An activation domain in p67(phox) (residues 199-210) is critical for regulating NADPH oxidase activity in cell-free system [10] To determine the steady state reduction of FAD, thioacetamide-FAD was reconstituted in gp91(phox), and the fluorescence of its oxidised form was monitored. Omission of p67(phox) decreased the steady state reduction of the FAD from 28% to 4%, but omission of p47(phox) had little effect. A series of the truncated forms of p67(phox) were expressed in E.coli to determine the domain in p67(phox) which is essential for regulating the steady state of FAD reduction. The minimal length of p67(phox) for for regulating the steady state of FAD reduction is shown to be 1-210 using a series of truncation mutants which indicates that the region 199-210 is also important for regulating electron flow within flavocytochrome b(558). The deletion of this domain not only decreased the superoxide generation but also decreased the steady state of FAD reduction. Therefore, the activation domain on p67(phox) regulates the reductive half-reaction for FAD, consistent with a dominant effect on hydride/electron transfer from NADPH to FAD.
Amino Acid Sequence ; Base Sequence ; Cell Membrane/metabolism ; Cell-Free System ; DNA Primers ; Flavin-Adenine Dinucleotide/*metabolism ; Humans ; Kinetics ; Membrane Glycoproteins/*metabolism ; Molecular Sequence Data ; NADH Dehydrogenase/metabolism ; *NADPH Oxidase ; Neutrophils/enzymology/metabolism ; Oxidation-Reduction ; Peptide Fragments/chemistry ; Phosphoproteins/*chemistry/*metabolism ; Polymerase Chain Reaction ; Sequence Deletion

No abstract available.
Hand*

The displacement of patellar components, such as dislocation or subluxation, causes polyethylene wear and further induces metallosis of the metal-backed patella. We compared two different patellar components, onset(Miller-Galante I) and inset(Whiteside-Ortholoc I)type, to understand the differences in patellar tracking according to their designs. Patellar prosthesis position in 43 primary total knee arthroplasties was evaluated with patellar tilt, patellar displacement from the center of the trochlear groove, medial placement, patellar thickness, and joint line height. For the MG(Miller-Galante I)group, the patellar displacement was laterally 50.0% and for the Ortholoc group, it was 33.3%. In the MG group, 4 cases of revision of the patellar component due to excessive polyethylene wear were performed, but there was no excessive wear of polyethylene in Ortholoc group. One of the primary factors which influence tracking of the patellofemoral joint is the difference in design between the patellar component and the femoral component. But in the opinion of the authors, the slope or lateral displacement of the patellar component have no correlation with each other under radiological measurements. However, the inset-type patellar component is a design that maintains the bony flange within the lateral flange, therefore relieving the shear stress and rotational forces loaded on the patellofemoral joint and protecting the metal-backing from exposure. Thereby, even if dislocation or subluxation of the patellar component should occur, polyethylene wear or metallosis caused by excessive wearing can be minimal.
Arthroplasty* ; Dislocations ; Joints ; Knee* ; Patella ; Patellofemoral Joint ; Polyethylene ; Prostheses and Implants

Early diagnosis and treatment of congenital dislocation of the hip is the only means of obtaining good results with consistent regularity. But a large number of congenital dislocation of the hip remain undiagnosed before the child begins to walk. One hundred and nine cases of congenital dislocation of the hip had been clinically diagnosed and 48 cases of them were treated conservatively or operatively at Department of Orthopedic Surgery, College of Medicine, Seoul National University from January, 1965 through September, 1975 with following results. 1. Among 109 cases of congenital dislocation of the hip, 48 cases were admitted and treated. In recent 10 years the number of the out-patient was increasing. 2. The preponderance of female to male (4:1) was found. 3. The age ranged between 3 months and 24 years, and 46 cases (42%) between 12 and 24 months. 4. In most cases the chief complaint was limping. 5. The associated congenital anomalies were observed in 3 cases of the series, comprising two cases of arthrogryposis multiplex congenita and one case of hydrocephalus. 6. The scetabular index was increased in affected hip. 7. The ratio of unilateral cases to bilateral ones was 100: 9, and right to left was 47: 53. 8. Closed reduction was satisfactory in 25 cases under 2 years of age. 9. Open reduction was necessary in 18 cases of failed closed reduction and 5 cases of redislocation. 10. Eleven cases of Salter's operation, 4 cases of Pembertons operation, 2 cases of Colonnas operation, and 8 cases of derotational osteotomy were performed to increase the stability of the hip joint.
Arthrogryposis ; Child ; Dislocations ; Early Diagnosis ; Female ; Hip Joint ; Hip ; Humans ; Hydrocephalus ; Male ; Orthopedics ; Osteotomy ; Outpatients ; Seoul

