BACKGROUND: Intracellular calcium overload is a common final feature of the ischemic-reperfused heart and mediates the genesis of irreversible cell damage. Reactive oxygen medabolites have been known to play and important role as toxic mediators in myocardial injuries resulting from ischemia and reperfusion. In order to investigate the mechanism of intracellular calcium accumulation in the ischemic-reperfused myocardium, the present study observed the possible contribution of the reactive oxygen metabolite to the calcium transport of cardiac mitochondria. METHODS: Mitochondrial were isolated from rabbit hearts. The effects of a reactive oxygen metabolite, H2O2 on calcium uptake and release, redox states of endogenous pyridine nucleotides and glutathiones of mitochondria respiring with succinate were observed. Calcium uptake and release were monitored by dual-wave length spectrophotometer using a calcium indicator, arsenaze III. Contents and redox states of pyridine nucleotides and glutathiones were measured by enzymatic methods using spectrofluorometer and HPLC. RESULTS: Hydrogen peroxide(10-500microM) promoted calcium release dose-dependently from CA++-preloaded mitochondria, but did not affect the mitochondrial calcium uptake. The H2O2-induced calcium release was accompanied by simultaneous oxidation of the pyridine nucleotides and decrease in the content of the reduced form of glutathione(GSH). When mitochondria were treated with BCNU(N,N=bis(2-chloroethyl)-N-nitrosourea) to inhibit glutathione reductase and so as to reduce the GSH content, there were no increase in calcium release from the mitochondria. These results may indicate that H2O2 increases the permeability of cardiac mitochondrial membrane to calcium in association with the changes in redox state of endogenous pyridine nucleotides, but not with that of glutathiones. CONCLUSION: It is suggested that the reactive oxygen metabolites induce the release of calcium from mitochondria by altering the redox state of pyridine nucleotides, and it may partly be involved in the elevation of cytosolic calcium concentration in the ischemic-reperfused myocardial cells.
Calcium* ; Chromatography, High Pressure Liquid ; Cytosol ; Glutathione Reductase ; Heart ; Hydrogen ; Ischemia ; Mitochondria* ; Mitochondrial Membranes ; Myocardium ; Nucleotides ; Oxidation-Reduction ; Oxygen* ; Permeability ; Reperfusion ; Succinic Acid

No abstract available.
Incidence* ; Nurseries* ; Parenteral Nutrition, Total* ; Pharmacy* ; Sepsis*

Mycosis fungoides(MF) is a cutaneous T-cell lymphoma of low-grade malignancy characterized by the proliferation of small to medium-sized cerebriform lymphoid cells confined to the skin. It usually shows favorable prognosis, but morphologic transformation occurs in some cases and is often associated with a more aggressive clinical course. Herein we report a case of Ki-1 positive large cell lyrnphoma (Ki-1 LCL) which developed in the plaque stage of mycosis fungoides. Although the lesions responded well to low-dose methotrexate therapy and there was no evidence of local or systemic recurrence until now, continuous follow-up is needed because the prognosis of transformed MF is known to be poor compared with primary cutaneous Ki-1 LCL. We also discussed the differential diagnosis of primary cutaneous Ki-1 positive lymphoproliferative disorders.
Diagnosis, Differential ; Follow-Up Studies ; Lymphocytes ; Lymphoma* ; Lymphoma, T-Cell, Cutaneous ; Lymphoproliferative Disorders ; Methotrexate ; Mycosis Fungoides* ; Prognosis ; Recurrence ; Skin

Intramuscular lipoma is an unusual benign mesenchymal tumor, which infiltrates the skeletal muscle, and in exceedingly rare cases can occur on the head or neck. Intramuscular lipoma is composed of mature adipose tissue that infiltrates muscle in a diffuse manner, and the entrapped muscle fibers exhibit varying degrees of atrophic change. Because of infiltrating features of adipocyte, the recurrence rate after excision is very high. We herein report two cases of intramuscular lipoma of the chin.
Adipocytes ; Adipose Tissue ; Chin* ; Head ; Lipoma* ; Muscle, Skeletal ; Neck ; Recurrence

No Abstract Available.
Filtering Surgery* ; Genistein* ; Glaucoma*

The ability of topical corticosteroids to induce an increase in intraocular pressure(IOP) limits the usefullness in ocular disease. To evaluate of the effects of different types of corticosteroids on the IOP, we applied two kinds of corticosteroics to 103 outpatients without intraocular disease. Subjects were randomly divided into two groups. The first group consisted of 67 patients were treated with 0.12% prednisolone. The second group consisted of 36 patients were treated with 0.1% fluorometholone. In the first group, following six weeks of topical application, patients demonstrated significant increase in the IOP (p<0.05). In the second group, no significant changes in the IOP after six weeks of application (p>0.05). In the first group, 72 of 134 eyes(54%) demonstrated increase in the rop. In the second group, 34 of 72 eyes (47%) demonstrated increase in the IOP. The IOP response failed to correlate significantly with sex, age, baseline IOP, refractive error and cup/disc ratio in two different corticosteroids (p>0.05). These findings do emphasize the need to measure the IOP of all patients who undergoing topical steroids application for an extended period.
Adrenal Cortex Hormones* ; Fluorometholone ; Humans ; Intraocular Pressure* ; Outpatients ; Prednisolone ; Refractive Errors ; Steroids

