AIM:To compare the accuracy of conventional contact A-scan and IOL Master in measuring axial length and anterior chamber depth, and to evaluate the characteristics of these two different methods.METHODS:Totally 145 cases (189 eyes) who underwent phacoemulsification and intraocular lens implantation in our hospital from January 2015 to December 2016 were observed prospectively.They were divided into five groups according to ocular axial length measured by IOL Master(Group A:AL≤22mm, Group B:22mm28mm).The axial length and anterior chamber depth were measured by A-scan and IOL Master respectively before operation, corneal curvature was measured by IOL Master.AL≤22mm using Hoffer Q formula to calculate the crystal degree, AL>22mm using Haigis formula to calculate the crystal degree.Analysis of axial length, anterior chamber depth and mean absolute refractive error at 3mo after surgery.RESULTS:The axial length measured by A-scan and IOL-master:Group A were 21.48±0.41mm and 21.46±0.40mm (P>0.05);Group B were 23.13±0.62mm and 23.14±0.63mm(P>0.05);Group C were 25.24±0.56mm and 25.27±0.59mm(P>0.05);Group D were 26.97±0.59mm and 27.03±0.64mm(P>0.05);Group E were 30.76±1.40mm and 31.01±1.53mm(P<0.05).Mean absolute refractive error (MAE) at 3mo after surgery by A-scan and IOL Master:Group A were 0.50±0.30D and 0.43±0.27D(P>0.05);Group B were 0.48±0.34D and 0.45±0.32D(P>0.05);Group C were 0.56±0.32D and 0.49±0.40D(P>0.05);Group D were 0.64±0.16D and 0.50±0.22D(P>0.05);Group E were 0.91±0.47D and 0.62±0.29D(P<0.05), MAE≤±0.50D were 38.7% and 65%(P<0.05), MAE≤±1.00D were 69.2% and 83.3%(P>0.05).Anterior chamber depth measured by A-scan and IOL Master:Group A were 2.81±0.35mm and 2.82±0.41mm (P>0.05);Group B were 3.04±0.50mm and3.10±0.47mm (P>0.05);Group C were 3.55±0.62mm and 3.60±0.52mm (P>0.05);Group D were 3.42±0.24mm and 3.51±0.30mm(P>0.05);Group E were 3.50±0.28mm and 3.61±0.34mm(P>0.05).CONCLUSION:IOL Master and contact A-scan have a high degree of consistency in the biological measurement, IOL Master has higher accuracy for patients with high myopia and long axis.It is a simple, accurate, good-repeatable and non-contact measurement tool.

OBJECTIVE:To study the protective effects of sodium magnesium fructose diphosphate(FDPM)on brain damage induced by ischemiareperfusion in rats.METHODS:Rats were subjected to cerebral ischemia-reperfusion induced by inserting a nylon thread into internal carotid artery to block the origin of middle cerebral artery and removing the thread for recirculation.After1h ischemia,FDPM(400mg/kg),fructose-1,6-diphosphate(FDP,400mg/kg)or magnesium sulfate(MgSO 4 ,30mg/kg)was administrated10min after the onset of ischemia.Neurological scale was scored after1h ischemia and after2h,5h and23h recirculation,and infarction area,MDA content and histopathological change of brain tissue were studied after1h ischemia and after23h recirculation.RESULTS:Compared with model group,400mg/kg FDPM decreased neurological scale,diminished infarction area,reduced MDA content and relieved pathomorphological changes of brain tissue subjected to ischemia-reperfusion in rats.These effects were more powerful than those of FDP(400mg/kg)or MgSO 4 (30mg/kg).CONCL_ USION:FDPM(400mg/kg)could markedly prevent rats from brain damage after cerebral ischemia-reperfusion,and its effects were more potent than those of FDP(400mg/kg)or MgSO 4 (30mg/kg).

