Objective To investigate the effects of the main harmful components in cigarette smoke on the expression of lung cancer susceptibility genes by use of the method of network pharmacology, and to explore the correlations of multiple targets and multiple components and diseases.Methods Literatures about the 11 main tobacco toxic ingredients of cigarette smoke were collected from PubMed and the multicomponent-genes-disease network was structured, and then, shared genes which affect the expression of lung cancer susceptibility were screened out.Then use Cytoscape software to construct the multicomponent-shared genes-disease network.Results Network analysis showed that 11 main harmful components in cigarette smoke influnce 106 lung cancer susceptibility genes, 57 lung cancer susceptibility genes of which were affected by at least 2 of the 11 components.Conclusion From the genetic point of view, the relationship of cigarette smoking and lung cancer was elucidated, and the effect of 11 components on the susceptibility genes of other diseases was also explored.This study may provide some statistical references for further detailed research targeting the relationship of cigarette toxic components and lung cancer genetic susceptibility.

Objective To study the relationship between clinical diagnosis and children ventricular premature beat(VPB) electrocardiogram location and morph.Methods Both organic heart disease and without organic heart disease relationship with 109 cases of children ventricular premature beat electrocardiogram location and morph were retrospectively analyzed.Results Children ventricular premature beat location shows that organic heart disease mostly results from left ventricle, without organic heart disease often comes from right ventricle. There was significant difference between above two groups (? 2=37.25 P

Objective To explore clinic manifestations and laboratory investgation of virus encephalopathy.Methods The clinical course,cerebrospinal fluid(CSF),hepatic dysfunction,computerized tomography of 7 cases treated in our hospital from October 1999 to March 2005 were retrospectively reviewed.Results Seven cases of virus encephalopathy were typically associated with a suddent onset of high fever,severe convulsion,rapidly progressive coma,marked elevations of alanine aminotransferase(AST) and aspartate transaminase(ALT).Four cases died,3 cases had severe sequelae.Blood ammonemia was normal,brain CT scans revealed peripheral or basal nuclei low-density areas.Conclusion Children with a sudden onset of high fever,severe convulsions,rapidly progressive coma may have a poor prognosis.

The key of graduate student cultivation is to improve their science research ability.Here we make a deep analysis of the reason why our students have such a low ability of science research from the school,supervisors and students and then put forward two measures.

AIM： To estabish a HPLC assay for the determination of pirarubicin（Pir） in plasma.METHODS： Daunomycin（DM） was used as the internal standard.Plasma samples were extracted with CHCl3∶ CH3OH（2∶ 1） .0.4M NH4Cl buffer（pH=9.0） solution： CH2OH（1∶ 9） and the internal standard were added.Separation was carried out on a 250mm× 4.6mm（5μ m） phenomenex column with 0.04M KH2PO4（pH=3.0） ∶ CH3CN（68∶ 32） as mobile phase.Fluorescent detector was set at an excitation wavelength of 480nm and an emission wavelength of 550nm.RESULTS： The calibration curves for serum Pir was linear over the range of 5～1 000ng/ml（r=0.9 997） .The recovery of Pir was 95.3％ .The within-day and between-day variations were less than 5％ .T1/2β ， CLs， Vd and AUC of Pir were（12.8± 5.9） h， （128.3± 52.6） L/（m2· h） ， （1 754.3± 478.2） L/m2 and （428.7± 137.2） ng/（h· ml） ， respectively.CONCLUSION： The method is suited for monitoring blood concentration and pharmacokinetic study of Pir.

Objective To investigate the effect of ulinastin on the gastro-intestinal circulation and systemic inflammatory response during open heart surgery with cardiopulmonary bypass( CPB) .Methods Thirty adult patients undergoing valve replacement with mild hypothermic CPB were randomly divided into two groups: ulinastin group (U ,n = 15) and control group (C , n = 15). In ulinastin group patients received ulinastin 6000 IU?kg-1iv after induction of anesthesia and another 6000 IU?kg-1 was added into the priming solution. In control group patients received equal volume of normal saline, instead of ulinastin. The patients were premedicated with morphine 0.2 mg?kg-1 and scopolamine 0.06 mg?kg-1 .Ranitidine 1 mg?kg-1 was given iv before induction of anesthesia. Anesthesia was induced with midazolam 0.1 mg?kg-1 , fentanyl 10?g?kg-1 and vecuronium 0.1 mg?kg-1 and maintained with fentanyl 50-60?g?kg-1, midazolam, isoflurane and vecuroinum. The patients were intubated and mechanically ventilated. PET CO2 was maintained at 35-45 mm Hg during operation. Gastric intramucosal PCO2 (PiCO2 ) was measured (pHi was calculated) and blood concentrations of TNF-?and IL-6 were determined before CPB (T0), 30 min after aorta was cross-clamped (T1), 60 min after termination of CPB(T2 ) and 6 h after operation (T3 ) .Results The two groups were comparable with regard to age, sex, body weight, ejection fraction, duration of CPB and cross-clamping time. (1) pHi decreased significantly at T1-2 as compared with the baseline value at T0 (P

Objective To investigate the effects of ulinastatin ( UTI) on renal function after cardiopulmonary bypass (CPB) .Methods Twenty-four NYHA functional capacity class Ⅱ - Ⅲ patients (15 male, 9 female) aged 24-52 yr, weighing 41-75 kg undergoing valve replacement with CPB were randomly divided into two groups :group ulinastatin (group U, n = 12) and group control (group C, n = 12) . Anesthesia was induced with midazolam 0.1 mg ? kg-1 , fentanyl 10 55% ? kg-1 and vecuronium 0.15 mg ? kg-1 and maintained with intermittent iv boluses of fentanyl, midazolam and vecuronium supplemented with isoflurane inhalation. In group U UTI 300 000 U was added to the priming solution and 300 000U/d was infused iv on the first three days after operation. In group C normal saline was given iv instead of UTI. Blood samples were taken and urine was collected before operation (T0), on the 1st (T,), 3rd (T3), 5th (T5) and 7th day (T7) after operation for determination of serum urea nitrogen (BUN), creatinine (Cr), ? 2-microglobulin (? 2-MG) and urinary ?2-MG, RBP and NAG. Results (1) There were no significant differences between the two groups with respect to age, sex, body weight, CPB time and aortic cross-clamping time. (2) BUN, Cr and serum ? 2-MG levels increased significantly after operation at T1 and/or T3 as compared with the baseline values (T0) in group C and were significantly higher than those in group U at the corresponding time points ( P

OBJECTIVE:To prepare loperamide hydrochloride oral solution,to establish its quality control method,and to study its stability.METHODS:Loperamide hydrochloride oral solution was prepared by using macrogol-400as the anxiliary solvent,water as the solvent,a reversed phase HPLC method was established to determination the content of principal agent loperamide hydrochloride and the method described in Chinese Pharmacopeia was applied to study its stability.RESULTS:A good linear relationship was obtained in the loperamide hydrochloride at concentration of0.05～0.5mg/ml(r=0.9995);The average recoveries of loperamide hydrochloride at the high,medium and low concentrations were100.6%、101.5%and99.1%respectively,with RSD being1.03%、0.49%and0.56%respectively;The stability showed no evident change as compared with before.CONCLUSION:The loperamide hydrochloride oral solution shows the advantages of simple preparation procedure,satisfactory stability and controllable quality.