Objective: To study the benzo(a)pyrene (B[a]P)-induced mRNA expression of aromatic hydrocarbon receptor (AHR) and cytochrome P4501A1 (CYP1A1) genes in rat liver. Methods: Rats were injected intraperitoneally with 5, 10 and 15 mg/kg of B[a]P. The total RNAs were extracted from rat livers by RNA purification kit, and the mRNA expression of AHR and CYP1A1 genes was determined by reverse transcription polymerase chain reaction (RT-PCR). β-actin was used as the internal control. The mRNA expression of both AHR and CYP1A1 genes was measured at indicated time points (24, 48 and 72 h) after B[a]P treatment at three different concentrations (5, 10 and 15 mg/kg). Results: The mRNA expression of AHR gene increased in a time-dependent manner at the concentration of 10 mg/kg but not at 5 and 15 mg/kg of B[a]P. The mRNA expression of CYP1A1 gene differed significantly at 48 h and 24 h in rat livers treated with 10 and 15 mg/kg dosage of B[a]P. The mRNA expression of AHR and CYP1A1 genes increased with B[a]P treatment in a concentration-dependent manner. The time-dependent increase in mRNA expression was shown by AHR but not by CYP1A1 gene with B[a]P (10 mg/kg) treatment. Conclusion: This study demonstrates that toxic B[a]P increases the mRNA expression of both AHR and CYP1A1 genes in vivo, suggesting that B[a]P may play a role in cancer genesis by this way.

Objective: To estimate the relative risk for lung cancer associated with genetic polymorphism of T6235C mutation in 3′ non-coding region (MspI) of cytochrome P450 1A1 (CYP1A1) and glutathione S-transferase M1(GSTM1) in the Mongolian population in Inner Mongolian Region of China. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and multiplex PCR methods were used to analyze blood samples obtained from 263 case subjects and 263 control subjects to determine their genotypes for CYP1A1 and GSTM1. Control subjects were matched with case subjects by ethnic background, age and gender. Results: The frequencies of the variant CYP1A1 genotypes (CYP1A1C) and GSTM1-null in lung cancer groups were higher than those in control groups (38.4% vs. 28.5% and 57.8% vs. 48.0%). The individuals who carried with CYP1A1C genotype had a significantly higher risk of lung cancer (OR=1.56, 95% CI=1.08 to 2.25, P=0.016) than those who carried with non-variation CYP1A1 genotype. The ones who carried with GSTM1-null genotype also had a significantly higher risk of lung cancer (OR=1.49, 95% CI=1.06 to 2.10, P=0.023) than those who carried with GSTM1-present genotype. When combination of polymorphisms of CYP1A1 and GSTM1 genotypes was analyzed, the risk of lung cancer for combination of CYP1A1C and GSTM1-null genotypes was increased significantly (OR=2.084, 95% CI=1.27 to 3.42, P=0.003). Susceptibility to lung cancer was related to smoking (OR=2.10, 95% CI=1.48 to 2.98, P=0.000). Considering smoking status, the risk of lung cancer for combination of smoking and CYP1A1C genotype was remarkably increased (OR=2.76, 95% CI=1.74 to 4.37, P=0.000). It was the same case with combination of smoking and GSTM1-null genotype (OR=4.38, 95% CI=2.35 to 8.15, P=0.000). Conclusion: The polymorphisms of CYP1A1C genotype and GSTM1-null are the risk factors of lung cancer in the Mongolian population in Inner Mongolia Region of China. Smoking is also related to susceptibility to lung cancer. There may be a synergetic interaction between CYP1A1C and GSTM1-null in the elevated susceptibility of lung cancer. Smoking may have a synergetic interaction with CYP1A1C and GSTM1-null in the elevated susceptibility of lung cancer.

