No abstract available.
Child* ; Humans

BACKGROUND: As more than 80% of Korean hospital laboratories don't use glycerol blank for the triglyceride measurement, we investigated free glycerol interference on the measurement of triglyceride concentration. METHODS: We collected 237 specimens which had more than 300mg/dL of triglyceride measured by Abbott (without glycerol blank, USA) reagent from the 217 patients visiting Catholic University Hospital of Taegu Hyosung from September, 1995, to May, 1996. We retested them with Youngdong (without glycerol blank, Korea) reagent, and IRC reagent (with glycerol blank, Japan), and also measured free glycerol concentration with Youngdong reagent. Then we examined the clinical records of the patient showing increased glycerol concentration. RESULTS: Average triglyceride concentration measured by Abbott reagent was 448.0+/- 165.9 mg/dL and average glycerol interference to triglyceride concentration was 3.4+/- 6.7%, There were 8 patients (3.4%) who revealed more than 10 % of glycerol interference. Except these patients the average glycerol interference was 2.4+/- 1.4%. Among these 8 patients, 3 patients were treated with Frucenil and other 3 were with Intralipose(R), and the other 2 were diagnosed as diabetes and preeclampsia respectively. In vitro test, Frucenil mixed with serum in 1% (v/v) increased triglyceride concentration as high as 640.6% with Abbott reagent, but it did only 48.4% with IRC reagent. CONCLUSIONS: Triglyceride measurement by non glycerol blank method exceeded the recommended limit of National Cholesterol Education Program's precision, and this method extremely overestimated the triglyceride concentration for the patient with the glycerol containing fluid therapy. So all laboratories would be encouraged to use the glycerol blank method for triglyceride measurements.
Cholesterol ; Daegu ; Education ; Fluid Therapy ; Glycerol* ; Humans ; Laboratories, Hospital ; Pre-Eclampsia ; Triglycerides*

BACKGROUND: As more than 80% of Korean hospital laboratories don't use glycerol blank for the triglyceride measurement, we investigated free glycerol interference on the measurement of triglyceride concentration. METHODS: We collected 237 specimens which had more than 300mg/dL of triglyceride measured by Abbott (without glycerol blank, USA) reagent from the 217 patients visiting Catholic University Hospital of Taegu Hyosung from September, 1995, to May, 1996. We retested them with Youngdong (without glycerol blank, Korea) reagent, and IRC reagent (with glycerol blank, Japan), and also measured free glycerol concentration with Youngdong reagent. Then we examined the clinical records of the patient showing increased glycerol concentration. RESULTS: Average triglyceride concentration measured by Abbott reagent was 448.0+/- 165.9 mg/dL and average glycerol interference to triglyceride concentration was 3.4+/- 6.7%, There were 8 patients (3.4%) who revealed more than 10 % of glycerol interference. Except these patients the average glycerol interference was 2.4+/- 1.4%. Among these 8 patients, 3 patients were treated with Frucenil and other 3 were with Intralipose(R), and the other 2 were diagnosed as diabetes and preeclampsia respectively. In vitro test, Frucenil mixed with serum in 1% (v/v) increased triglyceride concentration as high as 640.6% with Abbott reagent, but it did only 48.4% with IRC reagent. CONCLUSIONS: Triglyceride measurement by non glycerol blank method exceeded the recommended limit of National Cholesterol Education Program's precision, and this method extremely overestimated the triglyceride concentration for the patient with the glycerol containing fluid therapy. So all laboratories would be encouraged to use the glycerol blank method for triglyceride measurements.
Cholesterol ; Daegu ; Education ; Fluid Therapy ; Glycerol* ; Humans ; Laboratories, Hospital ; Pre-Eclampsia ; Triglycerides*

No abstract available.

No abstract available.
Humans ; Intensive Care Units* ; Critical Care* ; Korea*

No abstract available.
Alloxan* ; Animals ; Rats*

The purpose of this study was to investigate the morphologic changes of the bile canaliculi and its associated structures of the liver induced by common bile duct ligation(CBDL) in the rat. The canalicular surface and lateral surface of the dry-fractured hepatocytes was studied with scanning electron microscopy at 1~6 weeks post ligation. The first week after CBDL, the bile canaliculi were dilated. The microvilli were increased in number and the lumens contained granular materials After 2 weeks or more, the bile canaliculi were dilated to a variable degree, and with irregularity, measuring from 1.5 to 5 micrometer in diameter, and in the advanced stage, the canaliculi showed blunting and the disappearance of microvilli. Some canaliculi had sprouting side branches. At 4~6 weeks post-ligation, the lateral surface of the hepatocytes also showed some irregularity and a tortuous appearance, and numerous small sized microvillous projections were formed. The tubular structures of the proliferated SER distributed adjacent to the lateral surface of the hepatocytes, and the direct connection of a tubular structure and the cytoplasmic membrane was observed. These results suggest that the deformity and loss of microvilli of bile canaliculi reflect the disturbance of bile secretion from the hepatocytes. And prolonged obstruction of bile flow may result in bile excretion via the lateral surface of hepatocytes.
Rats ; Animals

