Fluorescent Antibody Technique, Indirect ; Humans ; Microscopy, Immunoelectron ; Pemphigoid, Bullous* ; Sensitivity and Specificity ; Skin ; Skin Diseases, Vesiculobullous

No abstract available.

No abstract available.
Hepatitis, Chronic* ; Korea* ; Lamivudine*

No abstract available.
Japan*

No abstract available.
Japan*

No abstract available.
Lung Neoplasms* ; Lung*

No abstract available.
Hepatitis B, Chronic* ; Hepatitis, Chronic*

No abstract available.

Immunologic or immunopathologic assays are neccesary for the diagnosis of autoimmune bullous dermatoses including pemphigus vulgaris (PV), bullous pemphigoid (BP), and epidermolysis bullosa acquisita (EBA). The objectives of this study is to compare the sensitivity and usefulness of indirect immunofluorescence 0F) with that of immunoblot assay using amplified alkaline phosphatase staining system in the diagnosis of the above diseases; detection of disease-specific IgG autoantibodies. We selected 4 patients in each bullous dermatosis of PV, BP, and EBA, who had serum levels of IgG autoantibodies at a titer of 1:80 or higher. In each three disease, 2 patients with negative serum antibodies or serum titer lower than 1:20, were also enrolled. Among the former 4-patient groups the titers of IgG antibodies found on indirect IF were in the range of 1:80 to 1:160, whereas the titers recognized by immunoblot assay were 1 or 2 dilutions higher in most of these patients. In the latter 2-patient groups, 4 out of the 6 cases revealed antibody-positive on immunoblot-staining membrane. The indirect IF can be performed easily and seems favorable in the aspect of cost-effectiveness. However, immunoblot assay with sensitive staining method would be warranted in cases of antibody-negative or atypical clinical variants of autoimmunebullous dermatoses to confirm the diagnosis.
Alkaline Phosphatase ; Antibodies ; Autoantibodies ; Diagnosis ; Epidermolysis Bullosa Acquisita ; Fluorescent Antibody Technique, Indirect ; Humans ; Immunoglobulin G ; Membranes ; Pemphigoid, Bullous ; Pemphigus ; Skin Diseases ; Skin Diseases, Vesiculobullous*