No abstract available.
Animals ; DNA* ; LLC-PK1 Cells* ; Swine

No abstract available.

The object of this study was 336 Korean patients with degenerative arthritic knees and 1000 Koreans with normal knees who had visited the department of orthopaedic surgery of Hanyang University hospital during the period fmm January, 1980 to June, 1981. The purpose of this study was to investigate the changes of tibio-femoral angles and metaphysealdia physeal angles in response to the degenerative changes of the knees. By the roentgenogram, tibio-femoral angles and metaphyseal-diaphyseal angles were measured and those angles were analized statistically. The results were as follows 1. In young age group, the incidence of the degenerative arthritic knees was more frequent in male than in female. However, in the middle and older age groups, the incidence was higher in female than in male. 2. The mean value of tibio-femoral angle of the degenerative arthritic knees was −3.41°±0.22. It was about 1° varus compared with the normal mean value of −4.46°±0.27. The mean value of tibio-femoral angle in normal Korean was reduced appmximately 3°±5°valgus compared with the Westem normal peoples: 3. In general, the valgus tendency appeared a little more in the knees of female than in those of male. 4. The varus tendency was in pmportion to the degree of the aggrevation of the degenerative arthritis in the knees. 5. The mean value of metaphyseal-diaphyseal angle in normal Korean was 3.53°±0.24. It was 3°–4° varus compared with the Western normal peoples. 6.The varus was increased more in the standing position, in the state of weight bearing position, than in the supine position.
Female ; Humans ; Incidence ; Knee ; Male ; Osteoarthritis ; Posture ; Supine Position ; Weight-Bearing

No abstract available.
Adult* ; Humans ; Molecular Epidemiology* ; Oxalobacter formigenes* ; Seoul*

PURPOSE: To evaluate the efficacy of combined treatment of metallic biliary stent placement and radiotherapy in malignant biliary obstruction. MATERIALS AND METHODS: Between January 1992 and February 1994, 22 patients with malignant biliary obstruction were treated with metallic biliary stent placement and radiotherapy. The causes of the obstruction included cholangiocarcinoma(n=14), gallbladder carcinoma(n=4), colon cancer(n=2), pancreatic head cancer (n=1), and stomach cancer(n=1). According to the sites of obstruction level, patient were divided into 2 groups; hilar obstruction(n=18) and common bile duct obstruction(n=4). The patients received dose of 45 Gray/18 fraction by external radiotherapy and 20Gy/8f by high dose rate brachytherapy with iridium--192 source through percutaneous transhepatic biliary drainage(PTBD) catheter. The duration of stent patency after placement, survival period and survival rate by Kaplan Meier method were calculated in dead and alive patients, respectively. RESULTS: In all 22 patients, the duration of stent patency was 5.5(1.3--18.5)months. Survival periods after stent placement and PTBD were 5.3(2.0--8.5 )months and 7.9(4.0--14.7)months respectively in 9 dead patients and 7.7(1.3--21.0)months and 9.5(2.3--23.0)months in 13 alive patients. In all 22 patients, the survival rates in 6, 12 and 18 months were 78.9%, 47.5 % and 35.6 %, respectively. CONCLUSIONS: The results with combined metallic biliary stent placement and radiotherapy for the palliative treatment of malignant biliary obstruction in this study was more favorable, as compared with those of the other authors with only metallic biliary stent placement.
Brachytherapy ; Catheters ; Colon ; Common Bile Duct ; Gallbladder ; Head and Neck Neoplasms ; Humans ; Palliative Care ; Radiotherapy* ; Stents* ; Stomach ; Survival Rate

Free dorsalis pedis flap transfers were performed in twelve cases at the Department of Orthopaedic Surgery of Hanyang University Hospital from January, 1980 to December, 1983. The result were summerized as followings; 1. Among twelve cases of the free dorsalis pedis flap transfer, neurovascular flap transfers were performed in nine cases and tendocutaneous flap transfers in five cases. 2. In all cases the textures of flaps were improved and the bulky subcutaneous fat tissues were shrunk gradually, so cosmetically good results were obtained. 3. Temperature, pain, protective and touch sensations were retained or restored by preservation of sensory nerve. 4. Free dorsalis pedis flap transfer has many advantages compared to conventional skin grafts, such as shorter therapeutic time, lesser physical or economic demands and primary covering to vital organ. 5. In injured hands, the application of free dorsalis pedis flap transfer including long extensor tendons of foot has been shown the excellent clinical result in the point of functional and cosmetic effect in dorsum of hand, as transferring simultaneously free tendon and skin flap. 6. Free dolis pedis flap transfer needs abundant experiences, meticulous microvascular technique and anatomic knowledge.
Clinical Study ; Foot ; Hand ; Microsurgery ; Sensation ; Skin ; Subcutaneous Fat ; Tendons ; Transplants