Perioperative surgical stress makes various metabolic and endocrinologic changes that may influence the postoperative outcome. These changes, so-called, "surgical stress responses" ar hyperglycemia, lipolysis, negative nitrogen balance due to proteolysis, and changes in the hormonal secretion, e.g. increased release of ACTH, prolactin, cortisol, catecholamine, and so on. To prevent these stress responses, many studies reported some kinds of effective methods. In these study we used general anesthesia combined with epidural blockade with local anesthetic(bupivacaine) and narcotic(fentanyl) mixture via epidural catheter for the total abdominal hysterectomy and the postoperative pain control(group II). We measured serum prolactin and cortisol concentrations by time-resolved fluoroimmunoassay and glucose concentrations by routine glucose oxidase method and scored the degree of postoperative pain and compared the results with those of group I, given general anesthesia during surgery and nalbuphine intramuscular injection for postoperative pain control. The results were as follows. 1) Serum prolactin concentrations were increased during the operation in both groups. 2) Serum cortisol concentrations were increased during and after the operation in both groups but were normalized sooner in group II. 3) Serum glucose concentrations were increased during and after the operation but were normalized sooner in group IL 4) The three measurements showed significant differences between the two groups during and on the first postoperative day. 5) The pain scores were significantly lower in group II untill the third postoperative day. According to the results, we concluded that serum prolactin concentration could be as much valuable index to surgical stress response as serum cortisol concentration and that the method of epidural blockade combined with general anesthesia for the surgery and postoperative pain control via epidural route could prevent or alleviate the stress response more effectively.
Adrenocorticotropic Hormone ; Anesthesia, Epidural* ; Anesthesia, General* ; Blood Glucose* ; Catheters ; Fentanyl* ; Fluoroimmunoassay ; Glucose ; Glucose Oxidase ; Hydrocortisone* ; Hyperglycemia ; Hysterectomy ; Injections, Intramuscular ; Lipolysis ; Nalbuphine ; Nitrogen ; Pain, Postoperative ; Prolactin* ; Proteolysis

Most renal cell carcinomas are solid but contain small cystic areas. Sometimes the cystic component predominates and such lesions are called cystic renal cell carcinomas. The radiographic and pathologic findings of cystic renal cell carcinoma are often more confusing and less specific than the findings of solid renal cell carcinoma. Multilocular cystic renal cell carcinoma is one form of cystic renal cell carcinoma variants and results from intrinsic multiloculated growth. A presumptive diagnosis of multilocular cystic renal cell carcinoma was made preoperatively in a 38-year-old woman and it was treated with radical nephrectomy, so we report this case with brief review of the literatures.
Adult ; Carcinoma, Renal Cell* ; Diagnosis ; Female ; Humans ; Nephrectomy

A study to evaluate the diagnostic significance of M. pneumoniae Infection by measurements of cold agglutinin and antimycoplasma antibody titers is performed with 191 pediatric patients who have visited Yeungnam University Hospital during the period through January to July, 1987. Forty eight of 191 cases made follow up tests feasible. The results obtained are as follows: 1. It is necessary to perform routine combined measurements of cold agglutinin and antimycoplasma antibody titers for the all pediatric pneumonia caser since a large proportion of pneumonia in children is caused by M. pneumonia. 2. For the diagnosis of M. pneumoniae Infection, measurements of cold agglutinin titer alone seems to be less significant than to check both cold agglutinin and antimycoplasma antibody titers. 3. The measurement of antimycoplasma antibody titer appeared to be more specific than cold agglutinin test in the diagnosis of M. pneumoniae Infection. 4. The present study urges the necessity of follow up study of cold agglutinin and antimycoplasma antibody titer for those who initially presented with normal titers in both tests, but are clinically suspected for M. pneumoniae Infection.
Child ; Diagnosis ; Follow-Up Studies ; Humans ; Mycoplasma pneumoniae* ; Mycoplasma* ; Pneumonia ; Pneumonia, Mycoplasma*