No abstract available.
Asthma* ; Child* ; Humans

No abstract available.
Ileus* ; Lymph Nodes*

PURPOSE: Epstein-Barr virus(EBV) exists in the human population in two genetic forms, usually referred to as type 1 and type 2 which have been defined on the basis of sequence divergence in the EBNA-2 and EBNA-3 family genes. In this study, we were intended to investigate whether the subtypes of EBV in malignant lymphoma in Korea were associated with specific disease entities and geographical distribution. MATERIALS AND METHODS: Biopsy samples obtained from 18 Korean patients with malignant lymphoma including Hodgkin's disease(3 cases), B cell lymphoma(1 case), and NK/T cell lymphoma(14 cases) were analyzed to determine the subtype of EBV infected therein. DNA was extracted from formalin-fixed, paraffin-embeded tissues by ordinary method and specific viral sequences were sought using the polymerase chain reaction(PCR) and Southern blot hybridization assay. Oligonucleotide primers used for examination of EBV strain type were derived from the EBNA-3B and EBNA-3C coding regions. As a control, four cases of reactive hyperplasia were analyzed. RESULTS: The two of four reactive hyperplasia cases were associated with type 1 and the rest of two cases with both types. Among the 18 cases with malignant lymphoma, thirteen cases(72%) had type 1, one(6%) had type 2, and four(22%) had dual infections with both types. In case of NK/T cell lymphoma(14 cases) occupying 78% of 18 biopsy samples, 86%(12 cases) were associated with type 1, 7%(1 case) with type 2, and 7%(1 case) with both types. In case of Hodgkin's disease, all of three cases had both types. B cell lymphoma taking only one case of twenty two cases was determined as type 1. CONCLUSION: These observations indicated that type 1 EBV was predominant in Korean patients with malignant lymphoma, especially NK/T cell lymphoma and showed high frequency of dual viral infections(22%) in Hodgkin's disease as well as in reactive hyperplasia.
Biopsy ; Blotting, Southern ; Clinical Coding ; DNA ; DNA Primers ; Genotype ; Herpesvirus 4, Human ; Hodgkin Disease ; Humans ; Hyperplasia ; Korea* ; Lymphoma* ; Lymphoma, B-Cell

This report is a clinical analysis and comparative study with previous report of bronchial asthma at National Medical Center on bronchial asthma in children who were admitted to the MP dept. of national Medical Center from January 1987 to December 1990. The results of the study were as follows: 1) The out-break ratio of bronchial asthma is 3.7% of hospitalized children which is no change that about 4% since 1980. 2) The children under 6 years of ge was 62.7% which among under 3 years was 33.1%. The rate of under 3 year children is increased as previous study. 3) The out-break of bronchial asthma was most common in September and October and so peak incidence was noted in fall and duration of hospitalization was most 2 week. 4) Familial allergic history was positive in 54.6% and bronchial asthma was most common. The presence of allergic past history was 52.2%) and atopic dermatitis was most common 5) Eosinophilia was found in 80.8% and elevated IgE was also in 61.6% of bronchial asthma. 6) We could get positive results of IgE RAST and skin test: D. pteronyssinus, D. farinae, Houst dust. 7) The most frequent findigs of the chest radiograph were within normal limits. 8) The clinical improvement rate of bronchial asthma is 52.5%.
Asthma* ; Child* ; Child, Hospitalized ; Dermatitis, Atopic ; Dust ; Eosinophilia ; Hospitalization ; Humans ; Immunoglobulin E ; Incidence ; Radiography, Thoracic ; Skin Tests

No abstract available.
Tuberculosis*

No abstract available.
Female ; Humans ; Hysterosalpingography*

Cow milk allergy can be defined as an adverse immunologic reactions to cow milk protein. The term is often mistakenly applied to other causes of milk intolerence, such as lactase deficiency and galactosemia, which must be differenciated and excluded. We have experienced 10 children of cow milk allergy at neonatal onset who had suffered from G-I symtoms, such as diarrhea, irritability, weight loss, vomiting and abdominal distension. One half of this patients had family history of allergic diseas. All patients have positive specific IgE RAST to cow's milk protein, milk elimination test and milk challenge test. Brest milk feeding and soy bean formula feeding and soy bean formula feeding have started after diagnosis and then gastrointestinal symtoms and signs of all patients are improved but other allergic diseases are combined in 5 children.
Child ; Diagnosis ; Diarrhea ; Galactosemias ; Humans ; Hypersensitivity* ; Immunoglobulin E ; Infant* ; Lactase ; Milk ; Milk Hypersensitivity ; Milk Proteins ; Soybeans ; Vomiting ; Weight Loss