Patients with rickets frequently are seen by orthopaedic surgeon with complaints of growth disturbance, limb deformity, weakness, and multiple fracture. Among them, bow leg deformity is the most common and difficult orthopaedic problem. In growing child, however, if effective tretment is given sufficiently in early age and early disease process, the deformities usually correct spontaneously and there is little need for orthopaedic treatment other than careful observation. The orthopaedic treatment may be required to correct deformities that cannot be expected to improve with growth. A clinical study was done for 11 cses of rachitic bow leg deformity who were treated at the department of orthopaedic surgery, Catholic medical College and Center from Jan. 1978 to Dec. 1981. Results obtained were as follows: 1. The most common patients age group was in 1 to 2 years of age and there was no sexual difference. 2. Associated deformities with rachitic bow leg were rachitic rosary (5 cases), double wrist (3 cases), coxa vara (1 case), and Harrison's groove (1 case). 3. Low Ca * P solubility product, below the level of 30 (mg/dl)2 suggested active form of rickets in all cases. 4. Laboratory values such as Ca * P solubility product and alkaline phosphatase improved at 3 weeks after administration of vitamin D in 7 cases, 3 at 6 weeks, and no improvement in one case even at 6 months after treatment. 5. Six months after treatment, the average amount correction of tibiofemoral angle was 9° (43.7% of initial angle) in group 1, 13.3° (47.0%) in group II, 4.9° (29.6%) in group III, and 3.3°(25.6%) in group IV. 6. Through this study it is suggested that the more growth correction of the rachitic bow leg deformity in a younger child below the age of 2 is obtained with growth by treatment, whereas a little or no growth correction can be expected after the age of 3 or 4 years.
Alkaline Phosphatase ; Child ; Clinical Study ; Congenital Abnormalities ; Coxa Vara ; Extremities ; Fractures, Multiple ; Genu Varum ; Humans ; Rickets ; Solubility ; Vitamin D ; Wrist

Fracture of intercondylar eminerce of the tibia indicate probable alteration of the cruciate ligament stability in the knee and occur from hyperextension injury of the knee and violent internal rotation of the tibia. Fracture of intercondylar eminence of the tibia has been classified by Meyer and Mckeever(1959, 1970) according to the degree of displacment and this classification is important as it pertains to the proper selection of treatment. The author studied 24 cases in 24 patients of fracture of the intercondylar eminerce of the tibia at the Department of Orthopedic Surgery of Severance Hospital during a 10 year period from Januaary, 1975 until December, 1984. The aim of this study was to find out incidence, cause and associated injury of the fracture of the intercondylar eminece of the tibia and was to asses the comparative results of the conservative and surgical treatment. The result of this study ase as follows ; 1. There is a predisposition for make in a ration of 3:1 and there is no age preponderance in occurance. 2.Majority of cause of injury was traffic accident(62.5%). 3.Isolated fracture of intercondylar eminence of the tibia was 15 cases (62.5%) associated knee joint injury shows 9 cases (37.5%). Among the associated knee joint injury, rupure of medial collateral ligament was 4 cases(44.4%), rupture of medial meniscus was 3 cases(33.3%) and rupture of lateral colateral ligament was 2 cases (22.2%). 4.By Meyer and Mckeever's classification, Type 2(41.7%) fracture of intercondylar eminence of the tibia was most common, and then Type 1(37.5%) and Type 3(20.8%) occured in order of frequency. 5. Type 1 and Type 2 fracture of the intercondylar eminence of the tibia resulted in good prognosis with only conservative treatment, and open reduction is not indicated. 6. Type 3 fracture of the intercondylar eminence of the tibia can be treated with conservative treatment if there is no associated injury of the knee joint.
Classification ; Clinical Study ; Collateral Ligaments ; Equidae ; Humans ; Incidence ; Knee ; Knee Joint ; Ligaments ; Menisci, Tibial ; Orthopedics ; Prognosis ; Rupture ; Tibia

