1.Observation on therapeutic effect of warming needle moxibustion on knee osteoarthritis of deficiency-cold type.
Chang-du LI ; Xin-yong HUANG ; Xu-guang YANG ; Qiong-fen WANG ; Si-qin HUANG
Chinese Acupuncture & Moxibustion 2006;26(3):189-191
OBJECTIVETo compare clinically therapeutic effects of warming needle moxibustion and simple acupuncture on knee osteoarthritis.
METHODSOne hundred and twenty cases conformed with the diagnostic criteria of knee osteoarthritis with TCM kidney-yang deficiency and cold syndrome were randomly divided into a warming needle moxibustion group and a simple acupuncture group. Guanyuan (CV 4), Qihai (CV 6), Zusanli (ST 36) and others were selected in the two groups. Their therapeutic effects were assessed with joint pain scale, knee osteoarthritis severity index scale and cold syndrome differentiation factors.
RESULTSBoth warming needle moxibustion and simple acupuncture could alleviate pain of knee osteoarthritis, improve dysfunction of the knee joint, reduce symptoms. The clinically basic cured rate and the total effective rate were 32.1% and 96.4% in the warming needle moxibustion group, which were better than 7.4% and 92.6% in the simple acupuncture group.
CONCLUSIONWarming needle moxibustion is an effective therapy for knee osteoarthritis with kidney-yang deficiency and cold type.
Acupuncture Therapy ; Humans ; Moxibustion ; Needles ; Osteoarthritis, Knee ; therapy ; Yang Deficiency
2.The effect of n-hexane on the gonad toxicity of female mice.
Jin LIU ; Hui Ling HUANG ; Fen PANG ; Wen Chang ZHANG
Biomedical and Environmental Sciences 2012;25(2):189-196
OBJECTIVETo investigate the toxic effects of n-hexane on the Ganod of female mice.
METHODSn-Hexane was administered to four groups of mice by inhalation at doses of 0, 3.0, 15.1, and 75.8 mL/m3 respectivelyfor five weeks. Each group consisted of 10 mice, of which half were injected in first with 10 IU of pregnant mare serum gonadotrophin (PMSG) on the 33rd days, and then with 10 IU of human chorionic gonadotrophin (HCG) 48 hrs later. After the treatment, mouse sera were sampled and ovulating hormone (LH), follicle-stimulating hormone (FSH), estradiol (E2), and progesterone (P4) levels were measured by electrochemiluminescence immunoassays (ECLIA). In each group, the right ovaries of the non-super-ovulated mice were stained with hematoxylin and eosin while ovaries on the left side were prepared with the TUNEL method in order to detect apoptotic cells.
RESULTSThe duration of the diestrus stage decreased significantly (P < 0.05) in the 75.8 mL/m3 group. All super-ovulated mice in each treatment group produced fewer eggs than those in the control group (P < 0.05). The number of follicles in ovaries in the 75.8 mL/m3 group was smaller compared with the control group (P < 0.05).The serum P4 levels in each treatment group were lower than those in the control group (F = 6.196, P < 0.01). The cell apoptotic rate in the 75.8 mL/m3 group was higher (P < 0.05).
CONCLUSIONn-Hexane may have directly mediated via alterations hormone secretion and promoted granulosal cell apoptotic, which may be one of the important mechanisms for n-hexane induced mouse ovary impairment.
Animals ; Female ; Gonadal Steroid Hormones ; metabolism ; Hexanes ; administration & dosage ; toxicity ; In Situ Nick-End Labeling ; Inhalation Exposure ; Mice ; Ovary ; drug effects
3.Management of Kasabach-Merritt syndrome by drug therapy and surgery.
Hong-Zhao LEI ; Jing HUANG ; Xiao-Fen MENG ; Yu-Chun MA ; Bin SUN ; Jun-Bo QIAO ; Chang-Xian DONG
Chinese Journal of Plastic Surgery 2013;29(2):104-108
OBJECTIVETo summarize the characters of Kasabach-Merritt syndrome (KMS) and to evaluate the therapeutic effect of drug therapy combined with surgery.