Retroperitoneal fibrosis sometimes causes urological problem involving the ureter, but the mechanism is uncertain. An aortic aneurysm, including an iliac artery aneurysm, is thought to be one of the mechanisms of retroperitoneal fibrosis. However, cases caused by an isolated iliac artery aneurysm are very rare, and symptoms tend to be non-specific; therefore, no definitive treatment has been established. Herein, we report our recent experience of a patient with a left common iliac artery aneurysm involving the ureter, who underwent successful surgical therapy.
Aneurysm* ; Aortic Aneurysm ; Constriction, Pathologic* ; Humans ; Iliac Artery* ; Retroperitoneal Fibrosis* ; Ureter*

No abstract available.
Laryngectomy* ; Lung*

Transscleral and transpupillary diode lasers are methods of cyclophotocoagulation used to lower intraocular pressure by reducing aqueous humor production through ciliary ablation. When compared with the neodymium[Nd]-YAG, the diode laser has characteristics of lower transmission rate through the sclera and more absorption by pigment or melanin in the ciliary body, therefore the transpupillary method might be more effective in Asians who have greater ciliary body pigmentation than caucasian. We compared the efficacy of diode laser transpupillary and transscleral cyclophotocoagulation on lowering of the intraocular pressure in Asian patients. We performed transpupillary[group 2, n=9]and transscleral [group 1, n=21]diode laser cyclophotocoagulation in medically uncontrolled glaucoma patients. Preoperative mean intraocular pressures were 35.0 +/-13.1 millimeters of mercury[group 1]and 31.8 +/-9.8 millimeters of mercury[group 2], respectively. Postoperative mean pressure were 23.0 +/-14.8 millimeters of mercury[group 1]and 24.8 +/-9.9 millimeters of mercury [group 2], respectively. The intraocular pressure decreased in both groups[p=0.002 group 1, p=0.025 group 2]with statistical significance. Complications were phthisis, bullous keratopathy[group 1]and corneal edema[group 2]. Both transscleral and transpupillary diode laser cyclophoto-coagulation may be performed safely to decrease intraocular pressure. Retrospective studies and long term follow up are further needed to determine the safety of laser cyclophotocoagulation.
Absorption ; Aqueous Humor ; Asian Continental Ancestry Group ; Ciliary Body ; Follow-Up Studies ; Glaucoma ; Humans ; Intraocular Pressure ; Lasers, Semiconductor* ; Melanins ; Pigmentation ; Retrospective Studies ; Sclera

STUDY DESIGN: Retrospective study. OBJECTIVES: To conduct an analysis of the union rate and union patterns of local autogenous bone grafts using metal cages, allogenic bone, and local autogenous bone around the cages in posterior lumbar interbody fusion (PLIF). SUMMARY OF LITERATURE REVIEW: Local autologous bone grafting using metal cages is regarded as an effective method for union during PLIF without iliac bone harvesting. There are few studies about the additional bone grafting except using metal cages. MATERIALS AND METHODS: Thirty-nine patients (68 segments) with postoperative computed tomography (CT) results from 6 to 18 months after PLIF were included. We used 2 metal cages filled with local autogenous bone intraoperatively (region C). We also grafted allogenic bone between 2 cages (region A) and local autogenous bone at the side of the cages (region B). Retrospective analyses of the coronal CT sections were performed using the modified Bridwell criteria for assessing the presence of union. A quantitative evaluation was performed using a scoring system that assessed the proportion of radiopaque parts of the union mass. RESULTS: Among the 68 segments, union was observed in 64 segments, resulting in a union rate of 95.6%. The union rates in regions A, B, and C were 86.8%, 89.7%, and 94.1%, respectively. Trabeculation and the quantitative evaluation of union bone showed a statistically significant trend for improvement from regions A to C (p<0.001). CONCLUSIONS: In this study, the complementary effect of additional bone grafting other than local bone grafting was not proven, but it was effective in increasing the fusion area at around 90%. Keeping the height of disc space with the cages, additional bone grafting using local autogenous and allogenous bone may be used as an effective method for stable union.
Bone Transplantation ; Evaluation Studies as Topic ; Humans ; Methods ; Retrospective Studies* ; Transplants*