No abstract available.
Femoral Neck Fractures* ; Femur Neck*

The posterior cruciate ligament in the stoutest ligamentous structure in the knee joint. Kennedy and Hawkins have confirmed its strenght to be about 2 times that of the anterior cruciate ligament or tibial collateral ligament. This would appear to offer the support that this ligament has a vital role to play in knee stabilization. But, in the past, non-surgical management was frequently elected either because of the belief that most kness with posterior cruciate insufficiency are asymptomatic or because it is difficult to access surgically. Recently, Hughston and Trickey reported good results after surgical repair of the rupture of the posterior cruciate ligament. And current trend is to repair it surgically. So, we analyzed the 36 cases of acute posterior cruciate ligament injuries, who were admitted and treated surgically in Severance Hospital between Jan. 1981 to Dec. 1985, and obtained the following results. 1. Single rupture of the posterior cruciate ligament largely results in good outcome. 2. When the rupture site is in the substane, we can obtain relatively good results with the augmentation using the medial head of the gastrocnemius. 3. When combined with medial or lateral compartment injuries, the chance of later instability and reconstruction is high.
Anterior Cruciate Ligament ; Clinical Study ; Head ; Knee ; Knee Joint ; Ligaments ; Medial Collateral Ligament, Knee ; Posterior Cruciate Ligament ; Rupture

No abstract available.
Contrast Media* ; Magnetic Resonance Imaging*

PURPOSE: To evaluate the clinical characteristics, treatments and outcome of patients with hemorrhagic shock and encephalopathy(HSE) syndrome. METHODS: We performed a clinical study on 14 patients who were diagnosed as hemorrhagic shock and having encephalopathy syndrome in the Department of Pediatrics, from 1984 to 1998. Age, sex, clinical symptoms and physical findings at admission, the most deranged laboratory findings, radiologic findings, treatments and outcome were analyzed. RESULTS: The age of onset was 1.0+/-0.9 years and the male to female ratio was 1: 1.8. At admission, clinical findings included dehydration in 85.7%, shock in 85.7%, fever in 71.4%, vomiting in 71.4%, diarrhea in 64.3%, GI bleeding in 50%, convulsion in 42.9%, and edema in 35.7%. Altered mental state was found in 100%, hepatomegaly in 64.3%, and splenomegaly in 21.4%. Laboratory findings revealed D-dimer positive in 92.9%, the mean hemoglobin level 8.2+/-2.1g/dL, BUN 35.7+/-24.0mg/dL, creatinine 1.9+/-1.5mg/dL, AST 561.0+/-1,412.1IU/L, ALT 858.9+/-1,649.8IU/L, blood glucose 229.5+/-197.4mg/dL, ammonia 195.4+/-129.7pg/dL, and total bilirubin 4.9+/-8.2mg/dL. On serologic tests, rotavirus and Epstein-Barr virus was found in 1 patient(7.1%), respectively. The mortality rate was 78.6%. CONCLUSION: We found that shock and disseminated intravascular coagulation(DIC) played important roles in the pathogensis of HSE syndrome, and encephalopathy, hepatic and renal insufficiency, and respiratory failure were secondary complications resulting from shock and DIC. Despite vigorous treatment, the prognosis was very poor. We feel more efforts should be focused on investigating the etiology and pathophysiology of HSE to prevent as well as develop a specific therapy. (J Korean Pediatr Soc 2000;43:814-819)
Age of Onset ; Ammonia ; Bilirubin ; Blood Glucose ; Creatinine ; Dacarbazine ; Death, Sudden* ; Dehydration ; Diarrhea ; Edema ; Female ; Fever ; Hemorrhage ; Hepatic Encephalopathy ; Hepatomegaly ; Herpesvirus 4, Human ; Humans ; Infant* ; Male ; Mortality ; Pediatrics ; Prognosis ; Renal Insufficiency ; Respiratory Insufficiency ; Rotavirus ; Seizures ; Serologic Tests ; Shock ; Shock, Hemorrhagic* ; Splenomegaly ; Vomiting