METHODSFrom 2004 to 2010, 59 cases with KMS, who underwent drug therapy and surgery, were retrospectively studied. The average age of the patients, including 33 male and 26 female (male/female, 1.269/1), was 2.9 months (range, 7 days-2.5 years). 28 cases with maxillofacial lesions were treated with the ligation of external carotid artery and injection of carbonyldiamide and methylprednisolone. 31 cases with lesions at trunks and extremities were treated by excision of lesions. All the patients were followed up for 2.8 years (range, 6.5 months -7.3 years). Therapeutic outcomes were assessed by evaluating platelet counts,size of lesion, function of trunk and limb.
RESULTS58 cases were cured except for one dead case. Emergency operation was given in 4 cases, and selective operation was performed in other cases (55 cases). The thrombocyte count, hemoglobin and blood coagulation function returned to normal within 1-2 weeks. The mental condition, appetite, body weight,sleeping were greatly improved one week after treatment. The size of the lesions decreased gradually after the management of ligation of external carotid artery including 18 cases within 6-12 months and 10 cases within 13-24 months. Long term follow-up studies indicated that there was no recurrent case, and the weight, height, immunity of the patients with good function activities were in keeping with the normal counterparts.
CONCLUSIONSThe drug combined with surgery therapy is a very reliable management with high curative rate, short disease period and minimum side-effect.
Child, Preschool ; Combined Modality Therapy ; Female ; Humans ; Infant ; Infant, Newborn ; Kasabach-Merritt Syndrome ; drug therapy ; surgery ; therapy ; Male ; Retrospective Studies
4.Malignant transformation of NIH3T3 cells induced by ectopic expression of PC-1 gene.
Xiao-tong CHANG ; Rui-xia LIANG ; Jian-guang ZHOU ; Hao ZHANG ; Jie-zhi LI ; Jian WANG ; Hui ZHANG ; Cui-fen HUANG
Chinese Journal of Pathology 2005;34(1):42-46
OBJECTIVETo establish a mouse fibroblastic cell line stably transfected with PC-1 gene, and using such cell line to investigate tumor development and progression imposed by the ectopic expression of PC-1 gene.
METHODSEukaryotic expression vector pcDNA3.1(-)/myc-his-pc-1 was transfected into mouse fibroblast cell line NIH3T3 by lipofectamine. Stable transfectants were selected by G418. The integration and expression of ectopic PC-1 gene were analyzed by PCR and RT-PCR. Cytomorphological analysis, MTT, soft agar colony formation and nude mice tumorigenesis assay were used to evaluate the effects of PC-1 gene expression on tumor development and progression.
RESULTSNIH 3T3 cell lines stably expressing PC-1 gene were successfully established and confirmed by PCR and RT-PCR analyses of the integration and expression of ectopic PC-1 gene. Comparing with the parental cell line and cells transfected with control vector, the PC-1 gene transfectants acquired several phenotypes of transformed cells: increasing growth rate, ability to grow and form cell colonies on soft agar, and becoming tumorigenic in nude mice.
CONCLUSIONEctopic expression of PC-1 gene in NIH3T3 cells can induce malignant transformation of mouse fibroblastic cells both in vitro and in vivo.
Animals ; Cell Line, Transformed ; Cell Transformation, Neoplastic ; Gene Expression ; Genes, Neoplasm ; physiology ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; NIH 3T3 Cells ; Neoplasm Proteins ; biosynthesis ; genetics ; physiology ; RNA, Messenger ; biosynthesis ; genetics ; Random Allocation ; Transfection
5.Clinical study on combination of acupuncture, cupping and medicine for treatment of fibromyalgia syndrome.