Objective: To study the benzo(a)pyrene (B[a]P)-induced mRNA expression of aromatic hydrocarbon receptor (AHR) and cytochrome P4501A1 (CYP1A1) genes in rat liver. Methods: Rats were injected intraperitoneally with 5, 10 and 15 mg/kg of B[a]P. The total RNAs were extracted from rat livers by RNA purification kit, and the mRNA expression of AHR and CYP1A1 genes was determined by reverse transcription polymerase chain reaction (RT-PCR). β-actin was used as the internal control. The mRNA expression of both AHR and CYP1A1 genes was measured at indicated time points (24, 48 and 72 h) after B[a]P treatment at three different concentrations (5, 10 and 15 mg/kg). Results: The mRNA expression of AHR gene increased in a time-dependent manner at the concentration of 10 mg/kg but not at 5 and 15 mg/kg of B[a]P. The mRNA expression of CYP1A1 gene differed significantly at 48 h and 24 h in rat livers treated with 10 and 15 mg/kg dosage of B[a]P. The mRNA expression of AHR and CYP1A1 genes increased with B[a]P treatment in a concentration-dependent manner. The time-dependent increase in mRNA expression was shown by AHR but not by CYP1A1 gene with B[a]P (10 mg/kg) treatment. Conclusion: This study demonstrates that toxic B[a]P increases the mRNA expression of both AHR and CYP1A1 genes in vivo, suggesting that B[a]P may play a role in cancer genesis by this way.

Objective: To estimate the relative risk for lung cancer associated with genetic polymorphism of T6235C mutation in 3′ non-coding region (MspI) of cytochrome P450 1A1 (CYP1A1) and glutathione S-transferase M1(GSTM1) in the Mongolian population in Inner Mongolian Region of China. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and multiplex PCR methods were used to analyze blood samples obtained from 263 case subjects and 263 control subjects to determine their genotypes for CYP1A1 and GSTM1. Control subjects were matched with case subjects by ethnic background, age and gender. Results: The frequencies of the variant CYP1A1 genotypes (CYP1A1C) and GSTM1-null in lung cancer groups were higher than those in control groups (38.4% vs. 28.5% and 57.8% vs. 48.0%). The individuals who carried with CYP1A1C genotype had a significantly higher risk of lung cancer (OR=1.56, 95% CI=1.08 to 2.25, P=0.016) than those who carried with non-variation CYP1A1 genotype. The ones who carried with GSTM1-null genotype also had a significantly higher risk of lung cancer (OR=1.49, 95% CI=1.06 to 2.10, P=0.023) than those who carried with GSTM1-present genotype. When combination of polymorphisms of CYP1A1 and GSTM1 genotypes was analyzed, the risk of lung cancer for combination of CYP1A1C and GSTM1-null genotypes was increased significantly (OR=2.084, 95% CI=1.27 to 3.42, P=0.003). Susceptibility to lung cancer was related to smoking (OR=2.10, 95% CI=1.48 to 2.98, P=0.000). Considering smoking status, the risk of lung cancer for combination of smoking and CYP1A1C genotype was remarkably increased (OR=2.76, 95% CI=1.74 to 4.37, P=0.000). It was the same case with combination of smoking and GSTM1-null genotype (OR=4.38, 95% CI=2.35 to 8.15, P=0.000). Conclusion: The polymorphisms of CYP1A1C genotype and GSTM1-null are the risk factors of lung cancer in the Mongolian population in Inner Mongolia Region of China. Smoking is also related to susceptibility to lung cancer. There may be a synergetic interaction between CYP1A1C and GSTM1-null in the elevated susceptibility of lung cancer. Smoking may have a synergetic interaction with CYP1A1C and GSTM1-null in the elevated susceptibility of lung cancer.

Objective To amplify and clone human lrg and to predict its function by bioinformatics analysis. Methods The human lrg was amplified by RT-PCR, then identified by sequencing. Function of human lrg was predicted by bioinformatics analysis with Internet and GenBank database. Results The human lrg was amplified and sequenced correctly. Leucine zipper was found in the human lrg series that may have an important function. Conclusion The human lrg gene has been successfully subcloned and its function has been predicted. The result of the present paper will provide data and evidences for the further study on function of human lrg.