Purpose To investigate the expression of Netrin-1 in ovarian serous carcinoma and its c1inicopatho1ogica1 significance. Methods Netrin-1 protein expression was assessed by immunohistochemica1 method in tissue specimens from 20 cases of benign ovari-an serous cystadenomas,13 cases of border1ine ovarian serous neop1asms and 32 cases of ovarian serous carcinomas( OSC). Results The positive proportion of Netrin-1 protein in OSC tissues was significant1y higher than those in border1ine and benign ovarian serous ne-op1asms(P<0. 01). The expression of Netrin-1 in OSC tissues was associated with tumor grade and c1inica1 stage(P<0. 05),but not associated with age,site,tumor size or 1ymph node metastasis( P>0. 05). Kap1an-Meier ana1ysis showed that the 5-year surviva1 rate of patients with Netrin-1 over-expression was significant1y 1ower than that of patients with 1ower expression( P<0. 05 ). Conclu-sions The high expression of Netrin-1 in OSC tissues indicates that Netrin-1 p1ays an important ro1e in cancer pathogenesis and deve1-opment and it may be a new assistant marker for prognosis of OSC.

Adolescent ; Child ; Child, Preschool ; Chronic Disease ; Colonoscopy ; methods ; Diarrhea ; diagnosis ; pathology ; Female ; Humans ; Infant ; Infant, Newborn ; Male

0.05),but in ages(P

Gene targeted mutagenesis and replacement can be used to modify gene sequence in genomic background without position effect or insertion inactivation in transgenic plants. Targeted mutagenesis organism has little biosafety concerns free of transgenes or marker genes. Gene targeted mutagenesis and replacement in high plants now appears to be a potential tool for gene functional analysis in situ, crops genetic improvement and molecular design. Zinc finger nuclease(ZFN)is most important and would be widely used in gene targeted mutagenesis and replacement through introducing double-strand breaks in genome. Strategies for gene targeted mutagenesis and replacement in plants is discussed. ZFN is described in detail from its structure, operation model and application in plants. Developmental prospect of ZFN in plant gene targeted mutagenesis and replacement is also discussed.

We measured the height and length of palpebral fissure, intercanthal distance and upward deviation of lateral canthus, and ovserved presence of double lid and epicanthal fold of 316 children who had no ocular disease except refractive anomaly by the use of standardized photographic method. We also analyzed the results according to the age, sex and the degree of refractive anomaly. The mean of central height of palpebral fissure was 8.0mm and the mean of maximal height was 8.1mm. In most of cases, the point where the height became maximal was at the center of the palpebral fissure and there was no difference accorging to laterality, sex, age and the degree of refractive anomaly. The length of palpebral fissure was 20.0mm in average and there was no difference according to laterality, sex and the degree of refractive anomaly, but it increased with the increasing age. The intercanthal distance was 33.8mm in average and increased with the increasing age but was not influenced by sex and refractive anomaly. The lateral canthus was deviated upward in relation to medial canthus by 5.90 in average and it was more deviated in female than in male. There was no difference according to laterality, age and refractive anomly, The double lid was present in 28.2% of total subject and it was equally frequent in male and female, and more frequent in the older age group. The epicanthal fold was present in 54.7% of total subject and there was no difference accrding to age and sex. Most of them were epicanthus tarsalis(56.1 %) and epicanthus palpebral is type.
Child* ; Female ; Humans ; Male

No abstract available.
Doxorubicin* ; Membrane Potentials* ; Membranes* ; Papillary Muscles*

We report a case of heart failure and pulmonary edema in patient with Graves disease during recovery from general anesthesia for cesarean section. A 34-year-old multigrivida, at 35 weeks gestation underwent emergency cesarian section for preterm labor, twin pregnancy and polyhydramnios. She has been treated with Graves' disease for 5 years. After premedication with propranolol and glycopyrrolate, anesthesia was induced with thiopenthal and maintained with O2 -N2 O-enflurane. At the end of skin closure, heart failure and pulmonary edema were developed. On auscultation, systolic murmur was audible at the left sternal border and immediate echocardiography revealed mitral valve prolapse accompanied with mitral regurgitation. After proper treatment, she was discharged without complication at 14 days after surgery.
Adult ; Anesthesia ; Anesthesia, General ; Auscultation ; Cesarean Section ; Echocardiography ; Emergencies ; Female ; Glycopyrrolate ; Graves Disease ; Heart Failure* ; Heart* ; Humans ; Mitral Valve Insufficiency ; Mitral Valve Prolapse ; Obstetric Labor, Premature ; Polyhydramnios ; Pregnancy ; Pregnancy, Twin ; Premedication ; Propranolol ; Pulmonary Edema* ; Skin ; Systolic Murmurs

No abstract available.
Abscess* ; Bacteria, Aerobic* ; Quinolones* ; Virulence*