Chang-du LI ; Xiao-ying FU ; Zhen-ya JIANG ; Xu-guang YANG ; Si-qin HUANG ; Qiong-fen WANG ; Jia LIU ; Yuan CHEN
Chinese Acupuncture & Moxibustion 2006;26(1):8-10
OBJECTIVETo observe the therapeutic effect of acupuncture at five mental points and moving cupping on the Hechelu of the back on fibromyalgia syndrome (FS).
METHODSSixty-six cases who conformed to the criteria were randomly divided into the treatment group treated with acupuncture at five mental points, moving cupping on the Hechelu of the back and amitriptyline, and the control group treated with amitriptyline. Clinical therapeutic effects were assessed with McGill Pain Questionnaire (MPQ) and HAMD depression scale.
RESULTSThe therapeutic effect of the treatment group was better than that of the control group with a significant difference between the two groups (P < 0.01).
CONCLUSIONCombination of acupuncture with cupping therapy is an effective therapy for fibromyalgia syndrome.
Acupuncture Points ; Acupuncture Therapy ; Biomedical Research ; Fibromyalgia ; Humans ; Pain Measurement
6.Inhibitory effect of anluohuaxianwan on experimental hepatic fibrosis in rats.
Xing-Hua TAN ; Chang-Qing LI ; Shang-Rong ZOU ; Min XIE ; Ai-Min ZHANG ; Wen-Li LI ; Xiao-Yue LI ; Hui-Fen HUANG ; Chun-Liang LEI
Chinese Journal of Hepatology 2010;18(1):9-12
OBJECTIVETo investigate the effects of anluohuaqianwan on experimental hepatic fibrosis induced by dimethyl nitrosamine (DMN) in rats.
METHODS36 male SD rats were randomly dividied into three groups: model group, normal group, anluohuaqianwan group. The rats in the three groups were treated with DMN daily for 4 weeks. The liver function was detected using auto biochemistry analyzer, the serum HA, LN, IV-C, PIIIP were detected by immunoradiometry, the histopathology was observed in the left liver lobe after HE staining, the expression of matrix metalloproteinase-2 (MMP-2) in liver tissue were detected by immunohistochemistry.
RESULTSThe serum levels of ALT, AST, ALP, TP, ALB and the contents of HA, LN, IV-C in model group were significantly increased compared to these in the normal group (P less than 0.01). The serum levels of ALT, AST and the contents of HA in anluohuaqianwan group were significantly lower than those in the model group (P less than 0.01). The liver MMP-2 in the model group was significantly increased compared to that in the normal group (P less than 0.05). The expression of MMP-2 in liver tissue of model group was lower than that in the anluohuaqianwan group (P less than 0.05).
CONCLUSIONAnluohuaqianwan can inhibit liver fibrosis in rats induced by DMN.
Alanine Transaminase ; blood ; Animals ; Aspartate Aminotransferases ; blood ; Dimethylnitrosamine ; Drug Combinations ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Hyaluronic Acid ; blood ; Hydroxyproline ; metabolism ; Immunohistochemistry ; Liver ; drug effects ; metabolism ; pathology ; Liver Cirrhosis, Experimental ; chemically induced ; drug therapy ; metabolism ; pathology ; Liver Function Tests ; Male ; Matrix Metalloproteinase 2 ; metabolism ; Plants, Medicinal ; chemistry ; Random Allocation ; Rats ; Rats, Sprague-Dawley
7.Comparison of the sleep architecture and symptom of inattention-hyperactivity in children with obstructive sleep apnea hypopnea syndrome before and after surgery.
Zhen-Yun HUANG ; Da-Bo LIU ; Zhi-Bin LI ; Jian-Wen ZHONG ; Jie YU ; Zong-Yu TAN ; Chang-Zhi SUN ; Li-Fen ZHOU ; Qian CHEN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2008;43(3):169-173
OBJECTIVETo observe the symptoms of inattention, hyperactivity among obstructive sleep apnea hypopnea syndrome (OSAHS) children, also to investigate the effects of surgery (tonsillectomy and adenoidectomy or adenoidectomy alone) on the changes of sleep architecture and inattention-hyperactivity score (IHS).