This study included fifty two premature babies in whom EEG was performed at National Medical Center, from Jan. 1990 to Jun. 1991. clinical and EEG records of all subjects were analyzed and the following results were obtained. 1) The day of first EEG performed were;11 cases within 10 days, 20 cases from 11 days to days, 13 cases from 21 days to 30 days and 8 cases beyond 31 days. 2) The results of first EEG were; within normal limit in 34 cases, mild abnormality in 3 cases, moderate abnormality in 11 cases and marked abnormality in 4 cases. 3) The abnormal EEG ratio according to the sex was higher in female than in male (43.5% vs 27.6%). 4) The abnormal EEG ratio according to the gestational age was higher in shorter gestational age group than in longer gestational age group (40.9% under 32 weeks of G.A. vs 30.0% beyond 33 weeks of G.A.). 5) The abnormal EEG ratio according to the birth weight was not significant. 6) The abnormal EEG ratio according to the ventilator use was higher in used group than in not used group (38.5% vs 30.8%). 7) Combined diseases in 18 cases of abnormal EEG group were jaundice (14 cases), hyaline membrane disease (7 cases), asphyxia (4 cases), anemia (4 cases), hydrocephalus, sepsis, PDA, hypocalcemia, UTI and IVH. 8) The mean apgar score in abnormal EEG group was lower than normal EEG group both in one minute and five minute. 9) Two cases among 18 cases of abnormal EEG group showed abnormal brain sonographic findings, but one case among 34 cases of normal EEG group showed abnormal brain sonographic finding. 10) Ten cases were followed up with serial EEG, six cases of which were treated with anticonvulsant. We observed normalization of the EEG in 5 cases of anticonvulsant treated group.
Anemia ; Apgar Score ; Asphyxia ; Birth Weight ; Brain ; Electroencephalography* ; Female ; Gestational Age ; Humans ; Hyaline Membrane Disease ; Hydrocephalus ; Hypocalcemia ; Infant, Newborn ; Jaundice ; Male ; Sepsis ; Ultrasonography ; Ventilators, Mechanical

No abstract available.
Asthma*

The clinical use of topical antimicrobial substances is common in clean wounds as well as contaminated and chronic wounds. This use is based on the observation that the reduction of superficial bacterial contamination promotes wound healing. While the antibacterial efficacy of topical antimicrobials has been well documented, their effects on living tissue and the process of wound healing remain controversial. Recently, there have been several trials to detect the toxicity of various antimicrobial materials by using cultured fibroblasts and keratinocytes. In this study the cytotoxicity of several commonly used antimicrobials and the cytotoxic concentrations of antimicrobials on cultured human keratinocytes and fibroblasts were compared using 3 different METHODS: 1) the MTT method showing the ability of the cells to clear the cytotoxic agents, 2) LDH release indicating damage of cell membrane, and 3) tritiated thymidine incorporation measuring DNA synthesis. In the MTT method all topical antimicrobials in their usual clinical concentration showed cytotoxicity on both keratinocytes and fibroblasts by suppressing cell proliferation. Keratinocytes were more sensitive to certain antibiotics. When the 50% inhibition concentration (IC50) of topical antimicrobials were compared, the cytotoxicity to keratinocyte was, in the decreasing order, chlorhexidine, hydrogen peroxide, povidone iodine, sulfadiazine, fusidic acid, ethanol. Gentamicin and bacitracin. The cytotoxicity to fibroblasts was, in decreasing order, chiorhexidine, hydrogen peroxide, povidone iodine, fusidic acid, ethanol, sulfadiazine, bacitracin, and gentamicin. Most of the antimicrobials tested increased LDH release by keratinocyte in IC50.Chlorhexidine, hydrogen peroxide and povidone iodine showed statistically significant increase in LDH release. The tritiated thymidine incorporation study showed the order of cytotoxicity to keratinocyte in IC50 of each antimicrobials was similar to that of the MTT method. Tritiated thymidined incorporation, however, appeared to be a more sensitive method in detecting cytotoxicity than MTT because the IC50 was lower in the former. In conclusion, all topical antimicrobials are cytotoxic to keratinocytes and fibroblast in their usual concentration. Because of their cytotoxicity it is suggested that one should use topical antimicrobials in a dilution of up to 1 : 1,000 of commonly used concentration for a short period only especially when applied directly on a skin graft or wounds without a protective barrier. Bacitracin or gentamicin appear to be less cytotoxic among all the antimicrobials tested.
Anti-Bacterial Agents ; Bacitracin ; Cell Membrane ; Cell Proliferation ; Chlorhexidine ; Cytotoxins ; DNA ; Ethanol ; Fibroblasts* ; Fusidic Acid ; Gentamicins ; Humans* ; Hydrogen Peroxide ; Inhibitory Concentration 50 ; Keratinocytes* ; Povidone-Iodine ; Skin ; Sulfadiazine ; Thymidine ; Transplants ; Wound Healing ; Wounds and Injuries