The effect of acetabular augmentation with bone graft was reviewed in 12 patients with acetabular deficiency in total hip arthroplasty, performed between 1981 and 1984 at Hanyang University Hospital. Follow-up ranged from 12 to 18 months and averaged 23.3 months. Follow-up radiographs were analyzed. Based on radiography, all grafts were regarded as incorporated and equally successful with autogenous and allograft bone. Long-term follow-up evaluation revealed no evidence of progressive radiolucency or graft resorption. There was one sciatic nerve palsy after operation but recovered at 11 months later.
Acetabulum ; Allografts ; Arthroplasty, Replacement, Hip ; Follow-Up Studies ; Humans ; Radiography ; Sciatic Neuropathy ; Transplants

PURPOSE: Nanobacteria have been reported to induce various pathologic calcifications like atherosclerosis and nephrolithiasis, and they do so by forming an apatite envelope, however, this concept is still controversial. We tried to elucidate whether nanobacteria might be related with urinary calcium stone by performing comparative study. MATERIALS AND METHODS: This study included 38 urinary stone patients who were proved to have calcium-containing stones and 37 healthy adults without urinary stone disease as controls. The subjects' age and gender were well matched between both groups. For the detection of nanobacteria, the serum and urine of all subjects were collected and western blotting for the samples was performed. RESULTS: There was no significant difference in the positive rate of nanobacteria from the serum samples between stone and control groups (52.6% vs 48.6%, respectively, p=0.465). But on the urine samples, the stone group showed a significantly higher positive rate than the control group (71.1% vs 21.6%, respectively, p<0.05). CONCLUSIONS: Nanobacteria might have a relation with urinary calcium stone disease.
Adult ; Atherosclerosis ; Bacteria ; Blotting, Western ; Calcifying Nanoparticles* ; Calcium* ; Humans ; Nephrolithiasis ; Urinary Calculi ; Urolithiasis

We evaluated the role of retroperitoneal laparoscopic ureterolithotomy (RPLU) for upper ureter stones. Between February 1998 and July 2004, 12 patients (10 men and 2 women) underwent RPLU for upper ureter stones (mean size 18.1 mm, range 10-25). RPLU was carried out in 5 patients as a salvage treatment after failed shock wave lithotripsy (SWL) (3) and both of failed SWL and ureteroscopy (URS) (2), and in 7 patients as primary treatment for large impacted stones. Total 6 of 12 cases were converted to open surgery. The reason of open conversion was failure of locating ureter due to severe adhesion in 5 cases and vascular injury in 1 case. In 6 successful cases, mean operation time, mean estimated blood loss, and mean postoperative hospital stay were respectively 109 min (90-120 min), 50 mL (10-100 mL), 4.6 days (2-7 days). There was no serious postoperative complication except for one patient who showed delayed urinary leakage but was cured with conservative management. Our experience suggested that RPLU was not easy to perform simply because it was indicated mainly in ureter stones with severe adhesion or after failed SWL and/or URS. Nevertheless, it can be considered as a primary procedure before open ureterolithotomy.
Urologic Surgical Procedures/*methods ; Ureteral Calculi/*therapy ; Ureter/pathology ; Treatment Outcome ; Time Factors ; Sex Factors ; Retroperitoneal Space/pathology ; Peritoneum ; Middle Aged ; Male ; Lithotripsy/*methods ; Laparoscopy/*methods ; Humans ; Female ; Adult

PURPOSE: Recently, the whole DNA sequence of Bacillus subtilis (B. subtilis) was identified, revealing the existence of the YvrK gene encoding a 43 kD oxalate decarboxylase (OXDC), which degrades oxalate by a simple pathway. The objective of this study was to develop recombinant Escherichia coli (E. coli) expressing the Yvrk gene from B. subtilis. MATERIALS AND METHODS: After the extraction of total DNA from B. subtilis, the YvrK gene was cloned by polymerase chain reaction. The cloned DNA encoding OXDC was inserted into the pBAD/gIII-A vector, downstream of the L-arabinose promotor. The plasmid vector was transformed into TOP 10 E. coli, and the transformants were selected with ampicillin. The recombinant E. coli, named pBy, was then analyzed by DNA sequencing and Western blot. To evaluate the oxalate-degrading function of pBy, pBy was cultured in LB broth containing oxalate, and then the amount of oxalate in the medium was assessed. The oxalate-degrading activity of homogenates of pBy was evaluated. RESULTS: DNA sequencing showed the successful transformation of the YvrK gene into TOP 10 E. coli. Western blot analyses showed that pBy expressed OXDC. pBy removed oxalate during the overnight culture in oxalate-containing LB broth, and the homogenate of pBy degraded 90% of oxalate under acidic conditions. CONCLUSIONS: A recombinant E. coli expressing the YvrK gene was successfully produced. The bacteria showed potent oxalate-degrading activity. The results of this study will provide a solution to the treatment of calcium oxalate stones and hyperoxaluria, for which there are few medical treatment modalities.
Ampicillin ; Arabinose ; Bacillus subtilis ; Bacteria ; Base Sequence ; Blotting, Western ; Calcium Oxalate ; Carboxy-Lyases ; Clone Cells ; DNA ; Escherichia coli ; Hyperoxaluria ; Oxalates ; Plasmids ; Polymerase Chain Reaction ; Sequence Analysis, DNA