METHODSBetween June 2004 and may 2007, eighty children diagnosed as OSAHS with overnight polysomnography (PSG) were included in this study, only sixteen children had complete pre-op and post-op PSG data. Thirty children with vocal cord nodules were selected as control group. DSM-IV-derived IHS was evaluated by neurologist. All OSAHS children accepted surgery (tonsillectomy and adenoidectomy or adenoidectomy alone) and IHS evaluation. The pre-op and post-op sleep architecture and IHS were compared with that of control group.
RESULTS(1) The median IHS 80 OSAHS children was higher than that it in control group (0.89 vs 0.17) and the difference was significant (Z = -4. 276, P < 0.05). After surgery, it showed a significant reduction in IHS (0.44 vs 0.89, t = 6.219, P < 0.05). (2) Twenty-five OSAHS children had pre-op IHS greater than 1.25 and nine had post-op IHS greater than 1.25, while only three children in control group had IHS greater than 1.25. The difference was statistically significant (chi2 = 5.192, 9.56 respectively, P < 0.05). (3) For sixteen OSAHS children who had both pre-op and post-op PSG data, a decrease in the percentage of phase 1 sleep and an increase in the percentage of phase 2 sleep, slow wave sleep (SWS) and rapid eye movement (REM) sleep were observed in six months after surgery and the difference was significant (t = 12.2, -5.4, -6.3, - 8.1 respectively, P < 0.001). After surgery, apnea-hypopnea index (AHI) decreased from 13.9 times/h to 1.5 times/h while lowest saturation of blood oxygen (LSaO2) increased from 0.855 to 0.940 (t = 5.3, - 3.7 respectively, P < 0.01). REM sleep percentage and LSaO2 was still lower than that of control group six months after surgery.
CONCLUSIONSChildren with OSAHS showed significantly impaired attention and hyperactivity as compared with control group. Improvement of behavior and sleep architecture were observed after adenoidectomy and tonsillectomy.
Attention Deficit Disorder with Hyperactivity ; complications ; diagnosis ; Case-Control Studies ; Child ; Child, Preschool ; Female ; Humans ; Intraoperative Period ; Male ; Sleep Apnea, Obstructive ; complications ; surgery
8.Is Bladder Training by Clamping Before Removal Necessary for Short-Term Indwelling Urinary Catheter Inpatient? A Systematic Review and Meta-analysis.
Li Hsiang WANG ; Ming Fen TSAI ; Chin Yen Stacey HAN ; Yi Chi HUANG ; Hsueh Erh LIU
Asian Nursing Research 2016;10(3):173-181
PURPOSE: Urinary catheterization is a common technique in clinical practice. There is, however, no consensus on management prior to removal of the indwelling catheter for short-term patients. This systematic review examined the necessity of clamping before removal of an indwelling urinary catheter in short-term patients. METHODS: A systematic literature review was conducted using eight databases and predetermined keywords-guided searches. Some 2,515 studies were evaluated. Ten studies that met the inclusion criteria were selected. RESULTS: The quality of the studies was assessed using the Jadad scoring system. Only 40.0% of studies were rated as high quality. This review found that catheter clamping prior to removal was not necessary for the short-term patient. When made a comparison with the unclamping group, there was no significant difference in recatheterization risk, risk of urine retention, patients' subjective perceptions and rate of urinary tract infection. CONCLUSIONS: This review indicated that bladder training by clamping prior to removal of urinary catheters is not necessary in short-term catheter patients. In addition, clamping carries the risk of complications such as prolonging urinary catheter retention and urinary tract injury. Further investigation requires higher quality methodologies and more diverse study designs.