Objective To establish clinical diagnostic criteria for zero-stage non-traumatic osteonecrosis of femoral head,and then to check the accuracy and value of the criteria in clinical application through combined intervention therapy including the administration of traditional Chinese medicine and physical therapy.Methods The clinical data of 258 patients suffering from non-traumatic osteonecrosis of femoral head were retrospectively analyzed.Clinical diagnostic criteria for zero-stage non-traumatic osteonecrosis of femoral head were established based on the analysis.Then a clinical comparative study concerning the criteria was performed,so as to verify its authority.Results The established criteria were proved to be satisfactory.The sensitivity,specificity and accuracy of the criteria were 93.7%,86.4% and 89.7%,respectively.The clinical comparative study showed that the prevalence of non-traumatic osteonecrosis of femoral head in the intervention group(34.2%)was obviously lower than that of the non-intervention group(84.9%,P

0.05). The LDL-C and apoB concentrations in fasting serum in men with 54T allele were significantly higher than those with 54A allele (2.38?0.63 vs 2.21?0.57mmol/L, P

Generalized acrodermatitis continua occurred in a 33-year-old man. Lesions initially limited to the both hands were treated with corticosteroid for long term, however, generalization of the lesions appeared to follow the steroid withdrawal.
Acrodermatitis* ; Adult ; Generalization (Psychology)* ; Hand ; Humans

Objective To observe the expression of acid-sensing ion channel la (ASICla) and to investigate the effect of intracellular Ca2 + concentration on focal cerebral ischemia in diabetic rats.Methods 108 male Wistar rats were divided into three groups:group A [rats with middle cerebral artery occlusion (MCAO)],group B [rats with MCAO and diabetes (DM + MCAO)],group C [rats with MCAO and diabetes treated with fasudil intervention (DM+ MCAO+ fasudil)] (n=36 each).Samples were obtained at the time points of 1,3,6 and 24 h after ischemia respectively (n=9).Models of MCAO and DM+MCAO were prepared.Rats in DM+MCAO+Fasudil group were treated with fasudil 1 mg/Kg by caudal vein injection after half an hour when DM+MCAO model successfully prepared.ASICla expressions were detected at different time points of ischemia in the 3 groups respectively.Ca2+ concentration in ischemia cortex cells were determined at different time points of ischemia in group B and C.Results ASICla expressions were gradually increased along with the ischemia time in group A and B (group A:0.71±0.10,0.80±0.11,0.86±0.08,0.93±0.09;groupB:0.86±0.11,1.05±0.51,2.42±0.08,2.78±0.04; all P＜ 0.05),and ASICla expressions at different time points were higher in group B than in group A (all P＜ 0.05).Ca2-concentration were gradually increased along with the ischemia time in group B (106.32± 18.6,137.84±14.32,151.94± 18.38,183.61±7.96,all P＜0.05).Compared with group B,the levels of ASICla expression and calcium current were reduced in group C.Conclusions The activation of ASICla increases calcium ion flow internal pathway leading to intracellular calcium overload,which may be one of the reasons for the aggravation of focal cerebral ischemia in diabetes.

Objective To compare the differences of lifestyle factors between patients with functional constipation (FC)and constipation-predominant irritable bowel syndrome (IBS-C).Methods From February 2011 to December 2014,255 patients with chronic constipation were enrolled.Among them,there were 170 FC patients and 85 IBS-C patients.At the same period,170 healthy volunteers without symptoms of digestive diseases within one year were recruited as control.The data of demographic information and lifestyle factors were collected.First,single variant analysis was performed for statistical analysis and then the statistically significant variants were analyzed by multivariate logistic regression. Then the factors of FC and IBS-C patients were analyzed by decision tree model and the effects of factors under different categories were analyzed.Results The results of single variant analysis indicated that there was no difference in lifestyle factors between FC group and IBS-C group (all P >0.05).The results of multivariate logistic regression analysis showed that no independent protective or risk factors were found in IBS-C group compared with FC group.According to decision tree model analysis,body mass index (BMI),water intake per day and constipation family history were finally enrolled.The incidence of FC was higher in patients with BMI < 23.56 kg/m2 (except 18.74 to < 19.83 kg/m2 )(79.75 %).The incidence of FC was higher in patients with BMI from 18.74 to <19.83 kg/m2 and water intake <1 L
(66.67%).The incidence of FC was highest in patients with BMI≥23.56 kg/m2 and family history of constipation (70.00%).The total prediction accuracy of this model was 64.6% (42/65 )and area under curve (AUC)value was 0.688.Conclusions FC and IBS-C are related with many lifestyle factors.Low BMI and less water intake per day are influence factors of FC,while higher BMI and family history of constipation are influence factors of IBS-C.