Attitude to Health
;
Catheters, Indwelling
;
Constriction
;
Device Removal
;
Humans
;
Patient Education as Topic/methods
;
Perception
;
Randomized Controlled Trials as Topic
;
Retreatment
;
Urinary Catheterization/*methods
;
Urinary Catheters
;
Urinary Retention/psychology
;
Urinary Tract Infections/therapy
;
Urination/physiology
9.Establishment of immortalized cell lines and genetic stability evaluation for a family with spinocerebellar ataxia type 2.
Ke-qin LIN ; Hao SUN ; Chang-jun ZHANG ; Yu-fen TAO ; Wen YI ; Xiao-qin HUANG ; Li SHI ; Jia-you CHU
Chinese Journal of Medical Genetics 2009;26(4):374-378
OBJECTIVEImmortalized cell lines of spinocerebellar ataxia type 2 (SCA2) with Parkinson disease symptoms were established in order to provide experimental material for future study.
METHODSThe immortalized cell lines were constructed by using Epstein Barr virus and cyclosporine A. Microsatellite markers were detected to see whether there is any change between the cell lines and the original blood samples, and the genetic stability of the cell lines were evaluated.
RESULTSTwenty-five immortalized cell lines were established successfully from the family and the microsatellite markers were unchanged.
CONCLUSIONThe karyotypes of the immortal cell lines were normal and the cell lines were genetically stable.
Adult ; Asian Continental Ancestry Group ; genetics ; Cell Line, Transformed ; Cell Transformation, Viral ; Female ; Herpesvirus 4, Human ; physiology ; Humans ; Karyotyping ; Male ; Microsatellite Repeats ; Pedigree ; Spinocerebellar Ataxias ; genetics ; Young Adult
10.Detection of the JAK2 gene mutation in familial myeloproliferative neoplasm and its clinical significance.
Yong-Min ZHANG ; Li-Xia HAO ; Qi WEI ; Yong-Qiang WEI ; Fen HUANG ; Yu ZHANG ; Chang-Xin YIN ; Ru FENG
Chinese Journal of Hematology 2012;33(2):103-107
OBJECTIVETo comprehend the abnormalities of JAK2, c-mp, EPOR, MPW515L/K and TET2 genes in patients with familial myeloproliferative neoplasm (MPN) and their relatives, and to explore mechanism of MPN pathogenesis.
METHODSThe complete blood counts of 2 brothers diagnosed with MPN in out hospital and their family members (15 persons in together) were performed, and bone marrow (BM) examinations in patients with abnormal blood count were performed PCR, DNA sequencing were used to evaluate the expression of related genes.
RESULTSThe elder brother was diagnosed with essential thrombocythemia (ET), the younger one was polycythemia vera (PV), and others had no clinical manifestation. The third MPN patient was diagnosed based on the blood count and BM examination. The PCR and sequencing results showed that there was JAK2V617F mutation in 3 patients, the elder brother was homozygous, the younger and their father were heterozygous. There were no BCR/ABL fusion gene and c-mp, EPOR, MPW515L/K, TET2 gene mutation in any member. By sequencing the full-length cDNA of familia JAK2 gene, we found that G380A heterozygous mutation was detected in 2 patients, which changed glycine at 127 into aspartic acid, C489T mutation was detected in 13 patients, G2490A mutation in 14, but both of them were synonymous mutations.
CONCLUSIONSJAK2V617F is one of the important indicators to diagnose MPN. The JAK2V617F mutation of this family involves two generations. For newly diagnosed MPN patients, their family members should consider screening, so some familial patients can be diagnosed as early as possible. Gene mutation besides JAK2V627F can be detected by sequencing the full-length of JAK2 gene.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; DNA Mutational Analysis ; Female ; Humans ; Janus Kinase 2 ; genetics ; Male ; Middle Aged ; Myeloproliferative Disorders ; genetics ; Polycythemia Vera ; genetics ; Sequence Analysis, DNA ; Sibling Relations ; Thrombocythemia, Essential ; genetics ; Young Adult